pLMN-ZsGreen-Neomycin

Retroviral vector with a ZsGreen marker, for expressing a short hairpin RNA (shRNA) with an "UltramiR" microRNA scaffold.

Sequence Author: transOMIC

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HomePlasmidsViral Expression & Packaging VectorspLMN-ZsGreen-Neomycin
pLMN-ZsGreen-Neomycin 8065 bp
AmpR
6271 .. 7131  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   6271 .. 7062  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6271 .. 7131  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   7063 .. 7131  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6271 .. 7131  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR
2270 .. 3073  =  804 bp
267 amino acids  =  29.3 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
2270 .. 3073  =  804 bp
267 amino acids  =  29.3 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ZsGreen1
3659 .. 4354  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus green fluorescent protein
mammalian codon-optimized
ZsGreen1
3659 .. 4354  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus green fluorescent protein
mammalian codon-optimized
ori
5512 .. 6100  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5512 .. 6100  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
IRES
3096 .. 3647  =  552 bp
internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)
IRES
3096 .. 3647  =  552 bp
internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)
5' LTR
8064 .. 515  =  517 bp
5' long terminal repeat from murine embryonic stem cell virus
5' LTR
8064 .. 515  =  517 bp
5' long terminal repeat from murine embryonic stem cell virus
3' LTR
4458 .. 4972  =  515 bp
3' long terminal repeat from murine embryonic stem cell virus
3' LTR
4458 .. 4972  =  515 bp
3' long terminal repeat from murine embryonic stem cell virus
PGK promoter
1757 .. 2256  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
1757 .. 2256  =  500 bp
mouse phosphoglycerate kinase 1 promoter
gag (truncated)
987 .. 1403  =  417 bp
truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon
gag (truncated)
987 .. 1403  =  417 bp
truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon
MESV Ψ
579 .. 920  =  342 bp
packaging signal of murine embryonic stem cell virus
MESV Ψ
579 .. 920  =  342 bp
packaging signal of murine embryonic stem cell virus
3' miR-30a
1610 .. 1736  =  127 bp
sequence downstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)
3' miR-30a
1610 .. 1736  =  127 bp
sequence downstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)
5' miR-30a
1419 .. 1540  =  122 bp
sequence upstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)
5' miR-30a
1419 .. 1540  =  122 bp
sequence upstream of the 71-nt precursor of the human miR-30a microRNA (Zeng et al., 2002)
AmpR promoter
7132 .. 7236  =  105 bp
AmpR promoter
7132 .. 7236  =  105 bp
shRNA
1541 .. 1609  =  69 bp
3 segments
   Segment 1:  
   1541 .. 1567  =  27 bp
short hairpin RNA
shRNA
1541 .. 1609  =  69 bp
3 segments
   Segment 2:  miR-30a loop  
   1568 .. 1582  =  15 bp
short hairpin RNA
shRNA
1541 .. 1609  =  69 bp
3 segments
   Segment 3:  
   1583 .. 1609  =  27 bp
short hairpin RNA
shRNA
1541 .. 1609  =  69 bp
3 segments
short hairpin RNA
ORF:  2270 .. 3073  =  804 bp
ORF:  267 amino acids  =  29.3 kDa
ORF:  3647 .. 4354  =  708 bp
ORF:  235 amino acids  =  26.5 kDa
ORF:  6401 .. 6667  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  7517 .. 7813  =  297 bp
ORF:  98 amino acids  =  11.7 kDa
ORF:  2037 .. 2450  =  414 bp
ORF:  137 amino acids  =  15.1 kDa
ORF:  2451 .. 2837  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  362 .. 637  =  276 bp
ORF:  91 amino acids  =  9.9 kDa
ORF:  674 .. 934  =  261 bp
ORF:  86 amino acids  =  9.5 kDa
ORF:  2588 .. 2842  =  255 bp
ORF:  84 amino acids  =  9.6 kDa
ORF:  4036 .. 4329  =  294 bp
ORF:  97 amino acids  =  10.9 kDa
ORF:  6271 .. 7131  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  7581 .. 7811  =  231 bp
ORF:  76 amino acids  =  7.5 kDa
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Individual Sequences & Maps

pAAV-AC-GFP
pLenti6.2 N-Lumio V5-DEST
pLVX-rHom-1
pRetroX-Tet3G
pAAV-AC-Myc-DDK
pLenti6.2 V5-DEST
pLVX-rHom-Nuc1
pRetroX-TetOne
pAAV-AC-RFP
pLenti6.3 TO V5-DEST
pLVX-rHom-Sec1
pRetroX-TetOne-Puro
pAAV2-EF1a-tGFP-WPRE
pLenti6.3 V5-DEST
pLVX-Tet-Off Advanced
pRetroX-Tight-Hyg
pAd BLOCK-iT-DEST
pLenti6.4 R4R2 V5-DEST
pLVX-Tet-On Advanced
pRetroX-Tight-Pur
pAd CMV V5-DEST
pLenti6 BLOCK-iT-DEST
pLVX-Tet3G
pRetroX-TRE3G
pAd PL-DEST
pLenti6 capTEV-CT-DEST
pLVX-TetOne
pRFP-C-RS
pAdenoX-CMV Linear
pLenti6 capTEV-NT-DEST1
pLVX-TetOne-Puro
pRFP-CB-shLenti
pAdenoX-DsRed-Express Linear
pLenti6 TR
pLVX-Tight-Puro
pRS
pAdenoX-PRLS Linear
pLenti6 UbC V5-DEST
pLVX-TRE3G
pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro
pAdenoX-PRLS-DsRed-Express Linear
pLenti6 V5-DEST
pLVX-TRE3G-Hom1
pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro
pAdenoX-PRLS-ZsGreen1 Linear
pLenti7.3 V5-DEST
pLVX-TRE3G-IRES
pRSI12-U6-sh-HTS4-UbiC-TagRFP-2A-Puro
pAdenoX-Tet3G Linear
pLEX-MCS
pLVX-TRE3G-mCherry
pRSI16cb-U6-sh-13kCB18-HTS6-UbiC-TagRFP-2A-Puro
pAdenoX-ZsGreen1 Linear
pLHCX
pLVX-TRE3G-ZsGreen1
pRSI16-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pB-RS
pLKO.1
pLX301
pRSI17cb-U6-sh-13kCB18-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Hygro
pLKO.1 puro
pLX302
pRSI17-U6-sh-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Neo
pLMN-ZsGreen-Neomycin
pLX303
pRSIT12-U6Tet-sh-HTS4-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Puro
pLMPd-Ametrine
pLX304
pRSIT16-U6Tet-sh-HTS6-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Zeo
pLNCX2
pLX304-V5-Blast-Empty
pRSIT17-U6Tet-sh-HTS6-CMV-TetRep-2A-TagGFP2-2A-Puro
pCMV-VSV-G
pLP VSVG
pLXIN
pRSITEP-U6Tet-sh-noHTS-EF1-TetRep-2A-Puro
pGFP-B-RS
pLP1
pLXSN
pRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP
pGFP-C-shLenti
pLP2
pMD2.G
pShuttle2
pGFP-V-RS
pLPCX
pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pSIREN-RetroQ
pGIPZ
pLVX-EF1alpha-IRES-mCherry
pMSCVhyg
pSIREN-RetroQ-DsRed-Express
pLenti-C-GFP
pLVX-EF1alpha-IRES-Puro
pMSCVneo
pSIREN-RetroQ-TetH
pLenti-C-mGFP
pLVX-EF1alpha-IRES-ZsGreen1
pMSCVpuro
pSIREN-RetroQ-TetP
pLenti-C-Myc-DDK
pLVX-EF1alpha-Tet3G
pQC-tTS-IN
pSIREN-RetroQ-ZsGreen1
pLenti-C-Myc-DDK-IRES-BSD
pLVX-Het-1
pQCXIH
psPAX2
pLenti-C-Myc-DDK-IRES-GFP
pLVX-Het-2
pQCXIN
pTRE3G-Hom1
pLenti-C-Myc-DDK-IRES-Neo
pLVX-Het-Mem1
pQCXIP
pTRIPZ
pLenti-C-Myc-DDK-IRES-Puro
pLVX-Het-Nuc1
pQCXIX
ptTS-Neo
pLenti-C-Myc-DDK-IRES-tRFP
pLVX-Hom-1
pRetro-Lib
pZIP-hCMV-RFP-Puro
pLenti-C-tRFP
pLVX-Hom-Mem1
pRetroQ-AcGFP1-C1
pZIP-hCMV-ZSGreen-Puro
pLenti-C-tYFP
pLVX-Hom-Nuc1
pRetroQ-AcGFP1-N1
pZIP-hEF1-alpha-RFP-Puro
pLenti-EF1a-C-mGFP
pLVX-IRES-Hyg
pRetroQ-DsRed-Monomer-C1
pZIP-hEF1-alpha-ZsGreen-Puro
pLenti-EF1a-C-Myc-DDK-IRES-Puro
pLVX-IRES-mCherry
pRetroQ-DsRed-Monomer-N1
pZIP-mCMV-RFP-Puro
pLenti-EF1a-C-tGFP
pLVX-IRES-Neo
pRetroQ-mCherry-C1
pZIP-mCMV-ZsGreen-Puro
pLenti-N-tGFP
pLVX-IRES-Puro
pRetroQ-mCherry-N1
pZIP-mEF1-alpha-RFP-Puro
pLenti-N-tRFP
pLVX-IRES-tdTomato
pRetroX-IRES-DsRedExpress
pZIP-mEF1-alpha-ZsGreen-Puro
pLenti-N-tYFP
pLVX-IRES-ZsGreen1
pRetroX-IRES-ZsGreen1
pZIP-SFFV-RFP-Puro
pLenti3.3 TR
pLVX-PTuner
pRetroX-PTuner
pZIP-SFFV-ZsGreen-Puro
pLenti4 BLOCK-iT-DEST
pLVX-PTuner Green
pRetroX-PTuner IRES
pZIP-TRE3G-ZsGreen-Puro
pLenti4 V5-DEST
pLVX-PTunerC
pRetroX-Tet-Off Advanced
pSIREN-Shuttle (linearized)
pLenti6.2 C-Lumio V5-DEST
pLVX-Puro
pRetroX-Tet-On Advanced
scramble shRNA

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