pFN18K HaloTag T7

Flexi® vector with a kanamycin resistance marker, for bacterial or cell-free expression of a protein with a cleavable N-terminal HaloTag®.

Sequence Author: Promega

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T7 promoter pFN18K HaloTag® T7 4372 bp
HaloTag®
70 .. 960  =  891 bp
297 amino acids  =  33.6 kDa
Product: modified bacterial dehalogenase that forms covalent bonds with chloroalkane derivatives
HaloTag®
70 .. 960  =  891 bp
297 amino acids  =  33.6 kDa
Product: modified bacterial dehalogenase that forms covalent bonds with chloroalkane derivatives
TEV site
973 .. 993  =  21 bp
7 amino acids  =  900.9 Da
Product: tobacco etch virus (TEV) protease recognition and cleavage site
TEV protease recognition site
TEV site
973 .. 993  =  21 bp
7 amino acids  =  900.9 Da
Product: tobacco etch virus (TEV) protease recognition and cleavage site
TEV protease recognition site
NeoR/KanR
1923 .. 2717  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
1923 .. 2717  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori
2889 .. 3477  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2889 .. 3477  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
barnase
1031 .. 1366  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
barnase
1031 .. 1366  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
cer region
3593 .. 3876  =  284 bp
ColE1-derived recombination site that helps to maintain plasmids as monomers
cer region
3593 .. 3876  =  284 bp
ColE1-derived recombination site that helps to maintain plasmids as monomers
rrnB T1 terminator
4106 .. 4192  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
4106 .. 4192  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
T7 terminator
1495 .. 1542  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
1495 .. 1542  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
rrnB T2 terminator
4284 .. 4311  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
rrnB T2 terminator
4284 .. 4311  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
T7 promoter
21 .. 39  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
21 .. 39  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  70 .. 1017  =  948 bp
ORF:  315 amino acids  =  35.7 kDa
ORF:  2095 .. 2481  =  387 bp
ORF:  128 amino acids  =  14.7 kDa
ORF:  3838 .. 4185  =  348 bp
ORF:  115 amino acids  =  12.4 kDa
ORF:  1031 .. 1366  =  336 bp
ORF:  111 amino acids  =  12.5 kDa
ORF:  1923 .. 2717  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  4359 .. 268  =  282 bp
ORF:  93 amino acids  =  10.4 kDa
ORF:  848 .. 1087  =  240 bp
ORF:  79 amino acids  =  8.9 kDa
ORF:  3947 .. 4273  =  327 bp
ORF:  108 amino acids  =  12.1 kDa
ORF:  4343 .. 1032  =  1062 bp
ORF:  353 amino acids  =  39.9 kDa
ORF:  633 .. 869  =  237 bp
ORF:  78 amino acids  =  8.0 kDa
ORF:  2232 .. 2486  =  255 bp
ORF:  84 amino acids  =  9.8 kDa
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Download pFN18K HaloTag T7.dna file

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