pGL2-Promoter

SV40 promoter-containing vector for measuring the activity of enhancer sequences with a luciferase assay.

Sequence Author: Promega

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HomePlasmidsLuciferase VectorspGL2-Promoter
GLprimer1 (5757 .. 5779) DraIII (5232) XmnI (4679) ScaI (4560) EaeI (4468) BsaI (4141) AhdI (4080) AlwNI (3603) PciI (3187) AfeI (3063) RVprimer4 (2988 .. 3007) PshAI (3002) AccI (2938) SalI (2937) BamHI (2931) TspMI - XmaI (1) SmaI (3) Acc65I (8) KpnI (12) Eco53kI (16) SacI (18) MluI (22) NheI (28) BmtI (32) PaeR7I - XhoI (33) BglII (37) BtgI - NcoI (130) SfiI (176) StuI (222) AvrII (223) HindIII (239) GLprimer2 (269 .. 291) KasI (300) NarI (301) SfoI (302) PluTI (304) XbaI (315) PfoI * (375) BsiWI (422) BsrGI (758) EcoRI (855) BstEII (875) Bsu36I (881) SphI (931) XcmI (1003) EcoO109I - PpuMI (1447) PacI (1592) EcoRV (1606) BspDI - ClaI (1633) BfuAI - BspMI (1666) SgrAI (1696) EcoNI (1885) PflMI (2236) BsgI (2468) pGL2-Promoter 5790 bp
DraIII  (5232)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
XmnI  (4679)
1 site		 G A A N N N N T T C C T T N N N N A A G
ScaI  (4560)
1 site		 A G T A C T T C A T G A
EaeI  (4468)
1 site		 Y G G C C R R C C G G Y
BsaI  (4141)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (4080)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (3603)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
PciI  (3187)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
AfeI  (3063)
1 site		 A G C G C T T C G C G A
PshAI  (3002)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AccI  (2938)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (2937)
1 site		 G T C G A C C A G C T G
BamHI  (2931)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
TspMI  (1)
1 site		 C C C G G G G G G C C C
XmaI  (1)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (3)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
Acc65I  (8)
1 site		 G G T A C C C C A T G G
KpnI  (12)
1 site		 G G T A C C C C A T G G
Eco53kI  (16)
1 site		 G A G C T C C T C G A G
SacI  (18)
1 site		 G A G C T C C T C G A G
MluI  (22)
1 site		 A C G C G T T G C G C A
NheI  (28)
1 site		 G C T A G C C G A T C G
BmtI  (32)
1 site		 G C T A G C C G A T C G
PaeR7I  (33)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (33)
1 site		 C T C G A G G A G C T C
BglII  (37)
1 site		 A G A T C T T C T A G A
BtgI  (130)
1 site		 C C R Y G G G G Y R C C
Sticky ends from different BtgI sites may not be compatible.
NcoI  (130)
1 site		 C C A T G G G G T A C C
SfiI  (176)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
StuI  (222)
1 site		 A G G C C T T C C G G A
AvrII  (223)
1 site		 C C T A G G G G A T C C
HindIII  (239)
1 site		 A A G C T T T T C G A A
KasI  (300)
1 site		 G G C G C C C C G C G G
NarI  (301)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (302)
1 site		 G G C G C C C C G C G G
PluTI  (304)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
XbaI  (315)
1 site		 T C T A G A A G A T C T
PfoI  (375)
1 site		 T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BsiWI  (422)
1 site		 C G T A C G G C A T G C
BsiWI is typically used at 55°C, but is 50% active at 37°C.
BsrGI  (758)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
EcoRI  (855)
1 site		 G A A T T C C T T A A G
BstEII  (875)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
Bsu36I  (881)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
SphI  (931)
1 site		 G C A T G C C G T A C G
XcmI  (1003)
1 site		 C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C
The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
EcoO109I  (1447)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
PpuMI  (1447)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
PacI  (1592)
1 site		 T T A A T T A A A A T T A A T T
EcoRV  (1606)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BspDI  (1633)
1 site		 A T C G A T T A G C T A
ClaI  (1633)
1 site		 A T C G A T T A G C T A
BfuAI  (1666)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (1666)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
SgrAI  (1696)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
EcoNI  (1885)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PflMI  (2236)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
BsgI  (2468)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
GLprimer1
23-mer  /  35% GC
1 binding site
5757 .. 5779  =  23 annealed bases
Tm  =  51°C
RVprimer4
20-mer  /  65% GC
1 binding site
2988 .. 3007  =  20 annealed bases
Tm  =  62°C
GLprimer2
23-mer  /  39% GC
1 binding site
269 .. 291  =  23 annealed bases
Tm  =  57°C
luciferase
268 .. 1920  =  1653 bp
550 amino acids  =  60.7 kDa
Product: firefly luciferase
luciferase
268 .. 1920  =  1653 bp
550 amino acids  =  60.7 kDa
Product: firefly luciferase
AmpR
4007 .. 4867  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4007 .. 4798  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4007 .. 4867  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   4799 .. 4867  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4007 .. 4867  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
3248 .. 3836  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
ori
3248 .. 3836  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
f1 ori
4999 .. 5454  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
4999 .. 5454  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SV40 promoter
42 .. 238  =  197 bp
SV40 early promoter
SV40 promoter
42 .. 238  =  197 bp
SV40 early promoter
SV40 poly(A) signal
2801 .. 2935  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2801 .. 2935  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
5644 .. 5765  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
5644 .. 5765  =  122 bp
SV40 polyadenylation signal
AmpR promoter
4868 .. 4972  =  105 bp
AmpR promoter
4868 .. 4972  =  105 bp
small t intron
2161 .. 2226  =  66 bp
simian virus 40 (SV40) small t antigen intron
small t intron
2161 .. 2226  =  66 bp
simian virus 40 (SV40) small t antigen intron
SV40 ori
89 .. 224  =  136 bp
SV40 origin of replication
SV40 ori
89 .. 224  =  136 bp
SV40 origin of replication
MCS
1 .. 42  =  42 bp
multiple cloning site
MCS
1 .. 42  =  42 bp
multiple cloning site
ORF:  268 .. 1920  =  1653 bp
ORF:  550 amino acids  =  60.7 kDa
ORF:  2305 .. 2778  =  474 bp
ORF:  157 amino acids  =  18.2 kDa
ORF:  4137 .. 4403  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  4007 .. 4867  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Individual Sequences & Maps

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pGL4.22[luc2CP Puro]
pGL4.81[hRlucCP Neo]
pRL-null
pBIND-ER(alpha)
pGL4.23[luc2 minP]
pGL4.82[hRluc Puro]
pRL-SV40
pBIND-GR
pGL4.24[luc2P minP]
pGL4.83[hRlucP Puro]
pRL-TK
pCBG68-Basic
pGL4.25[luc2CP minP]
pGL4.84[hRlucCP Puro]
pSELECT-zeo-Lucia
pCBG68-Control
pGL4.26[luc2 minP Hygro]
pGLuc Mini-TK 2
pSF-CMV-EMCV-FLuc
pCBG99-Basic
pGL4.27[luc2P minP Hygro]
pGLuc-Basic 2
pSF-CMV-EMCV-RLuc
pCBG99-Control
pGL4.28[luc2CP minP Hygro]
phRG-B
pSF-CMV-FLuc
pCBR-Basic
pGL4.29[luc2P CRE Hygro]
phRG-TK
pSF-CMV-FMDV-FLuc
pCBR-Control
pGL4.30[luc2P NFAT-RE Hygro]
phRL-CMV
pSF-CMV-FMDV-RLuc
pCLuc Mini-TK 2
pGL4.31[luc2P GAL4UAS Hygro]
phRL-null
pSF-CMV-PGK-FLuc
pCLuc-Basic 2
pGL4.32[luc2P NF-kB-RE Hygro]
phRL-SV40
pSF-CMV-PGK-RLuc
pCMV-CLuc 2
pGL4.33[luc2P SRE Hygro]
phRL-TK
pSF-CMV-RLuc
pCMV-Cypridina Luc
pGL4.34[luc2P SRF-RE Hygro]
phRL-TK(Int-)
pSF-CMV-RSV-FLuc AscI
pCMV-Gaussia Luc
pGL4.35[luc2P 9XGAL4UAS Hygro]
pLightSwitch_Prom
pSF-CMV-RSV-RLuc AscI
pCMV-Gaussia-Dura Luc
pGL4.36[luc2P MMTV Hygro]
pLVX-MetLuc Control
pSF-CMV-Ub-FLuc AscI
pCMV-GLuc 2
pGL4.37[luc2P ARE Hygro]
pLVX-MetLuc Reporter
pSF-CMV-Ub-RLuc AscI
pCMV-Green Renilla Luc
pGL4.38[luc2P p53 RE Hygro]
pMCS-Cypridina Luc
pSF-CMVe-FLuc
pCMV-Red Firefly Luc
pGL4.39[luc2P ATF6 RE Hygro]
pMCS-Gaussia Luc
pSF-CMVe-RLuc
pCRE-MetLuc2-Reporter
pGL4.40[luc2P MRE Hygro]
pMCS-Gaussia-Dura Luc
pSF-MinProm-FLuc
pFN26A (BIND) hRluc-neo Flexi Vector
pGL4.41[luc2P HSE Hygro]
pMCS-Green Renilla Luc
pSF-MinProm-RLuc
pGEM-luc
pGL4.42[luc2P HRE Hygro]
pMCS-Red Firefly Luc
pSF-pA-CMVe-FLuc
pGL2-Basic
pGL4.43[luc2P XRE Hygro]
pMetLuc-Mem Control
pSF-pA-CMVe-RLuc
pGL2-Control
pGL4.44[luc2P AP1 RE Hygro]
pMetLuc-Mem Reporter
pSF-pA-MinProm-FLuc
pGL2-Enhancer
pGL4.45[luc2P ISRE Hygro]
pMetLuc2-Control
pSF-pA-MinProm-RLuc
pGL2-Promoter
pGL4.47[luc2P SIE Hygro]
pMetLuc2-Reporter
pSF-pA-PromMCS-FLuc
pGL3-Basic
pGL4.48[luc2P SBE Hygro]
pmirGLO
pSF-pA-PromMCS-RLuc
pGL3-Control
pGL4.49[luc2P TCF-LEF RE Hygro]
pMirTarget
pSF-PromMCS-FLuc
pGL3-Enhancer
pGL4.50[luc2 CMV Hygro]
pNFkB-MetLuc2-Reporter
pSF-PromMCS-RLuc
pGL3-Promoter
pGL4.51[luc2 CMV Neo]
pNL1.1.CMV[Nluc CMV]
pSF-RecA-FLuc
pGL4.10[luc2]
pGL4.52[luc2P STAT5 RE Hygro]
pNL1.1[Nluc]
pSP-luc+NF
pGL4.11[luc2P]
pGL4.70[hRluc]
pNL1.2[NlucP]
pSV40-CLuc
pGL4.12[luc2CP]
pGL4.71[hRlucP]
pNL1.3.CMV[secNluc CMV]
pSV40-GLuc
pGL4.13[luc2 SV40]
pGL4.72[hRlucCP]
pNL1.3[secNluc]
pTK-CLuc
pGL4.14[luc2 Hygro]
pGL4.73[hRluc SV40]
pNL2.1[Nluc Hygro]
pTK-Cypridina Luc
pGL4.15[luc2P Hygro]
pGL4.74[hRluc TK]
pNL2.2[NlucP Hygro]
pTK-Gaussia Luc
pGL4.16[luc2CP Hygro]
pGL4.75[hRluc CMV]
pNL2.3[secNluc Hygro]
pTK-Gaussia-Dura Luc
pGL4.17[luc2 Neo]
pGL4.76[hRluc Hygro]
pNL3.1[Nluc minP]
pTK-GLuc
pGL4.18[luc2P Neo]
pGL4.77[hRlucP Hygro]
pNL3.2.NF-kB-RE[NlucP NF-kB-RE Hygro]
pTK-Green Renilla Luc
pGL4.19[luc2CP Neo]
pGL4.78[hRlucCP Hygro]
pNL3.2[NlucP minP]
pTK-Red Firefly Luc
pGL4.20[luc2 Puro]
pGL4.79[hRluc Neo]
pNL3.3[secNluc minP]
pXPG
pGL4.21[luc2P Puro]
pGL4.80[hRlucP Neo]
pRL-CMV

Plasmid Sets

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Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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