pmirGLO

Vector for quantitatively analyzing microRNA (miRNA) activity by inserting miRNA binding sites downstream of a firefly luciferase gene.

Sequence Author: Promega

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HomePlasmidsLuciferase VectorspmirGLO
AccI (7339) SalI (7338) XbaI (7332) PaeR7I - PspXI - XhoI (7326) BmtI (7324) NheI (7320) SacI (7318) Eco53kI (7316) DraI - PmeI (7309) BbvCI (6356) AleI (6143) BsrGI (6135) MreI - SgrAI (5708) BbsI (5703) ApaI (5675) PspOMI (5671) PpuMI (5513) BsmBI - Esp3I - SpeI (5344) BglII (5090) MluI (4971) PciI (3994) AhdI (3824) SbfI (7348) NotI (93) PsiI (216) MfeI (245) Acc65I (473) KpnI (477) SV40 promoter SfiI (776) AvrII (823) PflMI (877) PfoI * (1169) EcoRV (1374) Bsu36I (1475) NruI (1550) PasI (1663) BssHII (2395) RsrII (2514) BstXI (2719) poly(A) signal BspDI - ClaI (2779) BamHI (2792) SnaBI (2886) AseI (3649) pmirGLO 7350 bp
AccI  (7339)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (7338)
1 site		 G T C G A C C A G C T G
XbaI  (7332)
1 site		 T C T A G A A G A T C T
PaeR7I  (7326)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (7326)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (7326)
1 site		 C T C G A G G A G C T C
BmtI  (7324)
1 site		 G C T A G C C G A T C G
NheI  (7320)
1 site		 G C T A G C C G A T C G
SacI  (7318)
1 site		 G A G C T C C T C G A G
Eco53kI  (7316)
1 site		 G A G C T C C T C G A G
DraI  (7309)
1 site		 T T T A A A A A A T T T
PmeI  (7309)
1 site		 G T T T A A A C C A A A T T T G
BbvCI  (6356)
1 site		 C C T C A G C G G A G T C G
AleI  (6143)
1 site		 C A C N N N N G T G G T G N N N N C A C
BsrGI  (6135)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
MreI  (5708)
1 site		 C G C C G G C G G C G G C C G C
SgrAI  (5708)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BbsI  (5703)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
ApaI  (5675)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
PspOMI  (5671)
1 site		 G G G C C C C C C G G G
PpuMI  (5513)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
BsmBI  (5344)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (5344)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
SpeI  (5344)
1 site		 A C T A G T T G A T C A
BglII  (5090)
1 site		 A G A T C T T C T A G A
MluI  (4971)
1 site		 A C G C G T T G C G C A
PciI  (3994)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
AhdI  (3824)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SbfI  (7348)
1 site		 C C T G C A G G G G A C G T C C
NotI  (93)
1 site		 G C G G C C G C C G C C G G C G
PsiI  (216)
1 site		 T T A T A A A A T A T T
MfeI  (245)
1 site		 C A A T T G G T T A A C
Acc65I  (473)
1 site		 G G T A C C C C A T G G
KpnI  (477)
1 site		 G G T A C C C C A T G G
SfiI  (776)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AvrII  (823)
1 site		 C C T A G G G G A T C C
PflMI  (877)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
PfoI  (1169)
1 site		 T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
EcoRV  (1374)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
Bsu36I  (1475)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
NruI  (1550)
1 site		 T C G C G A A G C G C T
PasI  (1663)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
BssHII  (2395)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
RsrII  (2514)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BstXI  (2719)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
BspDI  (2779)
1 site		 A T C G A T T A G C T A
ClaI  (2779)
1 site		 A T C G A T T A G C T A
BamHI  (2792)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SnaBI  (2886)
1 site		 T A C G T A A T G C A T
AseI  (3649)
1 site		 A T T A A T T A A T T A
hRluc
889 .. 1821  =  933 bp
311 amino acids  =  36.0 kDa
Product: Renilla luciferase
human codon-optimized
hRluc
889 .. 1821  =  933 bp
311 amino acids  =  36.0 kDa
Product: Renilla luciferase
human codon-optimized
NeoR/KanR
1873 .. 2664  =  792 bp
263 amino acids  =  28.9 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
1873 .. 2664  =  792 bp
263 amino acids  =  28.9 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
luciferase
5645 .. 7297  =  1653 bp
550 amino acids  =  60.6 kDa
Product: firefly luciferase
synthetic luc2 version of the luciferase gene
luciferase
5645 .. 7297  =  1653 bp
550 amino acids  =  60.6 kDa
Product: firefly luciferase
synthetic luc2 version of the luciferase gene
AmpR
3037 .. 3897  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3037 .. 3105  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3037 .. 3897  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   3106 .. 3897  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3037 .. 3897  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
4055 .. 4643  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4055 .. 4643  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
PGK promoter
5099 .. 5598  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
5099 .. 5598  =  500 bp
mouse phosphoglycerate kinase 1 promoter
SV40 promoter
481 .. 838  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
481 .. 838  =  358 bp
SV40 enhancer and early promoter
SV40 poly(A) signal
115 .. 236  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
115 .. 236  =  122 bp
SV40 polyadenylation signal
AmpR promoter
2932 .. 3036  =  105 bp
AmpR promoter
2932 .. 3036  =  105 bp
poly(A) signal
2728 .. 2776  =  49 bp
synthetic polyadenylation signal
poly(A) signal
2728 .. 2776  =  49 bp
synthetic polyadenylation signal
MCS
7306 .. 7350  =  45 bp
multiple cloning site
MCS
7306 .. 7350  =  45 bp
multiple cloning site
SV40 ori
689 .. 824  =  136 bp
SV40 origin of replication
SV40 ori
689 .. 824  =  136 bp
SV40 origin of replication
ORF:  889 .. 2664  =  1776 bp
ORF:  591 amino acids  =  66.0 kDa
ORF:  3037 .. 3897  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  2042 .. 2428  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  5645 .. 7297  =  1653 bp
ORF:  550 amino acids  =  60.6 kDa
ORF:  5379 .. 5735  =  357 bp
ORF:  118 amino acids  =  12.8 kDa
ORF:  754 .. 1041  =  288 bp
ORF:  95 amino acids  =  10.2 kDa
ORF:  2179 .. 2715  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
ORF:  3501 .. 3767  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2540 .. 2845  =  306 bp
ORF:  101 amino acids  =  11.5 kDa
ORF:  5489 .. 5872  =  384 bp
ORF:  127 amino acids  =  13.9 kDa
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Individual Sequences & Maps

pBI-CMV5
pGL4.22[luc2CP Puro]
pGL4.81[hRlucCP Neo]
pRL-null
pBIND-ER(alpha)
pGL4.23[luc2 minP]
pGL4.82[hRluc Puro]
pRL-SV40
pBIND-GR
pGL4.24[luc2P minP]
pGL4.83[hRlucP Puro]
pRL-TK
pCBG68-Basic
pGL4.25[luc2CP minP]
pGL4.84[hRlucCP Puro]
pSELECT-zeo-Lucia
pCBG68-Control
pGL4.26[luc2 minP Hygro]
pGLuc Mini-TK 2
pSF-CMV-EMCV-FLuc
pCBG99-Basic
pGL4.27[luc2P minP Hygro]
pGLuc-Basic 2
pSF-CMV-EMCV-RLuc
pCBG99-Control
pGL4.28[luc2CP minP Hygro]
phRG-B
pSF-CMV-FLuc
pCBR-Basic
pGL4.29[luc2P CRE Hygro]
phRG-TK
pSF-CMV-FMDV-FLuc
pCBR-Control
pGL4.30[luc2P NFAT-RE Hygro]
phRL-CMV
pSF-CMV-FMDV-RLuc
pCLuc Mini-TK 2
pGL4.31[luc2P GAL4UAS Hygro]
phRL-null
pSF-CMV-PGK-FLuc
pCLuc-Basic 2
pGL4.32[luc2P NF-kB-RE Hygro]
phRL-SV40
pSF-CMV-PGK-RLuc
pCMV-CLuc 2
pGL4.33[luc2P SRE Hygro]
phRL-TK
pSF-CMV-RLuc
pCMV-Cypridina Luc
pGL4.34[luc2P SRF-RE Hygro]
phRL-TK(Int-)
pSF-CMV-RSV-FLuc AscI
pCMV-Gaussia Luc
pGL4.35[luc2P 9XGAL4UAS Hygro]
pLightSwitch_Prom
pSF-CMV-RSV-RLuc AscI
pCMV-Gaussia-Dura Luc
pGL4.36[luc2P MMTV Hygro]
pLVX-MetLuc Control
pSF-CMV-Ub-FLuc AscI
pCMV-GLuc 2
pGL4.37[luc2P ARE Hygro]
pLVX-MetLuc Reporter
pSF-CMV-Ub-RLuc AscI
pCMV-Green Renilla Luc
pGL4.38[luc2P p53 RE Hygro]
pMCS-Cypridina Luc
pSF-CMVe-FLuc
pCMV-Red Firefly Luc
pGL4.39[luc2P ATF6 RE Hygro]
pMCS-Gaussia Luc
pSF-CMVe-RLuc
pCRE-MetLuc2-Reporter
pGL4.40[luc2P MRE Hygro]
pMCS-Gaussia-Dura Luc
pSF-MinProm-FLuc
pFN26A (BIND) hRluc-neo Flexi Vector
pGL4.41[luc2P HSE Hygro]
pMCS-Green Renilla Luc
pSF-MinProm-RLuc
pGEM-luc
pGL4.42[luc2P HRE Hygro]
pMCS-Red Firefly Luc
pSF-pA-CMVe-FLuc
pGL2-Basic
pGL4.43[luc2P XRE Hygro]
pMetLuc-Mem Control
pSF-pA-CMVe-RLuc
pGL2-Control
pGL4.44[luc2P AP1 RE Hygro]
pMetLuc-Mem Reporter
pSF-pA-MinProm-FLuc
pGL2-Enhancer
pGL4.45[luc2P ISRE Hygro]
pMetLuc2-Control
pSF-pA-MinProm-RLuc
pGL2-Promoter
pGL4.47[luc2P SIE Hygro]
pMetLuc2-Reporter
pSF-pA-PromMCS-FLuc
pGL3-Basic
pGL4.48[luc2P SBE Hygro]
pmirGLO
pSF-pA-PromMCS-RLuc
pGL3-Control
pGL4.49[luc2P TCF-LEF RE Hygro]
pMirTarget
pSF-PromMCS-FLuc
pGL3-Enhancer
pGL4.50[luc2 CMV Hygro]
pNFkB-MetLuc2-Reporter
pSF-PromMCS-RLuc
pGL3-Promoter
pGL4.51[luc2 CMV Neo]
pNL1.1.CMV[Nluc CMV]
pSF-RecA-FLuc
pGL4.10[luc2]
pGL4.52[luc2P STAT5 RE Hygro]
pNL1.1[Nluc]
pSP-luc+NF
pGL4.11[luc2P]
pGL4.70[hRluc]
pNL1.2[NlucP]
pSV40-CLuc
pGL4.12[luc2CP]
pGL4.71[hRlucP]
pNL1.3.CMV[secNluc CMV]
pSV40-GLuc
pGL4.13[luc2 SV40]
pGL4.72[hRlucCP]
pNL1.3[secNluc]
pTK-CLuc
pGL4.14[luc2 Hygro]
pGL4.73[hRluc SV40]
pNL2.1[Nluc Hygro]
pTK-Cypridina Luc
pGL4.15[luc2P Hygro]
pGL4.74[hRluc TK]
pNL2.2[NlucP Hygro]
pTK-Gaussia Luc
pGL4.16[luc2CP Hygro]
pGL4.75[hRluc CMV]
pNL2.3[secNluc Hygro]
pTK-Gaussia-Dura Luc
pGL4.17[luc2 Neo]
pGL4.76[hRluc Hygro]
pNL3.1[Nluc minP]
pTK-GLuc
pGL4.18[luc2P Neo]
pGL4.77[hRlucP Hygro]
pNL3.2.NF-kB-RE[NlucP NF-kB-RE Hygro]
pTK-Green Renilla Luc
pGL4.19[luc2CP Neo]
pGL4.78[hRlucCP Hygro]
pNL3.2[NlucP minP]
pTK-Red Firefly Luc
pGL4.20[luc2 Puro]
pGL4.79[hRluc Neo]
pNL3.3[secNluc minP]
pXPG
pGL4.21[luc2P Puro]
pGL4.80[hRlucP Neo]
pRL-CMV

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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