pTRE3G-Hom1

Mammalian vector encoding a doxycycline-inducible FKBP homodimerizer domain, for creating soluble fusion proteins that can be dimerized with a drug. 

Sequence Author: Clontech (TaKaRa)

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HomePlasmidsViral Expression & Packaging VectorspTRE3G-Hom1
EcoRI (3770) AatII (3699) ZraI (3697) XmnI (3376) ScaI (3257) TatI (3255) FspI (2999) NmeAIII (2925) BglI (2897) BsrFI (2857) BsaI (2838) AhdI (2777) AlwNI (2300) DrdI (1992) PaeR7I - PspXI - XhoI (1) HindIII (253) KasI (274) NarI (275) SfoI (276) PluTI (278) Eco53kI (292) SacI (294) SalI (383) AccI (384) PspOMI (393) ApaI (397) BglII (399) BspDI * - ClaI * (406) EagI (411) EcoRV (419) ATG PaqCI (431) BsmBI - Esp3I (457) TspMI - XmaI (550) SmaI (552) MluI (756) NdeI (763) NheI (768) BmtI (772) PstI - SbfI (778) BamHI (780) BtgZI (828) PflMI (1002) StyI (1150) BsaBI * (1465) MfeI (1553) HpaI (1566) BspQI - SapI (1768) PciI (1884) pTRE3G-Hom1 3775 bp
EcoRI  (3770)
1 site		 G A A T T C C T T A A G
AatII  (3699)
1 site		 G A C G T C C T G C A G
ZraI  (3697)
1 site		 G A C G T C C T G C A G
XmnI  (3376)
1 site		 G A A N N N N T T C C T T N N N N A A G
ScaI  (3257)
1 site		 A G T A C T T C A T G A
TatI  (3255)
1 site		 W G T A C W W C A T G W
FspI  (2999)
1 site		 T G C G C A A C G C G T
NmeAIII  (2925)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (2897)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
BsrFI  (2857)
1 site		 R C C G G Y Y G G C C R
Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BsaI  (2838)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (2777)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2300)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
DrdI  (1992)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
PaeR7I  (1)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (1)
1 site		 C T C G A G G A G C T C
HindIII  (253)
1 site		 A A G C T T T T C G A A
KasI  (274)
1 site		 G G C G C C C C G C G G
NarI  (275)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (276)
1 site		 G G C G C C C C G C G G
PluTI  (278)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
Eco53kI  (292)
1 site		 G A G C T C C T C G A G
SacI  (294)
1 site		 G A G C T C C T C G A G
SalI  (383)
1 site		 G T C G A C C A G C T G
AccI  (384)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
PspOMI  (393)
1 site		 G G G C C C C C C G G G
ApaI  (397)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
BglII  (399)
1 site		 A G A T C T T C T A G A
BspDI  (406)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (406)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
EagI  (411)
1 site		 C G G C C G G C C G G C
EcoRV  (419)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PaqCI  (431)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
BsmBI  (457)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (457)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
TspMI  (550)
1 site		 C C C G G G G G G C C C
XmaI  (550)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (552)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
MluI  (756)
1 site		 A C G C G T T G C G C A
NdeI  (763)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
NheI  (768)
1 site		 G C T A G C C G A T C G
BmtI  (772)
1 site		 G C T A G C C G A T C G
PstI  (778)
1 site		 C T G C A G G A C G T C
SbfI  (778)
1 site		 C C T G C A G G G G A C G T C C
BamHI  (780)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BtgZI  (828)
1 site		 G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4
Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
PflMI  (1002)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
StyI  (1150)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
BsaBI  (1465)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
MfeI  (1553)
1 site		 C A A T T G G T T A A C
HpaI  (1566)
1 site		 G T T A A C C A A T T G
BspQI  (1768)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (1768)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PciI  (1884)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
AmpR
2704 .. 3564  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2704 .. 3495  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2704 .. 3564  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3496 .. 3564  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2704 .. 3564  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1945 .. 2533  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1945 .. 2533  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
TRE3G promoter
4 .. 382  =  379 bp
3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On® 3G
TRE3G promoter
4 .. 382  =  379 bp
3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On® 3G
ATG
423 .. 425  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
423 .. 425  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
DmrB
435 .. 755  =  321 bp
107 amino acids  =  11.8 kDa
Product: F36V mutant of FK506-binding protein FKBP12
binds synthetic ligands that do not bind wild-type FKBP
DmrB
435 .. 755  =  321 bp
107 amino acids  =  11.8 kDa
Product: F36V mutant of FK506-binding protein FKBP12
binds synthetic ligands that do not bind wild-type FKBP
SV40 poly(A) signal
1567 .. 1688  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1567 .. 1688  =  122 bp
SV40 polyadenylation signal
AmpR promoter
3565 .. 3669  =  105 bp
AmpR promoter
3565 .. 3669  =  105 bp
small t intron
927 .. 992  =  66 bp
simian virus 40 (SV40) small t antigen intron
small t intron
927 .. 992  =  66 bp
simian virus 40 (SV40) small t antigen intron
5' MCS
383 .. 422  =  40 bp
multiple cloning site upstream of DmrB
5' MCS
383 .. 422  =  40 bp
multiple cloning site upstream of DmrB
MCS
756 .. 785  =  30 bp
multiple cloning site
MCS
756 .. 785  =  30 bp
multiple cloning site
tet operator
12 .. 30  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
12 .. 30  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
48 .. 66  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
48 .. 66  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
84 .. 102  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
84 .. 102  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
120 .. 138  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
120 .. 138  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
156 .. 174  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
156 .. 174  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
192 .. 210  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
192 .. 210  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
228 .. 246  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
228 .. 246  =  19 bp
bacterial operator O2 for the tetR and tetA genes
ORF:  35 .. 301  =  267 bp
ORF:  88 amino acids  =  10.5 kDa
ORF:  2834 .. 3100  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  423 .. 788  =  366 bp
ORF:  121 amino acids  =  13.3 kDa
ORF:  1071 .. 1544  =  474 bp
ORF:  157 amino acids  =  18.2 kDa
ORF:  2704 .. 3564  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Individual Sequences & Maps

pAAV-AC-GFP
pLenti6.2 N-Lumio V5-DEST
pLVX-rHom-1
pRetroX-Tet3G
pAAV-AC-Myc-DDK
pLenti6.2 V5-DEST
pLVX-rHom-Nuc1
pRetroX-TetOne
pAAV-AC-RFP
pLenti6.3 TO V5-DEST
pLVX-rHom-Sec1
pRetroX-TetOne-Puro
pAAV2-EF1a-tGFP-WPRE
pLenti6.3 V5-DEST
pLVX-Tet-Off Advanced
pRetroX-Tight-Hyg
pAd BLOCK-iT-DEST
pLenti6.4 R4R2 V5-DEST
pLVX-Tet-On Advanced
pRetroX-Tight-Pur
pAd CMV V5-DEST
pLenti6 BLOCK-iT-DEST
pLVX-Tet3G
pRetroX-TRE3G
pAd PL-DEST
pLenti6 capTEV-CT-DEST
pLVX-TetOne
pRFP-C-RS
pAdenoX-CMV Linear
pLenti6 capTEV-NT-DEST1
pLVX-TetOne-Puro
pRFP-CB-shLenti
pAdenoX-DsRed-Express Linear
pLenti6 TR
pLVX-Tight-Puro
pRS
pAdenoX-PRLS Linear
pLenti6 UbC V5-DEST
pLVX-TRE3G
pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro
pAdenoX-PRLS-DsRed-Express Linear
pLenti6 V5-DEST
pLVX-TRE3G-Hom1
pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro
pAdenoX-PRLS-ZsGreen1 Linear
pLenti7.3 V5-DEST
pLVX-TRE3G-IRES
pRSI12-U6-sh-HTS4-UbiC-TagRFP-2A-Puro
pAdenoX-Tet3G Linear
pLEX-MCS
pLVX-TRE3G-mCherry
pRSI16cb-U6-sh-13kCB18-HTS6-UbiC-TagRFP-2A-Puro
pAdenoX-ZsGreen1 Linear
pLHCX
pLVX-TRE3G-ZsGreen1
pRSI16-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pB-RS
pLKO.1
pLX301
pRSI17cb-U6-sh-13kCB18-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Hygro
pLKO.1 puro
pLX302
pRSI17-U6-sh-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Neo
pLMN-ZsGreen-Neomycin
pLX303
pRSIT12-U6Tet-sh-HTS4-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Puro
pLMPd-Ametrine
pLX304
pRSIT16-U6Tet-sh-HTS6-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Zeo
pLNCX2
pLX304-V5-Blast-Empty
pRSIT17-U6Tet-sh-HTS6-CMV-TetRep-2A-TagGFP2-2A-Puro
pCMV-VSV-G
pLP VSVG
pLXIN
pRSITEP-U6Tet-sh-noHTS-EF1-TetRep-2A-Puro
pGFP-B-RS
pLP1
pLXSN
pRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP
pGFP-C-shLenti
pLP2
pMD2.G
pShuttle2
pGFP-V-RS
pLPCX
pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pSIREN-RetroQ
pGIPZ
pLVX-EF1alpha-IRES-mCherry
pMSCVhyg
pSIREN-RetroQ-DsRed-Express
pLenti-C-GFP
pLVX-EF1alpha-IRES-Puro
pMSCVneo
pSIREN-RetroQ-TetH
pLenti-C-mGFP
pLVX-EF1alpha-IRES-ZsGreen1
pMSCVpuro
pSIREN-RetroQ-TetP
pLenti-C-Myc-DDK
pLVX-EF1alpha-Tet3G
pQC-tTS-IN
pSIREN-RetroQ-ZsGreen1
pLenti-C-Myc-DDK-IRES-BSD
pLVX-Het-1
pQCXIH
psPAX2
pLenti-C-Myc-DDK-IRES-GFP
pLVX-Het-2
pQCXIN
pTRE3G-Hom1
pLenti-C-Myc-DDK-IRES-Neo
pLVX-Het-Mem1
pQCXIP
pTRIPZ
pLenti-C-Myc-DDK-IRES-Puro
pLVX-Het-Nuc1
pQCXIX
ptTS-Neo
pLenti-C-Myc-DDK-IRES-tRFP
pLVX-Hom-1
pRetro-Lib
pZIP-hCMV-RFP-Puro
pLenti-C-tRFP
pLVX-Hom-Mem1
pRetroQ-AcGFP1-C1
pZIP-hCMV-ZSGreen-Puro
pLenti-C-tYFP
pLVX-Hom-Nuc1
pRetroQ-AcGFP1-N1
pZIP-hEF1-alpha-RFP-Puro
pLenti-EF1a-C-mGFP
pLVX-IRES-Hyg
pRetroQ-DsRed-Monomer-C1
pZIP-hEF1-alpha-ZsGreen-Puro
pLenti-EF1a-C-Myc-DDK-IRES-Puro
pLVX-IRES-mCherry
pRetroQ-DsRed-Monomer-N1
pZIP-mCMV-RFP-Puro
pLenti-EF1a-C-tGFP
pLVX-IRES-Neo
pRetroQ-mCherry-C1
pZIP-mCMV-ZsGreen-Puro
pLenti-N-tGFP
pLVX-IRES-Puro
pRetroQ-mCherry-N1
pZIP-mEF1-alpha-RFP-Puro
pLenti-N-tRFP
pLVX-IRES-tdTomato
pRetroX-IRES-DsRedExpress
pZIP-mEF1-alpha-ZsGreen-Puro
pLenti-N-tYFP
pLVX-IRES-ZsGreen1
pRetroX-IRES-ZsGreen1
pZIP-SFFV-RFP-Puro
pLenti3.3 TR
pLVX-PTuner
pRetroX-PTuner
pZIP-SFFV-ZsGreen-Puro
pLenti4 BLOCK-iT-DEST
pLVX-PTuner Green
pRetroX-PTuner IRES
pZIP-TRE3G-ZsGreen-Puro
pLenti4 V5-DEST
pLVX-PTunerC
pRetroX-Tet-Off Advanced
pSIREN-Shuttle (linearized)
pLenti6.2 C-Lumio V5-DEST
pLVX-Puro
pRetroX-Tet-On Advanced
scramble shRNA

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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