pXPG

Improved high-copy luciferase plasmid, for expressing cytoplasmic luciferase as a transcriptional reporter.
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BamHI BsmBI - Esp3I PflFI - Tth111I BsaAI BstZ17I BstAPI NdeI PciI AlwNI AhdI BsaI BglI NmeAIII FspI PvuI EaeI XmnI AatII ZraI EcoRI SwaI BglII PaeR7I - XhoI Eco53kI SacI Acc65I KpnI - TspMI - XmaI SmaI SalI HindIII NcoI KasI NarI SfoI PluTI BstBI BsrGI BclI * SphI PpuMI PaqCI SgrAI XbaI PflMI BsgI BsaBI * BspDI * - ClaI * AleI PspOMI ApaI pXPG 6087 bp
BamHI  (1)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BsmBI  (5578)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (5578)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
PflFI  (5477)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (5477)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsaAI  (5471)
1 site		 Y A C G T R R T G C A Y
BstZ17I  (5452)
1 site		 G T A T A C C A T A T G
BstAPI  (5404)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
NdeI  (5400)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
PciI  (5221)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
AlwNI  (4812)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
AhdI  (4333)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BsaI  (4267)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BglI  (4215)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
NmeAIII  (4186)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
FspI  (4110)
1 site		 T G C G C A A C G C G T
PvuI  (3964)
1 site		 C G A T C G G C T A G C
EaeI  (3940)
1 site		 Y G G C C R R C C G G Y
XmnI  (3733)
1 site		 G A A N N N N T T C C T T N N N N A A G
AatII  (3414)
1 site		 G A C G T C C T G C A G
ZraI  (3412)
1 site		 G A C G T C C T G C A G
EcoRI  (3335)
1 site		 G A A T T C C T T A A G
SwaI  (3321)
1 site		 A T T T A A A T T A A A T T T A
SwaI is typically used at 25°C, but is 50% active at 37°C.
BglII  (13)
1 site		 A G A T C T T C T A G A
PaeR7I  (17)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (17)
1 site		 C T C G A G G A G C T C
Eco53kI  (22)
1 site		 G A G C T C C T C G A G
SacI  (24)
1 site		 G A G C T C C T C G A G
Acc65I  (26)
1 site		 G G T A C C C C A T G G
KpnI  (30)
1 site		 G G T A C C C C A T G G
TspMI  (30)
1 site		 C C C G G G G G G C C C
XmaI  (30)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (32)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
SalI  (35)
1 site		 G T C G A C C A G C T G
HindIII  (41)
1 site		 A A G C T T T T C G A A
NcoI  (74)
1 site		 C C A T G G G G T A C C
KasI  (108)
1 site		 G G C G C C C C G C G G
NarI  (109)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (110)
1 site		 G G C G C C C C G C G G
PluTI  (112)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BstBI  (245)
1 site		 T T C G A A A A G C T T
BsrGI  (566)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
BclI  (656)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
SphI  (739)
1 site		 G C A T G C C G T A C G
PpuMI  (1255)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
PaqCI  (1465)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
SgrAI  (1504)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
XbaI  (1730)
1 site		 T C T A G A A G A T C T
PflMI  (1888)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
BsgI  (2120)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaBI  (2351)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BspDI  (2583)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2583)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
AleI  (2783)
1 site		 C A C N N N N G T G G T G N N N N C A C
PspOMI  (2859)
1 site		 G G G C C C C C C G G G
ApaI  (2863)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
luciferase
76 .. 1728  =  1653 bp
550 amino acids  =  60.6 kDa
Product: firefly luciferase
enhanced luc+ version of the luciferase gene
luciferase
76 .. 1728  =  1653 bp
550 amino acids  =  60.6 kDa
Product: firefly luciferase
enhanced luc+ version of the luciferase gene
AmpR
3546 .. 4406  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   3546 .. 3614  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3546 .. 4406  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   3615 .. 4406  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3546 .. 4406  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
4577 .. 5165  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4577 .. 5165  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
SV40 poly(A) signal
2450 .. 2584  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2450 .. 2584  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
5730 .. 5864  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
5730 .. 5864  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
5958 .. 5  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
5958 .. 5  =  135 bp
SV40 polyadenylation signal
AmpR promoter
3441 .. 3545  =  105 bp
AmpR promoter
3441 .. 3545  =  105 bp
small t intron
1813 .. 1878  =  66 bp
simian virus 40 (SV40) small t antigen intron
small t intron
1813 .. 1878  =  66 bp
simian virus 40 (SV40) small t antigen intron
MCS
1 .. 46  =  46 bp
multiple cloning site
MCS
1 .. 46  =  46 bp
multiple cloning site
ORF:  1957 .. 2430  =  474 bp
ORF:  157 amino acids  =  18.2 kDa
ORF:  5944 .. 1728  =  1872 bp
ORF:  623 amino acids  =  68.8 kDa
ORF:  4604 .. 4831  =  228 bp
ORF:  75 amino acids  =  8.0 kDa
ORF:  2436 .. 2693  =  258 bp
ORF:  85 amino acids  =  9.6 kDa
ORF:  3546 .. 4406  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
ORF:  6067 .. 213  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
ORF:  361 .. 588  =  228 bp
ORF:  75 amino acids  =  9.1 kDa
ORF:  2559 .. 3290  =  732 bp
ORF:  243 amino acids  =  27.0 kDa
ORF:  2729 .. 3013  =  285 bp
ORF:  94 amino acids  =  10.5 kDa
ORF:  4010 .. 4276  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
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Download pXPG.dna file

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Individual Sequences & Maps

pBI-CMV5
pGL4.22[luc2CP Puro]
pGL4.81[hRlucCP Neo]
pRL-null
pBIND-ER(alpha)
pGL4.23[luc2 minP]
pGL4.82[hRluc Puro]
pRL-SV40
pBIND-GR
pGL4.24[luc2P minP]
pGL4.83[hRlucP Puro]
pRL-TK
pCBG68-Basic
pGL4.25[luc2CP minP]
pGL4.84[hRlucCP Puro]
pSELECT-zeo-Lucia
pCBG68-Control
pGL4.26[luc2 minP Hygro]
pGLuc Mini-TK 2
pSF-CMV-EMCV-FLuc
pCBG99-Basic
pGL4.27[luc2P minP Hygro]
pGLuc-Basic 2
pSF-CMV-EMCV-RLuc
pCBG99-Control
pGL4.28[luc2CP minP Hygro]
phRG-B
pSF-CMV-FLuc
pCBR-Basic
pGL4.29[luc2P CRE Hygro]
phRG-TK
pSF-CMV-FMDV-FLuc
pCBR-Control
pGL4.30[luc2P NFAT-RE Hygro]
phRL-CMV
pSF-CMV-FMDV-RLuc
pCLuc Mini-TK 2
pGL4.31[luc2P GAL4UAS Hygro]
phRL-null
pSF-CMV-PGK-FLuc
pCLuc-Basic 2
pGL4.32[luc2P NF-kB-RE Hygro]
phRL-SV40
pSF-CMV-PGK-RLuc
pCMV-CLuc 2
pGL4.33[luc2P SRE Hygro]
phRL-TK
pSF-CMV-RLuc
pCMV-Cypridina Luc
pGL4.34[luc2P SRF-RE Hygro]
phRL-TK(Int-)
pSF-CMV-RSV-FLuc AscI
pCMV-Gaussia Luc
pGL4.35[luc2P 9XGAL4UAS Hygro]
pLightSwitch_Prom
pSF-CMV-RSV-RLuc AscI
pCMV-Gaussia-Dura Luc
pGL4.36[luc2P MMTV Hygro]
pLVX-MetLuc Control
pSF-CMV-Ub-FLuc AscI
pCMV-GLuc 2
pGL4.37[luc2P ARE Hygro]
pLVX-MetLuc Reporter
pSF-CMV-Ub-RLuc AscI
pCMV-Green Renilla Luc
pGL4.38[luc2P p53 RE Hygro]
pMCS-Cypridina Luc
pSF-CMVe-FLuc
pCMV-Red Firefly Luc
pGL4.39[luc2P ATF6 RE Hygro]
pMCS-Gaussia Luc
pSF-CMVe-RLuc
pCRE-MetLuc2-Reporter
pGL4.40[luc2P MRE Hygro]
pMCS-Gaussia-Dura Luc
pSF-MinProm-FLuc
pFN26A (BIND) hRluc-neo Flexi Vector
pGL4.41[luc2P HSE Hygro]
pMCS-Green Renilla Luc
pSF-MinProm-RLuc
pGEM-luc
pGL4.42[luc2P HRE Hygro]
pMCS-Red Firefly Luc
pSF-pA-CMVe-FLuc
pGL2-Basic
pGL4.43[luc2P XRE Hygro]
pMetLuc-Mem Control
pSF-pA-CMVe-RLuc
pGL2-Control
pGL4.44[luc2P AP1 RE Hygro]
pMetLuc-Mem Reporter
pSF-pA-MinProm-FLuc
pGL2-Enhancer
pGL4.45[luc2P ISRE Hygro]
pMetLuc2-Control
pSF-pA-MinProm-RLuc
pGL2-Promoter
pGL4.47[luc2P SIE Hygro]
pMetLuc2-Reporter
pSF-pA-PromMCS-FLuc
pGL3-Basic
pGL4.48[luc2P SBE Hygro]
pmirGLO
pSF-pA-PromMCS-RLuc
pGL3-Control
pGL4.49[luc2P TCF-LEF RE Hygro]
pMirTarget
pSF-PromMCS-FLuc
pGL3-Enhancer
pGL4.50[luc2 CMV Hygro]
pNFkB-MetLuc2-Reporter
pSF-PromMCS-RLuc
pGL3-Promoter
pGL4.51[luc2 CMV Neo]
pNL1.1.CMV[Nluc CMV]
pSF-RecA-FLuc
pGL4.10[luc2]
pGL4.52[luc2P STAT5 RE Hygro]
pNL1.1[Nluc]
pSP-luc+NF
pGL4.11[luc2P]
pGL4.70[hRluc]
pNL1.2[NlucP]
pSV40-CLuc
pGL4.12[luc2CP]
pGL4.71[hRlucP]
pNL1.3.CMV[secNluc CMV]
pSV40-GLuc
pGL4.13[luc2 SV40]
pGL4.72[hRlucCP]
pNL1.3[secNluc]
pTK-CLuc
pGL4.14[luc2 Hygro]
pGL4.73[hRluc SV40]
pNL2.1[Nluc Hygro]
pTK-Cypridina Luc
pGL4.15[luc2P Hygro]
pGL4.74[hRluc TK]
pNL2.2[NlucP Hygro]
pTK-Gaussia Luc
pGL4.16[luc2CP Hygro]
pGL4.75[hRluc CMV]
pNL2.3[secNluc Hygro]
pTK-Gaussia-Dura Luc
pGL4.17[luc2 Neo]
pGL4.76[hRluc Hygro]
pNL3.1[Nluc minP]
pTK-GLuc
pGL4.18[luc2P Neo]
pGL4.77[hRlucP Hygro]
pNL3.2.NF-kB-RE[NlucP NF-kB-RE Hygro]
pTK-Green Renilla Luc
pGL4.19[luc2CP Neo]
pGL4.78[hRlucCP Hygro]
pNL3.2[NlucP minP]
pTK-Red Firefly Luc
pGL4.20[luc2 Puro]
pGL4.79[hRluc Neo]
pNL3.3[secNluc minP]
pXPG
pGL4.21[luc2P Puro]
pGL4.80[hRlucP Neo]
pRL-CMV

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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