pLVX-MetLuc Control

Lentiviral vector for constitutive expression of secreted Metridia luciferase.

Sequence Author: Clontech (TaKaRa)

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HomePlasmidsLuciferase VectorspLVX-MetLuc Control
SgrDI (8401) SspI (8284) PvuI (7850) FspI (7702) DrdI (6695) AflIII - PciI (6587) Bsu36I (5409) PflMI (4930) CsiI - SexAI * (4643) BstEII (4210) NruI * (833) FseI (1150) MfeI (1189) BbvCI (1424) KflI (1934) cPPT/CTS BspDI - ClaI (2180) NdeI (2374) SnaBI (2480) AfeI (2798) PaeR7I - XhoI (2815) BstBI (2829) EcoRI (2831) PspOMI (2855) ApaI (2859) BamHI (2862) BclI * (3040) XcmI (3080) BsrGI (3241) PaqCI (3244) SphI (3595) AgeI (3666) SpeI (3801) BlpI (3829) PasI (4125) BsiWI (4132) RsrII (4192) pLVX-MetLuc Control 8721 bp
SgrDI  (8401)
1 site		 C G T C G A C G G C A G C T G C
SspI  (8284)
1 site		 A A T A T T T T A T A A
PvuI  (7850)
1 site		 C G A T C G G C T A G C
FspI  (7702)
1 site		 T G C G C A A C G C G T
DrdI  (6695)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
AflIII  (6587)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (6587)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
Bsu36I  (5409)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
PflMI  (4930)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
CsiI  (4643)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (4643)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BstEII  (4210)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
NruI  (833)
1 site		 T C G C G A A G C G C T
* Blocked by Dam methylation.
FseI  (1150)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
MfeI  (1189)
1 site		 C A A T T G G T T A A C
BbvCI  (1424)
1 site		 C C T C A G C G G A G T C G
KflI  (1934)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different KflI sites may not be compatible.
BspDI  (2180)
1 site		 A T C G A T T A G C T A
ClaI  (2180)
1 site		 A T C G A T T A G C T A
NdeI  (2374)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (2480)
1 site		 T A C G T A A T G C A T
AfeI  (2798)
1 site		 A G C G C T T C G C G A
PaeR7I  (2815)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2815)
1 site		 C T C G A G G A G C T C
BstBI  (2829)
1 site		 T T C G A A A A G C T T
EcoRI  (2831)
1 site		 G A A T T C C T T A A G
PspOMI  (2855)
1 site		 G G G C C C C C C G G G
ApaI  (2859)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
BamHI  (2862)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BclI  (3040)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
XcmI  (3080)
1 site		 C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C
The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BsrGI  (3241)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
PaqCI  (3244)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
SphI  (3595)
1 site		 G C A T G C C G T A C G
AgeI  (3666)
1 site		 A C C G G T T G G C C A
SpeI  (3801)
1 site		 A C T A G T T G A T C A
BlpI  (3829)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
PasI  (4125)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
BsiWI  (4132)
1 site		 C G T A C G G C A T G C
BsiWI is typically used at 55°C, but is 50% active at 37°C.
RsrII  (4192)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AmpR
7407 .. 8267  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   7407 .. 8198  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7407 .. 8267  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   8199 .. 8267  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7407 .. 8267  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
MetLuc
2880 .. 3539  =  660 bp
219 amino acids  =  23.9 kDa
2 segments
   Segment 1:  signal sequence  
   2880 .. 2930  =  51 bp
   17 amino acids  =  1.9 kDa
Product: secreted Metridia luciferase
human codon-optimized
MetLuc
2880 .. 3539  =  660 bp
219 amino acids  =  23.9 kDa
2 segments
   Segment 2:  
   2931 .. 3539  =  609 bp
   202 amino acids  =  22.0 kDa
Product: secreted Metridia luciferase
human codon-optimized
MetLuc
2880 .. 3539  =  660 bp
219 amino acids  =  23.9 kDa
2 segments
Product: secreted Metridia luciferase
human codon-optimized
3' LTR
1 .. 634  =  634 bp
3' long terminal repeat (LTR) from HIV-1
3' LTR
1 .. 634  =  634 bp
3' long terminal repeat (LTR) from HIV-1
3' LTR
5484 .. 6117  =  634 bp
3' long terminal repeat (LTR) from HIV-1
3' LTR
5484 .. 6117  =  634 bp
3' long terminal repeat (LTR) from HIV-1
PuroR
4076 .. 4675  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
4076 .. 4675  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
WPRE
4689 .. 5277  =  589 bp
woodchuck posttranscriptional regulatory element
WPRE
4689 .. 5277  =  589 bp
woodchuck posttranscriptional regulatory element
ori
6648 .. 7236  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
6648 .. 7236  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
PGK promoter
3556 .. 4055  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
3556 .. 4055  =  500 bp
mouse phosphoglycerate kinase 1 promoter
CMV enhancer
2201 .. 2504  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2201 .. 2504  =  304 bp
human cytomegalovirus immediate early enhancer
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
RRE
1303 .. 1536  =  234 bp
The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.
CMV promoter
2505 .. 2708  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
2505 .. 2708  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
8420 .. 8554  =  135 bp
SV40 polyadenylation signal
SV40 poly(A) signal
8420 .. 8554  =  135 bp
SV40 polyadenylation signal
HIV-1 Ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
HIV-1 Ψ
681 .. 806  =  126 bp
packaging signal of human immunodeficiency virus type 1
cPPT/CTS
2028 .. 2144  =  117 bp
central polypurine tract and central termination sequence of HIV-1 (lacking the first T)
cPPT/CTS
2028 .. 2144  =  117 bp
central polypurine tract and central termination sequence of HIV-1 (lacking the first T)
AmpR promoter
8268 .. 8372  =  105 bp
AmpR promoter
8268 .. 8372  =  105 bp
ORF:  4792 .. 5088  =  297 bp
ORF:  98 amino acids  =  10.2 kDa
ORF:  7537 .. 7803  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  1181 .. 2032  =  852 bp
ORF:  283 amino acids  =  32.7 kDa
ORF:  3836 .. 4675  =  840 bp
ORF:  279 amino acids  =  30.1 kDa
ORF:  2880 .. 3539  =  660 bp
ORF:  219 amino acids  =  23.9 kDa
ORF:  4779 .. 5306  =  528 bp
ORF:  175 amino acids  =  19.0 kDa
ORF:  1015 .. 1476  =  462 bp
ORF:  153 amino acids  =  16.6 kDa
ORF:  7407 .. 8267  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  2822 .. 3247  =  426 bp
ORF:  141 amino acids  =  15.2 kDa
ORF:  3266 .. 3523  =  258 bp
ORF:  85 amino acids  =  9.4 kDa
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Download pLVX-MetLuc Control.dna file

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Individual Sequences & Maps

pBI-CMV5
pGL4.22[luc2CP Puro]
pGL4.81[hRlucCP Neo]
pRL-null
pBIND-ER(alpha)
pGL4.23[luc2 minP]
pGL4.82[hRluc Puro]
pRL-SV40
pBIND-GR
pGL4.24[luc2P minP]
pGL4.83[hRlucP Puro]
pRL-TK
pCBG68-Basic
pGL4.25[luc2CP minP]
pGL4.84[hRlucCP Puro]
pSELECT-zeo-Lucia
pCBG68-Control
pGL4.26[luc2 minP Hygro]
pGLuc Mini-TK 2
pSF-CMV-EMCV-FLuc
pCBG99-Basic
pGL4.27[luc2P minP Hygro]
pGLuc-Basic 2
pSF-CMV-EMCV-RLuc
pCBG99-Control
pGL4.28[luc2CP minP Hygro]
phRG-B
pSF-CMV-FLuc
pCBR-Basic
pGL4.29[luc2P CRE Hygro]
phRG-TK
pSF-CMV-FMDV-FLuc
pCBR-Control
pGL4.30[luc2P NFAT-RE Hygro]
phRL-CMV
pSF-CMV-FMDV-RLuc
pCLuc Mini-TK 2
pGL4.31[luc2P GAL4UAS Hygro]
phRL-null
pSF-CMV-PGK-FLuc
pCLuc-Basic 2
pGL4.32[luc2P NF-kB-RE Hygro]
phRL-SV40
pSF-CMV-PGK-RLuc
pCMV-CLuc 2
pGL4.33[luc2P SRE Hygro]
phRL-TK
pSF-CMV-RLuc
pCMV-Cypridina Luc
pGL4.34[luc2P SRF-RE Hygro]
phRL-TK(Int-)
pSF-CMV-RSV-FLuc AscI
pCMV-Gaussia Luc
pGL4.35[luc2P 9XGAL4UAS Hygro]
pLightSwitch_Prom
pSF-CMV-RSV-RLuc AscI
pCMV-Gaussia-Dura Luc
pGL4.36[luc2P MMTV Hygro]
pLVX-MetLuc Control
pSF-CMV-Ub-FLuc AscI
pCMV-GLuc 2
pGL4.37[luc2P ARE Hygro]
pLVX-MetLuc Reporter
pSF-CMV-Ub-RLuc AscI
pCMV-Green Renilla Luc
pGL4.38[luc2P p53 RE Hygro]
pMCS-Cypridina Luc
pSF-CMVe-FLuc
pCMV-Red Firefly Luc
pGL4.39[luc2P ATF6 RE Hygro]
pMCS-Gaussia Luc
pSF-CMVe-RLuc
pCRE-MetLuc2-Reporter
pGL4.40[luc2P MRE Hygro]
pMCS-Gaussia-Dura Luc
pSF-MinProm-FLuc
pFN26A (BIND) hRluc-neo Flexi Vector
pGL4.41[luc2P HSE Hygro]
pMCS-Green Renilla Luc
pSF-MinProm-RLuc
pGEM-luc
pGL4.42[luc2P HRE Hygro]
pMCS-Red Firefly Luc
pSF-pA-CMVe-FLuc
pGL2-Basic
pGL4.43[luc2P XRE Hygro]
pMetLuc-Mem Control
pSF-pA-CMVe-RLuc
pGL2-Control
pGL4.44[luc2P AP1 RE Hygro]
pMetLuc-Mem Reporter
pSF-pA-MinProm-FLuc
pGL2-Enhancer
pGL4.45[luc2P ISRE Hygro]
pMetLuc2-Control
pSF-pA-MinProm-RLuc
pGL2-Promoter
pGL4.47[luc2P SIE Hygro]
pMetLuc2-Reporter
pSF-pA-PromMCS-FLuc
pGL3-Basic
pGL4.48[luc2P SBE Hygro]
pmirGLO
pSF-pA-PromMCS-RLuc
pGL3-Control
pGL4.49[luc2P TCF-LEF RE Hygro]
pMirTarget
pSF-PromMCS-FLuc
pGL3-Enhancer
pGL4.50[luc2 CMV Hygro]
pNFkB-MetLuc2-Reporter
pSF-PromMCS-RLuc
pGL3-Promoter
pGL4.51[luc2 CMV Neo]
pNL1.1.CMV[Nluc CMV]
pSF-RecA-FLuc
pGL4.10[luc2]
pGL4.52[luc2P STAT5 RE Hygro]
pNL1.1[Nluc]
pSP-luc+NF
pGL4.11[luc2P]
pGL4.70[hRluc]
pNL1.2[NlucP]
pSV40-CLuc
pGL4.12[luc2CP]
pGL4.71[hRlucP]
pNL1.3.CMV[secNluc CMV]
pSV40-GLuc
pGL4.13[luc2 SV40]
pGL4.72[hRlucCP]
pNL1.3[secNluc]
pTK-CLuc
pGL4.14[luc2 Hygro]
pGL4.73[hRluc SV40]
pNL2.1[Nluc Hygro]
pTK-Cypridina Luc
pGL4.15[luc2P Hygro]
pGL4.74[hRluc TK]
pNL2.2[NlucP Hygro]
pTK-Gaussia Luc
pGL4.16[luc2CP Hygro]
pGL4.75[hRluc CMV]
pNL2.3[secNluc Hygro]
pTK-Gaussia-Dura Luc
pGL4.17[luc2 Neo]
pGL4.76[hRluc Hygro]
pNL3.1[Nluc minP]
pTK-GLuc
pGL4.18[luc2P Neo]
pGL4.77[hRlucP Hygro]
pNL3.2.NF-kB-RE[NlucP NF-kB-RE Hygro]
pTK-Green Renilla Luc
pGL4.19[luc2CP Neo]
pGL4.78[hRlucCP Hygro]
pNL3.2[NlucP minP]
pTK-Red Firefly Luc
pGL4.20[luc2 Puro]
pGL4.79[hRluc Neo]
pNL3.3[secNluc minP]
pXPG
pGL4.21[luc2P Puro]
pGL4.80[hRlucP Neo]
pRL-CMV

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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