pCMV-VSV-G

Vector for constitutive expression of VSV-G glycoprotein. Also known as pHCMV-G or pHCMV-VSV-G.
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HomePlasmidsViral Expression & Packaging VectorspCMV-VSV-G
XbaI (6502) NotI (6495) SacII (6489) AleI (6488) BssHII (6444) NaeI (6155) NgoMIV (6153) XmnI (5509) NmeAIII (5058) AhdI (4910) PspFI (4325) BseYI (4321) BspQI - SapI (3901) BglII (3195) BsrGI (41) SpeI (97) SnaBI (438) BsmBI (687) SalI (753) AccI (754) PpuMI - SanDI (788) MfeI (1130) PspOMI (1305) ApaI (1309) BamHI (1411) XhoI (1417) SwaI (1572) PasI (2074) EcoNI (2078) XcmI (2184) PstI (2224) AgeI (2417) Acc65I (2444) KpnI (2448) BclI * (2520) BmgBI (2578) Bpu10I (2688) BstBI (2697) XhoI (3097) BamHI (3103) Bsu36I (3125) MscI (3166) pCMV-VSV-G 6507 bp
XbaI  (6502)
1 site		 T C T A G A A G A T C T
NotI  (6495)
1 site		 G C G G C C G C C G C C G G C G
SacII  (6489)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
AleI  (6488)
1 site		 C A C N N N N G T G G T G N N N N C A C
BssHII  (6444)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
NaeI  (6155)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (6153)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
XmnI  (5509)
1 site		 G A A N N N N T T C C T T N N N N A A G
NmeAIII  (5058)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AhdI  (4910)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PspFI  (4325)
1 site		 C C C A G C G G G T C G
BseYI  (4321)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BspQI  (3901)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (3901)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BglII  (3195)
1 site		 A G A T C T T C T A G A
BsrGI  (41)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
SpeI  (97)
1 site		 A C T A G T T G A T C A
SnaBI  (438)
1 site		 T A C G T A A T G C A T
BsmBI  (687)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
SalI  (753)
1 site		 G T C G A C C A G C T G
AccI  (754)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
PpuMI  (788)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
SanDI  (788)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different SanDI sites may not be compatible.
MfeI  (1130)
1 site		 C A A T T G G T T A A C
PspOMI  (1305)
1 site		 G G G C C C C C C G G G
ApaI  (1309)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
BamHI  (1411)
2 sites		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XhoI  (1417)
2 sites		 C T C G A G G A G C T C
SwaI  (1572)
1 site		 A T T T A A A T T A A A T T T A
SwaI is typically used at 25°C, but is 50% active at 37°C.
PasI  (2074)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
EcoNI  (2078)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
XcmI  (2184)
1 site		 C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C
The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
PstI  (2224)
1 site		 C T G C A G G A C G T C
AgeI  (2417)
1 site		 A C C G G T T G G C C A
Acc65I  (2444)
1 site		 G G T A C C C C A T G G
KpnI  (2448)
1 site		 G G T A C C C C A T G G
BclI  (2520)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BmgBI  (2578)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
Bpu10I  (2688)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BstBI  (2697)
1 site		 T T C G A A A A G C T T
XhoI  (3097)
2 sites		 C T C G A G G A G C T C
BamHI  (3103)
2 sites		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Bsu36I  (3125)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
MscI  (3166)
1 site		 T G G C C A A C C G G T
VSV-G
1436 .. 2971  =  1536 bp
511 amino acids  =  57.5 kDa
2 segments
   Segment 1:  signal peptide  
   1436 .. 1483  =  48 bp
   16 amino acids  =  1.8 kDa
Product: vesicular stomatitis virus G glycoprotein
Indiana strain
VSV-G
1436 .. 2971  =  1536 bp
511 amino acids  =  57.5 kDa
2 segments
   Segment 2:  
   1484 .. 2971  =  1488 bp
   495 amino acids  =  55.7 kDa
Product: vesicular stomatitis virus G glycoprotein
Indiana strain
VSV-G
1436 .. 2971  =  1536 bp
511 amino acids  =  57.5 kDa
2 segments
Product: vesicular stomatitis virus G glycoprotein
Indiana strain
AmpR
4837 .. 5697  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4837 .. 5628  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4837 .. 5697  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   5629 .. 5697  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4837 .. 5697  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
4078 .. 4666  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4078 .. 4666  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
β-globin intron
778 .. 1350  =  573 bp
intron from rabbit β-globin gene
β-globin intron
778 .. 1350  =  573 bp
intron from rabbit β-globin gene
f1 ori
5828 .. 6283  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
5828 .. 6283  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
83 .. 462  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
83 .. 462  =  380 bp
human cytomegalovirus immediate early enhancer
CMV promoter
463 .. 666  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
463 .. 666  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AmpR promoter
5698 .. 5802  =  105 bp
AmpR promoter
5698 .. 5802  =  105 bp
β-globin poly(A) signal
3263 .. 3318  =  56 bp
rabbit β-globin polyadenylation signal
β-globin poly(A) signal
3263 .. 3318  =  56 bp
rabbit β-globin polyadenylation signal
T7 promoter
6451 .. 6469  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
6451 .. 6469  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  622 .. 909  =  288 bp
ORF:  95 amino acids  =  11.4 kDa
ORF:  1322 .. 2971  =  1650 bp
ORF:  549 amino acids  =  61.7 kDa
ORF:  4967 .. 5233  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1881 .. 2291  =  411 bp
ORF:  136 amino acids  =  15.2 kDa
ORF:  2392 .. 2640  =  249 bp
ORF:  82 amino acids  =  8.8 kDa
ORF:  4837 .. 5697  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  1604 .. 1882  =  279 bp
ORF:  92 amino acids  =  10.5 kDa
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Download pCMV-VSV-G.dna file

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Individual Sequences & Maps

pAAV-AC-GFP
pLenti6.2 N-Lumio V5-DEST
pLVX-rHom-1
pRetroX-Tet3G
pAAV-AC-Myc-DDK
pLenti6.2 V5-DEST
pLVX-rHom-Nuc1
pRetroX-TetOne
pAAV-AC-RFP
pLenti6.3 TO V5-DEST
pLVX-rHom-Sec1
pRetroX-TetOne-Puro
pAAV2-EF1a-tGFP-WPRE
pLenti6.3 V5-DEST
pLVX-Tet-Off Advanced
pRetroX-Tight-Hyg
pAd BLOCK-iT-DEST
pLenti6.4 R4R2 V5-DEST
pLVX-Tet-On Advanced
pRetroX-Tight-Pur
pAd CMV V5-DEST
pLenti6 BLOCK-iT-DEST
pLVX-Tet3G
pRetroX-TRE3G
pAd PL-DEST
pLenti6 capTEV-CT-DEST
pLVX-TetOne
pRFP-C-RS
pAdenoX-CMV Linear
pLenti6 capTEV-NT-DEST1
pLVX-TetOne-Puro
pRFP-CB-shLenti
pAdenoX-DsRed-Express Linear
pLenti6 TR
pLVX-Tight-Puro
pRS
pAdenoX-PRLS Linear
pLenti6 UbC V5-DEST
pLVX-TRE3G
pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro
pAdenoX-PRLS-DsRed-Express Linear
pLenti6 V5-DEST
pLVX-TRE3G-Hom1
pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro
pAdenoX-PRLS-ZsGreen1 Linear
pLenti7.3 V5-DEST
pLVX-TRE3G-IRES
pRSI12-U6-sh-HTS4-UbiC-TagRFP-2A-Puro
pAdenoX-Tet3G Linear
pLEX-MCS
pLVX-TRE3G-mCherry
pRSI16cb-U6-sh-13kCB18-HTS6-UbiC-TagRFP-2A-Puro
pAdenoX-ZsGreen1 Linear
pLHCX
pLVX-TRE3G-ZsGreen1
pRSI16-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pB-RS
pLKO.1
pLX301
pRSI17cb-U6-sh-13kCB18-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Hygro
pLKO.1 puro
pLX302
pRSI17-U6-sh-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Neo
pLMN-ZsGreen-Neomycin
pLX303
pRSIT12-U6Tet-sh-HTS4-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Puro
pLMPd-Ametrine
pLX304
pRSIT16-U6Tet-sh-HTS6-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Zeo
pLNCX2
pLX304-V5-Blast-Empty
pRSIT17-U6Tet-sh-HTS6-CMV-TetRep-2A-TagGFP2-2A-Puro
pCMV-VSV-G
pLP VSVG
pLXIN
pRSITEP-U6Tet-sh-noHTS-EF1-TetRep-2A-Puro
pGFP-B-RS
pLP1
pLXSN
pRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP
pGFP-C-shLenti
pLP2
pMD2.G
pShuttle2
pGFP-V-RS
pLPCX
pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pSIREN-RetroQ
pGIPZ
pLVX-EF1alpha-IRES-mCherry
pMSCVhyg
pSIREN-RetroQ-DsRed-Express
pLenti-C-GFP
pLVX-EF1alpha-IRES-Puro
pMSCVneo
pSIREN-RetroQ-TetH
pLenti-C-mGFP
pLVX-EF1alpha-IRES-ZsGreen1
pMSCVpuro
pSIREN-RetroQ-TetP
pLenti-C-Myc-DDK
pLVX-EF1alpha-Tet3G
pQC-tTS-IN
pSIREN-RetroQ-ZsGreen1
pLenti-C-Myc-DDK-IRES-BSD
pLVX-Het-1
pQCXIH
psPAX2
pLenti-C-Myc-DDK-IRES-GFP
pLVX-Het-2
pQCXIN
pTRE3G-Hom1
pLenti-C-Myc-DDK-IRES-Neo
pLVX-Het-Mem1
pQCXIP
pTRIPZ
pLenti-C-Myc-DDK-IRES-Puro
pLVX-Het-Nuc1
pQCXIX
ptTS-Neo
pLenti-C-Myc-DDK-IRES-tRFP
pLVX-Hom-1
pRetro-Lib
pZIP-hCMV-RFP-Puro
pLenti-C-tRFP
pLVX-Hom-Mem1
pRetroQ-AcGFP1-C1
pZIP-hCMV-ZSGreen-Puro
pLenti-C-tYFP
pLVX-Hom-Nuc1
pRetroQ-AcGFP1-N1
pZIP-hEF1-alpha-RFP-Puro
pLenti-EF1a-C-mGFP
pLVX-IRES-Hyg
pRetroQ-DsRed-Monomer-C1
pZIP-hEF1-alpha-ZsGreen-Puro
pLenti-EF1a-C-Myc-DDK-IRES-Puro
pLVX-IRES-mCherry
pRetroQ-DsRed-Monomer-N1
pZIP-mCMV-RFP-Puro
pLenti-EF1a-C-tGFP
pLVX-IRES-Neo
pRetroQ-mCherry-C1
pZIP-mCMV-ZsGreen-Puro
pLenti-N-tGFP
pLVX-IRES-Puro
pRetroQ-mCherry-N1
pZIP-mEF1-alpha-RFP-Puro
pLenti-N-tRFP
pLVX-IRES-tdTomato
pRetroX-IRES-DsRedExpress
pZIP-mEF1-alpha-ZsGreen-Puro
pLenti-N-tYFP
pLVX-IRES-ZsGreen1
pRetroX-IRES-ZsGreen1
pZIP-SFFV-RFP-Puro
pLenti3.3 TR
pLVX-PTuner
pRetroX-PTuner
pZIP-SFFV-ZsGreen-Puro
pLenti4 BLOCK-iT-DEST
pLVX-PTuner Green
pRetroX-PTuner IRES
pZIP-TRE3G-ZsGreen-Puro
pLenti4 V5-DEST
pLVX-PTunerC
pRetroX-Tet-Off Advanced
pSIREN-Shuttle (linearized)
pLenti6.2 C-Lumio V5-DEST
pLVX-Puro
pRetroX-Tet-On Advanced
scramble shRNA

Plasmid Sets

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TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
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Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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