pSP-luc+NF

Enhanced firefly luciferase vector for creating N-terminal fusions and for in vitro transcription.

Sequence Author: Promega

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HomePlasmidsLuciferase VectorspSP-luc+NF
Acc65I (11) NdeI (4020) AatII (3771) ZraI (3769) SspI (3653) XmnI (3448) PvuI (3219) FspI (3071) NmeAIII (2997) BglI (2969) AlwNI (2372) KpnI (15) NheI (17) BmtI (21) BglII (24) AvrII (30) HindIII (36) NcoI (43) BstEII (47) KasI (80) NarI (81) SfoI (82) PluTI (84) BstBI (217) PsiI (373) BsrGI (538) BclI * (628) SphI (711) XcmI (783) BanII (1072) PpuMI (1227) PaqCI (1437) SgrAI (1476) XbaI (1702) EcoRI (1708) EcoRV (1716) BspDI * - ClaI * (1721) PaeR7I - XhoI (1726) T7 promoter HpaI (1777) PciI (1956) pSP-luc+NF 4103 bp
Acc65I  (11)
1 site		 G G T A C C C C A T G G
NdeI  (4020)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
AatII  (3771)
1 site		 G A C G T C C T G C A G
ZraI  (3769)
1 site		 G A C G T C C T G C A G
SspI  (3653)
1 site		 A A T A T T T T A T A A
XmnI  (3448)
1 site		 G A A N N N N T T C C T T N N N N A A G
PvuI  (3219)
1 site		 C G A T C G G C T A G C
FspI  (3071)
1 site		 T G C G C A A C G C G T
NmeAIII  (2997)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (2969)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
AlwNI  (2372)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
KpnI  (15)
1 site		 G G T A C C C C A T G G
NheI  (17)
1 site		 G C T A G C C G A T C G
BmtI  (21)
1 site		 G C T A G C C G A T C G
BglII  (24)
1 site		 A G A T C T T C T A G A
AvrII  (30)
1 site		 C C T A G G G G A T C C
HindIII  (36)
1 site		 A A G C T T T T C G A A
NcoI  (43)
1 site		 C C A T G G G G T A C C
BstEII  (47)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
KasI  (80)
1 site		 G G C G C C C C G C G G
NarI  (81)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (82)
1 site		 G G C G C C C C G C G G
PluTI  (84)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BstBI  (217)
1 site		 T T C G A A A A G C T T
PsiI  (373)
1 site		 T T A T A A A A T A T T
BsrGI  (538)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
BclI  (628)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
SphI  (711)
1 site		 G C A T G C C G T A C G
XcmI  (783)
1 site		 C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C
The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BanII  (1072)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
PpuMI  (1227)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
PaqCI  (1437)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
SgrAI  (1476)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
XbaI  (1702)
1 site		 T C T A G A A G A T C T
EcoRI  (1708)
1 site		 G A A T T C C T T A A G
EcoRV  (1716)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BspDI  (1721)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (1721)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
PaeR7I  (1726)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1726)
1 site		 C T C G A G G A G C T C
HpaI  (1777)
1 site		 G T T A A C C A A T T G
PciI  (1956)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
luciferase
45 .. 1700  =  1656 bp
551 amino acids  =  60.7 kDa
Product: firefly luciferase
enhanced luc+NF version of the luciferase gene
luciferase
45 .. 1700  =  1656 bp
551 amino acids  =  60.7 kDa
Product: firefly luciferase
enhanced luc+NF version of the luciferase gene
AmpR
2776 .. 3636  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2776 .. 3567  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2776 .. 3636  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3568 .. 3636  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2776 .. 3636  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
2017 .. 2605  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2017 .. 2605  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
AmpR promoter
3637 .. 3741  =  105 bp
AmpR promoter
3637 .. 3741  =  105 bp
downstream MCS
1702 .. 1731  =  30 bp
downstream multiple cloning site
downstream MCS
1702 .. 1731  =  30 bp
downstream multiple cloning site
T7 promoter
1741 .. 1759  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1741 .. 1759  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SP6 promoter
4087 .. 2  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
4087 .. 2  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
upstream MCS
11 .. 53  =  43 bp
upstream multiple cloning site
upstream MCS
11 .. 53  =  43 bp
upstream multiple cloning site
ORF:  2906 .. 3172  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  45 .. 1700  =  1656 bp
ORF:  551 amino acids  =  60.7 kDa
ORF:  333 .. 560  =  228 bp
ORF:  75 amino acids  =  9.1 kDa
ORF:  1299 .. 1721  =  423 bp
ORF:  140 amino acids  =  15.6 kDa
ORF:  2776 .. 3636  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Individual Sequences & Maps

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phRG-B
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phRG-TK
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pGL4.31[luc2P GAL4UAS Hygro]
phRL-null
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pGL4.32[luc2P NF-kB-RE Hygro]
phRL-SV40
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pCMV-CLuc 2
pGL4.33[luc2P SRE Hygro]
phRL-TK
pSF-CMV-RLuc
pCMV-Cypridina Luc
pGL4.34[luc2P SRF-RE Hygro]
phRL-TK(Int-)
pSF-CMV-RSV-FLuc AscI
pCMV-Gaussia Luc
pGL4.35[luc2P 9XGAL4UAS Hygro]
pLightSwitch_Prom
pSF-CMV-RSV-RLuc AscI
pCMV-Gaussia-Dura Luc
pGL4.36[luc2P MMTV Hygro]
pLVX-MetLuc Control
pSF-CMV-Ub-FLuc AscI
pCMV-GLuc 2
pGL4.37[luc2P ARE Hygro]
pLVX-MetLuc Reporter
pSF-CMV-Ub-RLuc AscI
pCMV-Green Renilla Luc
pGL4.38[luc2P p53 RE Hygro]
pMCS-Cypridina Luc
pSF-CMVe-FLuc
pCMV-Red Firefly Luc
pGL4.39[luc2P ATF6 RE Hygro]
pMCS-Gaussia Luc
pSF-CMVe-RLuc
pCRE-MetLuc2-Reporter
pGL4.40[luc2P MRE Hygro]
pMCS-Gaussia-Dura Luc
pSF-MinProm-FLuc
pFN26A (BIND) hRluc-neo Flexi Vector
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pSF-MinProm-RLuc
pGEM-luc
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pMCS-Red Firefly Luc
pSF-pA-CMVe-FLuc
pGL2-Basic
pGL4.43[luc2P XRE Hygro]
pMetLuc-Mem Control
pSF-pA-CMVe-RLuc
pGL2-Control
pGL4.44[luc2P AP1 RE Hygro]
pMetLuc-Mem Reporter
pSF-pA-MinProm-FLuc
pGL2-Enhancer
pGL4.45[luc2P ISRE Hygro]
pMetLuc2-Control
pSF-pA-MinProm-RLuc
pGL2-Promoter
pGL4.47[luc2P SIE Hygro]
pMetLuc2-Reporter
pSF-pA-PromMCS-FLuc
pGL3-Basic
pGL4.48[luc2P SBE Hygro]
pmirGLO
pSF-pA-PromMCS-RLuc
pGL3-Control
pGL4.49[luc2P TCF-LEF RE Hygro]
pMirTarget
pSF-PromMCS-FLuc
pGL3-Enhancer
pGL4.50[luc2 CMV Hygro]
pNFkB-MetLuc2-Reporter
pSF-PromMCS-RLuc
pGL3-Promoter
pGL4.51[luc2 CMV Neo]
pNL1.1.CMV[Nluc CMV]
pSF-RecA-FLuc
pGL4.10[luc2]
pGL4.52[luc2P STAT5 RE Hygro]
pNL1.1[Nluc]
pSP-luc+NF
pGL4.11[luc2P]
pGL4.70[hRluc]
pNL1.2[NlucP]
pSV40-CLuc
pGL4.12[luc2CP]
pGL4.71[hRlucP]
pNL1.3.CMV[secNluc CMV]
pSV40-GLuc
pGL4.13[luc2 SV40]
pGL4.72[hRlucCP]
pNL1.3[secNluc]
pTK-CLuc
pGL4.14[luc2 Hygro]
pGL4.73[hRluc SV40]
pNL2.1[Nluc Hygro]
pTK-Cypridina Luc
pGL4.15[luc2P Hygro]
pGL4.74[hRluc TK]
pNL2.2[NlucP Hygro]
pTK-Gaussia Luc
pGL4.16[luc2CP Hygro]
pGL4.75[hRluc CMV]
pNL2.3[secNluc Hygro]
pTK-Gaussia-Dura Luc
pGL4.17[luc2 Neo]
pGL4.76[hRluc Hygro]
pNL3.1[Nluc minP]
pTK-GLuc
pGL4.18[luc2P Neo]
pGL4.77[hRlucP Hygro]
pNL3.2.NF-kB-RE[NlucP NF-kB-RE Hygro]
pTK-Green Renilla Luc
pGL4.19[luc2CP Neo]
pGL4.78[hRlucCP Hygro]
pNL3.2[NlucP minP]
pTK-Red Firefly Luc
pGL4.20[luc2 Puro]
pGL4.79[hRluc Neo]
pNL3.3[secNluc minP]
pXPG
pGL4.21[luc2P Puro]
pGL4.80[hRlucP Neo]
pRL-CMV

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