pSF-CMV-RLuc

Mammalian vector for strong constitutive expression of Renilla luciferase.

Sequence Author: Oxford Genetics

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HomePlasmidsLuciferase VectorspSF-CMV-RLuc
BtgI (5157) PmeI (5103) AhdI (4866) BsaI (4800) BpmI (4797) PmeI (3982) PpuMI - SanDI (3916) AscI (3825) FseI (3679) NaeI (3677) NgoMIV (3675) SwaI (3573) BspHI (3404) AlwNI (3100) BssSI (2857) DrdI (2792) SwaI (2680) AsiSI - PvuI (5) BglII (232) CMV enhancer SnaBI (566) BglII (816) EagI - NotI (858) HindIII (869) Eco53kI (879) BanII - SacI (881) EcoRI (885) Acc65I (897) KpnI (901) BstAPI (1095) BfuAI - BspMI (1241) Bpu10I (1555) BseRI - BsgI (1844) stop codons BspDI - ClaI (1881) BamHI (1890) StuI (1900) NheI (1906) BmtI (1910) stop codons HpaI (2076) MfeI (2085) PstI - SbfI (2422) 3' β-globin insulator PacI (2554) pSF-CMV-RLuc 5222 bp
BtgI  (5157)
1 site		 C C R Y G G G G Y R C C
Sticky ends from different BtgI sites may not be compatible.
PmeI  (5103)
2 sites		 G T T T A A A C C A A A T T T G
AhdI  (4866)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BsaI  (4800)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BpmI  (4797)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
PmeI  (3982)
2 sites		 G T T T A A A C C A A A T T T G
PpuMI  (3916)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
SanDI  (3916)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different SanDI sites may not be compatible.
AscI  (3825)
1 site		 G G C G C G C C C C G C G C G G
FseI  (3679)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
NaeI  (3677)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (3675)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
SwaI  (3573)
2 sites		 A T T T A A A T T A A A T T T A
SwaI is typically used at 25°C, but is 50% active at 37°C.
BspHI  (3404)
1 site		 T C A T G A A G T A C T
AlwNI  (3100)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
BssSI  (2857)
1 site		 C A C G A G G T G C T C
DrdI  (2792)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
SwaI  (2680)
2 sites		 A T T T A A A T T A A A T T T A
SwaI is typically used at 25°C, but is 50% active at 37°C.
AsiSI  (5)
1 site		 G C G A T C G C C G C T A G C G
PvuI  (5)
1 site		 C G A T C G G C T A G C
BglII  (232)
2 sites		 A G A T C T T C T A G A
SnaBI  (566)
1 site		 T A C G T A A T G C A T
BglII  (816)
2 sites		 A G A T C T T C T A G A
EagI  (858)
1 site		 C G G C C G G C C G G C
NotI  (858)
1 site		 G C G G C C G C C G C C G G C G
HindIII  (869)
1 site		 A A G C T T T T C G A A
Eco53kI  (879)
1 site		 G A G C T C C T C G A G
BanII  (881)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
SacI  (881)
1 site		 G A G C T C C T C G A G
EcoRI  (885)
1 site		 G A A T T C C T T A A G
Acc65I  (897)
1 site		 G G T A C C C C A T G G
KpnI  (901)
1 site		 G G T A C C C C A T G G
BstAPI  (1095)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
BfuAI  (1241)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (1241)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
Bpu10I  (1555)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BseRI  (1844)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BsgI  (1844)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BspDI  (1881)
1 site		 A T C G A T T A G C T A
ClaI  (1881)
1 site		 A T C G A T T A G C T A
BamHI  (1890)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
StuI  (1900)
1 site		 A G G C C T T C C G G A
NheI  (1906)
1 site		 G C T A G C C G A T C G
BmtI  (1910)
1 site		 G C T A G C C G A T C G
HpaI  (2076)
1 site		 G T T A A C C A A T T G
MfeI  (2085)
1 site		 C A A T T G G T T A A C
PstI  (2422)
1 site		 C T G C A G G A C G T C
SbfI  (2422)
1 site		 C C T G C A G G G G A C G T C C
PacI  (2554)
1 site		 T T A A T T A A A A T T A A T T
RLuc
907 .. 1845  =  939 bp
312 amino acids  =  36.1 kDa
Product: Renilla luciferase
codon-optimized synthetic gene
RLuc
907 .. 1845  =  939 bp
312 amino acids  =  36.1 kDa
Product: Renilla luciferase
codon-optimized synthetic gene
AmpR
4079 .. 4939  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   4079 .. 4147  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4079 .. 4939  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4148 .. 4939  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4079 .. 4939  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
2745 .. 3333  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2745 .. 3333  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CMV enhancer
287 .. 590  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
287 .. 590  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
591 .. 794  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
591 .. 794  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
rrnG terminator
2267 .. 2403  =  137 bp
transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)
rrnG terminator
2267 .. 2403  =  137 bp
transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)
rrnG terminator
4952 .. 5088  =  137 bp
transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)
rrnG terminator
4952 .. 5088  =  137 bp
transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)
SV40 poly(A) signal
1955 .. 2076  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1955 .. 2076  =  122 bp
SV40 polyadenylation signal
5' β-globin insulator
18 .. 89  =  72 bp
insulator upstream of the human β-globin locus (Farrell et al., 2002)
5' β-globin insulator
18 .. 89  =  72 bp
insulator upstream of the human β-globin locus (Farrell et al., 2002)
3' β-globin insulator
2448 .. 2519  =  72 bp
insulator downstream of the human β-globin locus (Farrell et al., 2002)
3' β-globin insulator
2448 .. 2519  =  72 bp
insulator downstream of the human β-globin locus (Farrell et al., 2002)
T7 terminator
2191 .. 2237  =  47 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
2191 .. 2237  =  47 bp
transcription terminator for bacteriophage T7 RNA polymerase
stop codons
1865 .. 1875  =  11 bp
stop codons in all three reading frames
stop codons
1865 .. 1875  =  11 bp
stop codons in all three reading frames
stop codons
1913 .. 1923  =  11 bp
stop codons in all three reading frames
stop codons
1913 .. 1923  =  11 bp
stop codons in all three reading frames
RBS
893 .. 898  =  6 bp
Shine-Dalgarno ribosome binding site
RBS
893 .. 898  =  6 bp
Shine-Dalgarno ribosome binding site
RBS
4066 .. 4071  =  6 bp
Shine-Dalgarno ribosome binding site
RBS
4066 .. 4071  =  6 bp
Shine-Dalgarno ribosome binding site
Kozak sequence
903 .. 909  =  7 bp
Kozak sequence
903 .. 909  =  7 bp
ORF:  907 .. 1845  =  939 bp
ORF:  312 amino acids  =  36.1 kDa
ORF:  4079 .. 4939  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  4970 .. 59  =  312 bp
ORF:  103 amino acids  =  11.6 kDa
ORF:  2292 .. 2723  =  432 bp
ORF:  143 amino acids  =  16.3 kDa
ORF:  889 .. 1761  =  873 bp
ORF:  290 amino acids  =  34.7 kDa
ORF:  4543 .. 4809  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
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Individual Sequences & Maps

pBI-CMV5
pGL4.22[luc2CP Puro]
pGL4.81[hRlucCP Neo]
pRL-null
pBIND-ER(alpha)
pGL4.23[luc2 minP]
pGL4.82[hRluc Puro]
pRL-SV40
pBIND-GR
pGL4.24[luc2P minP]
pGL4.83[hRlucP Puro]
pRL-TK
pCBG68-Basic
pGL4.25[luc2CP minP]
pGL4.84[hRlucCP Puro]
pSELECT-zeo-Lucia
pCBG68-Control
pGL4.26[luc2 minP Hygro]
pGLuc Mini-TK 2
pSF-CMV-EMCV-FLuc
pCBG99-Basic
pGL4.27[luc2P minP Hygro]
pGLuc-Basic 2
pSF-CMV-EMCV-RLuc
pCBG99-Control
pGL4.28[luc2CP minP Hygro]
phRG-B
pSF-CMV-FLuc
pCBR-Basic
pGL4.29[luc2P CRE Hygro]
phRG-TK
pSF-CMV-FMDV-FLuc
pCBR-Control
pGL4.30[luc2P NFAT-RE Hygro]
phRL-CMV
pSF-CMV-FMDV-RLuc
pCLuc Mini-TK 2
pGL4.31[luc2P GAL4UAS Hygro]
phRL-null
pSF-CMV-PGK-FLuc
pCLuc-Basic 2
pGL4.32[luc2P NF-kB-RE Hygro]
phRL-SV40
pSF-CMV-PGK-RLuc
pCMV-CLuc 2
pGL4.33[luc2P SRE Hygro]
phRL-TK
pSF-CMV-RLuc
pCMV-Cypridina Luc
pGL4.34[luc2P SRF-RE Hygro]
phRL-TK(Int-)
pSF-CMV-RSV-FLuc AscI
pCMV-Gaussia Luc
pGL4.35[luc2P 9XGAL4UAS Hygro]
pLightSwitch_Prom
pSF-CMV-RSV-RLuc AscI
pCMV-Gaussia-Dura Luc
pGL4.36[luc2P MMTV Hygro]
pLVX-MetLuc Control
pSF-CMV-Ub-FLuc AscI
pCMV-GLuc 2
pGL4.37[luc2P ARE Hygro]
pLVX-MetLuc Reporter
pSF-CMV-Ub-RLuc AscI
pCMV-Green Renilla Luc
pGL4.38[luc2P p53 RE Hygro]
pMCS-Cypridina Luc
pSF-CMVe-FLuc
pCMV-Red Firefly Luc
pGL4.39[luc2P ATF6 RE Hygro]
pMCS-Gaussia Luc
pSF-CMVe-RLuc
pCRE-MetLuc2-Reporter
pGL4.40[luc2P MRE Hygro]
pMCS-Gaussia-Dura Luc
pSF-MinProm-FLuc
pFN26A (BIND) hRluc-neo Flexi Vector
pGL4.41[luc2P HSE Hygro]
pMCS-Green Renilla Luc
pSF-MinProm-RLuc
pGEM-luc
pGL4.42[luc2P HRE Hygro]
pMCS-Red Firefly Luc
pSF-pA-CMVe-FLuc
pGL2-Basic
pGL4.43[luc2P XRE Hygro]
pMetLuc-Mem Control
pSF-pA-CMVe-RLuc
pGL2-Control
pGL4.44[luc2P AP1 RE Hygro]
pMetLuc-Mem Reporter
pSF-pA-MinProm-FLuc
pGL2-Enhancer
pGL4.45[luc2P ISRE Hygro]
pMetLuc2-Control
pSF-pA-MinProm-RLuc
pGL2-Promoter
pGL4.47[luc2P SIE Hygro]
pMetLuc2-Reporter
pSF-pA-PromMCS-FLuc
pGL3-Basic
pGL4.48[luc2P SBE Hygro]
pmirGLO
pSF-pA-PromMCS-RLuc
pGL3-Control
pGL4.49[luc2P TCF-LEF RE Hygro]
pMirTarget
pSF-PromMCS-FLuc
pGL3-Enhancer
pGL4.50[luc2 CMV Hygro]
pNFkB-MetLuc2-Reporter
pSF-PromMCS-RLuc
pGL3-Promoter
pGL4.51[luc2 CMV Neo]
pNL1.1.CMV[Nluc CMV]
pSF-RecA-FLuc
pGL4.10[luc2]
pGL4.52[luc2P STAT5 RE Hygro]
pNL1.1[Nluc]
pSP-luc+NF
pGL4.11[luc2P]
pGL4.70[hRluc]
pNL1.2[NlucP]
pSV40-CLuc
pGL4.12[luc2CP]
pGL4.71[hRlucP]
pNL1.3.CMV[secNluc CMV]
pSV40-GLuc
pGL4.13[luc2 SV40]
pGL4.72[hRlucCP]
pNL1.3[secNluc]
pTK-CLuc
pGL4.14[luc2 Hygro]
pGL4.73[hRluc SV40]
pNL2.1[Nluc Hygro]
pTK-Cypridina Luc
pGL4.15[luc2P Hygro]
pGL4.74[hRluc TK]
pNL2.2[NlucP Hygro]
pTK-Gaussia Luc
pGL4.16[luc2CP Hygro]
pGL4.75[hRluc CMV]
pNL2.3[secNluc Hygro]
pTK-Gaussia-Dura Luc
pGL4.17[luc2 Neo]
pGL4.76[hRluc Hygro]
pNL3.1[Nluc minP]
pTK-GLuc
pGL4.18[luc2P Neo]
pGL4.77[hRlucP Hygro]
pNL3.2.NF-kB-RE[NlucP NF-kB-RE Hygro]
pTK-Green Renilla Luc
pGL4.19[luc2CP Neo]
pGL4.78[hRlucCP Hygro]
pNL3.2[NlucP minP]
pTK-Red Firefly Luc
pGL4.20[luc2 Puro]
pGL4.79[hRluc Neo]
pNL3.3[secNluc minP]
pXPG
pGL4.21[luc2P Puro]
pGL4.80[hRlucP Neo]
pRL-CMV

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