pTK-GLuc

Mammalian vector with a constitutive HSV thymidine kinase promoter for expressing secreted Gaussia luciferase.

Sequence Author: New England Biolabs

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HomePlasmidsLuciferase VectorspTK-GLuc
SgrDI (5895) ZraI (5894) SspI (5778) ScaI (5454) TatI (5452) PciI (4081) PfoI (3505) RsrII (3246) AatII (5896) BglII (12) EcoO109I - PpuMI (330) EcoRI (660) MluI (724) HindIII (771) Acc65I (777) KpnI (781) Eco53kI (785) SacI (787) BamHI (789) NruI (907) NotI (1361) AgeI (1368) PaeR7I - XhoI (1374) XbaI (1386) HpaI (1528) MfeI (1537) BspDI - ClaI (1623) DraIII (1996) CsiI - SexAI * (2287) BseRI (2516) StuI (2519) BclI * (2571) PflFI - Tth111I (2848) pTK-GLuc 5897 bp
SgrDI  (5895)
1 site		 C G T C G A C G G C A G C T G C
ZraI  (5894)
1 site		 G A C G T C C T G C A G
SspI  (5778)
1 site		 A A T A T T T T A T A A
ScaI  (5454)
1 site		 A G T A C T T C A T G A
TatI  (5452)
1 site		 W G T A C W W C A T G W
PciI  (4081)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
PfoI  (3505)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
RsrII  (3246)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AatII  (5896)
1 site		 G A C G T C C T G C A G
BglII  (12)
1 site		 A G A T C T T C T A G A
EcoO109I  (330)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
PpuMI  (330)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
EcoRI  (660)
1 site		 G A A T T C C T T A A G
MluI  (724)
1 site		 A C G C G T T G C G C A
HindIII  (771)
1 site		 A A G C T T T T C G A A
Acc65I  (777)
1 site		 G G T A C C C C A T G G
KpnI  (781)
1 site		 G G T A C C C C A T G G
Eco53kI  (785)
1 site		 G A G C T C C T C G A G
SacI  (787)
1 site		 G A G C T C C T C G A G
BamHI  (789)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
NruI  (907)
1 site		 T C G C G A A G C G C T
NotI  (1361)
1 site		 G C G G C C G C C G C C G G C G
AgeI  (1368)
1 site		 A C C G G T T G G C C A
PaeR7I  (1374)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1374)
1 site		 C T C G A G G A G C T C
XbaI  (1386)
1 site		 T C T A G A A G A T C T
HpaI  (1528)
1 site		 G T T A A C C A A T T G
MfeI  (1537)
1 site		 C A A T T G G T T A A C
BspDI  (1623)
1 site		 A T C G A T T A G C T A
ClaI  (1623)
1 site		 A T C G A T T A G C T A
DraIII  (1996)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
CsiI  (2287)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (2287)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BseRI  (2516)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
StuI  (2519)
1 site		 A G G C C T T C C G G A
BclI  (2571)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
PflFI  (2848)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2848)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
AmpR
4901 .. 5761  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4901 .. 5692  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4901 .. 5761  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   5693 .. 5761  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4901 .. 5761  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR
2602 .. 3396  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
2602 .. 3396  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
HSV TK promoter
19 .. 770  =  752 bp
herpes simplex virus thymidine kinase promoter
HSV TK promoter
19 .. 770  =  752 bp
herpes simplex virus thymidine kinase promoter
ori
4142 .. 4730  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4142 .. 4730  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
hGLuc
802 .. 1359  =  558 bp
185 amino acids  =  19.9 kDa
Product: secreted Gaussia luciferase
human codon-optimized
hGLuc
802 .. 1359  =  558 bp
185 amino acids  =  19.9 kDa
Product: secreted Gaussia luciferase
human codon-optimized
f1 ori
1763 .. 2191  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
1763 .. 2191  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SV40 promoter
2205 .. 2535  =  331 bp
SV40 enhancer and early promoter
SV40 promoter
2205 .. 2535  =  331 bp
SV40 enhancer and early promoter
SV40 poly(A) signal
1407 .. 1528  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1407 .. 1528  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3570 .. 3691  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3570 .. 3691  =  122 bp
SV40 polyadenylation signal
AmpR promoter
5762 .. 5866  =  105 bp
AmpR promoter
5762 .. 5866  =  105 bp
MCS
1360 .. 1391  =  32 bp
multiple cloning site
MCS
1360 .. 1391  =  32 bp
multiple cloning site
SV40 ori
2386 .. 2521  =  136 bp
SV40 origin of replication
SV40 ori
2386 .. 2521  =  136 bp
SV40 origin of replication
Kozak sequence
796 .. 805  =  10 bp
Kozak consensus sequence for GLuc
Kozak sequence
796 .. 805  =  10 bp
Kozak consensus sequence for GLuc
ORF:  343 .. 1359  =  1017 bp
ORF:  338 amino acids  =  37.0 kDa
ORF:  1714 .. 1980  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  2602 .. 3396  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  2774 .. 3160  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  5031 .. 5297  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  105 .. 1070  =  966 bp
ORF:  321 amino acids  =  34.6 kDa
ORF:  4901 .. 5761  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  652 .. 1386  =  735 bp
ORF:  244 amino acids  =  26.4 kDa
ORF:  2911 .. 3447  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
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Individual Sequences & Maps

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pGLuc Mini-TK 2
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pGL4.27[luc2P minP Hygro]
pGLuc-Basic 2
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pGL4.28[luc2CP minP Hygro]
phRG-B
pSF-CMV-FLuc
pCBR-Basic
pGL4.29[luc2P CRE Hygro]
phRG-TK
pSF-CMV-FMDV-FLuc
pCBR-Control
pGL4.30[luc2P NFAT-RE Hygro]
phRL-CMV
pSF-CMV-FMDV-RLuc
pCLuc Mini-TK 2
pGL4.31[luc2P GAL4UAS Hygro]
phRL-null
pSF-CMV-PGK-FLuc
pCLuc-Basic 2
pGL4.32[luc2P NF-kB-RE Hygro]
phRL-SV40
pSF-CMV-PGK-RLuc
pCMV-CLuc 2
pGL4.33[luc2P SRE Hygro]
phRL-TK
pSF-CMV-RLuc
pCMV-Cypridina Luc
pGL4.34[luc2P SRF-RE Hygro]
phRL-TK(Int-)
pSF-CMV-RSV-FLuc AscI
pCMV-Gaussia Luc
pGL4.35[luc2P 9XGAL4UAS Hygro]
pLightSwitch_Prom
pSF-CMV-RSV-RLuc AscI
pCMV-Gaussia-Dura Luc
pGL4.36[luc2P MMTV Hygro]
pLVX-MetLuc Control
pSF-CMV-Ub-FLuc AscI
pCMV-GLuc 2
pGL4.37[luc2P ARE Hygro]
pLVX-MetLuc Reporter
pSF-CMV-Ub-RLuc AscI
pCMV-Green Renilla Luc
pGL4.38[luc2P p53 RE Hygro]
pMCS-Cypridina Luc
pSF-CMVe-FLuc
pCMV-Red Firefly Luc
pGL4.39[luc2P ATF6 RE Hygro]
pMCS-Gaussia Luc
pSF-CMVe-RLuc
pCRE-MetLuc2-Reporter
pGL4.40[luc2P MRE Hygro]
pMCS-Gaussia-Dura Luc
pSF-MinProm-FLuc
pFN26A (BIND) hRluc-neo Flexi Vector
pGL4.41[luc2P HSE Hygro]
pMCS-Green Renilla Luc
pSF-MinProm-RLuc
pGEM-luc
pGL4.42[luc2P HRE Hygro]
pMCS-Red Firefly Luc
pSF-pA-CMVe-FLuc
pGL2-Basic
pGL4.43[luc2P XRE Hygro]
pMetLuc-Mem Control
pSF-pA-CMVe-RLuc
pGL2-Control
pGL4.44[luc2P AP1 RE Hygro]
pMetLuc-Mem Reporter
pSF-pA-MinProm-FLuc
pGL2-Enhancer
pGL4.45[luc2P ISRE Hygro]
pMetLuc2-Control
pSF-pA-MinProm-RLuc
pGL2-Promoter
pGL4.47[luc2P SIE Hygro]
pMetLuc2-Reporter
pSF-pA-PromMCS-FLuc
pGL3-Basic
pGL4.48[luc2P SBE Hygro]
pmirGLO
pSF-pA-PromMCS-RLuc
pGL3-Control
pGL4.49[luc2P TCF-LEF RE Hygro]
pMirTarget
pSF-PromMCS-FLuc
pGL3-Enhancer
pGL4.50[luc2 CMV Hygro]
pNFkB-MetLuc2-Reporter
pSF-PromMCS-RLuc
pGL3-Promoter
pGL4.51[luc2 CMV Neo]
pNL1.1.CMV[Nluc CMV]
pSF-RecA-FLuc
pGL4.10[luc2]
pGL4.52[luc2P STAT5 RE Hygro]
pNL1.1[Nluc]
pSP-luc+NF
pGL4.11[luc2P]
pGL4.70[hRluc]
pNL1.2[NlucP]
pSV40-CLuc
pGL4.12[luc2CP]
pGL4.71[hRlucP]
pNL1.3.CMV[secNluc CMV]
pSV40-GLuc
pGL4.13[luc2 SV40]
pGL4.72[hRlucCP]
pNL1.3[secNluc]
pTK-CLuc
pGL4.14[luc2 Hygro]
pGL4.73[hRluc SV40]
pNL2.1[Nluc Hygro]
pTK-Cypridina Luc
pGL4.15[luc2P Hygro]
pGL4.74[hRluc TK]
pNL2.2[NlucP Hygro]
pTK-Gaussia Luc
pGL4.16[luc2CP Hygro]
pGL4.75[hRluc CMV]
pNL2.3[secNluc Hygro]
pTK-Gaussia-Dura Luc
pGL4.17[luc2 Neo]
pGL4.76[hRluc Hygro]
pNL3.1[Nluc minP]
pTK-GLuc
pGL4.18[luc2P Neo]
pGL4.77[hRlucP Hygro]
pNL3.2.NF-kB-RE[NlucP NF-kB-RE Hygro]
pTK-Green Renilla Luc
pGL4.19[luc2CP Neo]
pGL4.78[hRlucCP Hygro]
pNL3.2[NlucP minP]
pTK-Red Firefly Luc
pGL4.20[luc2 Puro]
pGL4.79[hRluc Neo]
pNL3.3[secNluc minP]
pXPG
pGL4.21[luc2P Puro]
pGL4.80[hRlucP Neo]
pRL-CMV

Plasmid Sets

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I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
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Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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