pYES1L

Linearized BAC/YAC shuttle vector for assembling large DNA fragments using the GeneArt® High-Order Genetic Assembly System.

Sequence Author: Thermo Fisher (Invitrogen)

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HomePlasmidsBasic Cloning VectorspYES1L
8000 6000 4000 2000 End (9380) NotI (9360) I-SceI (9322) AmpR promoter I-CeuI (59) PacI (16) Start (0) TRP1 TRP1 promoter CEN/ARS SmR ori2 repE incC sopA sopC oriT sopB pYES1L 9380 bp
End  (9380)
0 sites
NotI  (9360)
1 site		 G C G G C C G C C G C C G G C G
I-SceI  (9322)
1 site		 T A G G G A T A A C A G G G T A A T A T C C C T A T T G T C C C A T T A
I-SceI is a homing endonuclease that can recognize a variety of similar recognition sequences.
After cleavage, I-SceI can remain bound to DNA and alter its electrophoretic mobility.
I-CeuI  (59)
1 site		 T A A C T A T A A C G G T C C T A A G G T A G C G A A T T G A T A T T G C C A G G A T T C C A T C G C T
I-CeuI is a homing endonuclease that can recognize a variety of similar recognition sequences.
PacI  (16)
1 site		 T T A A T T A A A A T T A A T T
Start  (0)
0 sites
TRP1
172 .. 846  =  675 bp
224 amino acids  =  24.2 kDa
Product: phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis
yeast auxotrophic marker
TRP1
172 .. 846  =  675 bp
224 amino acids  =  24.2 kDa
Product: phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis
yeast auxotrophic marker
TRP1 promoter
847 .. 1127  =  281 bp
TRP1 promoter
847 .. 1127  =  281 bp
CEN/ARS
1386 .. 1889  =  504 bp
S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence
CEN/ARS
1386 .. 1889  =  504 bp
S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence
AmpR promoter
1926 .. 2030  =  105 bp
AmpR promoter
1926 .. 2030  =  105 bp
SmR
2514 .. 3305  =  792 bp
263 amino acids  =  29.2 kDa
Product: aminoglycoside adenylyltransferase (Murphy, 1985)
confers resistance to spectinomycin and streptomycin
SmR
2514 .. 3305  =  792 bp
263 amino acids  =  29.2 kDa
Product: aminoglycoside adenylyltransferase (Murphy, 1985)
confers resistance to spectinomycin and streptomycin
ori2
4367 .. 4586  =  220 bp
secondary origin of replication for the bacterial F plasmid; also known as oriS
ori2
4367 .. 4586  =  220 bp
secondary origin of replication for the bacterial F plasmid; also known as oriS
repE
4677 .. 5432  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the bacterial F plasmid
repE
4677 .. 5432  =  756 bp
251 amino acids  =  29.4 kDa
Product: replication initiation protein for the bacterial F plasmid
incC
5435 .. 5685  =  251 bp
incompatibility region of the bacterial F plasmid
incC
5435 .. 5685  =  251 bp
incompatibility region of the bacterial F plasmid
sopA
6011 .. 7186  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F plasmid
sopA
6011 .. 7186  =  1176 bp
391 amino acids  =  44.1 kDa
Product: partitioning protein for the bacterial F plasmid
sopC
8230 .. 8703  =  474 bp
centromere-like partitioning region of the bacterial F plasmid
sopC
8230 .. 8703  =  474 bp
centromere-like partitioning region of the bacterial F plasmid
oriT
8994 .. 9284  =  291 bp
origin of transfer for the bacterial F plasmid (Frost et al., 1994)
oriT
8994 .. 9284  =  291 bp
origin of transfer for the bacterial F plasmid (Frost et al., 1994)
sopB
7186 .. 8157  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F plasmid
sopB
7186 .. 8157  =  972 bp
323 amino acids  =  35.4 kDa
Product: partitioning protein for the bacterial F plasmid
ORF:  3556 .. 3903  =  348 bp
ORF:  115 amino acids  =  12.9 kDa
ORF:  4666 .. 4896  =  231 bp
ORF:  76 amino acids  =  8.7 kDa
ORF:  4981 .. 5226  =  246 bp
ORF:  81 amino acids  =  8.9 kDa
ORF:  7186 .. 8157  =  972 bp
ORF:  323 amino acids  =  35.4 kDa
ORF:  74 .. 436  =  363 bp
ORF:  120 amino acids  =  13.3 kDa
ORF:  6011 .. 7186  =  1176 bp
ORF:  391 amino acids  =  44.1 kDa
ORF:  7538 .. 7936  =  399 bp
ORF:  132 amino acids  =  15.6 kDa
ORF:  2295 .. 3305  =  1011 bp
ORF:  336 amino acids  =  37.4 kDa
ORF:  4677 .. 5432  =  756 bp
ORF:  251 amino acids  =  29.4 kDa
ORF:  6192 .. 6344  =  153 bp
ORF:  50 amino acids  =  6.0 kDa
ORF:  315 .. 596  =  282 bp
ORF:  93 amino acids  =  10.8 kDa
ORF:  2553 .. 2936  =  384 bp
ORF:  127 amino acids  =  14.6 kDa
ORF:  4833 .. 5066  =  234 bp
ORF:  77 amino acids  =  9.1 kDa
ORF:  7227 .. 7463  =  237 bp
ORF:  78 amino acids  =  8.2 kDa
ORF:  2156 .. 2365  =  210 bp
ORF:  69 amino acids  =  8.1 kDa
ORF:  2546 .. 2803  =  258 bp
ORF:  85 amino acids  =  9.1 kDa
ORF:  2813 .. 3091  =  279 bp
ORF:  92 amino acids  =  9.4 kDa
ORF:  5912 .. 6064  =  153 bp
ORF:  50 amino acids  =  5.8 kDa
ORF:  6212 .. 6361  =  150 bp
ORF:  49 amino acids  =  5.8 kDa
ORF:  172 .. 846  =  675 bp
ORF:  224 amino acids  =  24.2 kDa
ORF:  1144 .. 1302  =  159 bp
ORF:  52 amino acids  =  5.8 kDa
ORF:  2059 .. 2256  =  198 bp
ORF:  65 amino acids  =  7.3 kDa
ORF:  3499 .. 3780  =  282 bp
ORF:  93 amino acids  =  10.7 kDa
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Individual Sequences & Maps

BlueScribe
pEASY-T1 (linearized)
pJET1.2
pSMART LCAmp
CAG promoter
pEASY-T1 Simple (linearized)
pJET1.2 blunt
pSMART LCAmp (linearized)
chicken beta-actin promoter
pEASY-T3 (linearized)
pJW168
pSMART LCKan
CLIP-tag
pEASY-T5 Zero (linearized)
pKF 18k-2
pSMART LCKan (linearized)
CMV promoter
pEX-1
pKF 19k-2
pSP64 Poly(A)
GAL1 promoter
pEX-A
pKOV
pSP72
GST
pEX-C-GST
pLATE11 (linearized)
pSP73
HaloTag
pEX-C-His
pLATE31 (linearized)
pSpark Done (linearized)
HSV TK promoter
pEX-K
pLATE51 (linearized)
pSpark I (linearized)
lac operator
pEX-N-GST
pLATE52 (linearized)
pSpark II (linearized)
lac promoter
pEX-N-His
pLEXX-AK
pSpark III (linearized)
lac UV5 promoter
pEX-N-His-GST
pLPS-B (linearized)
pSpark IV (linearized)
lacZ
pEX1
pMAL-c5E
pSpark V (linearized)
lambda
pEX2
pMAL-c5G
pSpot1
LITMUS28
pEX3
pMAL-c5X
pSpot2
LITMUS28i
pEZSeq-Amp
pMAL-c5X-His
pSpot3
LITMUS29
pEZSeq-Amp (linearized)
pMAL-c6T
pSpot4
LITMUS38
pEZSeq-Kan
pMAL-p5E
pSTBlue-1
LITMUS38i
pEZSeq-Kan (linearized)
pMAL-p5G
pSTV28
LITMUS39
pEZ BAC
pMAL-p5X
pSTV29
M13mp18
pEZ BAC (BamHI linearized)
pMCS5
pT7Blue
M13mp19
pEZ BAC (HpaI linearized)
pMiniT
pT7Blue-2
MBP
pF1A T7
pMiniT (linearized)
pT7Blue-3
pACYC177
pF1K T7
pMiniT 2.0
pT7CFE1-CFtag
pACYC184
pF25A ICE T7
pMiniT 2.0 (linearized)
pT7CFE1-CGFP-HA-His
pAN7-1
pF25K ICE T7
pMMB190
pT7CFE1-CGST-HA-His
pANT7_cGST
pF3A WG (BYDV)
pMMB206
pT7CFE1-CHA
pANT7_nHA
pF3K WG (BYDV)
pMMB207
pT7CFE1-CHis
pAUR316
pFC20A HaloTag T7 SP6
pMMB208
pT7CFE1-CMyc
pBC KS(+)
pFC20K HaloTag T7 SP6
pMMB66EH
pT7CFE1-NFtag
pBC KS(-)
pFC30A His6HaloTag T7
pMMB66HE
pT7CFE1-NHA
pBC SK(+)
pFC30K His6HaloTag T7
pMMB67EH
pT7CFE1-NHA-CHis
pBC SK(-)
pFC7A (HQ)
pMMB67HE
pT7CFE1-NHis
pBeloBAC11
pFC7K (HQ)
pNEB193
pT7CFE1-NHis-GST
pBluescript II KS(+)
pFN18A HaloTag T7
pNEB206A (linearized)
pT7CFE1-NHis-GST-CHA
pBluescript II KS(-)
pFN18K HaloTag T7
pOSIP-CH
pT7CFE1-NMyc
pBluescript II SK(+)
pFN19A HaloTag T7 SP6
pOSIP-CO
pTOP Blunt V2 (linearized)
pBluescript II SK(-)
pFN19K HaloTag T7 SP6
pOSIP-CT
pTrcHis A
pBluescript KS(+)
pFN29A His6HaloTag T7
pOSIP-KC
pTrcHis B
pBluescript KS(-)
pFN29K His6HaloTag T7
pOSIP-KH
pTrcHis C
pBluescript SK(+)
pFN2A (GST)
pOSIP-KL
pTrcHis2 A
pBluescript SK(-)
pFN2K (GST)
pOSIP-KO
pTrcHis2 B
pBR322
pFN6A (HQ)
pOSIP-KP
pTrcHis2 C
pBS KS(+)
pFN6K (HQ)
pOSIP-KT
pTV 118N
pBS KS(-)
pGEM-11Zf(+)
pOSIP-TH
pTWV228
pBS SK(+)
pGEM-11Zf(-)
pOSIP-TO
pTXB1
pBS SK(-)
pGEM-3Z
pOSIP-TT
pTYB21
pBS(+)
pGEM-3Zf(+)
pPCR-Script Amp SK(+)
pType IIs
pBS(-)
pGEM-3Zf(-)
pPCR-Script Cam SK(+)
pTZ19R
pBSK(+) Simple-Amp
pGEM-4Z
pPTR I
pTZ19U
pBSK(+) Simple-Kan
pGEM-5Zf(+)
pPTR II
pTZ57R
pcDNA3
pGEM-7Zf(+)
pRACE (linearized)
pUAST
pcDNA3.1(+)
pGEM-7Zf(-)
pRANGER-BTB-1
pUC118
pcDNA3.1(-)
pGEM-9Zf(-)
pRANGER-BTB-2
pUC119
pCold I
pGEM-T
pRANGER-BTB-3
pUC18
pCold II
pGEM-T (linearized)
pRANGER-BTB-5
pUC19
pCold III
pGEM-T Easy
pRHA-109
pUC19L
pCold IV
pGEM-T Easy (linearized)
pRHA-113
pUC57
pCold TF
pGGA
pRHA-67
pUC57-Kan
pCR-XL-2-TOPO
pGGAselect
pRSET A
pUC57-Simple
pCR-XL-2-TOPO (linearized)
PGK promoter
pRSET B
pUCmu
pDD
pGS-21a
pRSET C
pVLT31
pDNR-Dual
pH6HTC His6HaloTag T7
pSB1C3
pVLT33
pDNR-LIB
pH6HTN His6HaloTag T7
pSC101
pVLT35
pE-SUMOpro Amp
pHP45(omega)
pSEAP2-Basic
pYES1L
pE-SUMOpro Kan
pHP45(omega)-Km
pSF-Core
pZErO-2
pE-SUMOpro3 Amp
pHP45(omega)-Tc
pSMART BAC v2.0
SNAP-tag
pE-SUMOpro3 Kan
pHSG298
pSMART BAC v2.0 (BamHI linearized)
SP6 promoter
pE-SUMOstar Amp
pHSG299
pSMART BAC v2.0 (EcoRI linearized)
SV40 promoter
pE-SUMOstar Kan
pHSG396
pSMART BAC v2.0 (HindIII linearized)
T3 promoter
pEASY-Blunt (linearized)
pHSG398
pSMART FOS v2.0 (linearized)
T5 promoter
pEASY-Blunt E1 (linearized)
pHY300PLK
pSMART HCAmp
T7 promoter
pEASY-Blunt E2 (linearized)
pICOz
pSMART HCAmp (linearized)
tac promoter
pEASY-Blunt Simple (linearized)
pInvRecA
pSMART HCKan
trc promoter
pEASY-Blunt Zero (linearized)
pJAZZ-OC
pSMART HCKan (linearized)
phiX174
pEASY-Blunt3 (linearized)
pJAZZ-OK

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Lucigen Vectors
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Yeast Plasmids
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Mammalian Expression Vectors
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