pSC101

Low copy number plasmid from Salmonella. pSC101 was the first plasmid vector used for cloning.
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HomePlasmidsBasic Cloning VectorspSC101
EcoRI (9261) NsiI (9011) BsrDI (9009) AscI (8738) RsrII (8700) BaeGI - Bme1580I (8490) PaeR7I - PspXI - XhoI (8187) PvuII (7793) SacII (7564) DraIII (7346) CsiI - SexAI * (7342) MluI (7181) BstXI (7169) BssSI - BssSαI (7123) PciI (5668) EarI (5607) SpeI (5201) AflII (4910) HindIII (29) BamHI (375) SgrAI (410) SalI (651) PshAI (716) MscI * (1446) AgeI (1687) DrdI (2100) BsrGI (2159) PacI (2288) PflFI - Tth111I (2744) XmnI (2840) HpaI (3097) BsmBI - Esp3I (3522) SspI (4875) pSC101 9263 bp
EcoRI  (9261)
1 site		 G A A T T C C T T A A G
NsiI  (9011)
1 site		 A T G C A T T A C G T A
BsrDI  (9009)
1 site		 G C A A T G N N C G T T A C
Sticky ends from different BsrDI sites may not be compatible.
AscI  (8738)
1 site		 G G C G C G C C C C G C G C G G
RsrII  (8700)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BaeGI  (8490)
1 site		 G K G C M C C M C G K G
Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (8490)
1 site		 G K G C M C C M C G K G
Sticky ends from different Bme1580I sites may not be compatible.
PaeR7I  (8187)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (8187)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (8187)
1 site		 C T C G A G G A G C T C
PvuII  (7793)
1 site		 C A G C T G G T C G A C
SacII  (7564)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
DraIII  (7346)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
CsiI  (7342)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (7342)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
MluI  (7181)
1 site		 A C G C G T T G C G C A
BstXI  (7169)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
BssSI  (7123)
1 site		 C A C G A G G T G C T C
BssSαI  (7123)
1 site		 C A C G A G G T G C T C
PciI  (5668)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
EarI  (5607)
1 site		 C T C T T C N G A G A A G N N N N
Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
SpeI  (5201)
1 site		 A C T A G T T G A T C A
AflII  (4910)
1 site		 C T T A A G G A A T T C
HindIII  (29)
1 site		 A A G C T T T T C G A A
BamHI  (375)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SgrAI  (410)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SalI  (651)
1 site		 G T C G A C C A G C T G
PshAI  (716)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
MscI  (1446)
1 site		 T G G C C A A C C G G T
* Blocked by Dcm methylation.
AgeI  (1687)
1 site		 A C C G G T T G G C C A
DrdI  (2100)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
BsrGI  (2159)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
PacI  (2288)
1 site		 T T A A T T A A A A T T A A T T
PflFI  (2744)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2744)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
XmnI  (2840)
1 site		 G A A N N N N T T C C T T N N N N A A G
HpaI  (3097)
1 site		 G T T A A C C A A T T G
BsmBI  (3522)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (3522)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
SspI  (4875)
1 site		 A A T A T T T T A T A A
TcR
86 .. 1276  =  1191 bp
396 amino acids  =  41.5 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
TcR
86 .. 1276  =  1191 bp
396 amino acids  =  41.5 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
Rep101
5273 .. 6223  =  951 bp
316 amino acids  =  37.3 kDa
Product: protein needed for replication with the pSC101 origin
Rep101
5273 .. 6223  =  951 bp
316 amino acids  =  37.3 kDa
Product: protein needed for replication with the pSC101 origin
pSC101 ori
5003 .. 5225  =  223 bp
low-copy replication origin that requires the Rep101 protein
pSC101 ori
5003 .. 5225  =  223 bp
low-copy replication origin that requires the Rep101 protein
tet promoter
10 .. 38  =  29 bp
3 segments
   Segment 1:  -35  
   10 .. 15  =  6 bp
promoter for tetracycline efflux protein gene
tet promoter
10 .. 38  =  29 bp
3 segments
   Segment 2:  
   16 .. 32  =  17 bp
promoter for tetracycline efflux protein gene
tet promoter
10 .. 38  =  29 bp
3 segments
   Segment 3:  -10  
   33 .. 38  =  6 bp
promoter for tetracycline efflux protein gene
tet promoter
10 .. 38  =  29 bp
3 segments
promoter for tetracycline efflux protein gene
ORF:  259 .. 642  =  384 bp
ORF:  127 amino acids  =  14.7 kDa
ORF:  1180 .. 1518  =  339 bp
ORF:  112 amino acids  =  12.6 kDa
ORF:  86 .. 1276  =  1191 bp
ORF:  396 amino acids  =  41.5 kDa
ORF:  2291 .. 2596  =  306 bp
ORF:  101 amino acids  =  11.5 kDa
ORF:  3410 .. 3808  =  399 bp
ORF:  132 amino acids  =  14.7 kDa
ORF:  5273 .. 6223  =  951 bp
ORF:  316 amino acids  =  37.3 kDa
ORF:  8957 .. 9184  =  228 bp
ORF:  75 amino acids  =  8.8 kDa
ORF:  3138 .. 3392  =  255 bp
ORF:  84 amino acids  =  8.9 kDa
ORF:  4302 .. 4679  =  378 bp
ORF:  125 amino acids  =  13.6 kDa
ORF:  7242 .. 7916  =  675 bp
ORF:  224 amino acids  =  25.3 kDa
ORF:  8478 .. 8714  =  237 bp
ORF:  78 amino acids  =  8.2 kDa
ORF:  1515 .. 1739  =  225 bp
ORF:  74 amino acids  =  8.2 kDa
ORF:  7122 .. 7466  =  345 bp
ORF:  114 amino acids  =  12.3 kDa
ORF:  323 .. 565  =  243 bp
ORF:  80 amino acids  =  8.0 kDa
ORF:  623 .. 1081  =  459 bp
ORF:  152 amino acids  =  16.7 kDa
ORF:  2339 .. 2923  =  585 bp
ORF:  194 amino acids  =  21.9 kDa
ORF:  2936 .. 4051  =  1116 bp
ORF:  371 amino acids  =  43.1 kDa
ORF:  4133 .. 4417  =  285 bp
ORF:  94 amino acids  =  10.1 kDa
ORF:  8597 .. 9256  =  660 bp
ORF:  219 amino acids  =  24.2 kDa
ORF:  439 .. 789  =  351 bp
ORF:  116 amino acids  =  12.2 kDa
ORF:  8296 .. 8610  =  315 bp
ORF:  104 amino acids  =  11.5 kDa
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Download pSC101.dna file

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Individual Sequences & Maps

BlueScribe
pEASY-T1 (linearized)
pJET1.2
pSMART LCAmp
CAG promoter
pEASY-T1 Simple (linearized)
pJET1.2 blunt
pSMART LCAmp (linearized)
chicken beta-actin promoter
pEASY-T3 (linearized)
pJW168
pSMART LCKan
CLIP-tag
pEASY-T5 Zero (linearized)
pKF 18k-2
pSMART LCKan (linearized)
CMV promoter
pEX-1
pKF 19k-2
pSP64 Poly(A)
GAL1 promoter
pEX-A
pKOV
pSP72
GST
pEX-C-GST
pLATE11 (linearized)
pSP73
HaloTag
pEX-C-His
pLATE31 (linearized)
pSpark Done (linearized)
HSV TK promoter
pEX-K
pLATE51 (linearized)
pSpark I (linearized)
lac operator
pEX-N-GST
pLATE52 (linearized)
pSpark II (linearized)
lac promoter
pEX-N-His
pLEXX-AK
pSpark III (linearized)
lac UV5 promoter
pEX-N-His-GST
pLPS-B (linearized)
pSpark IV (linearized)
lacZ
pEX1
pMAL-c5E
pSpark V (linearized)
lambda
pEX2
pMAL-c5G
pSpot1
LITMUS28
pEX3
pMAL-c5X
pSpot2
LITMUS28i
pEZSeq-Amp
pMAL-c5X-His
pSpot3
LITMUS29
pEZSeq-Amp (linearized)
pMAL-c6T
pSpot4
LITMUS38
pEZSeq-Kan
pMAL-p5E
pSTBlue-1
LITMUS38i
pEZSeq-Kan (linearized)
pMAL-p5G
pSTV28
LITMUS39
pEZ BAC
pMAL-p5X
pSTV29
M13mp18
pEZ BAC (BamHI linearized)
pMCS5
pT7Blue
M13mp19
pEZ BAC (HpaI linearized)
pMiniT
pT7Blue-2
MBP
pF1A T7
pMiniT (linearized)
pT7Blue-3
pACYC177
pF1K T7
pMiniT 2.0
pT7CFE1-CFtag
pACYC184
pF25A ICE T7
pMiniT 2.0 (linearized)
pT7CFE1-CGFP-HA-His
pAN7-1
pF25K ICE T7
pMMB190
pT7CFE1-CGST-HA-His
pANT7_cGST
pF3A WG (BYDV)
pMMB206
pT7CFE1-CHA
pANT7_nHA
pF3K WG (BYDV)
pMMB207
pT7CFE1-CHis
pAUR316
pFC20A HaloTag T7 SP6
pMMB208
pT7CFE1-CMyc
pBC KS(+)
pFC20K HaloTag T7 SP6
pMMB66EH
pT7CFE1-NFtag
pBC KS(-)
pFC30A His6HaloTag T7
pMMB66HE
pT7CFE1-NHA
pBC SK(+)
pFC30K His6HaloTag T7
pMMB67EH
pT7CFE1-NHA-CHis
pBC SK(-)
pFC7A (HQ)
pMMB67HE
pT7CFE1-NHis
pBeloBAC11
pFC7K (HQ)
pNEB193
pT7CFE1-NHis-GST
pBluescript II KS(+)
pFN18A HaloTag T7
pNEB206A (linearized)
pT7CFE1-NHis-GST-CHA
pBluescript II KS(-)
pFN18K HaloTag T7
pOSIP-CH
pT7CFE1-NMyc
pBluescript II SK(+)
pFN19A HaloTag T7 SP6
pOSIP-CO
pTOP Blunt V2 (linearized)
pBluescript II SK(-)
pFN19K HaloTag T7 SP6
pOSIP-CT
pTrcHis A
pBluescript KS(+)
pFN29A His6HaloTag T7
pOSIP-KC
pTrcHis B
pBluescript KS(-)
pFN29K His6HaloTag T7
pOSIP-KH
pTrcHis C
pBluescript SK(+)
pFN2A (GST)
pOSIP-KL
pTrcHis2 A
pBluescript SK(-)
pFN2K (GST)
pOSIP-KO
pTrcHis2 B
pBR322
pFN6A (HQ)
pOSIP-KP
pTrcHis2 C
pBS KS(+)
pFN6K (HQ)
pOSIP-KT
pTV 118N
pBS KS(-)
pGEM-11Zf(+)
pOSIP-TH
pTWV228
pBS SK(+)
pGEM-11Zf(-)
pOSIP-TO
pTXB1
pBS SK(-)
pGEM-3Z
pOSIP-TT
pTYB21
pBS(+)
pGEM-3Zf(+)
pPCR-Script Amp SK(+)
pType IIs
pBS(-)
pGEM-3Zf(-)
pPCR-Script Cam SK(+)
pTZ19R
pBSK(+) Simple-Amp
pGEM-4Z
pPTR I
pTZ19U
pBSK(+) Simple-Kan
pGEM-5Zf(+)
pPTR II
pTZ57R
pcDNA3
pGEM-7Zf(+)
pRACE (linearized)
pUAST
pcDNA3.1(+)
pGEM-7Zf(-)
pRANGER-BTB-1
pUC118
pcDNA3.1(-)
pGEM-9Zf(-)
pRANGER-BTB-2
pUC119
pCold I
pGEM-T
pRANGER-BTB-3
pUC18
pCold II
pGEM-T (linearized)
pRANGER-BTB-5
pUC19
pCold III
pGEM-T Easy
pRHA-109
pUC19L
pCold IV
pGEM-T Easy (linearized)
pRHA-113
pUC57
pCold TF
pGGA
pRHA-67
pUC57-Kan
pCR-XL-2-TOPO
pGGAselect
pRSET A
pUC57-Simple
pCR-XL-2-TOPO (linearized)
PGK promoter
pRSET B
pUCmu
pDD
pGS-21a
pRSET C
pVLT31
pDNR-Dual
pH6HTC His6HaloTag T7
pSB1C3
pVLT33
pDNR-LIB
pH6HTN His6HaloTag T7
pSC101
pVLT35
pE-SUMOpro Amp
pHP45(omega)
pSEAP2-Basic
pYES1L
pE-SUMOpro Kan
pHP45(omega)-Km
pSF-Core
pZErO-2
pE-SUMOpro3 Amp
pHP45(omega)-Tc
pSMART BAC v2.0
SNAP-tag
pE-SUMOpro3 Kan
pHSG298
pSMART BAC v2.0 (BamHI linearized)
SP6 promoter
pE-SUMOstar Amp
pHSG299
pSMART BAC v2.0 (EcoRI linearized)
SV40 promoter
pE-SUMOstar Kan
pHSG396
pSMART BAC v2.0 (HindIII linearized)
T3 promoter
pEASY-Blunt (linearized)
pHSG398
pSMART FOS v2.0 (linearized)
T5 promoter
pEASY-Blunt E1 (linearized)
pHY300PLK
pSMART HCAmp
T7 promoter
pEASY-Blunt E2 (linearized)
pICOz
pSMART HCAmp (linearized)
tac promoter
pEASY-Blunt Simple (linearized)
pInvRecA
pSMART HCKan
trc promoter
pEASY-Blunt Zero (linearized)
pJAZZ-OC
pSMART HCKan (linearized)
phiX174
pEASY-Blunt3 (linearized)
pJAZZ-OK

Plasmid Sets

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Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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