pOSIP-TT

Prokaryotic one-step cloning and chromosomal integration vector encoding a tetracycline resistance marker and the phage φ21 integrase.
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HomePlasmidsBasic Cloning VectorspOSIP-TT
EcoRI (1) rrnB T1 terminator rrnB T1 terminator rrnB T1 terminator rrnB T1 terminator PflFI - Tth111I (6566) λ tL3 terminator BmtI (6094) NheI (6090) SnaBI (5099) SalI (5014) ScaI (4481) PmlI (4204) BspDI - ClaI (3582) Acc65I (13) KpnI (17) BamHI (22) BssHII (70) SrfI (225) BglII (469) SpeI (1126) PstI (1144) PaeR7I - XhoI (1152) PacI (1340) PspOMI (1349) ApaI (1353) AvrII (1418) EarI (1442) PshAI (2090) BtsI - BtsαI (2887) CsiI - SexAI * (3200) DraIII (3576) pOSIP-TT 7395 bp
EcoRI  (1)
1 site		 G A A T T C C T T A A G
PflFI  (6566)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (6566)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BmtI  (6094)
1 site		 G C T A G C C G A T C G
NheI  (6090)
1 site		 G C T A G C C G A T C G
SnaBI  (5099)
1 site		 T A C G T A A T G C A T
SalI  (5014)
1 site		 G T C G A C C A G C T G
ScaI  (4481)
1 site		 A G T A C T T C A T G A
PmlI  (4204)
1 site		 C A C G T G G T G C A C
BspDI  (3582)
1 site		 A T C G A T T A G C T A
ClaI  (3582)
1 site		 A T C G A T T A G C T A
Acc65I  (13)
1 site		 G G T A C C C C A T G G
KpnI  (17)
1 site		 G G T A C C C C A T G G
BamHI  (22)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BssHII  (70)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
SrfI  (225)
1 site		 G C C C G G G C C G G G C C C G
BglII  (469)
1 site		 A G A T C T T C T A G A
SpeI  (1126)
1 site		 A C T A G T T G A T C A
PstI  (1144)
1 site		 C T G C A G G A C G T C
PaeR7I  (1152)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1152)
1 site		 C T C G A G G A G C T C
PacI  (1340)
1 site		 T T A A T T A A A A T T A A T T
PspOMI  (1349)
1 site		 G G G C C C C C C G G G
ApaI  (1353)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
AvrII  (1418)
1 site		 C C T A G G G G A T C C
EarI  (1442)
1 site		 C T C T T C N G A G A A G N N N N
Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
PshAI  (2090)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
BtsI  (2887)
1 site		 G C A G T G N N C G T C A C
BtsαI  (2887)
1 site		 G C A G T G N N C G T C A C
Sticky ends from different BtsαI sites may not be compatible.
CsiI  (3200)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (3200)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
DraIII  (3576)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
TcR
3649 .. 4854  =  1206 bp
401 amino acids  =  43.4 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
TcR
3649 .. 4854  =  1206 bp
401 amino acids  =  43.4 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
phage φ21 integrase
2299 .. 3441  =  1143 bp
380 amino acids  =  43.9 kDa
Product: integrase from phage φ21
phage φ21 integrase
2299 .. 3441  =  1143 bp
380 amino acids  =  43.9 kDa
Product: integrase from phage φ21
λ repressor (ts)
1424 .. 2137  =  714 bp
237 amino acids  =  26.2 kDa
Product: temperature-sensitive variant of the phage λ repressor
thermosensitivity is conferred by the A67T mutation
λ repressor (ts)
1424 .. 2137  =  714 bp
237 amino acids  =  26.2 kDa
Product: temperature-sensitive variant of the phage λ repressor
thermosensitivity is conferred by the A67T mutation
ori
477 .. 1065  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
477 .. 1065  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
phage φ21 attP
5590 .. 6089  =  500 bp
attachment site of phage φ21
phage φ21 attP
5590 .. 6089  =  500 bp
attachment site of phage φ21
R6K γ ori
5064 .. 5452  =  389 bp
γ replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication
R6K γ ori
5064 .. 5452  =  389 bp
γ replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication
ccdB
142 .. 447  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA gyrase
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
ccdB
142 .. 447  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA gyrase
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
λ tL3 terminator
6110 .. 6356  =  247 bp
transcription terminator tL3 from phage λ
λ tL3 terminator
6110 .. 6356  =  247 bp
transcription terminator tL3 from phage λ
lambda t0 terminator
4913 .. 5007  =  95 bp
transcription terminator from phage lambda
lambda t0 terminator
4913 .. 5007  =  95 bp
transcription terminator from phage lambda
rrnB T1 terminator
6676 .. 6762  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
6676 .. 6762  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
6857 .. 6943  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
6857 .. 6943  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
7038 .. 7124  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
7038 .. 7124  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
7219 .. 7305  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
7219 .. 7305  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
MCS 1
1 .. 75  =  75 bp
multiple cloning site, part 1
MCS 1
1 .. 75  =  75 bp
multiple cloning site, part 1
tetR/tetA promoters
3587 .. 3641  =  55 bp
overlapping promoters for bacterial tetR and tetA
tetR/tetA promoters
3587 .. 3641  =  55 bp
overlapping promoters for bacterial tetR and tetA
FRT
5463 .. 5510  =  48 bp
FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011).
FRT
5463 .. 5510  =  48 bp
FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011).
FRT (minimal)
1384 .. 1417  =  34 bp
supports FLP-mediated excision but not integration (Turan and Bode, 2011)
FRT (minimal)
1384 .. 1417  =  34 bp
supports FLP-mediated excision but not integration (Turan and Bode, 2011)
MCS 2
1126 .. 1157  =  32 bp
multiple cloning site, part 2
MCS 2
1126 .. 1157  =  32 bp
multiple cloning site, part 2
tonB terminator
1276 .. 1307  =  32 bp
bidirectional E. coli tonB-P14 transcription terminator
tonB terminator
1276 .. 1307  =  32 bp
bidirectional E. coli tonB-P14 transcription terminator
rrnB T2 terminator
1200 .. 1227  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
rrnB T2 terminator
1200 .. 1227  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
tet operator
3622 .. 3640  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
3622 .. 3640  =  19 bp
bacterial operator O2 for the tetR and tetA genes
ORF:  142 .. 447  =  306 bp
ORF:  101 amino acids  =  11.7 kDa
ORF:  2299 .. 3441  =  1143 bp
ORF:  380 amino acids  =  43.9 kDa
ORF:  3649 .. 4854  =  1206 bp
ORF:  401 amino acids  =  43.4 kDa
ORF:  6592 .. 6831  =  240 bp
ORF:  79 amino acids  =  9.2 kDa
ORF:  3058 .. 3294  =  237 bp
ORF:  78 amino acids  =  8.9 kDa
ORF:  3355 .. 3582  =  228 bp
ORF:  75 amino acids  =  8.5 kDa
ORF:  4023 .. 4283  =  261 bp
ORF:  86 amino acids  =  9.3 kDa
ORF:  1424 .. 2137  =  714 bp
ORF:  237 amino acids  =  26.2 kDa
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Individual Sequences & Maps

BlueScribe
pEASY-T1 (linearized)
pJET1.2
pSMART LCAmp
CAG promoter
pEASY-T1 Simple (linearized)
pJET1.2 blunt
pSMART LCAmp (linearized)
chicken beta-actin promoter
pEASY-T3 (linearized)
pJW168
pSMART LCKan
CLIP-tag
pEASY-T5 Zero (linearized)
pKF 18k-2
pSMART LCKan (linearized)
CMV promoter
pEX-1
pKF 19k-2
pSP64 Poly(A)
GAL1 promoter
pEX-A
pKOV
pSP72
GST
pEX-C-GST
pLATE11 (linearized)
pSP73
HaloTag
pEX-C-His
pLATE31 (linearized)
pSpark Done (linearized)
HSV TK promoter
pEX-K
pLATE51 (linearized)
pSpark I (linearized)
lac operator
pEX-N-GST
pLATE52 (linearized)
pSpark II (linearized)
lac promoter
pEX-N-His
pLEXX-AK
pSpark III (linearized)
lac UV5 promoter
pEX-N-His-GST
pLPS-B (linearized)
pSpark IV (linearized)
lacZ
pEX1
pMAL-c5E
pSpark V (linearized)
lambda
pEX2
pMAL-c5G
pSpot1
LITMUS28
pEX3
pMAL-c5X
pSpot2
LITMUS28i
pEZSeq-Amp
pMAL-c5X-His
pSpot3
LITMUS29
pEZSeq-Amp (linearized)
pMAL-c6T
pSpot4
LITMUS38
pEZSeq-Kan
pMAL-p5E
pSTBlue-1
LITMUS38i
pEZSeq-Kan (linearized)
pMAL-p5G
pSTV28
LITMUS39
pEZ BAC
pMAL-p5X
pSTV29
M13mp18
pEZ BAC (BamHI linearized)
pMCS5
pT7Blue
M13mp19
pEZ BAC (HpaI linearized)
pMiniT
pT7Blue-2
MBP
pF1A T7
pMiniT (linearized)
pT7Blue-3
pACYC177
pF1K T7
pMiniT 2.0
pT7CFE1-CFtag
pACYC184
pF25A ICE T7
pMiniT 2.0 (linearized)
pT7CFE1-CGFP-HA-His
pAN7-1
pF25K ICE T7
pMMB190
pT7CFE1-CGST-HA-His
pANT7_cGST
pF3A WG (BYDV)
pMMB206
pT7CFE1-CHA
pANT7_nHA
pF3K WG (BYDV)
pMMB207
pT7CFE1-CHis
pAUR316
pFC20A HaloTag T7 SP6
pMMB208
pT7CFE1-CMyc
pBC KS(+)
pFC20K HaloTag T7 SP6
pMMB66EH
pT7CFE1-NFtag
pBC KS(-)
pFC30A His6HaloTag T7
pMMB66HE
pT7CFE1-NHA
pBC SK(+)
pFC30K His6HaloTag T7
pMMB67EH
pT7CFE1-NHA-CHis
pBC SK(-)
pFC7A (HQ)
pMMB67HE
pT7CFE1-NHis
pBeloBAC11
pFC7K (HQ)
pNEB193
pT7CFE1-NHis-GST
pBluescript II KS(+)
pFN18A HaloTag T7
pNEB206A (linearized)
pT7CFE1-NHis-GST-CHA
pBluescript II KS(-)
pFN18K HaloTag T7
pOSIP-CH
pT7CFE1-NMyc
pBluescript II SK(+)
pFN19A HaloTag T7 SP6
pOSIP-CO
pTOP Blunt V2 (linearized)
pBluescript II SK(-)
pFN19K HaloTag T7 SP6
pOSIP-CT
pTrcHis A
pBluescript KS(+)
pFN29A His6HaloTag T7
pOSIP-KC
pTrcHis B
pBluescript KS(-)
pFN29K His6HaloTag T7
pOSIP-KH
pTrcHis C
pBluescript SK(+)
pFN2A (GST)
pOSIP-KL
pTrcHis2 A
pBluescript SK(-)
pFN2K (GST)
pOSIP-KO
pTrcHis2 B
pBR322
pFN6A (HQ)
pOSIP-KP
pTrcHis2 C
pBS KS(+)
pFN6K (HQ)
pOSIP-KT
pTV 118N
pBS KS(-)
pGEM-11Zf(+)
pOSIP-TH
pTWV228
pBS SK(+)
pGEM-11Zf(-)
pOSIP-TO
pTXB1
pBS SK(-)
pGEM-3Z
pOSIP-TT
pTYB21
pBS(+)
pGEM-3Zf(+)
pPCR-Script Amp SK(+)
pType IIs
pBS(-)
pGEM-3Zf(-)
pPCR-Script Cam SK(+)
pTZ19R
pBSK(+) Simple-Amp
pGEM-4Z
pPTR I
pTZ19U
pBSK(+) Simple-Kan
pGEM-5Zf(+)
pPTR II
pTZ57R
pcDNA3
pGEM-7Zf(+)
pRACE (linearized)
pUAST
pcDNA3.1(+)
pGEM-7Zf(-)
pRANGER-BTB-1
pUC118
pcDNA3.1(-)
pGEM-9Zf(-)
pRANGER-BTB-2
pUC119
pCold I
pGEM-T
pRANGER-BTB-3
pUC18
pCold II
pGEM-T (linearized)
pRANGER-BTB-5
pUC19
pCold III
pGEM-T Easy
pRHA-109
pUC19L
pCold IV
pGEM-T Easy (linearized)
pRHA-113
pUC57
pCold TF
pGGA
pRHA-67
pUC57-Kan
pCR-XL-2-TOPO
pGGAselect
pRSET A
pUC57-Simple
pCR-XL-2-TOPO (linearized)
PGK promoter
pRSET B
pUCmu
pDD
pGS-21a
pRSET C
pVLT31
pDNR-Dual
pH6HTC His6HaloTag T7
pSB1C3
pVLT33
pDNR-LIB
pH6HTN His6HaloTag T7
pSC101
pVLT35
pE-SUMOpro Amp
pHP45(omega)
pSEAP2-Basic
pYES1L
pE-SUMOpro Kan
pHP45(omega)-Km
pSF-Core
pZErO-2
pE-SUMOpro3 Amp
pHP45(omega)-Tc
pSMART BAC v2.0
SNAP-tag
pE-SUMOpro3 Kan
pHSG298
pSMART BAC v2.0 (BamHI linearized)
SP6 promoter
pE-SUMOstar Amp
pHSG299
pSMART BAC v2.0 (EcoRI linearized)
SV40 promoter
pE-SUMOstar Kan
pHSG396
pSMART BAC v2.0 (HindIII linearized)
T3 promoter
pEASY-Blunt (linearized)
pHSG398
pSMART FOS v2.0 (linearized)
T5 promoter
pEASY-Blunt E1 (linearized)
pHY300PLK
pSMART HCAmp
T7 promoter
pEASY-Blunt E2 (linearized)
pICOz
pSMART HCAmp (linearized)
tac promoter
pEASY-Blunt Simple (linearized)
pInvRecA
pSMART HCKan
trc promoter
pEASY-Blunt Zero (linearized)
pJAZZ-OC
pSMART HCKan (linearized)
phiX174
pEASY-Blunt3 (linearized)
pJAZZ-OK

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Fluorescent Protein Genes & Plasmids
Lucigen Vectors
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Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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