pE-SUMOpro Kan

Bacterial vector with a kanamycin resistance marker, for regulated expression of recombinant proteins with a cleavable 6xHis-SUMO tag.

Sequence Author: LifeSensors

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HomePlasmidsBasic Cloning VectorspE-SUMOpro Kan
SgrAI (5588) SphI (5440) BstAPI (5231) MluI (4907) BstEII (4725) NmeAIII (4707) ApaI (4704) PspOMI (4700) BssHII (4496) EcoRV (4461) HpaI (4405) PshAI (4066) BglI (3850) FspI - FspAI (3829) PflFI - Tth111I (3064) BstZ17I (3039) TatI (3002) BspQI - SapI (2923) T7 promoter XbaI (67) RBS NcoI (106) ATG 6xHis KflI (133) PstI - SbfI (142) DraI (214) AflII (323) BsaI (427) SpeI (439) Acc65I (445) AgeI (448) KpnI (449) BsaI (457) BamHI (463) Eco53kI (477) SacI (479) SalI (482) HindIII (488) EagI - NotI (495) PaeR7I - PspXI - XhoI (503) 6xHis DraIII (910) PsiI (1035) AsiSI - PvuI (1610) TspMI - XmaI (1732) SmaI (1734) BspDI - ClaI (1915) NruI (1951) AlwNI (2397) BssSαI (2633) PciI (2806) pE-SUMOpro Kan 5628 bp
SgrAI  (5588)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SphI  (5440)
1 site		 G C A T G C C G T A C G
BstAPI  (5231)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
MluI  (4907)
1 site		 A C G C G T T G C G C A
BstEII  (4725)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
NmeAIII  (4707)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
ApaI  (4704)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
PspOMI  (4700)
1 site		 G G G C C C C C C G G G
BssHII  (4496)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
EcoRV  (4461)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
HpaI  (4405)
1 site		 G T T A A C C A A T T G
PshAI  (4066)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BglI  (3850)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
FspI  (3829)
1 site		 T G C G C A A C G C G T
FspAI  (3829)
1 site		 R T G C G C A Y Y A C G C G T R
PflFI  (3064)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3064)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BstZ17I  (3039)
1 site		 G T A T A C C A T A T G
TatI  (3002)
1 site		 W G T A C W W C A T G W
BspQI  (2923)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (2923)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
XbaI  (67)
1 site		 T C T A G A A G A T C T
NcoI  (106)
1 site		 C C A T G G G G T A C C
KflI  (133)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different KflI sites may not be compatible.
PstI  (142)
1 site		 C T G C A G G A C G T C
SbfI  (142)
1 site		 C C T G C A G G G G A C G T C C
DraI  (214)
1 site		 T T T A A A A A A T T T
AflII  (323)
1 site		 C T T A A G G A A T T C
BsaI  (427)
2 sites		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
SpeI  (439)
1 site		 A C T A G T T G A T C A
Acc65I  (445)
1 site		 G G T A C C C C A T G G
AgeI  (448)
1 site		 A C C G G T T G G C C A
KpnI  (449)
1 site		 G G T A C C C C A T G G
BsaI  (457)
2 sites		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BamHI  (463)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Eco53kI  (477)
1 site		 G A G C T C C T C G A G
SacI  (479)
1 site		 G A G C T C C T C G A G
SalI  (482)
1 site		 G T C G A C C A G C T G
HindIII  (488)
1 site		 A A G C T T T T C G A A
EagI  (495)
1 site		 C G G C C G G C C G G C
NotI  (495)
1 site		 G C G G C C G C C G C C G G C G
PaeR7I  (503)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (503)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (503)
1 site		 C T C G A G G A G C T C
DraIII  (910)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
PsiI  (1035)
1 site		 T T A T A A A A T A T T
AsiSI  (1610)
1 site		 G C G A T C G C C G C T A G C G
PvuI  (1610)
1 site		 C G A T C G G C T A G C
TspMI  (1732)
1 site		 C C C G G G G G G C C C
XmaI  (1732)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (1734)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
BspDI  (1915)
1 site		 A T C G A T T A G C T A
ClaI  (1915)
1 site		 A T C G A T T A G C T A
NruI  (1951)
1 site		 T C G C G A A G C G C T
AlwNI  (2397)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
BssSαI  (2633)
1 site		 C A C G A G G T G C T C
PciI  (2806)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
lacI
4180 .. 5262  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
4180 .. 5262  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
KanR
1225 .. 2040  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes
KanR
1225 .. 2040  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes
ori
2162 .. 2750  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2162 .. 2750  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
677 .. 1132  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
677 .. 1132  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
ATG
108 .. 110  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
108 .. 110  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
6xHis
114 .. 131  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
114 .. 131  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
SUMO
144 .. 431  =  288 bp
96 amino acids  =  11.0 kDa
Product: cleavable ubiquitin-like protein tag
SUMO
144 .. 431  =  288 bp
96 amino acids  =  11.0 kDa
Product: cleavable ubiquitin-like protein tag
rop
3180 .. 3371  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein, which maintains plasmids at low copy number
rop
3180 .. 3371  =  192 bp
63 amino acids  =  7.2 kDa
Product: Rop protein, which maintains plasmids at low copy number
lacI promoter
5263 .. 5340  =  78 bp
lacI promoter
5263 .. 5340  =  78 bp
MCS
439 .. 508  =  70 bp
multiple cloning site
MCS
439 .. 508  =  70 bp
multiple cloning site
T7 terminator
593 .. 640  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
593 .. 640  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
lac operator
40 .. 64  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
40 .. 64  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
RBS
79 .. 101  =  23 bp
efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
RBS
79 .. 101  =  23 bp
efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)
T7 promoter
21 .. 39  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
21 .. 39  =  19 bp
promoter for bacteriophage T7 RNA polymerase
6xHis
509 .. 526  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
509 .. 526  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
ORF:  3403 .. 3771  =  369 bp
ORF:  122 amino acids  =  14.2 kDa
ORF:  5438 .. 49  =  240 bp
ORF:  79 amino acids  =  8.0 kDa
ORF:  108 .. 434  =  327 bp
ORF:  108 amino acids  =  12.4 kDa
ORF:  4146 .. 4397  =  252 bp
ORF:  83 amino acids  =  9.1 kDa
ORF:  5301 .. 5564  =  264 bp
ORF:  87 amino acids  =  9.5 kDa
ORF:  1225 .. 2040  =  816 bp
ORF:  271 amino acids  =  31.0 kDa
ORF:  4180 .. 5139  =  960 bp
ORF:  319 amino acids  =  34.1 kDa
ORF:  3180 .. 3404  =  225 bp
ORF:  74 amino acids  =  8.5 kDa
ORF:  3768 .. 4124  =  357 bp
ORF:  118 amino acids  =  13.0 kDa
ORF:  4160 .. 4423  =  264 bp
ORF:  87 amino acids  =  8.9 kDa
ORF:  5012 .. 5512  =  501 bp
ORF:  166 amino acids  =  17.5 kDa
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Individual Sequences & Maps

BlueScribe
pEASY-T1 (linearized)
pJET1.2
pSMART LCAmp
CAG promoter
pEASY-T1 Simple (linearized)
pJET1.2 blunt
pSMART LCAmp (linearized)
chicken beta-actin promoter
pEASY-T3 (linearized)
pJW168
pSMART LCKan
CLIP-tag
pEASY-T5 Zero (linearized)
pKF 18k-2
pSMART LCKan (linearized)
CMV promoter
pEX-1
pKF 19k-2
pSP64 Poly(A)
GAL1 promoter
pEX-A
pKOV
pSP72
GST
pEX-C-GST
pLATE11 (linearized)
pSP73
HaloTag
pEX-C-His
pLATE31 (linearized)
pSpark Done (linearized)
HSV TK promoter
pEX-K
pLATE51 (linearized)
pSpark I (linearized)
lac operator
pEX-N-GST
pLATE52 (linearized)
pSpark II (linearized)
lac promoter
pEX-N-His
pLEXX-AK
pSpark III (linearized)
lac UV5 promoter
pEX-N-His-GST
pLPS-B (linearized)
pSpark IV (linearized)
lacZ
pEX1
pMAL-c5E
pSpark V (linearized)
lambda
pEX2
pMAL-c5G
pSpot1
LITMUS28
pEX3
pMAL-c5X
pSpot2
LITMUS28i
pEZSeq-Amp
pMAL-c5X-His
pSpot3
LITMUS29
pEZSeq-Amp (linearized)
pMAL-c6T
pSpot4
LITMUS38
pEZSeq-Kan
pMAL-p5E
pSTBlue-1
LITMUS38i
pEZSeq-Kan (linearized)
pMAL-p5G
pSTV28
LITMUS39
pEZ BAC
pMAL-p5X
pSTV29
M13mp18
pEZ BAC (BamHI linearized)
pMCS5
pT7Blue
M13mp19
pEZ BAC (HpaI linearized)
pMiniT
pT7Blue-2
MBP
pF1A T7
pMiniT (linearized)
pT7Blue-3
pACYC177
pF1K T7
pMiniT 2.0
pT7CFE1-CFtag
pACYC184
pF25A ICE T7
pMiniT 2.0 (linearized)
pT7CFE1-CGFP-HA-His
pAN7-1
pF25K ICE T7
pMMB190
pT7CFE1-CGST-HA-His
pANT7_cGST
pF3A WG (BYDV)
pMMB206
pT7CFE1-CHA
pANT7_nHA
pF3K WG (BYDV)
pMMB207
pT7CFE1-CHis
pAUR316
pFC20A HaloTag T7 SP6
pMMB208
pT7CFE1-CMyc
pBC KS(+)
pFC20K HaloTag T7 SP6
pMMB66EH
pT7CFE1-NFtag
pBC KS(-)
pFC30A His6HaloTag T7
pMMB66HE
pT7CFE1-NHA
pBC SK(+)
pFC30K His6HaloTag T7
pMMB67EH
pT7CFE1-NHA-CHis
pBC SK(-)
pFC7A (HQ)
pMMB67HE
pT7CFE1-NHis
pBeloBAC11
pFC7K (HQ)
pNEB193
pT7CFE1-NHis-GST
pBluescript II KS(+)
pFN18A HaloTag T7
pNEB206A (linearized)
pT7CFE1-NHis-GST-CHA
pBluescript II KS(-)
pFN18K HaloTag T7
pOSIP-CH
pT7CFE1-NMyc
pBluescript II SK(+)
pFN19A HaloTag T7 SP6
pOSIP-CO
pTOP Blunt V2 (linearized)
pBluescript II SK(-)
pFN19K HaloTag T7 SP6
pOSIP-CT
pTrcHis A
pBluescript KS(+)
pFN29A His6HaloTag T7
pOSIP-KC
pTrcHis B
pBluescript KS(-)
pFN29K His6HaloTag T7
pOSIP-KH
pTrcHis C
pBluescript SK(+)
pFN2A (GST)
pOSIP-KL
pTrcHis2 A
pBluescript SK(-)
pFN2K (GST)
pOSIP-KO
pTrcHis2 B
pBR322
pFN6A (HQ)
pOSIP-KP
pTrcHis2 C
pBS KS(+)
pFN6K (HQ)
pOSIP-KT
pTV 118N
pBS KS(-)
pGEM-11Zf(+)
pOSIP-TH
pTWV228
pBS SK(+)
pGEM-11Zf(-)
pOSIP-TO
pTXB1
pBS SK(-)
pGEM-3Z
pOSIP-TT
pTYB21
pBS(+)
pGEM-3Zf(+)
pPCR-Script Amp SK(+)
pType IIs
pBS(-)
pGEM-3Zf(-)
pPCR-Script Cam SK(+)
pTZ19R
pBSK(+) Simple-Amp
pGEM-4Z
pPTR I
pTZ19U
pBSK(+) Simple-Kan
pGEM-5Zf(+)
pPTR II
pTZ57R
pcDNA3
pGEM-7Zf(+)
pRACE (linearized)
pUAST
pcDNA3.1(+)
pGEM-7Zf(-)
pRANGER-BTB-1
pUC118
pcDNA3.1(-)
pGEM-9Zf(-)
pRANGER-BTB-2
pUC119
pCold I
pGEM-T
pRANGER-BTB-3
pUC18
pCold II
pGEM-T (linearized)
pRANGER-BTB-5
pUC19
pCold III
pGEM-T Easy
pRHA-109
pUC19L
pCold IV
pGEM-T Easy (linearized)
pRHA-113
pUC57
pCold TF
pGGA
pRHA-67
pUC57-Kan
pCR-XL-2-TOPO
pGGAselect
pRSET A
pUC57-Simple
pCR-XL-2-TOPO (linearized)
PGK promoter
pRSET B
pUCmu
pDD
pGS-21a
pRSET C
pVLT31
pDNR-Dual
pH6HTC His6HaloTag T7
pSB1C3
pVLT33
pDNR-LIB
pH6HTN His6HaloTag T7
pSC101
pVLT35
pE-SUMOpro Amp
pHP45(omega)
pSEAP2-Basic
pYES1L
pE-SUMOpro Kan
pHP45(omega)-Km
pSF-Core
pZErO-2
pE-SUMOpro3 Amp
pHP45(omega)-Tc
pSMART BAC v2.0
SNAP-tag
pE-SUMOpro3 Kan
pHSG298
pSMART BAC v2.0 (BamHI linearized)
SP6 promoter
pE-SUMOstar Amp
pHSG299
pSMART BAC v2.0 (EcoRI linearized)
SV40 promoter
pE-SUMOstar Kan
pHSG396
pSMART BAC v2.0 (HindIII linearized)
T3 promoter
pEASY-Blunt (linearized)
pHSG398
pSMART FOS v2.0 (linearized)
T5 promoter
pEASY-Blunt E1 (linearized)
pHY300PLK
pSMART HCAmp
T7 promoter
pEASY-Blunt E2 (linearized)
pICOz
pSMART HCAmp (linearized)
tac promoter
pEASY-Blunt Simple (linearized)
pInvRecA
pSMART HCKan
trc promoter
pEASY-Blunt Zero (linearized)
pJAZZ-OC
pSMART HCKan (linearized)
phiX174
pEASY-Blunt3 (linearized)
pJAZZ-OK

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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