pMMB208

Broad-host-range bacterial cloning vector with the pUC18/19 MCS and a chloramphenicol resistance gene. See also pMMB207.
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HomePlasmidsBasic Cloning VectorspMMB208
EcoRI (9055) SacI (9053) Eco53kI (9051) KpnI (9047) Acc65I (9043) SmaI (9041) TspMI - XmaI (9039) BamHI (9034) XbaI (9028) SalI (9022) PstI - SbfI (9020) SfcI (9016) SphI (9014) HindIII (9004) PacI (9002) lac operator tac promoter MauBI (8720) MluI (8263) ApaI (8060) PspOMI (8056) HpaI (7761) BspQI - SapI (7455) FspAI (6984) EcoNI (6834) PfoI * (6694) SgrAI (4746) XmnI (0) BsaI (148) rrnB T1 terminator rrnB T2 terminator BssSI - BssSαI (612) BspEI (1256) SnaBI (1266) SfiI (1886) EcoO109I (2438) BstZ17I (3261) pMMB208 9090 bp
EcoRI  (9055)
1 site		 G A A T T C C T T A A G
SacI  (9053)
1 site		 G A G C T C C T C G A G
Eco53kI  (9051)
1 site		 G A G C T C C T C G A G
KpnI  (9047)
1 site		 G G T A C C C C A T G G
Acc65I  (9043)
1 site		 G G T A C C C C A T G G
SmaI  (9041)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
TspMI  (9039)
1 site		 C C C G G G G G G C C C
XmaI  (9039)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
BamHI  (9034)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (9028)
1 site		 T C T A G A A G A T C T
SalI  (9022)
1 site		 G T C G A C C A G C T G
PstI  (9020)
1 site		 C T G C A G G A C G T C
SbfI  (9020)
1 site		 C C T G C A G G G G A C G T C C
SfcI  (9016)
1 site		 C T R Y A G G A Y R T C
Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
SphI  (9014)
1 site		 G C A T G C C G T A C G
HindIII  (9004)
1 site		 A A G C T T T T C G A A
PacI  (9002)
1 site		 T T A A T T A A A A T T A A T T
MauBI  (8720)
1 site		 C G C G C G C G G C G C G C G C
MluI  (8263)
1 site		 A C G C G T T G C G C A
ApaI  (8060)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
PspOMI  (8056)
1 site		 G G G C C C C C C G G G
HpaI  (7761)
1 site		 G T T A A C C A A T T G
BspQI  (7455)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (7455)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
FspAI  (6984)
1 site		 R T G C G C A Y Y A C G C G T R
EcoNI  (6834)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PfoI  (6694)
1 site		 T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
SgrAI  (4746)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
XmnI  (0)
1 site		 G A A N N N N T T C C T T N N N N A A G
BsaI  (148)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BssSI  (612)
1 site		 C A C G A G G T G C T C
BssSαI  (612)
1 site		 C A C G A G G T G C T C
BspEI  (1256)
1 site		 T C C G G A A G G C C T
SnaBI  (1266)
1 site		 T A C G T A A T G C A T
SfiI  (1886)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
EcoO109I  (2438)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
BstZ17I  (3261)
1 site		 G T A T A C C A T A T G
lacI
7536 .. 8618  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lacI
7536 .. 8618  =  1083 bp
360 amino acids  =  38.6 kDa
Product: lac repressor
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
RSF1010 RepB
4100 .. 5071  =  972 bp
323 amino acids  =  35.9 kDa
Product: replication protein B of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepB
4100 .. 5071  =  972 bp
323 amino acids  =  35.9 kDa
Product: replication protein B of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepC
6408 .. 7259  =  852 bp
283 amino acids  =  31.1 kDa
Product: replication protein C of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepC
6408 .. 7259  =  852 bp
283 amino acids  =  31.1 kDa
Product: replication protein C of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
CmR
1047 .. 1706  =  660 bp
219 amino acids  =  25.6 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
1047 .. 1706  =  660 bp
219 amino acids  =  25.6 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
RSF1010 oriV
2038 .. 2432  =  395 bp
replication origin of the broad-host-range plasmid RSF1010; requires the RSF1010 RepA/B/C proteins for replication (Scholz et al., 1989)
RSF1010 oriV
2038 .. 2432  =  395 bp
replication origin of the broad-host-range plasmid RSF1010; requires the RSF1010 RepA/B/C proteins for replication (Scholz et al., 1989)
cat promoter
944 .. 1046  =  103 bp
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
cat promoter
944 .. 1046  =  103 bp
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
RSF1010 oriT
2774 .. 2861  =  88 bp
origin of transfer of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 oriT
2774 .. 2861  =  88 bp
origin of transfer of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
rrnB T1 terminator
178 .. 264  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
178 .. 264  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
lacIq promoter
8619 .. 8696  =  78 bp
In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold.
lacIq promoter
8619 .. 8696  =  78 bp
In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold.
MCS
9004 .. 9060  =  57 bp
pUC18/19 multiple cloning site
MCS
9004 .. 9060  =  57 bp
pUC18/19 multiple cloning site
tac promoter
8926 .. 8954  =  29 bp
3 segments
   Segment 1:  -35  
   8926 .. 8931  =  6 bp
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
tac promoter
8926 .. 8954  =  29 bp
3 segments
   Segment 2:  
   8932 .. 8947  =  16 bp
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
tac promoter
8926 .. 8954  =  29 bp
3 segments
   Segment 3:  -10  
   8948 .. 8954  =  7 bp
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
tac promoter
8926 .. 8954  =  29 bp
3 segments
strong E. coli promoter; hybrid between the trp and lac UV5 promoters
rrnB T2 terminator
356 .. 383  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
rrnB T2 terminator
356 .. 383  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
lac operator
8962 .. 8978  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
8962 .. 8978  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
RSF1010 RepA
5582 .. 6421  =  840 bp
279 amino acids  =  29.9 kDa
Product: replication protein A of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
RSF1010 RepA
5582 .. 6421  =  840 bp
279 amino acids  =  29.9 kDa
Product: replication protein A of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)
ORF:  1624 .. 2031  =  408 bp
ORF:  135 amino acids  =  15.0 kDa
ORF:  494 .. 727  =  234 bp
ORF:  77 amino acids  =  8.9 kDa
ORF:  2942 .. 5071  =  2130 bp
ORF:  709 amino acids  =  78.0 kDa
ORF:  5582 .. 6421  =  840 bp
ORF:  279 amino acids  =  29.9 kDa
ORF:  1047 .. 1706  =  660 bp
ORF:  219 amino acids  =  25.6 kDa
ORF:  2238 .. 2666  =  429 bp
ORF:  142 amino acids  =  15.3 kDa
ORF:  2784 .. 3191  =  408 bp
ORF:  135 amino acids  =  14.3 kDa
ORF:  3690 .. 4103  =  414 bp
ORF:  137 amino acids  =  15.1 kDa
ORF:  4527 .. 5171  =  645 bp
ORF:  214 amino acids  =  22.8 kDa
ORF:  6309 .. 7259  =  951 bp
ORF:  316 amino acids  =  34.4 kDa
ORF:  19 .. 345  =  327 bp
ORF:  108 amino acids  =  12.1 kDa
ORF:  1660 .. 1905  =  246 bp
ORF:  81 amino acids  =  9.4 kDa
ORF:  3109 .. 3339  =  231 bp
ORF:  76 amino acids  =  8.4 kDa
ORF:  4006 .. 4359  =  354 bp
ORF:  117 amino acids  =  12.6 kDa
ORF:  5833 .. 6198  =  366 bp
ORF:  121 amino acids  =  13.3 kDa
ORF:  7516 .. 7779  =  264 bp
ORF:  87 amino acids  =  8.9 kDa
ORF:  8368 .. 8685  =  318 bp
ORF:  105 amino acids  =  11.2 kDa
ORF:  1668 .. 1898  =  231 bp
ORF:  76 amino acids  =  8.5 kDa
ORF:  6642 .. 6980  =  339 bp
ORF:  112 amino acids  =  12.1 kDa
ORF:  7536 .. 8495  =  960 bp
ORF:  319 amino acids  =  34.1 kDa
ORF:  425 .. 793  =  369 bp
ORF:  122 amino acids  =  14.1 kDa
ORF:  2459 .. 2743  =  285 bp
ORF:  94 amino acids  =  10.9 kDa
ORF:  2813 .. 3469  =  657 bp
ORF:  218 amino acids  =  22.9 kDa
ORF:  3662 .. 4000  =  339 bp
ORF:  112 amino acids  =  12.0 kDa
ORF:  4217 .. 4810  =  594 bp
ORF:  197 amino acids  =  20.5 kDa
ORF:  5579 .. 6346  =  768 bp
ORF:  255 amino acids  =  27.0 kDa
ORF:  6749 .. 7090  =  342 bp
ORF:  113 amino acids  =  12.6 kDa
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Individual Sequences & Maps

BlueScribe
pEASY-T1 (linearized)
pJET1.2
pSMART LCAmp
CAG promoter
pEASY-T1 Simple (linearized)
pJET1.2 blunt
pSMART LCAmp (linearized)
chicken beta-actin promoter
pEASY-T3 (linearized)
pJW168
pSMART LCKan
CLIP-tag
pEASY-T5 Zero (linearized)
pKF 18k-2
pSMART LCKan (linearized)
CMV promoter
pEX-1
pKF 19k-2
pSP64 Poly(A)
GAL1 promoter
pEX-A
pKOV
pSP72
GST
pEX-C-GST
pLATE11 (linearized)
pSP73
HaloTag
pEX-C-His
pLATE31 (linearized)
pSpark Done (linearized)
HSV TK promoter
pEX-K
pLATE51 (linearized)
pSpark I (linearized)
lac operator
pEX-N-GST
pLATE52 (linearized)
pSpark II (linearized)
lac promoter
pEX-N-His
pLEXX-AK
pSpark III (linearized)
lac UV5 promoter
pEX-N-His-GST
pLPS-B (linearized)
pSpark IV (linearized)
lacZ
pEX1
pMAL-c5E
pSpark V (linearized)
lambda
pEX2
pMAL-c5G
pSpot1
LITMUS28
pEX3
pMAL-c5X
pSpot2
LITMUS28i
pEZSeq-Amp
pMAL-c5X-His
pSpot3
LITMUS29
pEZSeq-Amp (linearized)
pMAL-c6T
pSpot4
LITMUS38
pEZSeq-Kan
pMAL-p5E
pSTBlue-1
LITMUS38i
pEZSeq-Kan (linearized)
pMAL-p5G
pSTV28
LITMUS39
pEZ BAC
pMAL-p5X
pSTV29
M13mp18
pEZ BAC (BamHI linearized)
pMCS5
pT7Blue
M13mp19
pEZ BAC (HpaI linearized)
pMiniT
pT7Blue-2
MBP
pF1A T7
pMiniT (linearized)
pT7Blue-3
pACYC177
pF1K T7
pMiniT 2.0
pT7CFE1-CFtag
pACYC184
pF25A ICE T7
pMiniT 2.0 (linearized)
pT7CFE1-CGFP-HA-His
pAN7-1
pF25K ICE T7
pMMB190
pT7CFE1-CGST-HA-His
pANT7_cGST
pF3A WG (BYDV)
pMMB206
pT7CFE1-CHA
pANT7_nHA
pF3K WG (BYDV)
pMMB207
pT7CFE1-CHis
pAUR316
pFC20A HaloTag T7 SP6
pMMB208
pT7CFE1-CMyc
pBC KS(+)
pFC20K HaloTag T7 SP6
pMMB66EH
pT7CFE1-NFtag
pBC KS(-)
pFC30A His6HaloTag T7
pMMB66HE
pT7CFE1-NHA
pBC SK(+)
pFC30K His6HaloTag T7
pMMB67EH
pT7CFE1-NHA-CHis
pBC SK(-)
pFC7A (HQ)
pMMB67HE
pT7CFE1-NHis
pBeloBAC11
pFC7K (HQ)
pNEB193
pT7CFE1-NHis-GST
pBluescript II KS(+)
pFN18A HaloTag T7
pNEB206A (linearized)
pT7CFE1-NHis-GST-CHA
pBluescript II KS(-)
pFN18K HaloTag T7
pOSIP-CH
pT7CFE1-NMyc
pBluescript II SK(+)
pFN19A HaloTag T7 SP6
pOSIP-CO
pTOP Blunt V2 (linearized)
pBluescript II SK(-)
pFN19K HaloTag T7 SP6
pOSIP-CT
pTrcHis A
pBluescript KS(+)
pFN29A His6HaloTag T7
pOSIP-KC
pTrcHis B
pBluescript KS(-)
pFN29K His6HaloTag T7
pOSIP-KH
pTrcHis C
pBluescript SK(+)
pFN2A (GST)
pOSIP-KL
pTrcHis2 A
pBluescript SK(-)
pFN2K (GST)
pOSIP-KO
pTrcHis2 B
pBR322
pFN6A (HQ)
pOSIP-KP
pTrcHis2 C
pBS KS(+)
pFN6K (HQ)
pOSIP-KT
pTV 118N
pBS KS(-)
pGEM-11Zf(+)
pOSIP-TH
pTWV228
pBS SK(+)
pGEM-11Zf(-)
pOSIP-TO
pTXB1
pBS SK(-)
pGEM-3Z
pOSIP-TT
pTYB21
pBS(+)
pGEM-3Zf(+)
pPCR-Script Amp SK(+)
pType IIs
pBS(-)
pGEM-3Zf(-)
pPCR-Script Cam SK(+)
pTZ19R
pBSK(+) Simple-Amp
pGEM-4Z
pPTR I
pTZ19U
pBSK(+) Simple-Kan
pGEM-5Zf(+)
pPTR II
pTZ57R
pcDNA3
pGEM-7Zf(+)
pRACE (linearized)
pUAST
pcDNA3.1(+)
pGEM-7Zf(-)
pRANGER-BTB-1
pUC118
pcDNA3.1(-)
pGEM-9Zf(-)
pRANGER-BTB-2
pUC119
pCold I
pGEM-T
pRANGER-BTB-3
pUC18
pCold II
pGEM-T (linearized)
pRANGER-BTB-5
pUC19
pCold III
pGEM-T Easy
pRHA-109
pUC19L
pCold IV
pGEM-T Easy (linearized)
pRHA-113
pUC57
pCold TF
pGGA
pRHA-67
pUC57-Kan
pCR-XL-2-TOPO
pGGAselect
pRSET A
pUC57-Simple
pCR-XL-2-TOPO (linearized)
PGK promoter
pRSET B
pUCmu
pDD
pGS-21a
pRSET C
pVLT31
pDNR-Dual
pH6HTC His6HaloTag T7
pSB1C3
pVLT33
pDNR-LIB
pH6HTN His6HaloTag T7
pSC101
pVLT35
pE-SUMOpro Amp
pHP45(omega)
pSEAP2-Basic
pYES1L
pE-SUMOpro Kan
pHP45(omega)-Km
pSF-Core
pZErO-2
pE-SUMOpro3 Amp
pHP45(omega)-Tc
pSMART BAC v2.0
SNAP-tag
pE-SUMOpro3 Kan
pHSG298
pSMART BAC v2.0 (BamHI linearized)
SP6 promoter
pE-SUMOstar Amp
pHSG299
pSMART BAC v2.0 (EcoRI linearized)
SV40 promoter
pE-SUMOstar Kan
pHSG396
pSMART BAC v2.0 (HindIII linearized)
T3 promoter
pEASY-Blunt (linearized)
pHSG398
pSMART FOS v2.0 (linearized)
T5 promoter
pEASY-Blunt E1 (linearized)
pHY300PLK
pSMART HCAmp
T7 promoter
pEASY-Blunt E2 (linearized)
pICOz
pSMART HCAmp (linearized)
tac promoter
pEASY-Blunt Simple (linearized)
pInvRecA
pSMART HCKan
trc promoter
pEASY-Blunt Zero (linearized)
pJAZZ-OC
pSMART HCKan (linearized)
phiX174
pEASY-Blunt3 (linearized)
pJAZZ-OK

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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