pSMART BAC v2.0 (EcoRI linearized)

EcoRI-linearized and dephosphorylated BAC vector with inducible copy number, suitable for cloning toxic inserts.

Sequence Author: Lucigen

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HomePlasmidsBasic Cloning VectorspSMART BAC v2.0 (EcoRI linearized)
<EcoRI> (7592) PmlI (7588) AhdI (7580) NotI (7568) SL1 (7520 .. 7540) <EcoRI> (0) PmlI (7) AhdI (15) NotI (22) SR4 (58 .. 78) pSMART® BAC v2.0 7592 bp
End  (7592)
0 sites
PmlI  (7588)
2 sites		 C A C G T G G T G C A C
AhdI  (7580)
2 sites		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
NotI  (7568)
2 sites		 G C G G C C G C C G C C G G C G
Start  (0)
0 sites
PmlI  (7)
2 sites		 C A C G T G G T G C A C
AhdI  (15)
2 sites		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
NotI  (22)
2 sites		 G C G G C C G C C G C C G G C G
SL1
21-mer  /  57% GC
1 binding site
7520 .. 7540  =  21 annealed bases
Tm  =  59°C
SR4
21-mer  /  33% GC
1 binding site
58 .. 78  =  21 annealed bases
Tm  =  52°C
sopA
3185 .. 4360  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F plasmid
sopA
3185 .. 4360  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F plasmid
repE
4939 .. 5694  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the bacterial F plasmid
repE
4939 .. 5694  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the bacterial F plasmid
CmR
228 .. 887  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
228 .. 887  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
oriV
6080 .. 6694  =  615 bp
origin of replication for the bacterial F plasmid
oriV
6080 .. 6694  =  615 bp
origin of replication for the bacterial F plasmid
sopC
1668 .. 2141  =  474 bp
centromere-like partitioning region of the bacterial F plasmid
sopC
1668 .. 2141  =  474 bp
centromere-like partitioning region of the bacterial F plasmid
cos
1009 .. 1408  =  400 bp
lambda cos site; allows packaging into phage lambda particles
cos
1009 .. 1408  =  400 bp
lambda cos site; allows packaging into phage lambda particles
incC
4686 .. 4936  =  251 bp
incompatibility region of the bacterial F plasmid
incC
4686 .. 4936  =  251 bp
incompatibility region of the bacterial F plasmid
ori2
5785 .. 6004  =  220 bp
secondary origin of replication for the bacterial F plasmid; also known as oriS
ori2
5785 .. 6004  =  220 bp
secondary origin of replication for the bacterial F plasmid; also known as oriS
cat promoter
125 .. 227  =  103 bp
promoter of the E. coli cat gene
cat promoter
125 .. 227  =  103 bp
promoter of the E. coli cat gene
rrnBT1 terminator
76 .. 121  =  46 bp
transcription terminator T1 from the E. coli rrnB gene
rrnBT1 terminator
76 .. 121  =  46 bp
transcription terminator T1 from the E. coli rrnB gene
loxP
958 .. 991  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
loxP
958 .. 991  =  34 bp
Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT).
tonB terminator
901 .. 932  =  32 bp
bidirectional E. coli tonB-P14 transcription terminator
tonB terminator
901 .. 932  =  32 bp
bidirectional E. coli tonB-P14 transcription terminator
T7Te terminator
7461 .. 7488  =  28 bp
phage T7 early transcription terminator
T7Te terminator
7461 .. 7488  =  28 bp
phage T7 early transcription terminator
T7 promoter
35 .. 53  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
35 .. 53  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SP6 promoter
7547 .. 7565  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
7547 .. 7565  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
sopB
2214 .. 3185  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F plasmid
sopB
2214 .. 3185  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F plasmid
ORF:  2908 .. 3144  =  237 bp
ORF:  78 amino acids  =  8.3 kDa
ORF:  5305 .. 5538  =  234 bp
ORF:  77 amino acids  =  9.1 kDa
ORF:  228 .. 887  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  7212 .. 7556  =  345 bp
ORF:  114 amino acids  =  13.0 kDa
ORF:  4939 .. 5694  =  756 bp
ORF:  251 amino acids  =  29.4 kDa
ORF:  2214 .. 3185  =  972 bp
ORF:  323 amino acids  =  35.4 kDa
ORF:  5145 .. 5390  =  246 bp
ORF:  81 amino acids  =  8.9 kDa
ORF:  5475 .. 5705  =  231 bp
ORF:  76 amino acids  =  8.7 kDa
ORF:  7089 .. 7436  =  348 bp
ORF:  115 amino acids  =  12.9 kDa
ORF:  2435 .. 2833  =  399 bp
ORF:  132 amino acids  =  15.6 kDa
ORF:  3185 .. 4360  =  1176 bp
ORF:  391 amino acids  =  44.1 kDa
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Individual Sequences & Maps

BlueScribe
pEASY-T1 (linearized)
pJET1.2
pSMART LCAmp
CAG promoter
pEASY-T1 Simple (linearized)
pJET1.2 blunt
pSMART LCAmp (linearized)
chicken beta-actin promoter
pEASY-T3 (linearized)
pJW168
pSMART LCKan
CLIP-tag
pEASY-T5 Zero (linearized)
pKF 18k-2
pSMART LCKan (linearized)
CMV promoter
pEX-1
pKF 19k-2
pSP64 Poly(A)
GAL1 promoter
pEX-A
pKOV
pSP72
GST
pEX-C-GST
pLATE11 (linearized)
pSP73
HaloTag
pEX-C-His
pLATE31 (linearized)
pSpark Done (linearized)
HSV TK promoter
pEX-K
pLATE51 (linearized)
pSpark I (linearized)
lac operator
pEX-N-GST
pLATE52 (linearized)
pSpark II (linearized)
lac promoter
pEX-N-His
pLEXX-AK
pSpark III (linearized)
lac UV5 promoter
pEX-N-His-GST
pLPS-B (linearized)
pSpark IV (linearized)
lacZ
pEX1
pMAL-c5E
pSpark V (linearized)
lambda
pEX2
pMAL-c5G
pSpot1
LITMUS28
pEX3
pMAL-c5X
pSpot2
LITMUS28i
pEZSeq-Amp
pMAL-c5X-His
pSpot3
LITMUS29
pEZSeq-Amp (linearized)
pMAL-c6T
pSpot4
LITMUS38
pEZSeq-Kan
pMAL-p5E
pSTBlue-1
LITMUS38i
pEZSeq-Kan (linearized)
pMAL-p5G
pSTV28
LITMUS39
pEZ BAC
pMAL-p5X
pSTV29
M13mp18
pEZ BAC (BamHI linearized)
pMCS5
pT7Blue
M13mp19
pEZ BAC (HpaI linearized)
pMiniT
pT7Blue-2
MBP
pF1A T7
pMiniT (linearized)
pT7Blue-3
pACYC177
pF1K T7
pMiniT 2.0
pT7CFE1-CFtag
pACYC184
pF25A ICE T7
pMiniT 2.0 (linearized)
pT7CFE1-CGFP-HA-His
pAN7-1
pF25K ICE T7
pMMB190
pT7CFE1-CGST-HA-His
pANT7_cGST
pF3A WG (BYDV)
pMMB206
pT7CFE1-CHA
pANT7_nHA
pF3K WG (BYDV)
pMMB207
pT7CFE1-CHis
pAUR316
pFC20A HaloTag T7 SP6
pMMB208
pT7CFE1-CMyc
pBC KS(+)
pFC20K HaloTag T7 SP6
pMMB66EH
pT7CFE1-NFtag
pBC KS(-)
pFC30A His6HaloTag T7
pMMB66HE
pT7CFE1-NHA
pBC SK(+)
pFC30K His6HaloTag T7
pMMB67EH
pT7CFE1-NHA-CHis
pBC SK(-)
pFC7A (HQ)
pMMB67HE
pT7CFE1-NHis
pBeloBAC11
pFC7K (HQ)
pNEB193
pT7CFE1-NHis-GST
pBluescript II KS(+)
pFN18A HaloTag T7
pNEB206A (linearized)
pT7CFE1-NHis-GST-CHA
pBluescript II KS(-)
pFN18K HaloTag T7
pOSIP-CH
pT7CFE1-NMyc
pBluescript II SK(+)
pFN19A HaloTag T7 SP6
pOSIP-CO
pTOP Blunt V2 (linearized)
pBluescript II SK(-)
pFN19K HaloTag T7 SP6
pOSIP-CT
pTrcHis A
pBluescript KS(+)
pFN29A His6HaloTag T7
pOSIP-KC
pTrcHis B
pBluescript KS(-)
pFN29K His6HaloTag T7
pOSIP-KH
pTrcHis C
pBluescript SK(+)
pFN2A (GST)
pOSIP-KL
pTrcHis2 A
pBluescript SK(-)
pFN2K (GST)
pOSIP-KO
pTrcHis2 B
pBR322
pFN6A (HQ)
pOSIP-KP
pTrcHis2 C
pBS KS(+)
pFN6K (HQ)
pOSIP-KT
pTV 118N
pBS KS(-)
pGEM-11Zf(+)
pOSIP-TH
pTWV228
pBS SK(+)
pGEM-11Zf(-)
pOSIP-TO
pTXB1
pBS SK(-)
pGEM-3Z
pOSIP-TT
pTYB21
pBS(+)
pGEM-3Zf(+)
pPCR-Script Amp SK(+)
pType IIs
pBS(-)
pGEM-3Zf(-)
pPCR-Script Cam SK(+)
pTZ19R
pBSK(+) Simple-Amp
pGEM-4Z
pPTR I
pTZ19U
pBSK(+) Simple-Kan
pGEM-5Zf(+)
pPTR II
pTZ57R
pcDNA3
pGEM-7Zf(+)
pRACE (linearized)
pUAST
pcDNA3.1(+)
pGEM-7Zf(-)
pRANGER-BTB-1
pUC118
pcDNA3.1(-)
pGEM-9Zf(-)
pRANGER-BTB-2
pUC119
pCold I
pGEM-T
pRANGER-BTB-3
pUC18
pCold II
pGEM-T (linearized)
pRANGER-BTB-5
pUC19
pCold III
pGEM-T Easy
pRHA-109
pUC19L
pCold IV
pGEM-T Easy (linearized)
pRHA-113
pUC57
pCold TF
pGGA
pRHA-67
pUC57-Kan
pCR-XL-2-TOPO
pGGAselect
pRSET A
pUC57-Simple
pCR-XL-2-TOPO (linearized)
PGK promoter
pRSET B
pUCmu
pDD
pGS-21a
pRSET C
pVLT31
pDNR-Dual
pH6HTC His6HaloTag T7
pSB1C3
pVLT33
pDNR-LIB
pH6HTN His6HaloTag T7
pSC101
pVLT35
pE-SUMOpro Amp
pHP45(omega)
pSEAP2-Basic
pYES1L
pE-SUMOpro Kan
pHP45(omega)-Km
pSF-Core
pZErO-2
pE-SUMOpro3 Amp
pHP45(omega)-Tc
pSMART BAC v2.0
SNAP-tag
pE-SUMOpro3 Kan
pHSG298
pSMART BAC v2.0 (BamHI linearized)
SP6 promoter
pE-SUMOstar Amp
pHSG299
pSMART BAC v2.0 (EcoRI linearized)
SV40 promoter
pE-SUMOstar Kan
pHSG396
pSMART BAC v2.0 (HindIII linearized)
T3 promoter
pEASY-Blunt (linearized)
pHSG398
pSMART FOS v2.0 (linearized)
T5 promoter
pEASY-Blunt E1 (linearized)
pHY300PLK
pSMART HCAmp
T7 promoter
pEASY-Blunt E2 (linearized)
pICOz
pSMART HCAmp (linearized)
tac promoter
pEASY-Blunt Simple (linearized)
pInvRecA
pSMART HCKan
trc promoter
pEASY-Blunt Zero (linearized)
pJAZZ-OC
pSMART HCKan (linearized)
phiX174
pEASY-Blunt3 (linearized)
pJAZZ-OK

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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