pVLT31

Broad-host-range bacterial cloning vector with the pUC18/19 MCS and a tetracycline resistance gene.
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HomePlasmidsBasic Cloning VectorspVLT31
XbaI (9615) SmaI (9606) KpnI - TspMI - XmaI (9604) Acc65I (9600) SacI (9598) Eco53kI (9596) EcoRI (9588) lac operator MauBI (9313) MluI (8856) ApaI (8653) PspOMI (8649) HpaI (8354) BsmBI - Esp3I (8241) BspQI - SapI (8048) PfoI * (7287) AcuI (5729) PasI (4860) PstI - SbfI (9631) DraIII (414) NheI (644) BmtI (648) PshAI (1131) EagI (1354) NruI (1389) BsaBI * (1888) SfiI (2479) BstZ17I (3854) pVLT31 9669 bp
XbaI  (9615)
1 site		 T C T A G A A G A T C T
SmaI  (9606)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
KpnI  (9604)
1 site		 G G T A C C C C A T G G
TspMI  (9604)
1 site		 C C C G G G G G G C C C
XmaI  (9604)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
Acc65I  (9600)
1 site		 G G T A C C C C A T G G
SacI  (9598)
1 site		 G A G C T C C T C G A G
Eco53kI  (9596)
1 site		 G A G C T C C T C G A G
EcoRI  (9588)
1 site		 G A A T T C C T T A A G
MauBI  (9313)
1 site		 C G C G C G C G G C G C G C G C
MluI  (8856)
1 site		 A C G C G T T G C G C A
ApaI  (8653)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
PspOMI  (8649)
1 site		 G G G C C C C C C G G G
HpaI  (8354)
1 site		 G T T A A C C A A T T G
BsmBI  (8241)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (8241)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
BspQI  (8048)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (8048)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PfoI  (7287)
1 site		 T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
AcuI  (5729)
1 site		 C T G A A G ( N ) 14 N N G A C T T C ( N ) 14
Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
PasI  (4860)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
PstI  (9631)
1 site		 C T G C A G G A C G T C
SbfI  (9631)
1 site		 C C T G C A G G G G A C G T C C
DraIII  (414)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
NheI  (644)
1 site		 G C T A G C C G A T C G
BmtI  (648)
1 site		 G C T A G C C G A T C G
PshAI  (1131)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
EagI  (1354)
1 site		 C G G C C G G C C G G C
NruI  (1389)
1 site		 T C G C G A A G C G C T
BsaBI  (1888)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
SfiI  (2479)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BstZ17I  (3854)
1 site		 G T A T A C C A T A T G
TcR
501 .. 1691  =  1191 bp
396 amino acids  =  41.5 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
TcR
501 .. 1691  =  1191 bp
396 amino acids  =  41.5 kDa
Product: tetracycline efflux protein
confers resistance to tetracycline
lacI
8129 .. 9211  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
8129 .. 9211  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
RSF1010 RepB
4693 .. 5664  =  972 bp
323 amino acids  =  35.9 kDa
Product: replication protein B of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepB
4693 .. 5664  =  972 bp
323 amino acids  =  35.9 kDa
Product: replication protein B of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepC
7001 .. 7852  =  852 bp
283 amino acids  =  31.1 kDa
Product: replication protein C of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepC
7001 .. 7852  =  852 bp
283 amino acids  =  31.1 kDa
Product: replication protein C of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 oriV
2631 .. 3025  =  395 bp
replication origin of the broad-host-range plasmid RSF1010; requires the RSF1010 RepA/B/C proteins for replication (Scholz et al., 1989)
RSF1010 oriV
2631 .. 3025  =  395 bp
replication origin of the broad-host-range plasmid RSF1010; requires the RSF1010 RepA/B/C proteins for replication (Scholz et al., 1989)
T4 terminators
1 .. 116  =  116 bp
T4 transcription termination signals
T4 terminators
1 .. 116  =  116 bp
T4 transcription termination signals
T4 terminators
1997 .. 2112  =  116 bp
T4 transcription termination signals
T4 terminators
1997 .. 2112  =  116 bp
T4 transcription termination signals
RSF1010 oriT
3367 .. 3454  =  88 bp
origin of transfer of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 oriT
3367 .. 3454  =  88 bp
origin of transfer of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
lacIq promoter
9212 .. 9289  =  78 bp
In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold.
lacIq promoter
9212 .. 9289  =  78 bp
In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold.
MCS
9588 .. 9644  =  57 bp
pUC18/19 multiple cloning site
MCS
9588 .. 9644  =  57 bp
pUC18/19 multiple cloning site
tac promoter
9519 .. 9547  =  29 bp
3 segments
   Segment 1:  -35  
   9519 .. 9524  =  6 bp
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
tac promoter
9519 .. 9547  =  29 bp
3 segments
   Segment 2:  
   9525 .. 9540  =  16 bp
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
tac promoter
9519 .. 9547  =  29 bp
3 segments
   Segment 3:  -10  
   9541 .. 9547  =  7 bp
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
tac promoter
9519 .. 9547  =  29 bp
3 segments
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
lac operator
9555 .. 9571  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
9555 .. 9571  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
RSF1010 RepA
6175 .. 7014  =  840 bp
279 amino acids  =  29.9 kDa
Product: replication protein A of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepA
6175 .. 7014  =  840 bp
279 amino acids  =  29.9 kDa
Product: replication protein A of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
ORF:  3535 .. 5664  =  2130 bp
ORF:  709 amino acids  =  78.0 kDa
ORF:  6175 .. 7014  =  840 bp
ORF:  279 amino acids  =  29.9 kDa
ORF:  674 .. 1057  =  384 bp
ORF:  127 amino acids  =  14.7 kDa
ORF:  1595 .. 1855  =  261 bp
ORF:  86 amino acids  =  9.7 kDa
ORF:  2831 .. 3259  =  429 bp
ORF:  142 amino acids  =  15.3 kDa
ORF:  3377 .. 3784  =  408 bp
ORF:  135 amino acids  =  14.3 kDa
ORF:  4283 .. 4696  =  414 bp
ORF:  137 amino acids  =  15.1 kDa
ORF:  5120 .. 5764  =  645 bp
ORF:  214 amino acids  =  22.8 kDa
ORF:  6902 .. 7852  =  951 bp
ORF:  316 amino acids  =  34.4 kDa
ORF:  501 .. 1691  =  1191 bp
ORF:  396 amino acids  =  41.5 kDa
ORF:  2217 .. 2624  =  408 bp
ORF:  135 amino acids  =  15.0 kDa
ORF:  3052 .. 3336  =  285 bp
ORF:  94 amino acids  =  10.9 kDa
ORF:  3406 .. 4062  =  657 bp
ORF:  218 amino acids  =  22.9 kDa
ORF:  4255 .. 4593  =  339 bp
ORF:  112 amino acids  =  12.0 kDa
ORF:  4810 .. 5403  =  594 bp
ORF:  197 amino acids  =  20.5 kDa
ORF:  6172 .. 6939  =  768 bp
ORF:  255 amino acids  =  27.0 kDa
ORF:  7342 .. 7683  =  342 bp
ORF:  113 amino acids  =  12.6 kDa
ORF:  738 .. 980  =  243 bp
ORF:  80 amino acids  =  8.0 kDa
ORF:  1038 .. 1496  =  459 bp
ORF:  152 amino acids  =  16.7 kDa
ORF:  2253 .. 2498  =  246 bp
ORF:  81 amino acids  =  9.4 kDa
ORF:  3702 .. 3932  =  231 bp
ORF:  76 amino acids  =  8.4 kDa
ORF:  4599 .. 4952  =  354 bp
ORF:  117 amino acids  =  12.6 kDa
ORF:  6426 .. 6791  =  366 bp
ORF:  121 amino acids  =  13.3 kDa
ORF:  8109 .. 8372  =  264 bp
ORF:  87 amino acids  =  8.9 kDa
ORF:  8961 .. 9278  =  318 bp
ORF:  105 amino acids  =  11.2 kDa
ORF:  854 .. 1204  =  351 bp
ORF:  116 amino acids  =  12.2 kDa
ORF:  2261 .. 2491  =  231 bp
ORF:  76 amino acids  =  8.5 kDa
ORF:  7235 .. 7573  =  339 bp
ORF:  112 amino acids  =  12.1 kDa
ORF:  8129 .. 9088  =  960 bp
ORF:  319 amino acids  =  34.1 kDa
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Individual Sequences & Maps

BlueScribe
pEASY-T1 (linearized)
pJET1.2
pSMART LCAmp
CAG promoter
pEASY-T1 Simple (linearized)
pJET1.2 blunt
pSMART LCAmp (linearized)
chicken beta-actin promoter
pEASY-T3 (linearized)
pJW168
pSMART LCKan
CLIP-tag
pEASY-T5 Zero (linearized)
pKF 18k-2
pSMART LCKan (linearized)
CMV promoter
pEX-1
pKF 19k-2
pSP64 Poly(A)
GAL1 promoter
pEX-A
pKOV
pSP72
GST
pEX-C-GST
pLATE11 (linearized)
pSP73
HaloTag
pEX-C-His
pLATE31 (linearized)
pSpark Done (linearized)
HSV TK promoter
pEX-K
pLATE51 (linearized)
pSpark I (linearized)
lac operator
pEX-N-GST
pLATE52 (linearized)
pSpark II (linearized)
lac promoter
pEX-N-His
pLEXX-AK
pSpark III (linearized)
lac UV5 promoter
pEX-N-His-GST
pLPS-B (linearized)
pSpark IV (linearized)
lacZ
pEX1
pMAL-c5E
pSpark V (linearized)
lambda
pEX2
pMAL-c5G
pSpot1
LITMUS28
pEX3
pMAL-c5X
pSpot2
LITMUS28i
pEZSeq-Amp
pMAL-c5X-His
pSpot3
LITMUS29
pEZSeq-Amp (linearized)
pMAL-c6T
pSpot4
LITMUS38
pEZSeq-Kan
pMAL-p5E
pSTBlue-1
LITMUS38i
pEZSeq-Kan (linearized)
pMAL-p5G
pSTV28
LITMUS39
pEZ BAC
pMAL-p5X
pSTV29
M13mp18
pEZ BAC (BamHI linearized)
pMCS5
pT7Blue
M13mp19
pEZ BAC (HpaI linearized)
pMiniT
pT7Blue-2
MBP
pF1A T7
pMiniT (linearized)
pT7Blue-3
pACYC177
pF1K T7
pMiniT 2.0
pT7CFE1-CFtag
pACYC184
pF25A ICE T7
pMiniT 2.0 (linearized)
pT7CFE1-CGFP-HA-His
pAN7-1
pF25K ICE T7
pMMB190
pT7CFE1-CGST-HA-His
pANT7_cGST
pF3A WG (BYDV)
pMMB206
pT7CFE1-CHA
pANT7_nHA
pF3K WG (BYDV)
pMMB207
pT7CFE1-CHis
pAUR316
pFC20A HaloTag T7 SP6
pMMB208
pT7CFE1-CMyc
pBC KS(+)
pFC20K HaloTag T7 SP6
pMMB66EH
pT7CFE1-NFtag
pBC KS(-)
pFC30A His6HaloTag T7
pMMB66HE
pT7CFE1-NHA
pBC SK(+)
pFC30K His6HaloTag T7
pMMB67EH
pT7CFE1-NHA-CHis
pBC SK(-)
pFC7A (HQ)
pMMB67HE
pT7CFE1-NHis
pBeloBAC11
pFC7K (HQ)
pNEB193
pT7CFE1-NHis-GST
pBluescript II KS(+)
pFN18A HaloTag T7
pNEB206A (linearized)
pT7CFE1-NHis-GST-CHA
pBluescript II KS(-)
pFN18K HaloTag T7
pOSIP-CH
pT7CFE1-NMyc
pBluescript II SK(+)
pFN19A HaloTag T7 SP6
pOSIP-CO
pTOP Blunt V2 (linearized)
pBluescript II SK(-)
pFN19K HaloTag T7 SP6
pOSIP-CT
pTrcHis A
pBluescript KS(+)
pFN29A His6HaloTag T7
pOSIP-KC
pTrcHis B
pBluescript KS(-)
pFN29K His6HaloTag T7
pOSIP-KH
pTrcHis C
pBluescript SK(+)
pFN2A (GST)
pOSIP-KL
pTrcHis2 A
pBluescript SK(-)
pFN2K (GST)
pOSIP-KO
pTrcHis2 B
pBR322
pFN6A (HQ)
pOSIP-KP
pTrcHis2 C
pBS KS(+)
pFN6K (HQ)
pOSIP-KT
pTV 118N
pBS KS(-)
pGEM-11Zf(+)
pOSIP-TH
pTWV228
pBS SK(+)
pGEM-11Zf(-)
pOSIP-TO
pTXB1
pBS SK(-)
pGEM-3Z
pOSIP-TT
pTYB21
pBS(+)
pGEM-3Zf(+)
pPCR-Script Amp SK(+)
pType IIs
pBS(-)
pGEM-3Zf(-)
pPCR-Script Cam SK(+)
pTZ19R
pBSK(+) Simple-Amp
pGEM-4Z
pPTR I
pTZ19U
pBSK(+) Simple-Kan
pGEM-5Zf(+)
pPTR II
pTZ57R
pcDNA3
pGEM-7Zf(+)
pRACE (linearized)
pUAST
pcDNA3.1(+)
pGEM-7Zf(-)
pRANGER-BTB-1
pUC118
pcDNA3.1(-)
pGEM-9Zf(-)
pRANGER-BTB-2
pUC119
pCold I
pGEM-T
pRANGER-BTB-3
pUC18
pCold II
pGEM-T (linearized)
pRANGER-BTB-5
pUC19
pCold III
pGEM-T Easy
pRHA-109
pUC19L
pCold IV
pGEM-T Easy (linearized)
pRHA-113
pUC57
pCold TF
pGGA
pRHA-67
pUC57-Kan
pCR-XL-2-TOPO
pGGAselect
pRSET A
pUC57-Simple
pCR-XL-2-TOPO (linearized)
PGK promoter
pRSET B
pUCmu
pDD
pGS-21a
pRSET C
pVLT31
pDNR-Dual
pH6HTC His6HaloTag T7
pSB1C3
pVLT33
pDNR-LIB
pH6HTN His6HaloTag T7
pSC101
pVLT35
pE-SUMOpro Amp
pHP45(omega)
pSEAP2-Basic
pYES1L
pE-SUMOpro Kan
pHP45(omega)-Km
pSF-Core
pZErO-2
pE-SUMOpro3 Amp
pHP45(omega)-Tc
pSMART BAC v2.0
SNAP-tag
pE-SUMOpro3 Kan
pHSG298
pSMART BAC v2.0 (BamHI linearized)
SP6 promoter
pE-SUMOstar Amp
pHSG299
pSMART BAC v2.0 (EcoRI linearized)
SV40 promoter
pE-SUMOstar Kan
pHSG396
pSMART BAC v2.0 (HindIII linearized)
T3 promoter
pEASY-Blunt (linearized)
pHSG398
pSMART FOS v2.0 (linearized)
T5 promoter
pEASY-Blunt E1 (linearized)
pHY300PLK
pSMART HCAmp
T7 promoter
pEASY-Blunt E2 (linearized)
pICOz
pSMART HCAmp (linearized)
tac promoter
pEASY-Blunt Simple (linearized)
pInvRecA
pSMART HCKan
trc promoter
pEASY-Blunt Zero (linearized)
pJAZZ-OC
pSMART HCKan (linearized)
phiX174
pEASY-Blunt3 (linearized)
pJAZZ-OK

Plasmid Sets

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I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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