pSELECT-zeo-Lucia

Mammalian vector for the expression of secreted Lucia® luciferase.

Sequence Author: InvivoGen

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HomePlasmidsLuciferase VectorspSELECT-zeo-Lucia
NotI (2) BciVI (3224) BssSI - BssSαI (3194) DrdI (3129) NspI (3025) AflIII - PciI (3021) PstI - SbfI (3008) NdeI (2826) BsaAI - SnaBI (2722) SpeI (2592) SacI (2498) Eco53kI (2496) BsmBI - Esp3I (2469) AseI (2438) SfiI (2384) BssHII - MauBI (2341) BsrBI (2327) SmaI (2291) TspMI - XmaI (2289) SgrAI (2263) BmgBI (2247) CsiI - SexAI * (2213) NmeAIII (2133) FseI (2109) DraIII (2031) BseRI (2008) AsiSI - PvuI (176) AclI (370) PvuII (407) HindIII (411) KasI (701) NarI (702) SfoI (703) PluTI (705) AgeI (718) BstEII (721) NcoI (726) BbvCI (771) BbsI (804) BglII (841) EcoRV (1138) NheI (1360) BmtI (1364) BtsI - BtsαI (1426) PsiI (1480) HpaI (1500) EcoRI (1594) BspQI - EarI - SapI (1783) SspI (1835) pSELECT-zeo-Lucia 3769 bp
NotI  (2)
1 site		 G C G G C C G C C G C C G G C G
BciVI  (3224)
1 site		 G T A T C C ( N ) 5 N C A T A G G ( N ) 5
The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
BssSI  (3194)
1 site		 C A C G A G G T G C T C
BssSαI  (3194)
1 site		 C A C G A G G T G C T C
DrdI  (3129)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
NspI  (3025)
1 site		 R C A T G Y Y G T A C R
AflIII  (3021)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (3021)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
PstI  (3008)
1 site		 C T G C A G G A C G T C
SbfI  (3008)
1 site		 C C T G C A G G G G A C G T C C
NdeI  (2826)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BsaAI  (2722)
1 site		 Y A C G T R R T G C A Y
SnaBI  (2722)
1 site		 T A C G T A A T G C A T
SpeI  (2592)
1 site		 A C T A G T T G A T C A
SacI  (2498)
1 site		 G A G C T C C T C G A G
Eco53kI  (2496)
1 site		 G A G C T C C T C G A G
BsmBI  (2469)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (2469)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
AseI  (2438)
1 site		 A T T A A T T A A T T A
SfiI  (2384)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BssHII  (2341)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
MauBI  (2341)
1 site		 C G C G C G C G G C G C G C G C
BsrBI  (2327)
1 site		 C C G C T C G G C G A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
SmaI  (2291)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
TspMI  (2289)
1 site		 C C C G G G G G G C C C
XmaI  (2289)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SgrAI  (2263)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BmgBI  (2247)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
CsiI  (2213)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (2213)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
NmeAIII  (2133)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
FseI  (2109)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
DraIII  (2031)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
BseRI  (2008)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
AsiSI  (176)
1 site		 G C G A T C G C C G C T A G C G
PvuI  (176)
1 site		 C G A T C G G C T A G C
AclI  (370)
1 site		 A A C G T T T T G C A A
PvuII  (407)
1 site		 C A G C T G G T C G A C
HindIII  (411)
1 site		 A A G C T T T T C G A A
KasI  (701)
1 site		 G G C G C C C C G C G G
NarI  (702)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (703)
1 site		 G G C G C C C C G C G G
PluTI  (705)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
AgeI  (718)
1 site		 A C C G G T T G G C C A
BstEII  (721)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
NcoI  (726)
1 site		 C C A T G G G G T A C C
BbvCI  (771)
1 site		 C C T C A G C G G A G T C G
BbsI  (804)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BglII  (841)
1 site		 A G A T C T T C T A G A
EcoRV  (1138)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
NheI  (1360)
1 site		 G C T A G C C G A T C G
BmtI  (1364)
1 site		 G C T A G C C G A T C G
BtsI  (1426)
1 site		 G C A G T G N N C G T C A C
BtsαI  (1426)
1 site		 G C A G T G N N C G T C A C
Sticky ends from different BtsαI sites may not be compatible.
PsiI  (1480)
1 site		 T T A T A A A A T A T T
HpaI  (1500)
1 site		 G T T A A C C A A T T G
EcoRI  (1594)
1 site		 G A A T T C C T T A A G
BspQI  (1783)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
EarI  (1783)
1 site		 C T C T T C N G A G A A G N N N N
Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
SapI  (1783)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
SspI  (1835)
1 site		 A A T A T T T T A T A A
Lucia®
728 .. 1357  =  630 bp
209 amino acids  =  22.8 kDa
Product: secreted coelenterazine-utilizing luciferase
synthetic gene based on luciferases from marine copepods
Lucia®
728 .. 1357  =  630 bp
209 amino acids  =  22.8 kDa
Product: secreted coelenterazine-utilizing luciferase
synthetic gene based on luciferases from marine copepods
ori
3082 .. 3670  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3082 .. 3670  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
EF-1α/HTLV composite promoter
178 .. 688  =  511 bp
3 segments
   Segment 1:  EF-1α segment  
   178 .. 407  =  230 bp
EF-1α/HTLV composite promoter
178 .. 688  =  511 bp
3 segments
   Segment 2:  
   408 .. 419  =  12 bp
EF-1α/HTLV composite promoter
178 .. 688  =  511 bp
3 segments
   Segment 3:  HTLV segment  
   420 .. 688  =  269 bp
EF-1α/HTLV composite promoter
178 .. 688  =  511 bp
3 segments
β-globin poly(A)
1600 .. 1994  =  395 bp
human β-globin polyadenylation signal
β-globin poly(A)
1600 .. 1994  =  395 bp
human β-globin polyadenylation signal
BleoR
2008 .. 2382  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
BleoR
2008 .. 2382  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and Zeocin™
CMV enhancer
2699 .. 3002  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
2699 .. 3002  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
2495 .. 2698  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
2495 .. 2698  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
SV40 poly(A) signal
1379 .. 1500  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1379 .. 1500  =  122 bp
SV40 polyadenylation signal
pause site
68 .. 159  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
pause site
68 .. 159  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
poly(A) signal
9 .. 57  =  49 bp
synthetic polyadenylation signal
poly(A) signal
9 .. 57  =  49 bp
synthetic polyadenylation signal
T7 promoter
2392 .. 2410  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
2392 .. 2410  =  19 bp
promoter for bacteriophage T7 RNA polymerase
ORF:  728 .. 1357  =  630 bp
ORF:  209 amino acids  =  22.8 kDa
ORF:  2102 .. 2398  =  297 bp
ORF:  98 amino acids  =  10.4 kDa
ORF:  706 .. 1035  =  330 bp
ORF:  109 amino acids  =  11.5 kDa
ORF:  2008 .. 2382  =  375 bp
ORF:  124 amino acids  =  13.8 kDa
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Individual Sequences & Maps

pBI-CMV5
pGL4.22[luc2CP Puro]
pGL4.81[hRlucCP Neo]
pRL-null
pBIND-ER(alpha)
pGL4.23[luc2 minP]
pGL4.82[hRluc Puro]
pRL-SV40
pBIND-GR
pGL4.24[luc2P minP]
pGL4.83[hRlucP Puro]
pRL-TK
pCBG68-Basic
pGL4.25[luc2CP minP]
pGL4.84[hRlucCP Puro]
pSELECT-zeo-Lucia
pCBG68-Control
pGL4.26[luc2 minP Hygro]
pGLuc Mini-TK 2
pSF-CMV-EMCV-FLuc
pCBG99-Basic
pGL4.27[luc2P minP Hygro]
pGLuc-Basic 2
pSF-CMV-EMCV-RLuc
pCBG99-Control
pGL4.28[luc2CP minP Hygro]
phRG-B
pSF-CMV-FLuc
pCBR-Basic
pGL4.29[luc2P CRE Hygro]
phRG-TK
pSF-CMV-FMDV-FLuc
pCBR-Control
pGL4.30[luc2P NFAT-RE Hygro]
phRL-CMV
pSF-CMV-FMDV-RLuc
pCLuc Mini-TK 2
pGL4.31[luc2P GAL4UAS Hygro]
phRL-null
pSF-CMV-PGK-FLuc
pCLuc-Basic 2
pGL4.32[luc2P NF-kB-RE Hygro]
phRL-SV40
pSF-CMV-PGK-RLuc
pCMV-CLuc 2
pGL4.33[luc2P SRE Hygro]
phRL-TK
pSF-CMV-RLuc
pCMV-Cypridina Luc
pGL4.34[luc2P SRF-RE Hygro]
phRL-TK(Int-)
pSF-CMV-RSV-FLuc AscI
pCMV-Gaussia Luc
pGL4.35[luc2P 9XGAL4UAS Hygro]
pLightSwitch_Prom
pSF-CMV-RSV-RLuc AscI
pCMV-Gaussia-Dura Luc
pGL4.36[luc2P MMTV Hygro]
pLVX-MetLuc Control
pSF-CMV-Ub-FLuc AscI
pCMV-GLuc 2
pGL4.37[luc2P ARE Hygro]
pLVX-MetLuc Reporter
pSF-CMV-Ub-RLuc AscI
pCMV-Green Renilla Luc
pGL4.38[luc2P p53 RE Hygro]
pMCS-Cypridina Luc
pSF-CMVe-FLuc
pCMV-Red Firefly Luc
pGL4.39[luc2P ATF6 RE Hygro]
pMCS-Gaussia Luc
pSF-CMVe-RLuc
pCRE-MetLuc2-Reporter
pGL4.40[luc2P MRE Hygro]
pMCS-Gaussia-Dura Luc
pSF-MinProm-FLuc
pFN26A (BIND) hRluc-neo Flexi Vector
pGL4.41[luc2P HSE Hygro]
pMCS-Green Renilla Luc
pSF-MinProm-RLuc
pGEM-luc
pGL4.42[luc2P HRE Hygro]
pMCS-Red Firefly Luc
pSF-pA-CMVe-FLuc
pGL2-Basic
pGL4.43[luc2P XRE Hygro]
pMetLuc-Mem Control
pSF-pA-CMVe-RLuc
pGL2-Control
pGL4.44[luc2P AP1 RE Hygro]
pMetLuc-Mem Reporter
pSF-pA-MinProm-FLuc
pGL2-Enhancer
pGL4.45[luc2P ISRE Hygro]
pMetLuc2-Control
pSF-pA-MinProm-RLuc
pGL2-Promoter
pGL4.47[luc2P SIE Hygro]
pMetLuc2-Reporter
pSF-pA-PromMCS-FLuc
pGL3-Basic
pGL4.48[luc2P SBE Hygro]
pmirGLO
pSF-pA-PromMCS-RLuc
pGL3-Control
pGL4.49[luc2P TCF-LEF RE Hygro]
pMirTarget
pSF-PromMCS-FLuc
pGL3-Enhancer
pGL4.50[luc2 CMV Hygro]
pNFkB-MetLuc2-Reporter
pSF-PromMCS-RLuc
pGL3-Promoter
pGL4.51[luc2 CMV Neo]
pNL1.1.CMV[Nluc CMV]
pSF-RecA-FLuc
pGL4.10[luc2]
pGL4.52[luc2P STAT5 RE Hygro]
pNL1.1[Nluc]
pSP-luc+NF
pGL4.11[luc2P]
pGL4.70[hRluc]
pNL1.2[NlucP]
pSV40-CLuc
pGL4.12[luc2CP]
pGL4.71[hRlucP]
pNL1.3.CMV[secNluc CMV]
pSV40-GLuc
pGL4.13[luc2 SV40]
pGL4.72[hRlucCP]
pNL1.3[secNluc]
pTK-CLuc
pGL4.14[luc2 Hygro]
pGL4.73[hRluc SV40]
pNL2.1[Nluc Hygro]
pTK-Cypridina Luc
pGL4.15[luc2P Hygro]
pGL4.74[hRluc TK]
pNL2.2[NlucP Hygro]
pTK-Gaussia Luc
pGL4.16[luc2CP Hygro]
pGL4.75[hRluc CMV]
pNL2.3[secNluc Hygro]
pTK-Gaussia-Dura Luc
pGL4.17[luc2 Neo]
pGL4.76[hRluc Hygro]
pNL3.1[Nluc minP]
pTK-GLuc
pGL4.18[luc2P Neo]
pGL4.77[hRlucP Hygro]
pNL3.2.NF-kB-RE[NlucP NF-kB-RE Hygro]
pTK-Green Renilla Luc
pGL4.19[luc2CP Neo]
pGL4.78[hRlucCP Hygro]
pNL3.2[NlucP minP]
pTK-Red Firefly Luc
pGL4.20[luc2 Puro]
pGL4.79[hRluc Neo]
pNL3.3[secNluc minP]
pXPG
pGL4.21[luc2P Puro]
pGL4.80[hRlucP Neo]
pRL-CMV

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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