pVLT33

Broad-host-range bacterial cloning vector with the pUC18/19 MCS and a kanamycin resistance gene.
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HomePlasmidsBasic Cloning VectorspVLT33
BamHI (9702) KpnI (9697) Acc65I (9693) SacI (9691) Eco53kI (9689) EcoRI (9681) lac operator MauBI (9406) AclI (9289) MluI (8949) ApaI (8746) PspOMI (8742) HpaI (8447) BsmBI - Esp3I (8334) FspAI (7670) EcoNI (7520) SgrAI (5432) PasI (4953) XbaI (9708) SbfI (9724) DraIII (414) BglII (764) EagI (834) DrdI (955) PflFI - Tth111I (1046) RsrII (1444) BstBI (1610) NruI (1783) Bsu36I (1804) SfiI (2572) BstZ17I (3947) pVLT33 9762 bp
BamHI  (9702)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
KpnI  (9697)
1 site		 G G T A C C C C A T G G
Acc65I  (9693)
1 site		 G G T A C C C C A T G G
SacI  (9691)
1 site		 G A G C T C C T C G A G
Eco53kI  (9689)
1 site		 G A G C T C C T C G A G
EcoRI  (9681)
1 site		 G A A T T C C T T A A G
MauBI  (9406)
1 site		 C G C G C G C G G C G C G C G C
AclI  (9289)
1 site		 A A C G T T T T G C A A
MluI  (8949)
1 site		 A C G C G T T G C G C A
ApaI  (8746)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
PspOMI  (8742)
1 site		 G G G C C C C C C G G G
HpaI  (8447)
1 site		 G T T A A C C A A T T G
BsmBI  (8334)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (8334)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
FspAI  (7670)
1 site		 R T G C G C A Y Y A C G C G T R
EcoNI  (7520)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
SgrAI  (5432)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
PasI  (4953)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
XbaI  (9708)
1 site		 T C T A G A A G A T C T
SbfI  (9724)
1 site		 C C T G C A G G G G A C G T C C
DraIII  (414)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
BglII  (764)
1 site		 A G A T C T T C T A G A
EagI  (834)
1 site		 C G G C C G G C C G G C
DrdI  (955)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
PflFI  (1046)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1046)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
RsrII  (1444)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BstBI  (1610)
1 site		 T T C G A A A A G C T T
NruI  (1783)
1 site		 T C G C G A A G C G C T
Bsu36I  (1804)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
SfiI  (2572)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BstZ17I  (3947)
1 site		 G T A T A C C A T A T G
lacI
8222 .. 9304  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
8222 .. 9304  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
RSF1010 RepB
4786 .. 5757  =  972 bp
323 amino acids  =  35.9 kDa
Product: replication protein B of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepB
4786 .. 5757  =  972 bp
323 amino acids  =  35.9 kDa
Product: replication protein B of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepC
7094 .. 7945  =  852 bp
283 amino acids  =  31.1 kDa
Product: replication protein C of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepC
7094 .. 7945  =  852 bp
283 amino acids  =  31.1 kDa
Product: replication protein C of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
NeoR/KanR
800 .. 1594  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
800 .. 1594  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
RSF1010 oriV
2724 .. 3118  =  395 bp
replication origin of the broad-host-range plasmid RSF1010; requires the RSF1010 RepA/B/C proteins for replication (Scholz et al., 1989)
RSF1010 oriV
2724 .. 3118  =  395 bp
replication origin of the broad-host-range plasmid RSF1010; requires the RSF1010 RepA/B/C proteins for replication (Scholz et al., 1989)
T4 terminators
1 .. 116  =  116 bp
T4 transcription termination signals
T4 terminators
1 .. 116  =  116 bp
T4 transcription termination signals
T4 terminators
2090 .. 2205  =  116 bp
T4 transcription termination signals
T4 terminators
2090 .. 2205  =  116 bp
T4 transcription termination signals
RSF1010 oriT
3460 .. 3547  =  88 bp
origin of transfer of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 oriT
3460 .. 3547  =  88 bp
origin of transfer of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
lacIq promoter
9305 .. 9382  =  78 bp
In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold.
lacIq promoter
9305 .. 9382  =  78 bp
In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold.
MCS
9681 .. 9737  =  57 bp
pUC18/19 multiple cloning site
MCS
9681 .. 9737  =  57 bp
pUC18/19 multiple cloning site
tac promoter
9612 .. 9640  =  29 bp
3 segments
   Segment 1:  -35  
   9612 .. 9617  =  6 bp
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
tac promoter
9612 .. 9640  =  29 bp
3 segments
   Segment 2:  
   9618 .. 9633  =  16 bp
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
tac promoter
9612 .. 9640  =  29 bp
3 segments
   Segment 3:  -10  
   9634 .. 9640  =  7 bp
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
tac promoter
9612 .. 9640  =  29 bp
3 segments
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
lac operator
9648 .. 9664  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
9648 .. 9664  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
RSF1010 RepA
6268 .. 7107  =  840 bp
279 amino acids  =  29.9 kDa
Product: replication protein A of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepA
6268 .. 7107  =  840 bp
279 amino acids  =  29.9 kDa
Product: replication protein A of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
ORF:  1615 .. 2037  =  423 bp
ORF:  140 amino acids  =  15.5 kDa
ORF:  3628 .. 5757  =  2130 bp
ORF:  709 amino acids  =  78.0 kDa
ORF:  6268 .. 7107  =  840 bp
ORF:  279 amino acids  =  29.9 kDa
ORF:  800 .. 1594  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  2924 .. 3352  =  429 bp
ORF:  142 amino acids  =  15.3 kDa
ORF:  3470 .. 3877  =  408 bp
ORF:  135 amino acids  =  14.3 kDa
ORF:  4376 .. 4789  =  414 bp
ORF:  137 amino acids  =  15.1 kDa
ORF:  5213 .. 5857  =  645 bp
ORF:  214 amino acids  =  22.8 kDa
ORF:  6995 .. 7945  =  951 bp
ORF:  316 amino acids  =  34.4 kDa
ORF:  972 .. 1358  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  2310 .. 2717  =  408 bp
ORF:  135 amino acids  =  15.0 kDa
ORF:  1645 .. 1890  =  246 bp
ORF:  81 amino acids  =  8.4 kDa
ORF:  3145 .. 3429  =  285 bp
ORF:  94 amino acids  =  10.9 kDa
ORF:  3499 .. 4155  =  657 bp
ORF:  218 amino acids  =  22.9 kDa
ORF:  4348 .. 4686  =  339 bp
ORF:  112 amino acids  =  12.0 kDa
ORF:  4903 .. 5496  =  594 bp
ORF:  197 amino acids  =  20.5 kDa
ORF:  6265 .. 7032  =  768 bp
ORF:  255 amino acids  =  27.0 kDa
ORF:  7435 .. 7776  =  342 bp
ORF:  113 amino acids  =  12.6 kDa
ORF:  2346 .. 2591  =  246 bp
ORF:  81 amino acids  =  9.4 kDa
ORF:  3795 .. 4025  =  231 bp
ORF:  76 amino acids  =  8.4 kDa
ORF:  4692 .. 5045  =  354 bp
ORF:  117 amino acids  =  12.6 kDa
ORF:  6519 .. 6884  =  366 bp
ORF:  121 amino acids  =  13.3 kDa
ORF:  8202 .. 8465  =  264 bp
ORF:  87 amino acids  =  8.9 kDa
ORF:  9054 .. 9371  =  318 bp
ORF:  105 amino acids  =  11.2 kDa
ORF:  1109 .. 1645  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
ORF:  2354 .. 2584  =  231 bp
ORF:  76 amino acids  =  8.5 kDa
ORF:  7328 .. 7666  =  339 bp
ORF:  112 amino acids  =  12.1 kDa
ORF:  8222 .. 9181  =  960 bp
ORF:  319 amino acids  =  34.1 kDa
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Individual Sequences & Maps

BlueScribe
pEASY-T1 (linearized)
pJET1.2
pSMART LCAmp
CAG promoter
pEASY-T1 Simple (linearized)
pJET1.2 blunt
pSMART LCAmp (linearized)
chicken beta-actin promoter
pEASY-T3 (linearized)
pJW168
pSMART LCKan
CLIP-tag
pEASY-T5 Zero (linearized)
pKF 18k-2
pSMART LCKan (linearized)
CMV promoter
pEX-1
pKF 19k-2
pSP64 Poly(A)
GAL1 promoter
pEX-A
pKOV
pSP72
GST
pEX-C-GST
pLATE11 (linearized)
pSP73
HaloTag
pEX-C-His
pLATE31 (linearized)
pSpark Done (linearized)
HSV TK promoter
pEX-K
pLATE51 (linearized)
pSpark I (linearized)
lac operator
pEX-N-GST
pLATE52 (linearized)
pSpark II (linearized)
lac promoter
pEX-N-His
pLEXX-AK
pSpark III (linearized)
lac UV5 promoter
pEX-N-His-GST
pLPS-B (linearized)
pSpark IV (linearized)
lacZ
pEX1
pMAL-c5E
pSpark V (linearized)
lambda
pEX2
pMAL-c5G
pSpot1
LITMUS28
pEX3
pMAL-c5X
pSpot2
LITMUS28i
pEZSeq-Amp
pMAL-c5X-His
pSpot3
LITMUS29
pEZSeq-Amp (linearized)
pMAL-c6T
pSpot4
LITMUS38
pEZSeq-Kan
pMAL-p5E
pSTBlue-1
LITMUS38i
pEZSeq-Kan (linearized)
pMAL-p5G
pSTV28
LITMUS39
pEZ BAC
pMAL-p5X
pSTV29
M13mp18
pEZ BAC (BamHI linearized)
pMCS5
pT7Blue
M13mp19
pEZ BAC (HpaI linearized)
pMiniT
pT7Blue-2
MBP
pF1A T7
pMiniT (linearized)
pT7Blue-3
pACYC177
pF1K T7
pMiniT 2.0
pT7CFE1-CFtag
pACYC184
pF25A ICE T7
pMiniT 2.0 (linearized)
pT7CFE1-CGFP-HA-His
pAN7-1
pF25K ICE T7
pMMB190
pT7CFE1-CGST-HA-His
pANT7_cGST
pF3A WG (BYDV)
pMMB206
pT7CFE1-CHA
pANT7_nHA
pF3K WG (BYDV)
pMMB207
pT7CFE1-CHis
pAUR316
pFC20A HaloTag T7 SP6
pMMB208
pT7CFE1-CMyc
pBC KS(+)
pFC20K HaloTag T7 SP6
pMMB66EH
pT7CFE1-NFtag
pBC KS(-)
pFC30A His6HaloTag T7
pMMB66HE
pT7CFE1-NHA
pBC SK(+)
pFC30K His6HaloTag T7
pMMB67EH
pT7CFE1-NHA-CHis
pBC SK(-)
pFC7A (HQ)
pMMB67HE
pT7CFE1-NHis
pBeloBAC11
pFC7K (HQ)
pNEB193
pT7CFE1-NHis-GST
pBluescript II KS(+)
pFN18A HaloTag T7
pNEB206A (linearized)
pT7CFE1-NHis-GST-CHA
pBluescript II KS(-)
pFN18K HaloTag T7
pOSIP-CH
pT7CFE1-NMyc
pBluescript II SK(+)
pFN19A HaloTag T7 SP6
pOSIP-CO
pTOP Blunt V2 (linearized)
pBluescript II SK(-)
pFN19K HaloTag T7 SP6
pOSIP-CT
pTrcHis A
pBluescript KS(+)
pFN29A His6HaloTag T7
pOSIP-KC
pTrcHis B
pBluescript KS(-)
pFN29K His6HaloTag T7
pOSIP-KH
pTrcHis C
pBluescript SK(+)
pFN2A (GST)
pOSIP-KL
pTrcHis2 A
pBluescript SK(-)
pFN2K (GST)
pOSIP-KO
pTrcHis2 B
pBR322
pFN6A (HQ)
pOSIP-KP
pTrcHis2 C
pBS KS(+)
pFN6K (HQ)
pOSIP-KT
pTV 118N
pBS KS(-)
pGEM-11Zf(+)
pOSIP-TH
pTWV228
pBS SK(+)
pGEM-11Zf(-)
pOSIP-TO
pTXB1
pBS SK(-)
pGEM-3Z
pOSIP-TT
pTYB21
pBS(+)
pGEM-3Zf(+)
pPCR-Script Amp SK(+)
pType IIs
pBS(-)
pGEM-3Zf(-)
pPCR-Script Cam SK(+)
pTZ19R
pBSK(+) Simple-Amp
pGEM-4Z
pPTR I
pTZ19U
pBSK(+) Simple-Kan
pGEM-5Zf(+)
pPTR II
pTZ57R
pcDNA3
pGEM-7Zf(+)
pRACE (linearized)
pUAST
pcDNA3.1(+)
pGEM-7Zf(-)
pRANGER-BTB-1
pUC118
pcDNA3.1(-)
pGEM-9Zf(-)
pRANGER-BTB-2
pUC119
pCold I
pGEM-T
pRANGER-BTB-3
pUC18
pCold II
pGEM-T (linearized)
pRANGER-BTB-5
pUC19
pCold III
pGEM-T Easy
pRHA-109
pUC19L
pCold IV
pGEM-T Easy (linearized)
pRHA-113
pUC57
pCold TF
pGGA
pRHA-67
pUC57-Kan
pCR-XL-2-TOPO
pGGAselect
pRSET A
pUC57-Simple
pCR-XL-2-TOPO (linearized)
PGK promoter
pRSET B
pUCmu
pDD
pGS-21a
pRSET C
pVLT31
pDNR-Dual
pH6HTC His6HaloTag T7
pSB1C3
pVLT33
pDNR-LIB
pH6HTN His6HaloTag T7
pSC101
pVLT35
pE-SUMOpro Amp
pHP45(omega)
pSEAP2-Basic
pYES1L
pE-SUMOpro Kan
pHP45(omega)-Km
pSF-Core
pZErO-2
pE-SUMOpro3 Amp
pHP45(omega)-Tc
pSMART BAC v2.0
SNAP-tag
pE-SUMOpro3 Kan
pHSG298
pSMART BAC v2.0 (BamHI linearized)
SP6 promoter
pE-SUMOstar Amp
pHSG299
pSMART BAC v2.0 (EcoRI linearized)
SV40 promoter
pE-SUMOstar Kan
pHSG396
pSMART BAC v2.0 (HindIII linearized)
T3 promoter
pEASY-Blunt (linearized)
pHSG398
pSMART FOS v2.0 (linearized)
T5 promoter
pEASY-Blunt E1 (linearized)
pHY300PLK
pSMART HCAmp
T7 promoter
pEASY-Blunt E2 (linearized)
pICOz
pSMART HCAmp (linearized)
tac promoter
pEASY-Blunt Simple (linearized)
pInvRecA
pSMART HCKan
trc promoter
pEASY-Blunt Zero (linearized)
pJAZZ-OC
pSMART HCKan (linearized)
phiX174
pEASY-Blunt3 (linearized)
pJAZZ-OK

Plasmid Sets

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TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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