pEX-C-His

PrecisionShuttle™ bacterial vector for inducible expression of a protein with a cleavable C-terminal 6xHis tag.

Sequence Author: OriGene

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HomePlasmidsBasic Cloning VectorspEX-C-His
PsiI (4500) DraIII (4375) BsaAI (4372) XmnI (3822) ScaI (3703) TatI (3701) BsaI (3284) AhdI (3223) AlwNI (2746) EcoRI (175) T7 promoter lac operator RBS BsaBI * - BseRI - BspDI * - ClaI * (279) AscI (295) BglII (302) HindIII (309) NheI (320) BmtI (324) RsrII (326) MluI (332) TEV Site 6xHis NotI (383) AvaI - BsoBI - PaeR7I - PspXI - XhoI (389) 6xHis PmeI (437) FseI (447) BlpI (477) StyI (499) EcoO109I (504) BtgI (555) SacII (558) PflMI (790) BstAPI (891) BclI * (1222) BstEII (1389) PspOMI (1415) ApaI (1419) HincII - HpaI (1714) BsmBI - Esp3I (1823) KasI (1847) NarI * (1848) SfoI (1849) PluTI (1851) BamHI (2201) pEX-C-His 4583 bp
PsiI  (4500)
1 site		 T T A T A A A A T A T T
DraIII  (4375)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
BsaAI  (4372)
1 site		 Y A C G T R R T G C A Y
XmnI  (3822)
1 site		 G A A N N N N T T C C T T N N N N A A G
ScaI  (3703)
1 site		 A G T A C T T C A T G A
TatI  (3701)
1 site		 W G T A C W W C A T G W
BsaI  (3284)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3223)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2746)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
EcoRI  (175)
1 site		 G A A T T C C T T A A G
BsaBI  (279)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BseRI  (279)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BspDI  (279)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (279)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
AscI  (295)
1 site		 G G C G C G C C C C G C G C G G
BglII  (302)
1 site		 A G A T C T T C T A G A
HindIII  (309)
1 site		 A A G C T T T T C G A A
NheI  (320)
1 site		 G C T A G C C G A T C G
BmtI  (324)
1 site		 G C T A G C C G A T C G
RsrII  (326)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
MluI  (332)
1 site		 A C G C G T T G C G C A
NotI  (383)
1 site		 G C G G C C G C C G C C G G C G
AvaI  (389)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BsoBI  (389)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (389)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (389)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (389)
1 site		 C T C G A G G A G C T C
PmeI  (437)
1 site		 G T T T A A A C C A A A T T T G
FseI  (447)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
BlpI  (477)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
StyI  (499)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
EcoO109I  (504)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
BtgI  (555)
1 site		 C C R Y G G G G Y R C C
Sticky ends from different BtgI sites may not be compatible.
SacII  (558)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
PflMI  (790)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
BstAPI  (891)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
BclI  (1222)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BstEII  (1389)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PspOMI  (1415)
1 site		 G G G C C C C C C G G G
ApaI  (1419)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
HincII  (1714)
1 site		 G T Y R A C C A R Y T G
HpaI  (1714)
1 site		 G T T A A C C A A T T G
BsmBI  (1823)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (1823)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
KasI  (1847)
1 site		 G G C G C C C C G C G G
NarI  (1848)
1 site		 G G C G C C C C G C G G
* Blocked by Dcm methylation.
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (1849)
1 site		 G G C G C C C C G C G G
PluTI  (1851)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BamHI  (2201)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
lacI
858 .. 1940  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
858 .. 1940  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
AmpR
3150 .. 4010  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   3150 .. 3941  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3150 .. 4010  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3942 .. 4010  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3150 .. 4010  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
2391 .. 2979  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2391 .. 2979  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
4142 .. 14  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
4142 .. 14  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
AmpR promoter
4011 .. 4115  =  105 bp
AmpR promoter
4011 .. 4115  =  105 bp
lacI promoter
780 .. 857  =  78 bp
lacI promoter
780 .. 857  =  78 bp
MCS
277 .. 337  =  61 bp
multiple cloning site
MCS
277 .. 337  =  61 bp
multiple cloning site
T7 terminator
488 .. 534  =  47 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
488 .. 534  =  47 bp
transcription terminator for bacteriophage T7 RNA polymerase
TEV Site
338 .. 358  =  21 bp
7 amino acids  =  869.9 Da
Product: tobacco etch virus (TEV) protease recognition and cleavage site
TEV Site
338 .. 358  =  21 bp
7 amino acids  =  869.9 Da
Product: tobacco etch virus (TEV) protease recognition and cleavage site
6xHis
359 .. 376  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
359 .. 376  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
TEV Site
395 .. 415  =  21 bp
7 amino acids  =  869.9 Da
Product: tobacco etch virus (TEV) protease recognition and cleavage site
TEV Site
395 .. 415  =  21 bp
7 amino acids  =  869.9 Da
Product: tobacco etch virus (TEV) protease recognition and cleavage site
6xHis
416 .. 433  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
416 .. 433  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
lac operator
226 .. 250  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
226 .. 250  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
T7 promoter
207 .. 225  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
207 .. 225  =  19 bp
promoter for bacteriophage T7 RNA polymerase
RBS
270 .. 275  =  6 bp
ribosome binding site
RBS
270 .. 275  =  6 bp
ribosome binding site
ORF:  3280 .. 3546  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  791 .. 1108  =  318 bp
ORF:  105 amino acids  =  11.2 kDa
ORF:  1697 .. 1960  =  264 bp
ORF:  87 amino acids  =  8.9 kDa
ORF:  2063 .. 2290  =  228 bp
ORF:  75 amino acids  =  8.5 kDa
ORF:  981 .. 1940  =  960 bp
ORF:  319 amino acids  =  34.1 kDa
ORF:  3150 .. 4010  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  1723 .. 1974  =  252 bp
ORF:  83 amino acids  =  9.1 kDa
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Individual Sequences & Maps

BlueScribe
pEASY-T1 (linearized)
pJET1.2
pSMART LCAmp
CAG promoter
pEASY-T1 Simple (linearized)
pJET1.2 blunt
pSMART LCAmp (linearized)
chicken beta-actin promoter
pEASY-T3 (linearized)
pJW168
pSMART LCKan
CLIP-tag
pEASY-T5 Zero (linearized)
pKF 18k-2
pSMART LCKan (linearized)
CMV promoter
pEX-1
pKF 19k-2
pSP64 Poly(A)
GAL1 promoter
pEX-A
pKOV
pSP72
GST
pEX-C-GST
pLATE11 (linearized)
pSP73
HaloTag
pEX-C-His
pLATE31 (linearized)
pSpark Done (linearized)
HSV TK promoter
pEX-K
pLATE51 (linearized)
pSpark I (linearized)
lac operator
pEX-N-GST
pLATE52 (linearized)
pSpark II (linearized)
lac promoter
pEX-N-His
pLEXX-AK
pSpark III (linearized)
lac UV5 promoter
pEX-N-His-GST
pLPS-B (linearized)
pSpark IV (linearized)
lacZ
pEX1
pMAL-c5E
pSpark V (linearized)
lambda
pEX2
pMAL-c5G
pSpot1
LITMUS28
pEX3
pMAL-c5X
pSpot2
LITMUS28i
pEZSeq-Amp
pMAL-c5X-His
pSpot3
LITMUS29
pEZSeq-Amp (linearized)
pMAL-c6T
pSpot4
LITMUS38
pEZSeq-Kan
pMAL-p5E
pSTBlue-1
LITMUS38i
pEZSeq-Kan (linearized)
pMAL-p5G
pSTV28
LITMUS39
pEZ BAC
pMAL-p5X
pSTV29
M13mp18
pEZ BAC (BamHI linearized)
pMCS5
pT7Blue
M13mp19
pEZ BAC (HpaI linearized)
pMiniT
pT7Blue-2
MBP
pF1A T7
pMiniT (linearized)
pT7Blue-3
pACYC177
pF1K T7
pMiniT 2.0
pT7CFE1-CFtag
pACYC184
pF25A ICE T7
pMiniT 2.0 (linearized)
pT7CFE1-CGFP-HA-His
pAN7-1
pF25K ICE T7
pMMB190
pT7CFE1-CGST-HA-His
pANT7_cGST
pF3A WG (BYDV)
pMMB206
pT7CFE1-CHA
pANT7_nHA
pF3K WG (BYDV)
pMMB207
pT7CFE1-CHis
pAUR316
pFC20A HaloTag T7 SP6
pMMB208
pT7CFE1-CMyc
pBC KS(+)
pFC20K HaloTag T7 SP6
pMMB66EH
pT7CFE1-NFtag
pBC KS(-)
pFC30A His6HaloTag T7
pMMB66HE
pT7CFE1-NHA
pBC SK(+)
pFC30K His6HaloTag T7
pMMB67EH
pT7CFE1-NHA-CHis
pBC SK(-)
pFC7A (HQ)
pMMB67HE
pT7CFE1-NHis
pBeloBAC11
pFC7K (HQ)
pNEB193
pT7CFE1-NHis-GST
pBluescript II KS(+)
pFN18A HaloTag T7
pNEB206A (linearized)
pT7CFE1-NHis-GST-CHA
pBluescript II KS(-)
pFN18K HaloTag T7
pOSIP-CH
pT7CFE1-NMyc
pBluescript II SK(+)
pFN19A HaloTag T7 SP6
pOSIP-CO
pTOP Blunt V2 (linearized)
pBluescript II SK(-)
pFN19K HaloTag T7 SP6
pOSIP-CT
pTrcHis A
pBluescript KS(+)
pFN29A His6HaloTag T7
pOSIP-KC
pTrcHis B
pBluescript KS(-)
pFN29K His6HaloTag T7
pOSIP-KH
pTrcHis C
pBluescript SK(+)
pFN2A (GST)
pOSIP-KL
pTrcHis2 A
pBluescript SK(-)
pFN2K (GST)
pOSIP-KO
pTrcHis2 B
pBR322
pFN6A (HQ)
pOSIP-KP
pTrcHis2 C
pBS KS(+)
pFN6K (HQ)
pOSIP-KT
pTV 118N
pBS KS(-)
pGEM-11Zf(+)
pOSIP-TH
pTWV228
pBS SK(+)
pGEM-11Zf(-)
pOSIP-TO
pTXB1
pBS SK(-)
pGEM-3Z
pOSIP-TT
pTYB21
pBS(+)
pGEM-3Zf(+)
pPCR-Script Amp SK(+)
pType IIs
pBS(-)
pGEM-3Zf(-)
pPCR-Script Cam SK(+)
pTZ19R
pBSK(+) Simple-Amp
pGEM-4Z
pPTR I
pTZ19U
pBSK(+) Simple-Kan
pGEM-5Zf(+)
pPTR II
pTZ57R
pcDNA3
pGEM-7Zf(+)
pRACE (linearized)
pUAST
pcDNA3.1(+)
pGEM-7Zf(-)
pRANGER-BTB-1
pUC118
pcDNA3.1(-)
pGEM-9Zf(-)
pRANGER-BTB-2
pUC119
pCold I
pGEM-T
pRANGER-BTB-3
pUC18
pCold II
pGEM-T (linearized)
pRANGER-BTB-5
pUC19
pCold III
pGEM-T Easy
pRHA-109
pUC19L
pCold IV
pGEM-T Easy (linearized)
pRHA-113
pUC57
pCold TF
pGGA
pRHA-67
pUC57-Kan
pCR-XL-2-TOPO
pGGAselect
pRSET A
pUC57-Simple
pCR-XL-2-TOPO (linearized)
PGK promoter
pRSET B
pUCmu
pDD
pGS-21a
pRSET C
pVLT31
pDNR-Dual
pH6HTC His6HaloTag T7
pSB1C3
pVLT33
pDNR-LIB
pH6HTN His6HaloTag T7
pSC101
pVLT35
pE-SUMOpro Amp
pHP45(omega)
pSEAP2-Basic
pYES1L
pE-SUMOpro Kan
pHP45(omega)-Km
pSF-Core
pZErO-2
pE-SUMOpro3 Amp
pHP45(omega)-Tc
pSMART BAC v2.0
SNAP-tag
pE-SUMOpro3 Kan
pHSG298
pSMART BAC v2.0 (BamHI linearized)
SP6 promoter
pE-SUMOstar Amp
pHSG299
pSMART BAC v2.0 (EcoRI linearized)
SV40 promoter
pE-SUMOstar Kan
pHSG396
pSMART BAC v2.0 (HindIII linearized)
T3 promoter
pEASY-Blunt (linearized)
pHSG398
pSMART FOS v2.0 (linearized)
T5 promoter
pEASY-Blunt E1 (linearized)
pHY300PLK
pSMART HCAmp
T7 promoter
pEASY-Blunt E2 (linearized)
pICOz
pSMART HCAmp (linearized)
tac promoter
pEASY-Blunt Simple (linearized)
pInvRecA
pSMART HCKan
trc promoter
pEASY-Blunt Zero (linearized)
pJAZZ-OC
pSMART HCKan (linearized)
phiX174
pEASY-Blunt3 (linearized)
pJAZZ-OK

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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