pTK-CLuc

Mammalian vector with a constitutive HSV thymidine kinase promoter for expressing secreted Cypridina luciferase.

Sequence Author: New England Biolabs

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HomePlasmidsLuciferase VectorspTK-CLuc
ZraI (6997) SspI (6881) ScaI (6557) TatI (6555) PvuI (6447) AhdI (6077) RsrII (4349) StuI (3622) SgrDI (6998) AatII (6999) SacII (310) EcoRI (660) MluI (724) HindIII (771) Acc65I (777) KpnI (781) Eco53kI (785) SacI (787) BamHI (789) BsgI (932) PaqCI (954) XcmI (1199) BstEII (1310) PshAI (1543) PmlI (1697) BmgBI (1720) KflI (1809) Bsu36I (2361) NotI (2464) AgeI (2471) PaeR7I - XhoI (2477) XbaI (2489) HpaI (2631) MfeI (2640) BspDI - ClaI (2726) DraIII (3099) pTK-CLuc 7000 bp
ZraI  (6997)
1 site		 G A C G T C C T G C A G
SspI  (6881)
1 site		 A A T A T T T T A T A A
ScaI  (6557)
1 site		 A G T A C T T C A T G A
TatI  (6555)
1 site		 W G T A C W W C A T G W
PvuI  (6447)
1 site		 C G A T C G G C T A G C
AhdI  (6077)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
RsrII  (4349)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
StuI  (3622)
1 site		 A G G C C T T C C G G A
SgrDI  (6998)
1 site		 C G T C G A C G G C A G C T G C
AatII  (6999)
1 site		 G A C G T C C T G C A G
SacII  (310)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
EcoRI  (660)
1 site		 G A A T T C C T T A A G
MluI  (724)
1 site		 A C G C G T T G C G C A
HindIII  (771)
1 site		 A A G C T T T T C G A A
Acc65I  (777)
1 site		 G G T A C C C C A T G G
KpnI  (781)
1 site		 G G T A C C C C A T G G
Eco53kI  (785)
1 site		 G A G C T C C T C G A G
SacI  (787)
1 site		 G A G C T C C T C G A G
BamHI  (789)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BsgI  (932)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PaqCI  (954)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
XcmI  (1199)
1 site		 C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C
The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BstEII  (1310)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PshAI  (1543)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
PmlI  (1697)
1 site		 C A C G T G G T G C A C
BmgBI  (1720)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
KflI  (1809)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different KflI sites may not be compatible.
Bsu36I  (2361)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
NotI  (2464)
1 site		 G C G G C C G C C G C C G G C G
AgeI  (2471)
1 site		 A C C G G T T G G C C A
PaeR7I  (2477)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2477)
1 site		 C T C G A G G A G C T C
XbaI  (2489)
1 site		 T C T A G A A G A T C T
HpaI  (2631)
1 site		 G T T A A C C A A T T G
MfeI  (2640)
1 site		 C A A T T G G T T A A C
BspDI  (2726)
1 site		 A T C G A T T A G C T A
ClaI  (2726)
1 site		 A T C G A T T A G C T A
DraIII  (3099)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
CLuc
801 .. 2462  =  1662 bp
553 amino acids  =  61.5 kDa
Product: secreted Cypridina luciferase
contains 30 codon substitutions for efficient translation in mammalian cells
CLuc
801 .. 2462  =  1662 bp
553 amino acids  =  61.5 kDa
Product: secreted Cypridina luciferase
contains 30 codon substitutions for efficient translation in mammalian cells
AmpR
6004 .. 6864  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   6004 .. 6795  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6004 .. 6864  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   6796 .. 6864  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6004 .. 6864  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR
3705 .. 4499  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
3705 .. 4499  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
HSV TK promoter
19 .. 770  =  752 bp
herpes simplex virus thymidine kinase promoter
HSV TK promoter
19 .. 770  =  752 bp
herpes simplex virus thymidine kinase promoter
ori
5245 .. 5833  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5245 .. 5833  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
2866 .. 3294  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
2866 .. 3294  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SV40 promoter
3308 .. 3638  =  331 bp
SV40 enhancer and early promoter
SV40 promoter
3308 .. 3638  =  331 bp
SV40 enhancer and early promoter
SV40 poly(A) signal
2510 .. 2631  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2510 .. 2631  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
4673 .. 4794  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
4673 .. 4794  =  122 bp
SV40 polyadenylation signal
AmpR promoter
6865 .. 6969  =  105 bp
AmpR promoter
6865 .. 6969  =  105 bp
MCS
2463 .. 2494  =  32 bp
multiple cloning site
MCS
2463 .. 2494  =  32 bp
multiple cloning site
SV40 ori
3489 .. 3624  =  136 bp
SV40 origin of replication
SV40 ori
3489 .. 3624  =  136 bp
SV40 origin of replication
Kozak sequence
795 .. 804  =  10 bp
Kozak sequence
795 .. 804  =  10 bp
ORF:  343 .. 804  =  462 bp
ORF:  153 amino acids  =  17.1 kDa
ORF:  1555 .. 1866  =  312 bp
ORF:  103 amino acids  =  11.3 kDa
ORF:  3877 .. 4263  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  6134 .. 6400  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  801 .. 2462  =  1662 bp
ORF:  553 amino acids  =  61.5 kDa
ORF:  2817 .. 3083  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  3705 .. 4499  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  2170 .. 2394  =  225 bp
ORF:  74 amino acids  =  8.7 kDa
ORF:  6004 .. 6864  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  105 .. 551  =  447 bp
ORF:  148 amino acids  =  16.0 kDa
ORF:  2058 .. 2426  =  369 bp
ORF:  122 amino acids  =  13.8 kDa
ORF:  4014 .. 4550  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
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pGL4.31[luc2P GAL4UAS Hygro]
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phRL-SV40
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pGL4.33[luc2P SRE Hygro]
phRL-TK
pSF-CMV-RLuc
pCMV-Cypridina Luc
pGL4.34[luc2P SRF-RE Hygro]
phRL-TK(Int-)
pSF-CMV-RSV-FLuc AscI
pCMV-Gaussia Luc
pGL4.35[luc2P 9XGAL4UAS Hygro]
pLightSwitch_Prom
pSF-CMV-RSV-RLuc AscI
pCMV-Gaussia-Dura Luc
pGL4.36[luc2P MMTV Hygro]
pLVX-MetLuc Control
pSF-CMV-Ub-FLuc AscI
pCMV-GLuc 2
pGL4.37[luc2P ARE Hygro]
pLVX-MetLuc Reporter
pSF-CMV-Ub-RLuc AscI
pCMV-Green Renilla Luc
pGL4.38[luc2P p53 RE Hygro]
pMCS-Cypridina Luc
pSF-CMVe-FLuc
pCMV-Red Firefly Luc
pGL4.39[luc2P ATF6 RE Hygro]
pMCS-Gaussia Luc
pSF-CMVe-RLuc
pCRE-MetLuc2-Reporter
pGL4.40[luc2P MRE Hygro]
pMCS-Gaussia-Dura Luc
pSF-MinProm-FLuc
pFN26A (BIND) hRluc-neo Flexi Vector
pGL4.41[luc2P HSE Hygro]
pMCS-Green Renilla Luc
pSF-MinProm-RLuc
pGEM-luc
pGL4.42[luc2P HRE Hygro]
pMCS-Red Firefly Luc
pSF-pA-CMVe-FLuc
pGL2-Basic
pGL4.43[luc2P XRE Hygro]
pMetLuc-Mem Control
pSF-pA-CMVe-RLuc
pGL2-Control
pGL4.44[luc2P AP1 RE Hygro]
pMetLuc-Mem Reporter
pSF-pA-MinProm-FLuc
pGL2-Enhancer
pGL4.45[luc2P ISRE Hygro]
pMetLuc2-Control
pSF-pA-MinProm-RLuc
pGL2-Promoter
pGL4.47[luc2P SIE Hygro]
pMetLuc2-Reporter
pSF-pA-PromMCS-FLuc
pGL3-Basic
pGL4.48[luc2P SBE Hygro]
pmirGLO
pSF-pA-PromMCS-RLuc
pGL3-Control
pGL4.49[luc2P TCF-LEF RE Hygro]
pMirTarget
pSF-PromMCS-FLuc
pGL3-Enhancer
pGL4.50[luc2 CMV Hygro]
pNFkB-MetLuc2-Reporter
pSF-PromMCS-RLuc
pGL3-Promoter
pGL4.51[luc2 CMV Neo]
pNL1.1.CMV[Nluc CMV]
pSF-RecA-FLuc
pGL4.10[luc2]
pGL4.52[luc2P STAT5 RE Hygro]
pNL1.1[Nluc]
pSP-luc+NF
pGL4.11[luc2P]
pGL4.70[hRluc]
pNL1.2[NlucP]
pSV40-CLuc
pGL4.12[luc2CP]
pGL4.71[hRlucP]
pNL1.3.CMV[secNluc CMV]
pSV40-GLuc
pGL4.13[luc2 SV40]
pGL4.72[hRlucCP]
pNL1.3[secNluc]
pTK-CLuc
pGL4.14[luc2 Hygro]
pGL4.73[hRluc SV40]
pNL2.1[Nluc Hygro]
pTK-Cypridina Luc
pGL4.15[luc2P Hygro]
pGL4.74[hRluc TK]
pNL2.2[NlucP Hygro]
pTK-Gaussia Luc
pGL4.16[luc2CP Hygro]
pGL4.75[hRluc CMV]
pNL2.3[secNluc Hygro]
pTK-Gaussia-Dura Luc
pGL4.17[luc2 Neo]
pGL4.76[hRluc Hygro]
pNL3.1[Nluc minP]
pTK-GLuc
pGL4.18[luc2P Neo]
pGL4.77[hRlucP Hygro]
pNL3.2.NF-kB-RE[NlucP NF-kB-RE Hygro]
pTK-Green Renilla Luc
pGL4.19[luc2CP Neo]
pGL4.78[hRlucCP Hygro]
pNL3.2[NlucP minP]
pTK-Red Firefly Luc
pGL4.20[luc2 Puro]
pGL4.79[hRluc Neo]
pNL3.3[secNluc minP]
pXPG
pGL4.21[luc2P Puro]
pGL4.80[hRlucP Neo]
pRL-CMV

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