pGL3-Enhancer

SV40 enhancer-containing vector for measuring the activity of promoter sequences with a luciferase assay.

Sequence Author: Promega

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HomePlasmidsLuciferase VectorspGL3-Enhancer
Eco53kI (9) KpnI (5) Acc65I (1) BsgI (5055) RVprimer3 (5006 .. 5025) poly(A) signal NotI (4897) DraIII (4551) BsaAI (4548) BtgZI (4543) XmnI (3998) AseI (3571) NmeAIII (3547) BsaI (3460) AhdI (3399) AlwNI (2922) SacI (11) MluI (15) NheI (21) BmtI (25) TspMI - XmaI (26) SmaI - SrfI (28) MCS PaeR7I - XhoI (32) BglII (36) HindIII (53) NcoI - StyI (86) GLprimer2 (89 .. 111) KasI (120) NarI (121) SfoI (122) PluTI (124) PfoI * (195) BstBI (257) BsrGI (578) BclI * (668) XcmI (823) EcoO109I - PpuMI (1267) SgrAI (1516) XbaI (1742) FseI (1761) HpaI (1902) BsaBI * (2003) CsiI - SexAI * (2152) BamHI (2250) SalI (2256) AccI (2257) PshAI (2321) RVprimer4 (2307 .. 2326) AfeI (2382) PciI (2506) pGL3-Enhancer 5064 bp
Eco53kI  (9)
1 site		 G A G C T C C T C G A G
KpnI  (5)
1 site		 G G T A C C C C A T G G
Acc65I  (1)
1 site		 G G T A C C C C A T G G
BsgI  (5055)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
NotI  (4897)
1 site		 G C G G C C G C C G C C G G C G
DraIII  (4551)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
BsaAI  (4548)
1 site		 Y A C G T R R T G C A Y
BtgZI  (4543)
1 site		 G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4
Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
XmnI  (3998)
1 site		 G A A N N N N T T C C T T N N N N A A G
AseI  (3571)
1 site		 A T T A A T T A A T T A
NmeAIII  (3547)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaI  (3460)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3399)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2922)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
SacI  (11)
1 site		 G A G C T C C T C G A G
MluI  (15)
1 site		 A C G C G T T G C G C A
NheI  (21)
1 site		 G C T A G C C G A T C G
BmtI  (25)
1 site		 G C T A G C C G A T C G
TspMI  (26)
1 site		 C C C G G G G G G C C C
XmaI  (26)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (28)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
SrfI  (28)
1 site		 G C C C G G G C C G G G C C C G
PaeR7I  (32)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (32)
1 site		 C T C G A G G A G C T C
BglII  (36)
1 site		 A G A T C T T C T A G A
HindIII  (53)
1 site		 A A G C T T T T C G A A
NcoI  (86)
1 site		 C C A T G G G G T A C C
StyI  (86)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
KasI  (120)
1 site		 G G C G C C C C G C G G
NarI  (121)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (122)
1 site		 G G C G C C C C G C G G
PluTI  (124)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
PfoI  (195)
1 site		 T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BstBI  (257)
1 site		 T T C G A A A A G C T T
BsrGI  (578)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
BclI  (668)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
XcmI  (823)
1 site		 C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C
The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
EcoO109I  (1267)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
PpuMI  (1267)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
SgrAI  (1516)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
XbaI  (1742)
1 site		 T C T A G A A G A T C T
FseI  (1761)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
HpaI  (1902)
1 site		 G T T A A C C A A T T G
BsaBI  (2003)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
CsiI  (2152)
1 site		 A C C W G G T T G G W C C A
Sticky ends from different CsiI sites may not be compatible.
SexAI  (2152)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BamHI  (2250)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SalI  (2256)
1 site		 G T C G A C C A G C T G
AccI  (2257)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
PshAI  (2321)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AfeI  (2382)
1 site		 A G C G C T T C G C G A
PciI  (2506)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
RVprimer3
20-mer  /  50% GC
1 binding site
5006 .. 5025  =  20 annealed bases
Tm  =  54°C
GLprimer2
23-mer  /  39% GC
1 binding site
89 .. 111  =  23 annealed bases
Tm  =  57°C
RVprimer4
20-mer  /  65% GC
1 binding site
2307 .. 2326  =  20 annealed bases
Tm  =  62°C
luciferase
88 .. 1740  =  1653 bp
550 amino acids  =  60.6 kDa
Product: firefly luciferase
enhanced luc+ version of the luciferase gene
luciferase
88 .. 1740  =  1653 bp
550 amino acids  =  60.6 kDa
Product: firefly luciferase
enhanced luc+ version of the luciferase gene
AmpR
3326 .. 4186  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   3326 .. 4117  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3326 .. 4186  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   4118 .. 4186  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3326 .. 4186  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
2567 .. 3155  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
ori
2567 .. 3155  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
f1 ori
4318 .. 4773  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
4318 .. 4773  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SV40 enhancer
2020 .. 2249  =  230 bp
SV40 enhancer
2020 .. 2249  =  230 bp
SV40 poly(A) signal
1781 .. 1902  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1781 .. 1902  =  122 bp
SV40 polyadenylation signal
AmpR promoter
4187 .. 4291  =  105 bp
AmpR promoter
4187 .. 4291  =  105 bp
pause site
4966 .. 5057  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
pause site
4966 .. 5057  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
MCS
1 .. 58  =  58 bp
multiple cloning site
MCS
1 .. 58  =  58 bp
multiple cloning site
poly(A) signal
4904 .. 4952  =  49 bp
synthetic polyadenylation signal
poly(A) signal
4904 .. 4952  =  49 bp
synthetic polyadenylation signal
ORF:  88 .. 1740  =  1653 bp
ORF:  550 amino acids  =  60.6 kDa
ORF:  3456 .. 3722  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  373 .. 600  =  228 bp
ORF:  75 amino acids  =  9.1 kDa
ORF:  4851 .. 107  =  321 bp
ORF:  106 amino acids  =  12.5 kDa
ORF:  3326 .. 4186  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Individual Sequences & Maps

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pGL4.22[luc2CP Puro]
pGL4.81[hRlucCP Neo]
pRL-null
pBIND-ER(alpha)
pGL4.23[luc2 minP]
pGL4.82[hRluc Puro]
pRL-SV40
pBIND-GR
pGL4.24[luc2P minP]
pGL4.83[hRlucP Puro]
pRL-TK
pCBG68-Basic
pGL4.25[luc2CP minP]
pGL4.84[hRlucCP Puro]
pSELECT-zeo-Lucia
pCBG68-Control
pGL4.26[luc2 minP Hygro]
pGLuc Mini-TK 2
pSF-CMV-EMCV-FLuc
pCBG99-Basic
pGL4.27[luc2P minP Hygro]
pGLuc-Basic 2
pSF-CMV-EMCV-RLuc
pCBG99-Control
pGL4.28[luc2CP minP Hygro]
phRG-B
pSF-CMV-FLuc
pCBR-Basic
pGL4.29[luc2P CRE Hygro]
phRG-TK
pSF-CMV-FMDV-FLuc
pCBR-Control
pGL4.30[luc2P NFAT-RE Hygro]
phRL-CMV
pSF-CMV-FMDV-RLuc
pCLuc Mini-TK 2
pGL4.31[luc2P GAL4UAS Hygro]
phRL-null
pSF-CMV-PGK-FLuc
pCLuc-Basic 2
pGL4.32[luc2P NF-kB-RE Hygro]
phRL-SV40
pSF-CMV-PGK-RLuc
pCMV-CLuc 2
pGL4.33[luc2P SRE Hygro]
phRL-TK
pSF-CMV-RLuc
pCMV-Cypridina Luc
pGL4.34[luc2P SRF-RE Hygro]
phRL-TK(Int-)
pSF-CMV-RSV-FLuc AscI
pCMV-Gaussia Luc
pGL4.35[luc2P 9XGAL4UAS Hygro]
pLightSwitch_Prom
pSF-CMV-RSV-RLuc AscI
pCMV-Gaussia-Dura Luc
pGL4.36[luc2P MMTV Hygro]
pLVX-MetLuc Control
pSF-CMV-Ub-FLuc AscI
pCMV-GLuc 2
pGL4.37[luc2P ARE Hygro]
pLVX-MetLuc Reporter
pSF-CMV-Ub-RLuc AscI
pCMV-Green Renilla Luc
pGL4.38[luc2P p53 RE Hygro]
pMCS-Cypridina Luc
pSF-CMVe-FLuc
pCMV-Red Firefly Luc
pGL4.39[luc2P ATF6 RE Hygro]
pMCS-Gaussia Luc
pSF-CMVe-RLuc
pCRE-MetLuc2-Reporter
pGL4.40[luc2P MRE Hygro]
pMCS-Gaussia-Dura Luc
pSF-MinProm-FLuc
pFN26A (BIND) hRluc-neo Flexi Vector
pGL4.41[luc2P HSE Hygro]
pMCS-Green Renilla Luc
pSF-MinProm-RLuc
pGEM-luc
pGL4.42[luc2P HRE Hygro]
pMCS-Red Firefly Luc
pSF-pA-CMVe-FLuc
pGL2-Basic
pGL4.43[luc2P XRE Hygro]
pMetLuc-Mem Control
pSF-pA-CMVe-RLuc
pGL2-Control
pGL4.44[luc2P AP1 RE Hygro]
pMetLuc-Mem Reporter
pSF-pA-MinProm-FLuc
pGL2-Enhancer
pGL4.45[luc2P ISRE Hygro]
pMetLuc2-Control
pSF-pA-MinProm-RLuc
pGL2-Promoter
pGL4.47[luc2P SIE Hygro]
pMetLuc2-Reporter
pSF-pA-PromMCS-FLuc
pGL3-Basic
pGL4.48[luc2P SBE Hygro]
pmirGLO
pSF-pA-PromMCS-RLuc
pGL3-Control
pGL4.49[luc2P TCF-LEF RE Hygro]
pMirTarget
pSF-PromMCS-FLuc
pGL3-Enhancer
pGL4.50[luc2 CMV Hygro]
pNFkB-MetLuc2-Reporter
pSF-PromMCS-RLuc
pGL3-Promoter
pGL4.51[luc2 CMV Neo]
pNL1.1.CMV[Nluc CMV]
pSF-RecA-FLuc
pGL4.10[luc2]
pGL4.52[luc2P STAT5 RE Hygro]
pNL1.1[Nluc]
pSP-luc+NF
pGL4.11[luc2P]
pGL4.70[hRluc]
pNL1.2[NlucP]
pSV40-CLuc
pGL4.12[luc2CP]
pGL4.71[hRlucP]
pNL1.3.CMV[secNluc CMV]
pSV40-GLuc
pGL4.13[luc2 SV40]
pGL4.72[hRlucCP]
pNL1.3[secNluc]
pTK-CLuc
pGL4.14[luc2 Hygro]
pGL4.73[hRluc SV40]
pNL2.1[Nluc Hygro]
pTK-Cypridina Luc
pGL4.15[luc2P Hygro]
pGL4.74[hRluc TK]
pNL2.2[NlucP Hygro]
pTK-Gaussia Luc
pGL4.16[luc2CP Hygro]
pGL4.75[hRluc CMV]
pNL2.3[secNluc Hygro]
pTK-Gaussia-Dura Luc
pGL4.17[luc2 Neo]
pGL4.76[hRluc Hygro]
pNL3.1[Nluc minP]
pTK-GLuc
pGL4.18[luc2P Neo]
pGL4.77[hRlucP Hygro]
pNL3.2.NF-kB-RE[NlucP NF-kB-RE Hygro]
pTK-Green Renilla Luc
pGL4.19[luc2CP Neo]
pGL4.78[hRlucCP Hygro]
pNL3.2[NlucP minP]
pTK-Red Firefly Luc
pGL4.20[luc2 Puro]
pGL4.79[hRluc Neo]
pNL3.3[secNluc minP]
pXPG
pGL4.21[luc2P Puro]
pGL4.80[hRlucP Neo]
pRL-CMV

Plasmid Sets

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TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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