pF1A T7

Flexi® vector with an ampicillin resistance marker, for bacterial or in vitro expression of an untagged protein.

Sequence Author: Promega

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HomePlasmidsBasic Cloning VectorspF1A T7
T7 promoter BglII (3007) MluI (2888) cer region BspEI (2675) DrdI (2019) PciI (1911) AgeI (1843) BstBI (1816) AhdI (1741) SpeI (67) AsiSI - PvuI - SgfI (104) BtgI - NcoI (107) DraI - PmeI (449) EcoRI (454) Eco53kI (462) BanII - SacI (464) Acc65I (466) AvaI - BmeT110I - BsoBI - KpnI - TspMI - XmaI (470) SmaI (472) BamHI (475) XbaI (481) SalI (487) AccI (488) HincII (489) PstI - SbfI (497) SphI (503) BlpI (562) EcoO109I (589) BsaAI - SnaBI (803) SspI (936) EarI (942) XmnI (1141) FspI (1518) BglI (1623) pF1A T7 3455 bp
BglII  (3007)
1 site		 A G A T C T T C T A G A
MluI  (2888)
1 site		 A C G C G T T G C G C A
BspEI  (2675)
1 site		 T C C G G A A G G C C T
DrdI  (2019)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
PciI  (1911)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
AgeI  (1843)
1 site		 A C C G G T T G G C C A
BstBI  (1816)
1 site		 T T C G A A A A G C T T
AhdI  (1741)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SpeI  (67)
1 site		 A C T A G T T G A T C A
AsiSI  (104)
1 site		 G C G A T C G C C G C T A G C G
PvuI  (104)
1 site		 C G A T C G G C T A G C
SgfI  (104)
1 site		 G C G A T C G C C G C T A G C G
BtgI  (107)
1 site		 C C R Y G G G G Y R C C
Sticky ends from different BtgI sites may not be compatible.
NcoI  (107)
1 site		 C C A T G G G G T A C C
DraI  (449)
1 site		 T T T A A A A A A T T T
PmeI  (449)
1 site		 G T T T A A A C C A A A T T T G
EcoRI  (454)
1 site		 G A A T T C C T T A A G
Eco53kI  (462)
1 site		 G A G C T C C T C G A G
BanII  (464)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
SacI  (464)
1 site		 G A G C T C C T C G A G
Acc65I  (466)
1 site		 G G T A C C C C A T G G
AvaI  (470)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BmeT110I  (470)
1 site		 C Y C G R G G R G C Y C
BsoBI  (470)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
KpnI  (470)
1 site		 G G T A C C C C A T G G
TspMI  (470)
1 site		 C C C G G G G G G C C C
XmaI  (470)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (472)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
BamHI  (475)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (481)
1 site		 T C T A G A A G A T C T
SalI  (487)
1 site		 G T C G A C C A G C T G
AccI  (488)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
HincII  (489)
1 site		 G T Y R A C C A R Y T G
PstI  (497)
1 site		 C T G C A G G A C G T C
SbfI  (497)
1 site		 C C T G C A G G G G A C G T C C
SphI  (503)
1 site		 G C A T G C C G T A C G
BlpI  (562)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
EcoO109I  (589)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
BsaAI  (803)
1 site		 Y A C G T R R T G C A Y
SnaBI  (803)
1 site		 T A C G T A A T G C A T
SspI  (936)
1 site		 A A T A T T T T A T A A
EarI  (942)
1 site		 C T C T T C N G A G A A G N N N N
Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
XmnI  (1141)
1 site		 G A A N N N N T T C C T T N N N N A A G
FspI  (1518)
1 site		 T G C G C A A C G C G T
BglI  (1623)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
AmpR
954 .. 1814  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   954 .. 1022  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
954 .. 1814  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1023 .. 1814  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
954 .. 1814  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1972 .. 2560  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1972 .. 2560  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
barnase
109 .. 444  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
barnase
109 .. 444  =  336 bp
111 amino acids  =  12.5 kDa
Product: ribonuclease from Bacillus amyloliquefaciens
The barnase gene is lethal in standard bacterial transformation strains.
cer region
2676 .. 2959  =  284 bp
ColE1-derived recombination site that helps to maintain plasmids as monomers
cer region
2676 .. 2959  =  284 bp
ColE1-derived recombination site that helps to maintain plasmids as monomers
AmpR promoter
849 .. 953  =  105 bp
AmpR promoter
849 .. 953  =  105 bp
rrnB T1 terminator
3189 .. 3275  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
3189 .. 3275  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
T7 terminator
573 .. 620  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
573 .. 620  =  48 bp
transcription terminator for bacteriophage T7 RNA polymerase
rrnB T2 terminator
3367 .. 3394  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
rrnB T2 terminator
3367 .. 3394  =  28 bp
transcription terminator T2 from the E. coli rrnB gene
lac operator
40 .. 64  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
40 .. 64  =  25 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
T7 promoter
21 .. 39  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
21 .. 39  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  109 .. 444  =  336 bp
ORF:  111 amino acids  =  12.5 kDa
ORF:  2921 .. 3268  =  348 bp
ORF:  115 amino acids  =  12.4 kDa
ORF:  954 .. 1814  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3030 .. 3356  =  327 bp
ORF:  108 amino acids  =  12.1 kDa
ORF:  1418 .. 1684  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
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Download pF1A T7.dna file

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Individual Sequences & Maps

BlueScribe
pEASY-T1 (linearized)
pJET1.2
pSMART LCAmp
CAG promoter
pEASY-T1 Simple (linearized)
pJET1.2 blunt
pSMART LCAmp (linearized)
chicken beta-actin promoter
pEASY-T3 (linearized)
pJW168
pSMART LCKan
CLIP-tag
pEASY-T5 Zero (linearized)
pKF 18k-2
pSMART LCKan (linearized)
CMV promoter
pEX-1
pKF 19k-2
pSP64 Poly(A)
GAL1 promoter
pEX-A
pKOV
pSP72
GST
pEX-C-GST
pLATE11 (linearized)
pSP73
HaloTag
pEX-C-His
pLATE31 (linearized)
pSpark Done (linearized)
HSV TK promoter
pEX-K
pLATE51 (linearized)
pSpark I (linearized)
lac operator
pEX-N-GST
pLATE52 (linearized)
pSpark II (linearized)
lac promoter
pEX-N-His
pLEXX-AK
pSpark III (linearized)
lac UV5 promoter
pEX-N-His-GST
pLPS-B (linearized)
pSpark IV (linearized)
lacZ
pEX1
pMAL-c5E
pSpark V (linearized)
lambda
pEX2
pMAL-c5G
pSpot1
LITMUS28
pEX3
pMAL-c5X
pSpot2
LITMUS28i
pEZSeq-Amp
pMAL-c5X-His
pSpot3
LITMUS29
pEZSeq-Amp (linearized)
pMAL-c6T
pSpot4
LITMUS38
pEZSeq-Kan
pMAL-p5E
pSTBlue-1
LITMUS38i
pEZSeq-Kan (linearized)
pMAL-p5G
pSTV28
LITMUS39
pEZ BAC
pMAL-p5X
pSTV29
M13mp18
pEZ BAC (BamHI linearized)
pMCS5
pT7Blue
M13mp19
pEZ BAC (HpaI linearized)
pMiniT
pT7Blue-2
MBP
pF1A T7
pMiniT (linearized)
pT7Blue-3
pACYC177
pF1K T7
pMiniT 2.0
pT7CFE1-CFtag
pACYC184
pF25A ICE T7
pMiniT 2.0 (linearized)
pT7CFE1-CGFP-HA-His
pAN7-1
pF25K ICE T7
pMMB190
pT7CFE1-CGST-HA-His
pANT7_cGST
pF3A WG (BYDV)
pMMB206
pT7CFE1-CHA
pANT7_nHA
pF3K WG (BYDV)
pMMB207
pT7CFE1-CHis
pAUR316
pFC20A HaloTag T7 SP6
pMMB208
pT7CFE1-CMyc
pBC KS(+)
pFC20K HaloTag T7 SP6
pMMB66EH
pT7CFE1-NFtag
pBC KS(-)
pFC30A His6HaloTag T7
pMMB66HE
pT7CFE1-NHA
pBC SK(+)
pFC30K His6HaloTag T7
pMMB67EH
pT7CFE1-NHA-CHis
pBC SK(-)
pFC7A (HQ)
pMMB67HE
pT7CFE1-NHis
pBeloBAC11
pFC7K (HQ)
pNEB193
pT7CFE1-NHis-GST
pBluescript II KS(+)
pFN18A HaloTag T7
pNEB206A (linearized)
pT7CFE1-NHis-GST-CHA
pBluescript II KS(-)
pFN18K HaloTag T7
pOSIP-CH
pT7CFE1-NMyc
pBluescript II SK(+)
pFN19A HaloTag T7 SP6
pOSIP-CO
pTOP Blunt V2 (linearized)
pBluescript II SK(-)
pFN19K HaloTag T7 SP6
pOSIP-CT
pTrcHis A
pBluescript KS(+)
pFN29A His6HaloTag T7
pOSIP-KC
pTrcHis B
pBluescript KS(-)
pFN29K His6HaloTag T7
pOSIP-KH
pTrcHis C
pBluescript SK(+)
pFN2A (GST)
pOSIP-KL
pTrcHis2 A
pBluescript SK(-)
pFN2K (GST)
pOSIP-KO
pTrcHis2 B
pBR322
pFN6A (HQ)
pOSIP-KP
pTrcHis2 C
pBS KS(+)
pFN6K (HQ)
pOSIP-KT
pTV 118N
pBS KS(-)
pGEM-11Zf(+)
pOSIP-TH
pTWV228
pBS SK(+)
pGEM-11Zf(-)
pOSIP-TO
pTXB1
pBS SK(-)
pGEM-3Z
pOSIP-TT
pTYB21
pBS(+)
pGEM-3Zf(+)
pPCR-Script Amp SK(+)
pType IIs
pBS(-)
pGEM-3Zf(-)
pPCR-Script Cam SK(+)
pTZ19R
pBSK(+) Simple-Amp
pGEM-4Z
pPTR I
pTZ19U
pBSK(+) Simple-Kan
pGEM-5Zf(+)
pPTR II
pTZ57R
pcDNA3
pGEM-7Zf(+)
pRACE (linearized)
pUAST
pcDNA3.1(+)
pGEM-7Zf(-)
pRANGER-BTB-1
pUC118
pcDNA3.1(-)
pGEM-9Zf(-)
pRANGER-BTB-2
pUC119
pCold I
pGEM-T
pRANGER-BTB-3
pUC18
pCold II
pGEM-T (linearized)
pRANGER-BTB-5
pUC19
pCold III
pGEM-T Easy
pRHA-109
pUC19L
pCold IV
pGEM-T Easy (linearized)
pRHA-113
pUC57
pCold TF
pGGA
pRHA-67
pUC57-Kan
pCR-XL-2-TOPO
pGGAselect
pRSET A
pUC57-Simple
pCR-XL-2-TOPO (linearized)
PGK promoter
pRSET B
pUCmu
pDD
pGS-21a
pRSET C
pVLT31
pDNR-Dual
pH6HTC His6HaloTag T7
pSB1C3
pVLT33
pDNR-LIB
pH6HTN His6HaloTag T7
pSC101
pVLT35
pE-SUMOpro Amp
pHP45(omega)
pSEAP2-Basic
pYES1L
pE-SUMOpro Kan
pHP45(omega)-Km
pSF-Core
pZErO-2
pE-SUMOpro3 Amp
pHP45(omega)-Tc
pSMART BAC v2.0
SNAP-tag
pE-SUMOpro3 Kan
pHSG298
pSMART BAC v2.0 (BamHI linearized)
SP6 promoter
pE-SUMOstar Amp
pHSG299
pSMART BAC v2.0 (EcoRI linearized)
SV40 promoter
pE-SUMOstar Kan
pHSG396
pSMART BAC v2.0 (HindIII linearized)
T3 promoter
pEASY-Blunt (linearized)
pHSG398
pSMART FOS v2.0 (linearized)
T5 promoter
pEASY-Blunt E1 (linearized)
pHY300PLK
pSMART HCAmp
T7 promoter
pEASY-Blunt E2 (linearized)
pICOz
pSMART HCAmp (linearized)
tac promoter
pEASY-Blunt Simple (linearized)
pInvRecA
pSMART HCKan
trc promoter
pEASY-Blunt Zero (linearized)
pJAZZ-OC
pSMART HCKan (linearized)
phiX174
pEASY-Blunt3 (linearized)
pJAZZ-OK

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