pJAZZ-OC

Linear bacterial vector with a chloramphenicol resistance gene for cloning DNA sequences that are unstable in circular plasmids.

Sequence Author: Lucigen

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HomePlasmidsBasic Cloning VectorspJAZZ-OC
12,500 10,000 7500 5000 2500 End (12,887) PacI (12,391) AanI (12,044) BglII (11,781) tonB terminator AvrII (11,729) NcoI (11,583) cat promoter rrnB T1 terminator NZ RevC (10,877 .. 10,898) SwaI (10,898) NotI (10,845) AhdI (10,836) MCS-R SmaI (10,821) PvuI (10,769) BsrBI (10,494) lac promoter BmtI (10,366) NheI (10,362) NaeI (10,355) NgoMIV (10,353) SmaI (10,333) ApaI (10,324) PspOMI (10,320) MCS-L AhdI (10,312) NotI (10,300) SpeI (10,293) SL1 (10,272 .. 10,292) T7Te terminator XbaI (10,207) AanI (9985) AanI (9948) BssSI (9891) RsrII (9656) BbvCI (8044) AanI (7664) BbeI (7282) SfoI (7280) NarI (7279) KasI (7278) XhoI PspXI PaeR7I (6350) BsrGI (5170) AanI (4980) AatII (4824) ZraI (4822) BstBI (4086) SexAI * (3996) MauBI (3125) SacI (1874) Eco53kI (1872) AarI (1785) PpuMI (1632) SgrDI (1402) AanI (1328) BstZ17I (1064) PmeI (956) AanI (728) Start (0) telL TelN RepA CB lacZ α CmR telR N15 ori pJAZZ®-OC 12,887 bp
End  (12,887)
0 sites
PacI  (12,391)
1 site		 T T A A T T A A A A T T A A T T
AanI  (12,044)
7 sites		 T T A T A A A A T A T T
BglII  (11,781)
1 site		 A G A T C T T C T A G A
AvrII  (11,729)
1 site		 C C T A G G G G A T C C
NcoI  (11,583)
1 site		 C C A T G G G G T A C C
SwaI  (10,898)
1 site		 A T T T A A A T T A A A T T T A
SwaI is typically used at 25°C, but is 50% active at 37°C.
NotI  (10,845)
2 sites		 G C G G C C G C C G C C G G C G
AhdI  (10,836)
2 sites		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SmaI  (10,821)
2 sites		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
PvuI  (10,769)
1 site		 C G A T C G G C T A G C
BsrBI  (10,494)
1 site		 C C G C T C G G C G A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
BmtI  (10,366)
1 site		 G C T A G C C G A T C G
NheI  (10,362)
1 site		 G C T A G C C G A T C G
NaeI  (10,355)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (10,353)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
SmaI  (10,333)
2 sites		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
ApaI  (10,324)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
PspOMI  (10,320)
1 site		 G G G C C C C C C G G G
AhdI  (10,312)
2 sites		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
NotI  (10,300)
2 sites		 G C G G C C G C C G C C G G C G
SpeI  (10,293)
1 site		 A C T A G T T G A T C A
XbaI  (10,207)
1 site		 T C T A G A A G A T C T
AanI  (9985)
7 sites		 T T A T A A A A T A T T
AanI  (9948)
7 sites		 T T A T A A A A T A T T
BssSI  (9891)
1 site		 C A C G A G G T G C T C
RsrII  (9656)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BbvCI  (8044)
1 site		 C C T C A G C G G A G T C G
AanI  (7664)
7 sites		 T T A T A A A A T A T T
BbeI  (7282)
1 site		 G G C G C C C C G C G G
Cleavage may be enhanced when more than one copy of the BbeI recognition sequence is present.
SfoI  (7280)
1 site		 G G C G C C C C G C G G
NarI  (7279)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (7278)
1 site		 G G C G C C C C G C G G
XhoI  (6350)
1 site		 C T C G A G G A G C T C
PspXI  (6350)
1 site		 V C T C G A G B B G A G C T C V
PaeR7I  (6350)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
BsrGI  (5170)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
AanI  (4980)
7 sites		 T T A T A A A A T A T T
AatII  (4824)
1 site		 G A C G T C C T G C A G
ZraI  (4822)
1 site		 G A C G T C C T G C A G
BstBI  (4086)
1 site		 T T C G A A A A G C T T
SexAI  (3996)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
MauBI  (3125)
1 site		 C G C G C G C G G C G C G C G C
SacI  (1874)
1 site		 G A G C T C C T C G A G
Eco53kI  (1872)
1 site		 G A G C T C C T C G A G
AarI  (1785)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Cleavage may be enhanced when more than one copy of the AarI recognition sequence is present.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
PpuMI  (1632)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
SgrDI  (1402)
1 site		 C G T C G A C G G C A G C T G C
AanI  (1328)
7 sites		 T T A T A A A A T A T T
BstZ17I  (1064)
1 site		 G T A T A C C A T A T G
PmeI  (956)
1 site		 G T T T A A A C C A A A T T T G
AanI  (728)
7 sites		 T T A T A A A A T A T T
Start  (0)
0 sites
NZ RevC
22-mer  /  32% GC
1 binding site
10,877 .. 10,898  =  22 annealed bases
Tm  =  52°C
sequencing primer
SL1
21-mer  /  57% GC
1 binding site
10,272 .. 10,292  =  21 annealed bases
Tm  =  59°C
sequencing primer
telL
1 .. 28  =  28 bp
telomeric sequence from coliphage N15
telL
1 .. 28  =  28 bp
telomeric sequence from coliphage N15
TelN
193 .. 2088  =  1896 bp
631 amino acids  =  72.3 kDa
Product: protelomerase of coliphage N15
generates the covalently closed hairpin telomeres
TelN
193 .. 2088  =  1896 bp
631 amino acids  =  72.3 kDa
Product: protelomerase of coliphage N15
generates the covalently closed hairpin telomeres
RepA
5084 .. 9058  =  3975 bp
1324 amino acids  =  149.5 kDa
Product: replication protein of coliphage N15
RepA
5084 .. 9058  =  3975 bp
1324 amino acids  =  149.5 kDa
Product: replication protein of coliphage N15
CB
9307 .. 9915  =  609 bp
202 amino acids  =  22.7 kDa
Product: repressor protein of coliphage N15
CB
9307 .. 9915  =  609 bp
202 amino acids  =  22.7 kDa
Product: repressor protein of coliphage N15
T7Te terminator
10,213 .. 10,240  =  28 bp
phage T7 early transcription terminator
T7Te terminator
10,213 .. 10,240  =  28 bp
phage T7 early transcription terminator
MCS-L
10,299 .. 10,336  =  38 bp
multiple cloning site, left side
MCS-L
10,299 .. 10,336  =  38 bp
multiple cloning site, left side
lac promoter
10,450 .. 10,480  =  31 bp
3 segments
   Segment 1:  -35  
   10,450 .. 10,455  =  6 bp
promoter for the E. coli lac operon
lac promoter
10,450 .. 10,480  =  31 bp
3 segments
   Segment 2:  
   10,456 .. 10,473  =  18 bp
promoter for the E. coli lac operon
lac promoter
10,450 .. 10,480  =  31 bp
3 segments
   Segment 3:  -10  
   10,474 .. 10,480  =  7 bp
promoter for the E. coli lac operon
lac promoter
10,450 .. 10,480  =  31 bp
3 segments
promoter for the E. coli lac operon
lacZα
10,524 .. 10,796  =  273 bp
90 amino acids  =  10.2 kDa
Product: LacZα fragment of β-galactosidase
allows blue/white screening
lacZα
10,524 .. 10,796  =  273 bp
90 amino acids  =  10.2 kDa
Product: LacZα fragment of β-galactosidase
allows blue/white screening
MCS-R
10,819 .. 10,851  =  33 bp
multiple cloning site, right side
MCS-R
10,819 .. 10,851  =  33 bp
multiple cloning site, right side
rrnB T1 terminator
10,917 .. 10,962  =  46 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
10,917 .. 10,962  =  46 bp
transcription terminator T1 from the E. coli rrnB gene
cat promoter
10,966 .. 11,068  =  103 bp
promoter of the E. coli cat gene
cat promoter
10,966 .. 11,068  =  103 bp
promoter of the E. coli cat gene
CmR
11,069 .. 11,728  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
11,069 .. 11,728  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
tonB terminator
11,742 .. 11,773  =  32 bp
bidirectional E. coli tonB-P14 transcription terminator
tonB terminator
11,742 .. 11,773  =  32 bp
bidirectional E. coli tonB-P14 transcription terminator
telR
12,860 .. 12,887  =  28 bp
telomeric sequence from coliphage N15
telR
12,860 .. 12,887  =  28 bp
telomeric sequence from coliphage N15
N15 ori
5335 .. 5424  =  90 bp
bidirectional replication origin of coliphage N15
N15 ori
5335 .. 5424  =  90 bp
bidirectional replication origin of coliphage N15
ORF:  193 .. 2088  =  1896 bp
ORF:  631 amino acids  =  72.3 kDa
ORF:  9997 .. 10,320  =  324 bp
ORF:  107 amino acids  =  11.9 kDa
ORF:  10,579 .. 10,878  =  300 bp
ORF:  99 amino acids  =  10.8 kDa
ORF:  1313 .. 1651  =  339 bp
ORF:  112 amino acids  =  12.5 kDa
ORF:  3032 .. 3268  =  237 bp
ORF:  78 amino acids  =  9.0 kDa
ORF:  7082 .. 7555  =  474 bp
ORF:  157 amino acids  =  17.8 kDa
ORF:  11,069 .. 11,728  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  2067 .. 2291  =  225 bp
ORF:  74 amino acids  =  8.5 kDa
ORF:  5169 .. 5426  =  258 bp
ORF:  85 amino acids  =  8.7 kDa
ORF:  5466 .. 5786  =  321 bp
ORF:  106 amino acids  =  11.7 kDa
ORF:  6684 .. 6917  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
ORF:  7317 .. 7544  =  228 bp
ORF:  75 amino acids  =  8.2 kDa
ORF:  8127 .. 8402  =  276 bp
ORF:  91 amino acids  =  10.0 kDa
ORF:  10,524 .. 10,796  =  273 bp
ORF:  90 amino acids  =  10.2 kDa
ORF:  1464 .. 1817  =  354 bp
ORF:  117 amino acids  =  12.3 kDa
ORF:  2262 .. 3056  =  795 bp
ORF:  264 amino acids  =  29.7 kDa
ORF:  4824 .. 5072  =  249 bp
ORF:  82 amino acids  =  9.3 kDa
ORF:  3053 .. 3283  =  231 bp
ORF:  76 amino acids  =  8.4 kDa
ORF:  3812 .. 4252  =  441 bp
ORF:  146 amino acids  =  17.5 kDa
ORF:  5084 .. 9058  =  3975 bp
ORF:  1324 amino acids  =  149.5 kDa
ORF:  10,643 .. 10,876  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
ORF:  11,744 .. 12,445  =  702 bp
ORF:  233 amino acids  =  26.0 kDa
ORF:  1300 .. 1605  =  306 bp
ORF:  101 amino acids  =  11.1 kDa
ORF:  1639 .. 1926  =  288 bp
ORF:  95 amino acids  =  10.9 kDa
ORF:  3280 .. 3582  =  303 bp
ORF:  100 amino acids  =  10.9 kDa
ORF:  4165 .. 4473  =  309 bp
ORF:  102 amino acids  =  11.7 kDa
ORF:  4498 .. 4827  =  330 bp
ORF:  109 amino acids  =  12.6 kDa
ORF:  6439 .. 6696  =  258 bp
ORF:  85 amino acids  =  9.2 kDa
ORF:  9307 .. 9915  =  609 bp
ORF:  202 amino acids  =  22.7 kDa
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Individual Sequences & Maps

BlueScribe
pEASY-T1 (linearized)
pJET1.2
pSMART LCAmp
CAG promoter
pEASY-T1 Simple (linearized)
pJET1.2 blunt
pSMART LCAmp (linearized)
chicken beta-actin promoter
pEASY-T3 (linearized)
pJW168
pSMART LCKan
CLIP-tag
pEASY-T5 Zero (linearized)
pKF 18k-2
pSMART LCKan (linearized)
CMV promoter
pEX-1
pKF 19k-2
pSP64 Poly(A)
GAL1 promoter
pEX-A
pKOV
pSP72
GST
pEX-C-GST
pLATE11 (linearized)
pSP73
HaloTag
pEX-C-His
pLATE31 (linearized)
pSpark Done (linearized)
HSV TK promoter
pEX-K
pLATE51 (linearized)
pSpark I (linearized)
lac operator
pEX-N-GST
pLATE52 (linearized)
pSpark II (linearized)
lac promoter
pEX-N-His
pLEXX-AK
pSpark III (linearized)
lac UV5 promoter
pEX-N-His-GST
pLPS-B (linearized)
pSpark IV (linearized)
lacZ
pEX1
pMAL-c5E
pSpark V (linearized)
lambda
pEX2
pMAL-c5G
pSpot1
LITMUS28
pEX3
pMAL-c5X
pSpot2
LITMUS28i
pEZSeq-Amp
pMAL-c5X-His
pSpot3
LITMUS29
pEZSeq-Amp (linearized)
pMAL-c6T
pSpot4
LITMUS38
pEZSeq-Kan
pMAL-p5E
pSTBlue-1
LITMUS38i
pEZSeq-Kan (linearized)
pMAL-p5G
pSTV28
LITMUS39
pEZ BAC
pMAL-p5X
pSTV29
M13mp18
pEZ BAC (BamHI linearized)
pMCS5
pT7Blue
M13mp19
pEZ BAC (HpaI linearized)
pMiniT
pT7Blue-2
MBP
pF1A T7
pMiniT (linearized)
pT7Blue-3
pACYC177
pF1K T7
pMiniT 2.0
pT7CFE1-CFtag
pACYC184
pF25A ICE T7
pMiniT 2.0 (linearized)
pT7CFE1-CGFP-HA-His
pAN7-1
pF25K ICE T7
pMMB190
pT7CFE1-CGST-HA-His
pANT7_cGST
pF3A WG (BYDV)
pMMB206
pT7CFE1-CHA
pANT7_nHA
pF3K WG (BYDV)
pMMB207
pT7CFE1-CHis
pAUR316
pFC20A HaloTag T7 SP6
pMMB208
pT7CFE1-CMyc
pBC KS(+)
pFC20K HaloTag T7 SP6
pMMB66EH
pT7CFE1-NFtag
pBC KS(-)
pFC30A His6HaloTag T7
pMMB66HE
pT7CFE1-NHA
pBC SK(+)
pFC30K His6HaloTag T7
pMMB67EH
pT7CFE1-NHA-CHis
pBC SK(-)
pFC7A (HQ)
pMMB67HE
pT7CFE1-NHis
pBeloBAC11
pFC7K (HQ)
pNEB193
pT7CFE1-NHis-GST
pBluescript II KS(+)
pFN18A HaloTag T7
pNEB206A (linearized)
pT7CFE1-NHis-GST-CHA
pBluescript II KS(-)
pFN18K HaloTag T7
pOSIP-CH
pT7CFE1-NMyc
pBluescript II SK(+)
pFN19A HaloTag T7 SP6
pOSIP-CO
pTOP Blunt V2 (linearized)
pBluescript II SK(-)
pFN19K HaloTag T7 SP6
pOSIP-CT
pTrcHis A
pBluescript KS(+)
pFN29A His6HaloTag T7
pOSIP-KC
pTrcHis B
pBluescript KS(-)
pFN29K His6HaloTag T7
pOSIP-KH
pTrcHis C
pBluescript SK(+)
pFN2A (GST)
pOSIP-KL
pTrcHis2 A
pBluescript SK(-)
pFN2K (GST)
pOSIP-KO
pTrcHis2 B
pBR322
pFN6A (HQ)
pOSIP-KP
pTrcHis2 C
pBS KS(+)
pFN6K (HQ)
pOSIP-KT
pTV 118N
pBS KS(-)
pGEM-11Zf(+)
pOSIP-TH
pTWV228
pBS SK(+)
pGEM-11Zf(-)
pOSIP-TO
pTXB1
pBS SK(-)
pGEM-3Z
pOSIP-TT
pTYB21
pBS(+)
pGEM-3Zf(+)
pPCR-Script Amp SK(+)
pType IIs
pBS(-)
pGEM-3Zf(-)
pPCR-Script Cam SK(+)
pTZ19R
pBSK(+) Simple-Amp
pGEM-4Z
pPTR I
pTZ19U
pBSK(+) Simple-Kan
pGEM-5Zf(+)
pPTR II
pTZ57R
pcDNA3
pGEM-7Zf(+)
pRACE (linearized)
pUAST
pcDNA3.1(+)
pGEM-7Zf(-)
pRANGER-BTB-1
pUC118
pcDNA3.1(-)
pGEM-9Zf(-)
pRANGER-BTB-2
pUC119
pCold I
pGEM-T
pRANGER-BTB-3
pUC18
pCold II
pGEM-T (linearized)
pRANGER-BTB-5
pUC19
pCold III
pGEM-T Easy
pRHA-109
pUC19L
pCold IV
pGEM-T Easy (linearized)
pRHA-113
pUC57
pCold TF
pGGA
pRHA-67
pUC57-Kan
pCR-XL-2-TOPO
pGGAselect
pRSET A
pUC57-Simple
pCR-XL-2-TOPO (linearized)
PGK promoter
pRSET B
pUCmu
pDD
pGS-21a
pRSET C
pVLT31
pDNR-Dual
pH6HTC His6HaloTag T7
pSB1C3
pVLT33
pDNR-LIB
pH6HTN His6HaloTag T7
pSC101
pVLT35
pE-SUMOpro Amp
pHP45(omega)
pSEAP2-Basic
pYES1L
pE-SUMOpro Kan
pHP45(omega)-Km
pSF-Core
pZErO-2
pE-SUMOpro3 Amp
pHP45(omega)-Tc
pSMART BAC v2.0
SNAP-tag
pE-SUMOpro3 Kan
pHSG298
pSMART BAC v2.0 (BamHI linearized)
SP6 promoter
pE-SUMOstar Amp
pHSG299
pSMART BAC v2.0 (EcoRI linearized)
SV40 promoter
pE-SUMOstar Kan
pHSG396
pSMART BAC v2.0 (HindIII linearized)
T3 promoter
pEASY-Blunt (linearized)
pHSG398
pSMART FOS v2.0 (linearized)
T5 promoter
pEASY-Blunt E1 (linearized)
pHY300PLK
pSMART HCAmp
T7 promoter
pEASY-Blunt E2 (linearized)
pICOz
pSMART HCAmp (linearized)
tac promoter
pEASY-Blunt Simple (linearized)
pInvRecA
pSMART HCKan
trc promoter
pEASY-Blunt Zero (linearized)
pJAZZ-OC
pSMART HCKan (linearized)
phiX174
pEASY-Blunt3 (linearized)
pJAZZ-OK

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Mammalian Expression Vectors
Structural Genomics Vectors

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