pGEM-luc

Plasmid carrying the firefly luciferase gene flanked by unique restriction sites.

Sequence Author: Promega

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HomePlasmidsLuciferase VectorspGEM-luc
SfiI (17) MscI (11) DraIII (4529) NaeI (4423) NgoMIV (4421) BstAPI (4244) NdeI (4243) AatII (3994) ZraI (3992) XmnI (3671) ScaI (3552) BsaI (3133) AhdI (3072) AlwNI (2595) Eco53kI (25) SacI (27) SalI (35) AccI (36) AbsI - PaeR7I - PspXI - XhoI (41) StuI (47) EcoNI (135) BseRI (200) SgrAI (321) BfuAI - BspMI (351) BspDI - ClaI (386) EcoRV (415) PacI (431) PpuMI (571) XcmI (1019) Bsu36I (1137) BstEII (1141) BsrGI (1259) BsiWI (1595) KasI (1717) NarI (1718) SfoI (1719) PluTI (1721) BamHI (1757) PspOMI (1763) ApaI (1767) NotI (1777) NsiI (1788) HindIII (1790) PciI (2179) pGEM®-luc 4931 bp
SfiI  (17)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
MscI  (11)
1 site		 T G G C C A A C C G G T
DraIII  (4529)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
NaeI  (4423)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (4421)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
BstAPI  (4244)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
NdeI  (4243)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
AatII  (3994)
1 site		 G A C G T C C T G C A G
ZraI  (3992)
1 site		 G A C G T C C T G C A G
XmnI  (3671)
1 site		 G A A N N N N T T C C T T N N N N A A G
ScaI  (3552)
1 site		 A G T A C T T C A T G A
BsaI  (3133)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3072)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2595)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
Eco53kI  (25)
1 site		 G A G C T C C T C G A G
SacI  (27)
1 site		 G A G C T C C T C G A G
SalI  (35)
1 site		 G T C G A C C A G C T G
AccI  (36)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
AbsI  (41)
1 site		 C C T C G A G G G G A G C T C C
PaeR7I  (41)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (41)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (41)
1 site		 C T C G A G G A G C T C
StuI  (47)
1 site		 A G G C C T T C C G G A
EcoNI  (135)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BseRI  (200)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
SgrAI  (321)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BfuAI  (351)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (351)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BspDI  (386)
1 site		 A T C G A T T A G C T A
ClaI  (386)
1 site		 A T C G A T T A G C T A
EcoRV  (415)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PacI  (431)
1 site		 T T A A T T A A A A T T A A T T
PpuMI  (571)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
XcmI  (1019)
1 site		 C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C
The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
Bsu36I  (1137)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
BstEII  (1141)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BsrGI  (1259)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
BsiWI  (1595)
1 site		 C G T A C G G C A T G C
BsiWI is typically used at 55°C, but is 50% active at 37°C.
KasI  (1717)
1 site		 G G C G C C C C G C G G
NarI  (1718)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (1719)
1 site		 G G C G C C C C G C G G
PluTI  (1721)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BamHI  (1757)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
PspOMI  (1763)
1 site		 G G G C C C C C C G G G
ApaI  (1767)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
NotI  (1777)
1 site		 G C G G C C G C C G C C G G C G
NsiI  (1788)
1 site		 A T G C A T T A C G T A
HindIII  (1790)
1 site		 A A G C T T T T C G A A
PciI  (2179)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
luciferase
102 .. 1754  =  1653 bp
550 amino acids  =  60.7 kDa
Product: firefly luciferase
luciferase
102 .. 1754  =  1653 bp
550 amino acids  =  60.7 kDa
Product: firefly luciferase
AmpR
2999 .. 3859  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2999 .. 3790  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2999 .. 3859  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3791 .. 3859  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2999 .. 3859  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
2240 .. 2828  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
ori
2240 .. 2828  =  589 bp
high-copy-number colE1/pMB1/pBR322/pUC origin of replication
f1 ori
4296 .. 4751  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
4296 .. 4751  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
AmpR promoter
3860 .. 3964  =  105 bp
AmpR promoter
3860 .. 3964  =  105 bp
MCS
9 .. 50  =  42 bp
multiple cloning site
MCS
9 .. 50  =  42 bp
multiple cloning site
MCS
1757 .. 1795  =  39 bp
multiple cloning site
MCS
1757 .. 1795  =  39 bp
multiple cloning site
lac promoter
1886 .. 1916  =  31 bp
3 segments
   Segment 3:  -10  
   1886 .. 1892  =  7 bp
promoter for the E. coli lac operon
lac promoter
1886 .. 1916  =  31 bp
3 segments
   Segment 2:  
   1893 .. 1910  =  18 bp
promoter for the E. coli lac operon
lac promoter
1886 .. 1916  =  31 bp
3 segments
   Segment 1:  -35  
   1911 .. 1916  =  6 bp
promoter for the E. coli lac operon
lac promoter
1886 .. 1916  =  31 bp
3 segments
promoter for the E. coli lac operon
SP6 promoter
1802 .. 1820  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
SP6 promoter
1802 .. 1820  =  19 bp
promoter for bacteriophage SP6 RNA polymerase
T7 promoter
4915 .. 2  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
4915 .. 2  =  19 bp
promoter for bacteriophage T7 RNA polymerase
lac operator
1862 .. 1878  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
1862 .. 1878  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
ORF:  4268 .. 4513  =  246 bp
ORF:  81 amino acids  =  8.7 kDa
ORF:  3129 .. 3395  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  102 .. 1754  =  1653 bp
ORF:  550 amino acids  =  60.7 kDa
ORF:  2999 .. 3859  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Individual Sequences & Maps

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pGL4.22[luc2CP Puro]
pGL4.81[hRlucCP Neo]
pRL-null
pBIND-ER(alpha)
pGL4.23[luc2 minP]
pGL4.82[hRluc Puro]
pRL-SV40
pBIND-GR
pGL4.24[luc2P minP]
pGL4.83[hRlucP Puro]
pRL-TK
pCBG68-Basic
pGL4.25[luc2CP minP]
pGL4.84[hRlucCP Puro]
pSELECT-zeo-Lucia
pCBG68-Control
pGL4.26[luc2 minP Hygro]
pGLuc Mini-TK 2
pSF-CMV-EMCV-FLuc
pCBG99-Basic
pGL4.27[luc2P minP Hygro]
pGLuc-Basic 2
pSF-CMV-EMCV-RLuc
pCBG99-Control
pGL4.28[luc2CP minP Hygro]
phRG-B
pSF-CMV-FLuc
pCBR-Basic
pGL4.29[luc2P CRE Hygro]
phRG-TK
pSF-CMV-FMDV-FLuc
pCBR-Control
pGL4.30[luc2P NFAT-RE Hygro]
phRL-CMV
pSF-CMV-FMDV-RLuc
pCLuc Mini-TK 2
pGL4.31[luc2P GAL4UAS Hygro]
phRL-null
pSF-CMV-PGK-FLuc
pCLuc-Basic 2
pGL4.32[luc2P NF-kB-RE Hygro]
phRL-SV40
pSF-CMV-PGK-RLuc
pCMV-CLuc 2
pGL4.33[luc2P SRE Hygro]
phRL-TK
pSF-CMV-RLuc
pCMV-Cypridina Luc
pGL4.34[luc2P SRF-RE Hygro]
phRL-TK(Int-)
pSF-CMV-RSV-FLuc AscI
pCMV-Gaussia Luc
pGL4.35[luc2P 9XGAL4UAS Hygro]
pLightSwitch_Prom
pSF-CMV-RSV-RLuc AscI
pCMV-Gaussia-Dura Luc
pGL4.36[luc2P MMTV Hygro]
pLVX-MetLuc Control
pSF-CMV-Ub-FLuc AscI
pCMV-GLuc 2
pGL4.37[luc2P ARE Hygro]
pLVX-MetLuc Reporter
pSF-CMV-Ub-RLuc AscI
pCMV-Green Renilla Luc
pGL4.38[luc2P p53 RE Hygro]
pMCS-Cypridina Luc
pSF-CMVe-FLuc
pCMV-Red Firefly Luc
pGL4.39[luc2P ATF6 RE Hygro]
pMCS-Gaussia Luc
pSF-CMVe-RLuc
pCRE-MetLuc2-Reporter
pGL4.40[luc2P MRE Hygro]
pMCS-Gaussia-Dura Luc
pSF-MinProm-FLuc
pFN26A (BIND) hRluc-neo Flexi Vector
pGL4.41[luc2P HSE Hygro]
pMCS-Green Renilla Luc
pSF-MinProm-RLuc
pGEM-luc
pGL4.42[luc2P HRE Hygro]
pMCS-Red Firefly Luc
pSF-pA-CMVe-FLuc
pGL2-Basic
pGL4.43[luc2P XRE Hygro]
pMetLuc-Mem Control
pSF-pA-CMVe-RLuc
pGL2-Control
pGL4.44[luc2P AP1 RE Hygro]
pMetLuc-Mem Reporter
pSF-pA-MinProm-FLuc
pGL2-Enhancer
pGL4.45[luc2P ISRE Hygro]
pMetLuc2-Control
pSF-pA-MinProm-RLuc
pGL2-Promoter
pGL4.47[luc2P SIE Hygro]
pMetLuc2-Reporter
pSF-pA-PromMCS-FLuc
pGL3-Basic
pGL4.48[luc2P SBE Hygro]
pmirGLO
pSF-pA-PromMCS-RLuc
pGL3-Control
pGL4.49[luc2P TCF-LEF RE Hygro]
pMirTarget
pSF-PromMCS-FLuc
pGL3-Enhancer
pGL4.50[luc2 CMV Hygro]
pNFkB-MetLuc2-Reporter
pSF-PromMCS-RLuc
pGL3-Promoter
pGL4.51[luc2 CMV Neo]
pNL1.1.CMV[Nluc CMV]
pSF-RecA-FLuc
pGL4.10[luc2]
pGL4.52[luc2P STAT5 RE Hygro]
pNL1.1[Nluc]
pSP-luc+NF
pGL4.11[luc2P]
pGL4.70[hRluc]
pNL1.2[NlucP]
pSV40-CLuc
pGL4.12[luc2CP]
pGL4.71[hRlucP]
pNL1.3.CMV[secNluc CMV]
pSV40-GLuc
pGL4.13[luc2 SV40]
pGL4.72[hRlucCP]
pNL1.3[secNluc]
pTK-CLuc
pGL4.14[luc2 Hygro]
pGL4.73[hRluc SV40]
pNL2.1[Nluc Hygro]
pTK-Cypridina Luc
pGL4.15[luc2P Hygro]
pGL4.74[hRluc TK]
pNL2.2[NlucP Hygro]
pTK-Gaussia Luc
pGL4.16[luc2CP Hygro]
pGL4.75[hRluc CMV]
pNL2.3[secNluc Hygro]
pTK-Gaussia-Dura Luc
pGL4.17[luc2 Neo]
pGL4.76[hRluc Hygro]
pNL3.1[Nluc minP]
pTK-GLuc
pGL4.18[luc2P Neo]
pGL4.77[hRlucP Hygro]
pNL3.2.NF-kB-RE[NlucP NF-kB-RE Hygro]
pTK-Green Renilla Luc
pGL4.19[luc2CP Neo]
pGL4.78[hRlucCP Hygro]
pNL3.2[NlucP minP]
pTK-Red Firefly Luc
pGL4.20[luc2 Puro]
pGL4.79[hRluc Neo]
pNL3.3[secNluc minP]
pXPG
pGL4.21[luc2P Puro]
pGL4.80[hRlucP Neo]
pRL-CMV

Plasmid Sets

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I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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