pLVX-EF1alpha-Tet3G

Lentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression.

Sequence Author: Clontech (TaKaRa)

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HomePlasmidsViral Expression & Packaging VectorspLVX-EF1alpha-Tet3G
5ʹ 3ʹ 80 5' LTR T G G A A G G G C T A A T T C A C T C C C A A A G A A G A C A A G A T A T C C T T G A T C T G T G G A T C T A C C A C A C A C A A G G C T A C T T C C C T G A T A C C T T C C C G A T T A A G T G A G G G T T T C T T C T G T T C T A T A G G A A C T A G A C A C C T A G A T G G T G T G T G T T C C G A T G A A G G G A C T A

5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
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160 5' LTR T A G C A G A A C T A C A C A C C A G G G C C A G G G G T C A G A T A T C C A C T G A C C T T T G G A T G G T G C T A C A A G C T A G T A C C A G T T G A G C C A T C G T C T T G A T G T G T G G T C C C G G T C C C C A G T C T A T A G G T G A C T G G A A A C C T A C C A C G A T G T T C G A T C A T G G T C A A C T C G G

5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
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240 5' LTR A G A T A A G G T A G A A G A G G C C A A T A A A G G A G A G A A C A C C A G C T T G T T A C A C C C T G T G A G C C T G C A T G G G A T G G A T G A C C C G G T C T A T T C C A T C T T C T C C G G T T A T T T C C T C T C T T G T G G T C G A A C A A T G T G G G A C A C T C G G A C G T A C C C T A C C T A C T G G G C C

5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
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320 5' LTR A G A G A G A A G T G T T A G A G T G G A G G T T T G A C A G C C G C C T A G C A T T T C A T C A C G T G G C C C G A G A G C T G C A T C C G G A G T A C T T C T C T C T C T T C A C A A T C T C A C C T C C A A A C T G T C G G C G G A T C G T A A A G T A G T G C A C C G G G C T C T C G A C G T A G G C C T C A T G A A G

5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
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400 5' LTR A A G A A C T G C T G A T A T C G A G C T T G C T A C A A G G G A C T T T C C G C T G G G G A C T T T C C A G G G A G G C G T G G C C T G G G C G G G A C T G G T T C T T G A C G A C T A T A G C T C G A A C G A T G T T C C C T G A A A G G C G A C C C C T G A A A G G T C C C T C C G C A C C G G A C C C G C C C T G A C C

5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
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480 5' LTR G G A G T G G C G A G C C C T C A G A T C C T G C A T A T A A G C A G C T G C T T T T T G C C T G T A C T G G G T C T C T C T G G T T A G A C C A G A T C T G A C C T C A C C G C T C G G G A G T C T A G G A C G T A T A T T C G T C G A C G A A A A A C G G A C A T G A C C C A G A G A G A C C A A T C T G G T C T A G A C T

5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
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560 5' LTR G C C T G G G A G C T C T C T G G C T A A C T A G G G A A C C C A C T G C T T A A G C C T C A A T A A A G C T T G C C T T G A G T G C T T C A A G T A G T G T G C G G A C C C T C G A G A G A C C G A T T G A T C C C T T G G G T G A C G A A T T C G G A G T T A T T T C G A A C G G A A C T C A C G A A G T T C A T C A C A C

5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
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640 5' LTR T G C C C G T C T G T T G T G T G A C T C T G G T A A C T A G A G A T C C C T C A G A C C C T T T T A G T C A G T G T G G A A A A T C T C T A G C A G T G G C G A C G G G C A G A C A A C A C A C T G A G A C C A T T G A T C T C T A G G G A G T C T G G G A A A A T C A G T C A C A C C T T T T A G A G A T C G T C A C C G C

5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
5' LTR
1 .. 634  =  634 bp
5' long terminal repeat (LTR) from HIV-1
Click here to try SnapGene
720 HIV-1 ψ C C C G A A C A G G G A C T T G A A A G C G A A A G G G A A A C C A G A G G A G C T C T C T C G A C G C A G G A C T C G G C T T G C T G A A G C G C G C A C G G G G G C T T G T C C C T G A A C T T T C G C T T T C C C T T T G G T C T C C T C G A G A G A G C T G C G T C C T G A G C C G A A C G A C T T C G C G C G T G C C

HIV-1 ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
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800 HIV-1 ψ C A A G A G G C G A G G G G C G G C G A C T G G T G A G T A C G C C A A A A A T T T T G A C T A G C G G A G G C T A G A A G G A G A G A G A T G G G T G C G A G G T T C T C C G C T C C C C G C C G C T G A C C A C T C A T G C G G T T T T T A A A A C T G A T C G C C T C C G A T C T T C C T C T C T C T A C C C A C G C T C

HIV-1 ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
Click here to try SnapGene
880 HIV-1 ψ A G C G T C A G T A T T A A G C G G G G G A G A A T T A G A T C G C G A T G G G A A A A A A T T C G G T T A A G G C C A G G G G G A A A G A A A A A A T A T A A T C G C A G T C A T A A T T C G C C C C C T C T T A A T C T A G C G C T A C C C T T T T T T A A G C C A A T T C C G G T C C C C C T T T C T T T T T T A T A T T

HIV-1 ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
Click here to try SnapGene
960 A T T A A A A C A T A T A G T A T G G G C A A G C A G G G A G C T A G A A C G A T T C G C A G T T A A T C C T G G C C T G T T A G A A A C A T C A G A A G G C T T A A T T T T G T A T A T C A T A C C C G T T C G T C C C T C G A T C T T G C T A A G C G T C A A T T A G G A C C G G A C A A T C T T T G T A G T C T T C C G A

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1040 G T A G A C A A A T A C T G G G A C A G C T A C A A C C A T C C C T T C A G A C A G G A T C A G A A G A A C T T A G A T C A T T A T A T A A T A C A G T A G C A C A T C T G T T T A T G A C C C T G T C G A T G T T G G T A G G G A A G T C T G T C C T A G T C T T C T T G A A T C T A G T A A T A T A T T A T G T C A T C G T

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1120 A C C C T C T A T T G T G T G C A T C A A A G G A T A G A G A T A A A A G A C A C C A A G G A A G C T T T A G A C A A G A T A G A G G A A G A G C A A A A C A A T G G G A G A T A A C A C A C G T A G T T T C C T A T C T C T A T T T T C T G T G G T T C C T T C G A A A T C T G T T C T A T C T C C T T C T C G T T T T G T T

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1200 A A G T A A G A C C A C C G C A C A G C A A G C G G C C G G C C G C T G A T C T T C A G A C C T G G A G G A G G A G A T A T G A G G G A C A A T T G G A G A A G T T C A T T C T G G T G G C G T G T C G T T C G C C G G C C G G C G A C T A G A A G T C T G G A C C T C C T C C T C T A T A C T C C C T G T T A A C C T C T T C

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Download pLVX-EF1alpha-Tet3G.dna file

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Individual Sequences & Maps

pAAV-AC-GFP
pLenti6.2 N-Lumio V5-DEST
pLVX-rHom-1
pRetroX-Tet3G
pAAV-AC-Myc-DDK
pLenti6.2 V5-DEST
pLVX-rHom-Nuc1
pRetroX-TetOne
pAAV-AC-RFP
pLenti6.3 TO V5-DEST
pLVX-rHom-Sec1
pRetroX-TetOne-Puro
pAAV2-EF1a-tGFP-WPRE
pLenti6.3 V5-DEST
pLVX-Tet-Off Advanced
pRetroX-Tight-Hyg
pAd BLOCK-iT-DEST
pLenti6.4 R4R2 V5-DEST
pLVX-Tet-On Advanced
pRetroX-Tight-Pur
pAd CMV V5-DEST
pLenti6 BLOCK-iT-DEST
pLVX-Tet3G
pRetroX-TRE3G
pAd PL-DEST
pLenti6 capTEV-CT-DEST
pLVX-TetOne
pRFP-C-RS
pAdenoX-CMV Linear
pLenti6 capTEV-NT-DEST1
pLVX-TetOne-Puro
pRFP-CB-shLenti
pAdenoX-DsRed-Express Linear
pLenti6 TR
pLVX-Tight-Puro
pRS
pAdenoX-PRLS Linear
pLenti6 UbC V5-DEST
pLVX-TRE3G
pRSGC1-U6-sg-HTS6-CMV-Cas9-2A-Puro
pAdenoX-PRLS-DsRed-Express Linear
pLenti6 V5-DEST
pLVX-TRE3G-Hom1
pRSGC2-U6-sg-HTS6-UbiC-Cas9-2A-Puro
pAdenoX-PRLS-ZsGreen1 Linear
pLenti7.3 V5-DEST
pLVX-TRE3G-IRES
pRSI12-U6-sh-HTS4-UbiC-TagRFP-2A-Puro
pAdenoX-Tet3G Linear
pLEX-MCS
pLVX-TRE3G-mCherry
pRSI16cb-U6-sh-13kCB18-HTS6-UbiC-TagRFP-2A-Puro
pAdenoX-ZsGreen1 Linear
pLHCX
pLVX-TRE3G-ZsGreen1
pRSI16-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pB-RS
pLKO.1
pLX301
pRSI17cb-U6-sh-13kCB18-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Hygro
pLKO.1 puro
pLX302
pRSI17-U6-sh-HTS6-UbiC-TagGFP2-2A-Puro
pBABE-Neo
pLMN-ZsGreen-Neomycin
pLX303
pRSIT12-U6Tet-sh-HTS4-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Puro
pLMPd-Ametrine
pLX304
pRSIT16-U6Tet-sh-HTS6-CMV-TetRep-2A-TagRFP-2A-Puro
pBABE-Zeo
pLNCX2
pLX304-V5-Blast-Empty
pRSIT17-U6Tet-sh-HTS6-CMV-TetRep-2A-TagGFP2-2A-Puro
pCMV-VSV-G
pLP VSVG
pLXIN
pRSITEP-U6Tet-sh-noHTS-EF1-TetRep-2A-Puro
pGFP-B-RS
pLP1
pLXSN
pRSITUR-U6Tet-sh-HTS3-UbiC-TetRep-2A-TagRFP
pGFP-C-shLenti
pLP2
pMD2.G
pShuttle2
pGFP-V-RS
pLPCX
pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro
pSIREN-RetroQ
pGIPZ
pLVX-EF1alpha-IRES-mCherry
pMSCVhyg
pSIREN-RetroQ-DsRed-Express
pLenti-C-GFP
pLVX-EF1alpha-IRES-Puro
pMSCVneo
pSIREN-RetroQ-TetH
pLenti-C-mGFP
pLVX-EF1alpha-IRES-ZsGreen1
pMSCVpuro
pSIREN-RetroQ-TetP
pLenti-C-Myc-DDK
pLVX-EF1alpha-Tet3G
pQC-tTS-IN
pSIREN-RetroQ-ZsGreen1
pLenti-C-Myc-DDK-IRES-BSD
pLVX-Het-1
pQCXIH
psPAX2
pLenti-C-Myc-DDK-IRES-GFP
pLVX-Het-2
pQCXIN
pTRE3G-Hom1
pLenti-C-Myc-DDK-IRES-Neo
pLVX-Het-Mem1
pQCXIP
pTRIPZ
pLenti-C-Myc-DDK-IRES-Puro
pLVX-Het-Nuc1
pQCXIX
ptTS-Neo
pLenti-C-Myc-DDK-IRES-tRFP
pLVX-Hom-1
pRetro-Lib
pZIP-hCMV-RFP-Puro
pLenti-C-tRFP
pLVX-Hom-Mem1
pRetroQ-AcGFP1-C1
pZIP-hCMV-ZSGreen-Puro
pLenti-C-tYFP
pLVX-Hom-Nuc1
pRetroQ-AcGFP1-N1
pZIP-hEF1-alpha-RFP-Puro
pLenti-EF1a-C-mGFP
pLVX-IRES-Hyg
pRetroQ-DsRed-Monomer-C1
pZIP-hEF1-alpha-ZsGreen-Puro
pLenti-EF1a-C-Myc-DDK-IRES-Puro
pLVX-IRES-mCherry
pRetroQ-DsRed-Monomer-N1
pZIP-mCMV-RFP-Puro
pLenti-EF1a-C-tGFP
pLVX-IRES-Neo
pRetroQ-mCherry-C1
pZIP-mCMV-ZsGreen-Puro
pLenti-N-tGFP
pLVX-IRES-Puro
pRetroQ-mCherry-N1
pZIP-mEF1-alpha-RFP-Puro
pLenti-N-tRFP
pLVX-IRES-tdTomato
pRetroX-IRES-DsRedExpress
pZIP-mEF1-alpha-ZsGreen-Puro
pLenti-N-tYFP
pLVX-IRES-ZsGreen1
pRetroX-IRES-ZsGreen1
pZIP-SFFV-RFP-Puro
pLenti3.3 TR
pLVX-PTuner
pRetroX-PTuner
pZIP-SFFV-ZsGreen-Puro
pLenti4 BLOCK-iT-DEST
pLVX-PTuner Green
pRetroX-PTuner IRES
pZIP-TRE3G-ZsGreen-Puro
pLenti4 V5-DEST
pLVX-PTunerC
pRetroX-Tet-Off Advanced
pSIREN-Shuttle (linearized)
pLenti6.2 C-Lumio V5-DEST
pLVX-Puro
pRetroX-Tet-On Advanced
scramble shRNA

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