pInvRecA

Plasmid for transiently converting any E. coli strain to a RecA+ phenotype for phage P1-mediated transduction.

Sequence Author: Lucigen

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HomePlasmidsBasic Cloning VectorspInvRecA
BaeGI - Bme1580I * (7102) ApaLI (7098) ScaI (6911) PvuI (6801) FspI (6653) NmeAIII (6579) BglI (6551) BsaI (6492) AhdI (6431) PluTI (5991) SfoI (5989) NarI (5988) KasI (5987) AflII (5824) AflIII - PciI (5066) BstXI (4416) AatII (4289) ZraI (4287) rrnB T1 terminator rrnB T1 terminator rrnB T1 terminator HpaI (12) BsgI (131) AleI (153) AfeI (287) EcoNI (482) Bpu10I (527) Bsu36I (814) SwaI (1388) MscI * (1794) BmgBI (2134) NheI (2198) BmtI (2202) AvaI - BsoBI - PaeR7I - XhoI (2222) BlpI (2384) PmeI (2604) BsmBI - Esp3I - PshAI (2695) DrdI (2950) SgrAI (3004) PstI (3140) RsrII (3170) BglII (3177) BamHI (3412) FRT rrnB T1 terminator pInvRecA 7354 bp
BaeGI  (7102)
1 site		 G K G C M C C M C G K G
Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (7102)
1 site		 G K G C M C C M C G K G
* Blocked by EcoKI methylation.
Sticky ends from different Bme1580I sites may not be compatible.
ApaLI  (7098)
1 site		 G T G C A C C A C G T G
ScaI  (6911)
1 site		 A G T A C T T C A T G A
PvuI  (6801)
1 site		 C G A T C G G C T A G C
FspI  (6653)
1 site		 T G C G C A A C G C G T
NmeAIII  (6579)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (6551)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
BsaI  (6492)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (6431)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PluTI  (5991)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (5989)
1 site		 G G C G C C C C G C G G
NarI  (5988)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (5987)
1 site		 G G C G C C C C G C G G
AflII  (5824)
1 site		 C T T A A G G A A T T C
AflIII  (5066)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (5066)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
BstXI  (4416)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
AatII  (4289)
1 site		 G A C G T C C T G C A G
ZraI  (4287)
1 site		 G A C G T C C T G C A G
HpaI  (12)
1 site		 G T T A A C C A A T T G
BsgI  (131)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AleI  (153)
1 site		 C A C N N N N G T G G T G N N N N C A C
AfeI  (287)
1 site		 A G C G C T T C G C G A
EcoNI  (482)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
Bpu10I  (527)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
Bsu36I  (814)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
SwaI  (1388)
1 site		 A T T T A A A T T A A A T T T A
SwaI is typically used at 25°C, but is 50% active at 37°C.
MscI  (1794)
1 site		 T G G C C A A C C G G T
* Blocked by Dcm methylation.
BmgBI  (2134)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
NheI  (2198)
1 site		 G C T A G C C G A T C G
BmtI  (2202)
1 site		 G C T A G C C G A T C G
AvaI  (2222)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BsoBI  (2222)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (2222)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2222)
1 site		 C T C G A G G A G C T C
BlpI  (2384)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
PmeI  (2604)
1 site		 G T T T A A A C C A A A T T T G
BsmBI  (2695)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (2695)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
PshAI  (2695)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
DrdI  (2950)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
SgrAI  (3004)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
PstI  (3140)
1 site		 C T G C A G G A C G T C
RsrII  (3170)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BglII  (3177)
1 site		 A G A T C T T C T A G A
BamHI  (3412)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
FLP
783 .. 2051  =  1269 bp
422 amino acids  =  48.5 kDa
Product: site-specific recombinase
FLP is a site-specific recombinase from Saccharomyces cerevisiae. Recombination occurs at FRT sequences.
FLP
783 .. 2051  =  1269 bp
422 amino acids  =  48.5 kDa
Product: site-specific recombinase
FLP is a site-specific recombinase from Saccharomyces cerevisiae. Recombination occurs at FRT sequences.
RecA
2311 .. 3372  =  1062 bp
353 amino acids  =  38.0 kDa
Product: DNA repair protein from E. coli
RecA
2311 .. 3372  =  1062 bp
353 amino acids  =  38.0 kDa
Product: DNA repair protein from E. coli
Rep101(Ts)
4516 .. 5466  =  951 bp
316 amino acids  =  37.4 kDa
Product: temperature-sensitive version of a protein needed for replication with the pSC101 origin
Rep101(Ts)
4516 .. 5466  =  951 bp
316 amino acids  =  37.4 kDa
Product: temperature-sensitive version of a protein needed for replication with the pSC101 origin
AmpR
6358 .. 7218  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   6358 .. 7149  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6358 .. 7218  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   7150 .. 7218  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6358 .. 7218  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
TetR
69 .. 692  =  624 bp
207 amino acids  =  23.4 kDa
Product: tetracycline repressor TetR
TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline.
TetR
69 .. 692  =  624 bp
207 amino acids  =  23.4 kDa
Product: tetracycline repressor TetR
TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline.
pSC101 ori
5514 .. 5736  =  223 bp
low-copy replication origin that requires the Rep101 protein
pSC101 ori
5514 .. 5736  =  223 bp
low-copy replication origin that requires the Rep101 protein
AmpR promoter
7219 .. 7323  =  105 bp
AmpR promoter
7219 .. 7323  =  105 bp
rrnB T1 terminator
3562 .. 3648  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
3562 .. 3648  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
3743 .. 3829  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
3743 .. 3829  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
3924 .. 4010  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
3924 .. 4010  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
4105 .. 4191  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
4105 .. 4191  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
tetR/tetA promoters
711 .. 766  =  56 bp
overlapping promoters for bacterial tetR and tetA
tetR/tetA promoters
711 .. 766  =  56 bp
overlapping promoters for bacterial tetR and tetA
FRT
2251 .. 2284  =  34 bp
FLP-mediated recombination occurs in the 8-bp core sequence (TTTCTAGA).
FRT
2251 .. 2284  =  34 bp
FLP-mediated recombination occurs in the 8-bp core sequence (TTTCTAGA).
FRT
3425 .. 3458  =  34 bp
FLP-mediated recombination occurs in the 8-bp core sequence (TTTCTAGA).
FRT
3425 .. 3458  =  34 bp
FLP-mediated recombination occurs in the 8-bp core sequence (TTTCTAGA).
tet operator
717 .. 735  =  19 bp
bacterial operator O1 for the tetR and tetA genes
tet operator
717 .. 735  =  19 bp
bacterial operator O1 for the tetR and tetA genes
tet operator
747 .. 765  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
747 .. 765  =  19 bp
bacterial operator O2 for the tetR and tetA genes
ORF:  6488 .. 6754  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  774 .. 2051  =  1278 bp
ORF:  425 amino acids  =  48.8 kDa
ORF:  1115 .. 1468  =  354 bp
ORF:  117 amino acids  =  13.5 kDa
ORF:  2311 .. 3372  =  1062 bp
ORF:  353 amino acids  =  38.0 kDa
ORF:  4036 .. 4266  =  231 bp
ORF:  76 amino acids  =  8.8 kDa
ORF:  4516 .. 5466  =  951 bp
ORF:  316 amino acids  =  37.4 kDa
ORF:  6358 .. 7218  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  69 .. 695  =  627 bp
ORF:  208 amino acids  =  23.5 kDa
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Individual Sequences & Maps

BlueScribe
pEASY-T1 (linearized)
pJET1.2
pSMART LCAmp
CAG promoter
pEASY-T1 Simple (linearized)
pJET1.2 blunt
pSMART LCAmp (linearized)
chicken beta-actin promoter
pEASY-T3 (linearized)
pJW168
pSMART LCKan
CLIP-tag
pEASY-T5 Zero (linearized)
pKF 18k-2
pSMART LCKan (linearized)
CMV promoter
pEX-1
pKF 19k-2
pSP64 Poly(A)
GAL1 promoter
pEX-A
pKOV
pSP72
GST
pEX-C-GST
pLATE11 (linearized)
pSP73
HaloTag
pEX-C-His
pLATE31 (linearized)
pSpark Done (linearized)
HSV TK promoter
pEX-K
pLATE51 (linearized)
pSpark I (linearized)
lac operator
pEX-N-GST
pLATE52 (linearized)
pSpark II (linearized)
lac promoter
pEX-N-His
pLEXX-AK
pSpark III (linearized)
lac UV5 promoter
pEX-N-His-GST
pLPS-B (linearized)
pSpark IV (linearized)
lacZ
pEX1
pMAL-c5E
pSpark V (linearized)
lambda
pEX2
pMAL-c5G
pSpot1
LITMUS28
pEX3
pMAL-c5X
pSpot2
LITMUS28i
pEZSeq-Amp
pMAL-c5X-His
pSpot3
LITMUS29
pEZSeq-Amp (linearized)
pMAL-c6T
pSpot4
LITMUS38
pEZSeq-Kan
pMAL-p5E
pSTBlue-1
LITMUS38i
pEZSeq-Kan (linearized)
pMAL-p5G
pSTV28
LITMUS39
pEZ BAC
pMAL-p5X
pSTV29
M13mp18
pEZ BAC (BamHI linearized)
pMCS5
pT7Blue
M13mp19
pEZ BAC (HpaI linearized)
pMiniT
pT7Blue-2
MBP
pF1A T7
pMiniT (linearized)
pT7Blue-3
pACYC177
pF1K T7
pMiniT 2.0
pT7CFE1-CFtag
pACYC184
pF25A ICE T7
pMiniT 2.0 (linearized)
pT7CFE1-CGFP-HA-His
pAN7-1
pF25K ICE T7
pMMB190
pT7CFE1-CGST-HA-His
pANT7_cGST
pF3A WG (BYDV)
pMMB206
pT7CFE1-CHA
pANT7_nHA
pF3K WG (BYDV)
pMMB207
pT7CFE1-CHis
pAUR316
pFC20A HaloTag T7 SP6
pMMB208
pT7CFE1-CMyc
pBC KS(+)
pFC20K HaloTag T7 SP6
pMMB66EH
pT7CFE1-NFtag
pBC KS(-)
pFC30A His6HaloTag T7
pMMB66HE
pT7CFE1-NHA
pBC SK(+)
pFC30K His6HaloTag T7
pMMB67EH
pT7CFE1-NHA-CHis
pBC SK(-)
pFC7A (HQ)
pMMB67HE
pT7CFE1-NHis
pBeloBAC11
pFC7K (HQ)
pNEB193
pT7CFE1-NHis-GST
pBluescript II KS(+)
pFN18A HaloTag T7
pNEB206A (linearized)
pT7CFE1-NHis-GST-CHA
pBluescript II KS(-)
pFN18K HaloTag T7
pOSIP-CH
pT7CFE1-NMyc
pBluescript II SK(+)
pFN19A HaloTag T7 SP6
pOSIP-CO
pTOP Blunt V2 (linearized)
pBluescript II SK(-)
pFN19K HaloTag T7 SP6
pOSIP-CT
pTrcHis A
pBluescript KS(+)
pFN29A His6HaloTag T7
pOSIP-KC
pTrcHis B
pBluescript KS(-)
pFN29K His6HaloTag T7
pOSIP-KH
pTrcHis C
pBluescript SK(+)
pFN2A (GST)
pOSIP-KL
pTrcHis2 A
pBluescript SK(-)
pFN2K (GST)
pOSIP-KO
pTrcHis2 B
pBR322
pFN6A (HQ)
pOSIP-KP
pTrcHis2 C
pBS KS(+)
pFN6K (HQ)
pOSIP-KT
pTV 118N
pBS KS(-)
pGEM-11Zf(+)
pOSIP-TH
pTWV228
pBS SK(+)
pGEM-11Zf(-)
pOSIP-TO
pTXB1
pBS SK(-)
pGEM-3Z
pOSIP-TT
pTYB21
pBS(+)
pGEM-3Zf(+)
pPCR-Script Amp SK(+)
pType IIs
pBS(-)
pGEM-3Zf(-)
pPCR-Script Cam SK(+)
pTZ19R
pBSK(+) Simple-Amp
pGEM-4Z
pPTR I
pTZ19U
pBSK(+) Simple-Kan
pGEM-5Zf(+)
pPTR II
pTZ57R
pcDNA3
pGEM-7Zf(+)
pRACE (linearized)
pUAST
pcDNA3.1(+)
pGEM-7Zf(-)
pRANGER-BTB-1
pUC118
pcDNA3.1(-)
pGEM-9Zf(-)
pRANGER-BTB-2
pUC119
pCold I
pGEM-T
pRANGER-BTB-3
pUC18
pCold II
pGEM-T (linearized)
pRANGER-BTB-5
pUC19
pCold III
pGEM-T Easy
pRHA-109
pUC19L
pCold IV
pGEM-T Easy (linearized)
pRHA-113
pUC57
pCold TF
pGGA
pRHA-67
pUC57-Kan
pCR-XL-2-TOPO
pGGAselect
pRSET A
pUC57-Simple
pCR-XL-2-TOPO (linearized)
PGK promoter
pRSET B
pUCmu
pDD
pGS-21a
pRSET C
pVLT31
pDNR-Dual
pH6HTC His6HaloTag T7
pSB1C3
pVLT33
pDNR-LIB
pH6HTN His6HaloTag T7
pSC101
pVLT35
pE-SUMOpro Amp
pHP45(omega)
pSEAP2-Basic
pYES1L
pE-SUMOpro Kan
pHP45(omega)-Km
pSF-Core
pZErO-2
pE-SUMOpro3 Amp
pHP45(omega)-Tc
pSMART BAC v2.0
SNAP-tag
pE-SUMOpro3 Kan
pHSG298
pSMART BAC v2.0 (BamHI linearized)
SP6 promoter
pE-SUMOstar Amp
pHSG299
pSMART BAC v2.0 (EcoRI linearized)
SV40 promoter
pE-SUMOstar Kan
pHSG396
pSMART BAC v2.0 (HindIII linearized)
T3 promoter
pEASY-Blunt (linearized)
pHSG398
pSMART FOS v2.0 (linearized)
T5 promoter
pEASY-Blunt E1 (linearized)
pHY300PLK
pSMART HCAmp
T7 promoter
pEASY-Blunt E2 (linearized)
pICOz
pSMART HCAmp (linearized)
tac promoter
pEASY-Blunt Simple (linearized)
pInvRecA
pSMART HCKan
trc promoter
pEASY-Blunt Zero (linearized)
pJAZZ-OC
pSMART HCKan (linearized)
phiX174
pEASY-Blunt3 (linearized)
pJAZZ-OK

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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