pRL-null

Promoterless mammalian vector encoding wild-type Renilla luciferase.

Sequence Author: Promega

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HomePlasmidsLuciferase VectorspRL-null
Eco53kI (10) PaeR7I - XhoI (5) BglII (1) DrdI (3207) PspFI (3009) BseYI (3005) AlwNI (2900) AhdI (2421) BpmI (2352) BsrFI (2336) BglI (2303) NmeAIII (2274) FspI (2198) PvuI (2052) BanII - SacI (12) HindIII (15) NdeI (24) NsiI (30) SphI (32) SpeI (34) KasI (40) NarI (41) SfoI (42) PluTI (44) SalI (47) AccI (48) MluI (51) EcoRI (58) TspMI - XmaI (65) SmaI (67) PstI (77) BfuAI - BspMI (91) AflII (264) T7 promoter NheI (299) BmtI (303) BstBI (315) BstAPI (441) PciI (491) XcmI (958) BsrGI (1007) BsaAI (1041) XbaI (1246) EagI - NotI (1253) HpaI (1396) MfeI (1405) BspDI - ClaI (1491) BsaBI * (1497) BamHI (1498) SspI (1616) pRL-null 3320 bp
Eco53kI  (10)
1 site		 G A G C T C C T C G A G
PaeR7I  (5)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (5)
1 site		 C T C G A G G A G C T C
BglII  (1)
1 site		 A G A T C T T C T A G A
DrdI  (3207)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
PspFI  (3009)
1 site		 C C C A G C G G G T C G
BseYI  (3005)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AlwNI  (2900)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
AhdI  (2421)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BpmI  (2352)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BsrFI  (2336)
1 site		 R C C G G Y Y G G C C R
Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BglI  (2303)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
NmeAIII  (2274)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
FspI  (2198)
1 site		 T G C G C A A C G C G T
PvuI  (2052)
1 site		 C G A T C G G C T A G C
BanII  (12)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
SacI  (12)
1 site		 G A G C T C C T C G A G
HindIII  (15)
1 site		 A A G C T T T T C G A A
NdeI  (24)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
NsiI  (30)
1 site		 A T G C A T T A C G T A
SphI  (32)
1 site		 G C A T G C C G T A C G
SpeI  (34)
1 site		 A C T A G T T G A T C A
KasI  (40)
1 site		 G G C G C C C C G C G G
NarI  (41)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (42)
1 site		 G G C G C C C C G C G G
PluTI  (44)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SalI  (47)
1 site		 G T C G A C C A G C T G
AccI  (48)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
MluI  (51)
1 site		 A C G C G T T G C G C A
EcoRI  (58)
1 site		 G A A T T C C T T A A G
TspMI  (65)
1 site		 C C C G G G G G G C C C
XmaI  (65)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (67)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
PstI  (77)
1 site		 C T G C A G G A C G T C
BfuAI  (91)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (91)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
AflII  (264)
1 site		 C T T A A G G A A T T C
NheI  (299)
1 site		 G C T A G C C G A T C G
BmtI  (303)
1 site		 G C T A G C C G A T C G
BstBI  (315)
1 site		 T T C G A A A A G C T T
BstAPI  (441)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
PciI  (491)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
XcmI  (958)
1 site		 C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C
The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BsrGI  (1007)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
BsaAI  (1041)
1 site		 Y A C G T R R T G C A Y
XbaI  (1246)
1 site		 T C T A G A A G A T C T
EagI  (1253)
1 site		 C G G C C G G C C G G C
NotI  (1253)
1 site		 G C G G C C G C C G C C G G C G
HpaI  (1396)
1 site		 G T T A A C C A A T T G
MfeI  (1405)
1 site		 C A A T T G G T T A A C
BspDI  (1491)
1 site		 A T C G A T T A G C T A
ClaI  (1491)
1 site		 A T C G A T T A G C T A
BsaBI  (1497)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BamHI  (1498)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SspI  (1616)
1 site		 A A T A T T T T A T A A
Rluc
309 .. 1244  =  936 bp
311 amino acids  =  36.0 kDa
Product: luciferase from the anthozoan coelenterate Renilla reniformis (sea pansy)
Rluc
309 .. 1244  =  936 bp
311 amino acids  =  36.0 kDa
Product: luciferase from the anthozoan coelenterate Renilla reniformis (sea pansy)
AmpR
1634 .. 2494  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   1634 .. 1702  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1634 .. 2494  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1703 .. 2494  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1634 .. 2494  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
2665 .. 3253  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2665 .. 3253  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
chimeric intron
104 .. 236  =  133 bp
chimera between introns from human β-globin and immunoglobulin heavy chain genes
chimeric intron
104 .. 236  =  133 bp
chimera between introns from human β-globin and immunoglobulin heavy chain genes
SV40 poly(A) signal
1275 .. 1396  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1275 .. 1396  =  122 bp
SV40 polyadenylation signal
AmpR promoter
1529 .. 1633  =  105 bp
AmpR promoter
1529 .. 1633  =  105 bp
MCS
1 .. 78  =  78 bp
multiple cloning site
MCS
1 .. 78  =  78 bp
multiple cloning site
T7 promoter
281 .. 299  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
281 .. 299  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  1634 .. 2494  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  309 .. 1244  =  936 bp
ORF:  311 amino acids  =  36.0 kDa
ORF:  2098 .. 2364  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
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Individual Sequences & Maps

pBI-CMV5
pGL4.22[luc2CP Puro]
pGL4.81[hRlucCP Neo]
pRL-null
pBIND-ER(alpha)
pGL4.23[luc2 minP]
pGL4.82[hRluc Puro]
pRL-SV40
pBIND-GR
pGL4.24[luc2P minP]
pGL4.83[hRlucP Puro]
pRL-TK
pCBG68-Basic
pGL4.25[luc2CP minP]
pGL4.84[hRlucCP Puro]
pSELECT-zeo-Lucia
pCBG68-Control
pGL4.26[luc2 minP Hygro]
pGLuc Mini-TK 2
pSF-CMV-EMCV-FLuc
pCBG99-Basic
pGL4.27[luc2P minP Hygro]
pGLuc-Basic 2
pSF-CMV-EMCV-RLuc
pCBG99-Control
pGL4.28[luc2CP minP Hygro]
phRG-B
pSF-CMV-FLuc
pCBR-Basic
pGL4.29[luc2P CRE Hygro]
phRG-TK
pSF-CMV-FMDV-FLuc
pCBR-Control
pGL4.30[luc2P NFAT-RE Hygro]
phRL-CMV
pSF-CMV-FMDV-RLuc
pCLuc Mini-TK 2
pGL4.31[luc2P GAL4UAS Hygro]
phRL-null
pSF-CMV-PGK-FLuc
pCLuc-Basic 2
pGL4.32[luc2P NF-kB-RE Hygro]
phRL-SV40
pSF-CMV-PGK-RLuc
pCMV-CLuc 2
pGL4.33[luc2P SRE Hygro]
phRL-TK
pSF-CMV-RLuc
pCMV-Cypridina Luc
pGL4.34[luc2P SRF-RE Hygro]
phRL-TK(Int-)
pSF-CMV-RSV-FLuc AscI
pCMV-Gaussia Luc
pGL4.35[luc2P 9XGAL4UAS Hygro]
pLightSwitch_Prom
pSF-CMV-RSV-RLuc AscI
pCMV-Gaussia-Dura Luc
pGL4.36[luc2P MMTV Hygro]
pLVX-MetLuc Control
pSF-CMV-Ub-FLuc AscI
pCMV-GLuc 2
pGL4.37[luc2P ARE Hygro]
pLVX-MetLuc Reporter
pSF-CMV-Ub-RLuc AscI
pCMV-Green Renilla Luc
pGL4.38[luc2P p53 RE Hygro]
pMCS-Cypridina Luc
pSF-CMVe-FLuc
pCMV-Red Firefly Luc
pGL4.39[luc2P ATF6 RE Hygro]
pMCS-Gaussia Luc
pSF-CMVe-RLuc
pCRE-MetLuc2-Reporter
pGL4.40[luc2P MRE Hygro]
pMCS-Gaussia-Dura Luc
pSF-MinProm-FLuc
pFN26A (BIND) hRluc-neo Flexi Vector
pGL4.41[luc2P HSE Hygro]
pMCS-Green Renilla Luc
pSF-MinProm-RLuc
pGEM-luc
pGL4.42[luc2P HRE Hygro]
pMCS-Red Firefly Luc
pSF-pA-CMVe-FLuc
pGL2-Basic
pGL4.43[luc2P XRE Hygro]
pMetLuc-Mem Control
pSF-pA-CMVe-RLuc
pGL2-Control
pGL4.44[luc2P AP1 RE Hygro]
pMetLuc-Mem Reporter
pSF-pA-MinProm-FLuc
pGL2-Enhancer
pGL4.45[luc2P ISRE Hygro]
pMetLuc2-Control
pSF-pA-MinProm-RLuc
pGL2-Promoter
pGL4.47[luc2P SIE Hygro]
pMetLuc2-Reporter
pSF-pA-PromMCS-FLuc
pGL3-Basic
pGL4.48[luc2P SBE Hygro]
pmirGLO
pSF-pA-PromMCS-RLuc
pGL3-Control
pGL4.49[luc2P TCF-LEF RE Hygro]
pMirTarget
pSF-PromMCS-FLuc
pGL3-Enhancer
pGL4.50[luc2 CMV Hygro]
pNFkB-MetLuc2-Reporter
pSF-PromMCS-RLuc
pGL3-Promoter
pGL4.51[luc2 CMV Neo]
pNL1.1.CMV[Nluc CMV]
pSF-RecA-FLuc
pGL4.10[luc2]
pGL4.52[luc2P STAT5 RE Hygro]
pNL1.1[Nluc]
pSP-luc+NF
pGL4.11[luc2P]
pGL4.70[hRluc]
pNL1.2[NlucP]
pSV40-CLuc
pGL4.12[luc2CP]
pGL4.71[hRlucP]
pNL1.3.CMV[secNluc CMV]
pSV40-GLuc
pGL4.13[luc2 SV40]
pGL4.72[hRlucCP]
pNL1.3[secNluc]
pTK-CLuc
pGL4.14[luc2 Hygro]
pGL4.73[hRluc SV40]
pNL2.1[Nluc Hygro]
pTK-Cypridina Luc
pGL4.15[luc2P Hygro]
pGL4.74[hRluc TK]
pNL2.2[NlucP Hygro]
pTK-Gaussia Luc
pGL4.16[luc2CP Hygro]
pGL4.75[hRluc CMV]
pNL2.3[secNluc Hygro]
pTK-Gaussia-Dura Luc
pGL4.17[luc2 Neo]
pGL4.76[hRluc Hygro]
pNL3.1[Nluc minP]
pTK-GLuc
pGL4.18[luc2P Neo]
pGL4.77[hRlucP Hygro]
pNL3.2.NF-kB-RE[NlucP NF-kB-RE Hygro]
pTK-Green Renilla Luc
pGL4.19[luc2CP Neo]
pGL4.78[hRlucCP Hygro]
pNL3.2[NlucP minP]
pTK-Red Firefly Luc
pGL4.20[luc2 Puro]
pGL4.79[hRluc Neo]
pNL3.3[secNluc minP]
pXPG
pGL4.21[luc2P Puro]
pGL4.80[hRlucP Neo]
pRL-CMV

Plasmid Sets

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I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
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Coronavirus Resources
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Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
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Structural Genomics Vectors

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