pSF-CMV-PGK-FLuc

Dual-promoter mammalian vector for strong constitutive expression of a gene together with firefly luciferase.

Sequence Author: Oxford Genetics

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HomePlasmidsLuciferase VectorspSF-CMV-PGK-FLuc
PmeI (6275) RsrII (5968) PflFI - Tth111I (5570) PmeI (5227) SanDI (5161) AscI - BssHII (5070) FseI (4924) SwaI (4818) ApaLI (4243) SwaI (3925) PacI (3799) BstEII (3773) 3' β-globin insulator PstI - SbfI (3667) MfeI (3330) AanI - PsiI (3301) BtsI (3247) stop codons AsiSI - PvuI (5) BglII (232) CMV enhancer SnaBI (566) BglII (816) EagI - NotI (858) HindIII (869) Eco53kI (879) SacI (881) EcoRI (885) KpnI (901) NcoI (905) KpnI (915) EcoRV (921) AbsI - PaeR7I - PspXI - XhoI (928) XbaI (937) BseRI - BsgI (940) stop codons BspDI - ClaI (977) SphI (1027) BsmBI - SpeI (1233) BspEI (1407) BamHI (1488) BbsI (1556) PvuII (1572) ScaI (1663) BsrGI (1988) AleI (1996) FspI (2320) DraIII (2644) SexAI * (2837) NheI (3151) BmtI (3155) pSF-CMV-PGK-FLuc 6394 bp
PmeI  (6275)
2 sites		 G T T T A A A C C A A A T T T G
RsrII  (5968)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PflFI  (5570)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (5570)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PmeI  (5227)
2 sites		 G T T T A A A C C A A A T T T G
SanDI  (5161)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different SanDI sites may not be compatible.
AscI  (5070)
1 site		 G G C G C G C C C C G C G C G G
BssHII  (5070)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
FseI  (4924)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
SwaI  (4818)
2 sites		 A T T T A A A T T A A A T T T A
SwaI is typically used at 25°C, but is 50% active at 37°C.
ApaLI  (4243)
1 site		 G T G C A C C A C G T G
SwaI  (3925)
2 sites		 A T T T A A A T T A A A T T T A
SwaI is typically used at 25°C, but is 50% active at 37°C.
PacI  (3799)
1 site		 T T A A T T A A A A T T A A T T
BstEII  (3773)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PstI  (3667)
1 site		 C T G C A G G A C G T C
SbfI  (3667)
1 site		 C C T G C A G G G G A C G T C C
MfeI  (3330)
1 site		 C A A T T G G T T A A C
AanI  (3301)
1 site		 T T A T A A A A T A T T
PsiI  (3301)
1 site		 T T A T A A A A T A T T
BtsI  (3247)
1 site		 G C A G T G N N C G T C A C
AsiSI  (5)
1 site		 G C G A T C G C C G C T A G C G
PvuI  (5)
1 site		 C G A T C G G C T A G C
BglII  (232)
2 sites		 A G A T C T T C T A G A
SnaBI  (566)
1 site		 T A C G T A A T G C A T
BglII  (816)
2 sites		 A G A T C T T C T A G A
EagI  (858)
1 site		 C G G C C G G C C G G C
NotI  (858)
1 site		 G C G G C C G C C G C C G G C G
HindIII  (869)
1 site		 A A G C T T T T C G A A
Eco53kI  (879)
1 site		 G A G C T C C T C G A G
SacI  (881)
1 site		 G A G C T C C T C G A G
EcoRI  (885)
1 site		 G A A T T C C T T A A G
KpnI  (901)
2 sites		 G G T A C C C C A T G G
NcoI  (905)
1 site		 C C A T G G G G T A C C
KpnI  (915)
2 sites		 G G T A C C C C A T G G
EcoRV  (921)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
AbsI  (928)
1 site		 C C T C G A G G G G A G C T C C
PaeR7I  (928)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (928)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (928)
1 site		 C T C G A G G A G C T C
XbaI  (937)
1 site		 T C T A G A A G A T C T
BseRI  (940)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BsgI  (940)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BspDI  (977)
1 site		 A T C G A T T A G C T A
ClaI  (977)
1 site		 A T C G A T T A G C T A
SphI  (1027)
1 site		 G C A T G C C G T A C G
BsmBI  (1233)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
SpeI  (1233)
1 site		 A C T A G T T G A T C A
BspEI  (1407)
1 site		 T C C G G A A G G C C T
BamHI  (1488)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BbsI  (1556)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
PvuII  (1572)
1 site		 C A G C T G G T C G A C
ScaI  (1663)
1 site		 A G T A C T T C A T G A
BsrGI  (1988)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
AleI  (1996)
1 site		 C A C N N N N G T G G T G N N N N C A C
FspI  (2320)
1 site		 T G C G C A A C G C G T
DraIII  (2644)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
SexAI  (2837)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
NheI  (3151)
1 site		 G C T A G C C G A T C G
BmtI  (3155)
1 site		 G C T A G C C G A T C G
luciferase
1498 .. 3150  =  1653 bp
550 amino acids  =  60.6 kDa
Product: firefly luciferase
synthetic luc2 version of the luciferase gene
luciferase
1498 .. 3150  =  1653 bp
550 amino acids  =  60.6 kDa
Product: firefly luciferase
synthetic luc2 version of the luciferase gene
NeoR/KanR
5324 .. 6118  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
5324 .. 6118  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori
3990 .. 4578  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3990 .. 4578  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
PGK promoter
988 .. 1487  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
988 .. 1487  =  500 bp
mouse phosphoglycerate kinase 1 promoter
CMV enhancer
287 .. 590  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
287 .. 590  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
591 .. 794  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
591 .. 794  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
rrnG terminator
3512 .. 3648  =  137 bp
transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)
rrnG terminator
3512 .. 3648  =  137 bp
transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)
rrnG terminator
6131 .. 6267  =  137 bp
transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)
rrnG terminator
6131 .. 6267  =  137 bp
transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)
SV40 poly(A) signal
3200 .. 3321  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3200 .. 3321  =  122 bp
SV40 polyadenylation signal
MCS
857 .. 942  =  86 bp
multiple cloning site
MCS
857 .. 942  =  86 bp
multiple cloning site
5' β-globin insulator
18 .. 89  =  72 bp
insulator upstream of the human β-globin locus (Farrell et al., 2002)
5' β-globin insulator
18 .. 89  =  72 bp
insulator upstream of the human β-globin locus (Farrell et al., 2002)
3' β-globin insulator
3693 .. 3764  =  72 bp
insulator downstream of the human β-globin locus (Farrell et al., 2002)
3' β-globin insulator
3693 .. 3764  =  72 bp
insulator downstream of the human β-globin locus (Farrell et al., 2002)
T7 terminator
3436 .. 3482  =  47 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
3436 .. 3482  =  47 bp
transcription terminator for bacteriophage T7 RNA polymerase
stop codons
961 .. 971  =  11 bp
stop codons in all three reading frames
stop codons
961 .. 971  =  11 bp
stop codons in all three reading frames
stop codons
3158 .. 3168  =  11 bp
stop codons in all three reading frames
stop codons
3158 .. 3168  =  11 bp
stop codons in all three reading frames
RBS
5311 .. 5316  =  6 bp
Shine-Dalgarno ribosome binding site
RBS
5311 .. 5316  =  6 bp
Shine-Dalgarno ribosome binding site
Kozak sequence
903 .. 909  =  7 bp
Kozak sequence
903 .. 909  =  7 bp
RBS
893 .. 898  =  6 bp
Shinbe-Dalgarno ribosome binding site
RBS
893 .. 898  =  6 bp
Shinbe-Dalgarno ribosome binding site
ORF:  1498 .. 3150  =  1653 bp
ORF:  550 amino acids  =  60.6 kDa
ORF:  1268 .. 1588  =  321 bp
ORF:  106 amino acids  =  11.5 kDa
ORF:  5324 .. 6118  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  3537 .. 3968  =  432 bp
ORF:  143 amino acids  =  16.3 kDa
ORF:  5496 .. 5882  =  387 bp
ORF:  128 amino acids  =  14.7 kDa
ORF:  6156 .. 196  =  435 bp
ORF:  144 amino acids  =  16.1 kDa
ORF:  1378 .. 1725  =  348 bp
ORF:  115 amino acids  =  12.7 kDa
ORF:  2494 .. 2754  =  261 bp
ORF:  86 amino acids  =  8.9 kDa
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Individual Sequences & Maps

pBI-CMV5
pGL4.22[luc2CP Puro]
pGL4.81[hRlucCP Neo]
pRL-null
pBIND-ER(alpha)
pGL4.23[luc2 minP]
pGL4.82[hRluc Puro]
pRL-SV40
pBIND-GR
pGL4.24[luc2P minP]
pGL4.83[hRlucP Puro]
pRL-TK
pCBG68-Basic
pGL4.25[luc2CP minP]
pGL4.84[hRlucCP Puro]
pSELECT-zeo-Lucia
pCBG68-Control
pGL4.26[luc2 minP Hygro]
pGLuc Mini-TK 2
pSF-CMV-EMCV-FLuc
pCBG99-Basic
pGL4.27[luc2P minP Hygro]
pGLuc-Basic 2
pSF-CMV-EMCV-RLuc
pCBG99-Control
pGL4.28[luc2CP minP Hygro]
phRG-B
pSF-CMV-FLuc
pCBR-Basic
pGL4.29[luc2P CRE Hygro]
phRG-TK
pSF-CMV-FMDV-FLuc
pCBR-Control
pGL4.30[luc2P NFAT-RE Hygro]
phRL-CMV
pSF-CMV-FMDV-RLuc
pCLuc Mini-TK 2
pGL4.31[luc2P GAL4UAS Hygro]
phRL-null
pSF-CMV-PGK-FLuc
pCLuc-Basic 2
pGL4.32[luc2P NF-kB-RE Hygro]
phRL-SV40
pSF-CMV-PGK-RLuc
pCMV-CLuc 2
pGL4.33[luc2P SRE Hygro]
phRL-TK
pSF-CMV-RLuc
pCMV-Cypridina Luc
pGL4.34[luc2P SRF-RE Hygro]
phRL-TK(Int-)
pSF-CMV-RSV-FLuc AscI
pCMV-Gaussia Luc
pGL4.35[luc2P 9XGAL4UAS Hygro]
pLightSwitch_Prom
pSF-CMV-RSV-RLuc AscI
pCMV-Gaussia-Dura Luc
pGL4.36[luc2P MMTV Hygro]
pLVX-MetLuc Control
pSF-CMV-Ub-FLuc AscI
pCMV-GLuc 2
pGL4.37[luc2P ARE Hygro]
pLVX-MetLuc Reporter
pSF-CMV-Ub-RLuc AscI
pCMV-Green Renilla Luc
pGL4.38[luc2P p53 RE Hygro]
pMCS-Cypridina Luc
pSF-CMVe-FLuc
pCMV-Red Firefly Luc
pGL4.39[luc2P ATF6 RE Hygro]
pMCS-Gaussia Luc
pSF-CMVe-RLuc
pCRE-MetLuc2-Reporter
pGL4.40[luc2P MRE Hygro]
pMCS-Gaussia-Dura Luc
pSF-MinProm-FLuc
pFN26A (BIND) hRluc-neo Flexi Vector
pGL4.41[luc2P HSE Hygro]
pMCS-Green Renilla Luc
pSF-MinProm-RLuc
pGEM-luc
pGL4.42[luc2P HRE Hygro]
pMCS-Red Firefly Luc
pSF-pA-CMVe-FLuc
pGL2-Basic
pGL4.43[luc2P XRE Hygro]
pMetLuc-Mem Control
pSF-pA-CMVe-RLuc
pGL2-Control
pGL4.44[luc2P AP1 RE Hygro]
pMetLuc-Mem Reporter
pSF-pA-MinProm-FLuc
pGL2-Enhancer
pGL4.45[luc2P ISRE Hygro]
pMetLuc2-Control
pSF-pA-MinProm-RLuc
pGL2-Promoter
pGL4.47[luc2P SIE Hygro]
pMetLuc2-Reporter
pSF-pA-PromMCS-FLuc
pGL3-Basic
pGL4.48[luc2P SBE Hygro]
pmirGLO
pSF-pA-PromMCS-RLuc
pGL3-Control
pGL4.49[luc2P TCF-LEF RE Hygro]
pMirTarget
pSF-PromMCS-FLuc
pGL3-Enhancer
pGL4.50[luc2 CMV Hygro]
pNFkB-MetLuc2-Reporter
pSF-PromMCS-RLuc
pGL3-Promoter
pGL4.51[luc2 CMV Neo]
pNL1.1.CMV[Nluc CMV]
pSF-RecA-FLuc
pGL4.10[luc2]
pGL4.52[luc2P STAT5 RE Hygro]
pNL1.1[Nluc]
pSP-luc+NF
pGL4.11[luc2P]
pGL4.70[hRluc]
pNL1.2[NlucP]
pSV40-CLuc
pGL4.12[luc2CP]
pGL4.71[hRlucP]
pNL1.3.CMV[secNluc CMV]
pSV40-GLuc
pGL4.13[luc2 SV40]
pGL4.72[hRlucCP]
pNL1.3[secNluc]
pTK-CLuc
pGL4.14[luc2 Hygro]
pGL4.73[hRluc SV40]
pNL2.1[Nluc Hygro]
pTK-Cypridina Luc
pGL4.15[luc2P Hygro]
pGL4.74[hRluc TK]
pNL2.2[NlucP Hygro]
pTK-Gaussia Luc
pGL4.16[luc2CP Hygro]
pGL4.75[hRluc CMV]
pNL2.3[secNluc Hygro]
pTK-Gaussia-Dura Luc
pGL4.17[luc2 Neo]
pGL4.76[hRluc Hygro]
pNL3.1[Nluc minP]
pTK-GLuc
pGL4.18[luc2P Neo]
pGL4.77[hRlucP Hygro]
pNL3.2.NF-kB-RE[NlucP NF-kB-RE Hygro]
pTK-Green Renilla Luc
pGL4.19[luc2CP Neo]
pGL4.78[hRlucCP Hygro]
pNL3.2[NlucP minP]
pTK-Red Firefly Luc
pGL4.20[luc2 Puro]
pGL4.79[hRluc Neo]
pNL3.3[secNluc minP]
pXPG
pGL4.21[luc2P Puro]
pGL4.80[hRlucP Neo]
pRL-CMV

Plasmid Sets

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I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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