pCMV-Gaussia Luc

Control vector for constitutive high-level expression of secreted Gaussia luciferase under control of the CMV promoter.

Sequence Author: Thermo Fisher (Pierce)

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HomePlasmidsLuciferase VectorspCMV-Gaussia Luc
PaqCI (5453) EcoRV (5159) ApaI (4819) PspOMI (4815) AflII (4806) DrdI (4473) MauBI (3877) ScaI (3360) FspI (3102) AfeI (2930) BstZ17I (2765) SpeI (1) NdeI (236) SnaBI (342) Eco53kI (568) SacI (570) BamHI (634) BsaBI * (677) BbsI (723) NotI (1206) BseRI (1771) AvrII (1775) TspMI - XmaI (1796) SmaI (1798) BclI * (1826) PflFI - Tth111I (1946) BsiWI (1960) RsrII (2020) BstEII (2038) EarI (2063) BsmBI - Esp3I (2296) DraIII (2475) NgoMIV (2576) NaeI (2578) BsmI (2713) pCMV-Gaussia Luc 5483 bp
PaqCI  (5453)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
EcoRV  (5159)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
ApaI  (4819)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
PspOMI  (4815)
1 site		 G G G C C C C C C G G G
AflII  (4806)
1 site		 C T T A A G G A A T T C
DrdI  (4473)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
MauBI  (3877)
1 site		 C G C G C G C G G C G C G C G C
ScaI  (3360)
1 site		 A G T A C T T C A T G A
FspI  (3102)
1 site		 T G C G C A A C G C G T
AfeI  (2930)
1 site		 A G C G C T T C G C G A
BstZ17I  (2765)
1 site		 G T A T A C C A T A T G
SpeI  (1)
1 site		 A C T A G T T G A T C A
NdeI  (236)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (342)
1 site		 T A C G T A A T G C A T
Eco53kI  (568)
1 site		 G A G C T C C T C G A G
SacI  (570)
1 site		 G A G C T C C T C G A G
BamHI  (634)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BsaBI  (677)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BbsI  (723)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
NotI  (1206)
1 site		 G C G G C C G C C G C C G G C G
BseRI  (1771)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
AvrII  (1775)
1 site		 C C T A G G G G A T C C
TspMI  (1796)
1 site		 C C C G G G G G G C C C
XmaI  (1796)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (1798)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
BclI  (1826)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
PflFI  (1946)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1946)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsiWI  (1960)
1 site		 C G T A C G G C A T G C
BsiWI is typically used at 55°C, but is 50% active at 37°C.
RsrII  (2020)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BstEII  (2038)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
EarI  (2063)
1 site		 C T C T T C N G A G A A G N N N N
Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
BsmBI  (2296)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (2296)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
DraIII  (2475)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
NgoMIV  (2576)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (2578)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
BsmI  (2713)
1 site		 G A A T G C N C T T A C G N
Sticky ends from different BsmI sites may not be compatible.
AmpR
2807 .. 3667  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2807 .. 3598  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2807 .. 3667  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3599 .. 3667  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2807 .. 3667  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
PuroR
1904 .. 2503  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
1904 .. 2503  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
ori
3931 .. 4519  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3931 .. 4519  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
hGLuc
647 .. 1204  =  558 bp
185 amino acids  =  19.9 kDa
Product: secreted Gaussia luciferase
human codon-optimized
hGLuc
647 .. 1204  =  558 bp
185 amino acids  =  19.9 kDa
Product: secreted Gaussia luciferase
human codon-optimized
SV40 promoter
1461 .. 1790  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
1461 .. 1790  =  330 bp
SV40 enhancer and early promoter
CMV enhancer
63 .. 366  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
63 .. 366  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
367 .. 570  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
367 .. 570  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
2633 .. 2754  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2633 .. 2754  =  122 bp
SV40 polyadenylation signal
bGH poly(A) signal
1227 .. 1338  =  112 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
1227 .. 1338  =  112 bp
bovine growth hormone polyadenylation signal
pause site
5392 .. 5483  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
pause site
5392 .. 5483  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
EM7 promoter
1838 .. 1885  =  48 bp
synthetic bacterial promoter
EM7 promoter
1838 .. 1885  =  48 bp
synthetic bacterial promoter
T7 promoter
615 .. 633  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
615 .. 633  =  19 bp
promoter for bacteriophage T7 RNA polymerase
lac operator
5261 .. 5277  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
5261 .. 5277  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
SV40 ori
1641 .. 1776  =  136 bp
SV40 origin of replication
SV40 ori
1641 .. 1776  =  136 bp
SV40 origin of replication
ORF:  526 .. 774  =  249 bp
ORF:  82 amino acids  =  9.7 kDa
ORF:  647 .. 1204  =  558 bp
ORF:  185 amino acids  =  19.9 kDa
ORF:  1904 .. 2503  =  600 bp
ORF:  199 amino acids  =  21.5 kDa
ORF:  2937 .. 3203  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  2064 .. 2390  =  327 bp
ORF:  108 amino acids  =  12.0 kDa
ORF:  2807 .. 3667  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  577 .. 915  =  339 bp
ORF:  112 amino acids  =  12.0 kDa
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Individual Sequences & Maps

pBI-CMV5
pGL4.22[luc2CP Puro]
pGL4.81[hRlucCP Neo]
pRL-null
pBIND-ER(alpha)
pGL4.23[luc2 minP]
pGL4.82[hRluc Puro]
pRL-SV40
pBIND-GR
pGL4.24[luc2P minP]
pGL4.83[hRlucP Puro]
pRL-TK
pCBG68-Basic
pGL4.25[luc2CP minP]
pGL4.84[hRlucCP Puro]
pSELECT-zeo-Lucia
pCBG68-Control
pGL4.26[luc2 minP Hygro]
pGLuc Mini-TK 2
pSF-CMV-EMCV-FLuc
pCBG99-Basic
pGL4.27[luc2P minP Hygro]
pGLuc-Basic 2
pSF-CMV-EMCV-RLuc
pCBG99-Control
pGL4.28[luc2CP minP Hygro]
phRG-B
pSF-CMV-FLuc
pCBR-Basic
pGL4.29[luc2P CRE Hygro]
phRG-TK
pSF-CMV-FMDV-FLuc
pCBR-Control
pGL4.30[luc2P NFAT-RE Hygro]
phRL-CMV
pSF-CMV-FMDV-RLuc
pCLuc Mini-TK 2
pGL4.31[luc2P GAL4UAS Hygro]
phRL-null
pSF-CMV-PGK-FLuc
pCLuc-Basic 2
pGL4.32[luc2P NF-kB-RE Hygro]
phRL-SV40
pSF-CMV-PGK-RLuc
pCMV-CLuc 2
pGL4.33[luc2P SRE Hygro]
phRL-TK
pSF-CMV-RLuc
pCMV-Cypridina Luc
pGL4.34[luc2P SRF-RE Hygro]
phRL-TK(Int-)
pSF-CMV-RSV-FLuc AscI
pCMV-Gaussia Luc
pGL4.35[luc2P 9XGAL4UAS Hygro]
pLightSwitch_Prom
pSF-CMV-RSV-RLuc AscI
pCMV-Gaussia-Dura Luc
pGL4.36[luc2P MMTV Hygro]
pLVX-MetLuc Control
pSF-CMV-Ub-FLuc AscI
pCMV-GLuc 2
pGL4.37[luc2P ARE Hygro]
pLVX-MetLuc Reporter
pSF-CMV-Ub-RLuc AscI
pCMV-Green Renilla Luc
pGL4.38[luc2P p53 RE Hygro]
pMCS-Cypridina Luc
pSF-CMVe-FLuc
pCMV-Red Firefly Luc
pGL4.39[luc2P ATF6 RE Hygro]
pMCS-Gaussia Luc
pSF-CMVe-RLuc
pCRE-MetLuc2-Reporter
pGL4.40[luc2P MRE Hygro]
pMCS-Gaussia-Dura Luc
pSF-MinProm-FLuc
pFN26A (BIND) hRluc-neo Flexi Vector
pGL4.41[luc2P HSE Hygro]
pMCS-Green Renilla Luc
pSF-MinProm-RLuc
pGEM-luc
pGL4.42[luc2P HRE Hygro]
pMCS-Red Firefly Luc
pSF-pA-CMVe-FLuc
pGL2-Basic
pGL4.43[luc2P XRE Hygro]
pMetLuc-Mem Control
pSF-pA-CMVe-RLuc
pGL2-Control
pGL4.44[luc2P AP1 RE Hygro]
pMetLuc-Mem Reporter
pSF-pA-MinProm-FLuc
pGL2-Enhancer
pGL4.45[luc2P ISRE Hygro]
pMetLuc2-Control
pSF-pA-MinProm-RLuc
pGL2-Promoter
pGL4.47[luc2P SIE Hygro]
pMetLuc2-Reporter
pSF-pA-PromMCS-FLuc
pGL3-Basic
pGL4.48[luc2P SBE Hygro]
pmirGLO
pSF-pA-PromMCS-RLuc
pGL3-Control
pGL4.49[luc2P TCF-LEF RE Hygro]
pMirTarget
pSF-PromMCS-FLuc
pGL3-Enhancer
pGL4.50[luc2 CMV Hygro]
pNFkB-MetLuc2-Reporter
pSF-PromMCS-RLuc
pGL3-Promoter
pGL4.51[luc2 CMV Neo]
pNL1.1.CMV[Nluc CMV]
pSF-RecA-FLuc
pGL4.10[luc2]
pGL4.52[luc2P STAT5 RE Hygro]
pNL1.1[Nluc]
pSP-luc+NF
pGL4.11[luc2P]
pGL4.70[hRluc]
pNL1.2[NlucP]
pSV40-CLuc
pGL4.12[luc2CP]
pGL4.71[hRlucP]
pNL1.3.CMV[secNluc CMV]
pSV40-GLuc
pGL4.13[luc2 SV40]
pGL4.72[hRlucCP]
pNL1.3[secNluc]
pTK-CLuc
pGL4.14[luc2 Hygro]
pGL4.73[hRluc SV40]
pNL2.1[Nluc Hygro]
pTK-Cypridina Luc
pGL4.15[luc2P Hygro]
pGL4.74[hRluc TK]
pNL2.2[NlucP Hygro]
pTK-Gaussia Luc
pGL4.16[luc2CP Hygro]
pGL4.75[hRluc CMV]
pNL2.3[secNluc Hygro]
pTK-Gaussia-Dura Luc
pGL4.17[luc2 Neo]
pGL4.76[hRluc Hygro]
pNL3.1[Nluc minP]
pTK-GLuc
pGL4.18[luc2P Neo]
pGL4.77[hRlucP Hygro]
pNL3.2.NF-kB-RE[NlucP NF-kB-RE Hygro]
pTK-Green Renilla Luc
pGL4.19[luc2CP Neo]
pGL4.78[hRlucCP Hygro]
pNL3.2[NlucP minP]
pTK-Red Firefly Luc
pGL4.20[luc2 Puro]
pGL4.79[hRluc Neo]
pNL3.3[secNluc minP]
pXPG
pGL4.21[luc2P Puro]
pGL4.80[hRlucP Neo]
pRL-CMV

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