pSF-pA-MinProm-FLuc

Vector encoding a minimal promoter and firefly luciferase, for measuring the activity of transcriptional regulatory elements.

Sequence Author: Oxford Genetics

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HomePlasmidsLuciferase VectorspSF-pA-MinProm-FLuc
AsiSI - PvuI (5) PmeI (5540) RsrII (5233) BsrDI (4950) PflFI - Tth111I (4835) PmeI (4492) PpuMI - SanDI (4426) AscI - BssHII (4335) FseI (4189) SwaI (4083) AlwNI (3610) ApaLI (3508) SwaI (3190) PacI (3064) BstEII (3038) 3' β-globin insulator SbfI (2932) BglI (2755) 5' β-globin insulator BglII (232) NruI (318) PshAI (324) poly(A) signal AarI (462) SalI (493) AccI (494) SpeI (501) TspMI - XmaI (509) SmaI (511) MCS PspOMI (517) ApaI (521) BstBI (526) MluI (551) BglII (612) EagI - NotI (654) HindIII (665) Eco53kI (675) SacI (677) EcoRI (681) Acc65I (693) KpnI (697) NcoI (701) BbsI (761) PvuII (777) BsrGI (1193) AleI (1201) FspI (1525) KasI (1650) NarI (1651) SfoI (1652) PluTI (1654) BlpI (1657) AgeI (1834) DraIII (1849) BpmI (2083) XbaI (2351) BseRI - BsgI (2354) stop codons BspDI - ClaI (2391) BamHI (2400) StuI (2410) NheI (2416) BmtI (2420) stop codons BtsI (2512) PsiI (2566) MfeI (2595) pSF-pA-MinProm-FLuc 5659 bp
AsiSI  (5)
1 site		 G C G A T C G C C G C T A G C G
PvuI  (5)
1 site		 C G A T C G G C T A G C
PmeI  (5540)
2 sites		 G T T T A A A C C A A A T T T G
RsrII  (5233)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (4950)
1 site		 G C A A T G N N C G T T A C
Sticky ends from different BsrDI sites may not be compatible.
PflFI  (4835)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4835)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PmeI  (4492)
2 sites		 G T T T A A A C C A A A T T T G
PpuMI  (4426)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
SanDI  (4426)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different SanDI sites may not be compatible.
AscI  (4335)
1 site		 G G C G C G C C C C G C G C G G
BssHII  (4335)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
FseI  (4189)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
SwaI  (4083)
2 sites		 A T T T A A A T T A A A T T T A
SwaI is typically used at 25°C, but is 50% active at 37°C.
AlwNI  (3610)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
ApaLI  (3508)
1 site		 G T G C A C C A C G T G
SwaI  (3190)
2 sites		 A T T T A A A T T A A A T T T A
SwaI is typically used at 25°C, but is 50% active at 37°C.
PacI  (3064)
1 site		 T T A A T T A A A A T T A A T T
BstEII  (3038)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
SbfI  (2932)
1 site		 C C T G C A G G G G A C G T C C
BglI  (2755)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
BglII  (232)
2 sites		 A G A T C T T C T A G A
NruI  (318)
1 site		 T C G C G A A G C G C T
PshAI  (324)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AarI  (462)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Cleavage may be enhanced when more than one copy of the AarI recognition sequence is present.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
SalI  (493)
1 site		 G T C G A C C A G C T G
AccI  (494)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SpeI  (501)
1 site		 A C T A G T T G A T C A
TspMI  (509)
1 site		 C C C G G G G G G C C C
XmaI  (509)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (511)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
PspOMI  (517)
1 site		 G G G C C C C C C G G G
ApaI  (521)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
BstBI  (526)
1 site		 T T C G A A A A G C T T
MluI  (551)
1 site		 A C G C G T T G C G C A
BglII  (612)
2 sites		 A G A T C T T C T A G A
EagI  (654)
1 site		 C G G C C G G C C G G C
NotI  (654)
1 site		 G C G G C C G C C G C C G G C G
HindIII  (665)
1 site		 A A G C T T T T C G A A
Eco53kI  (675)
1 site		 G A G C T C C T C G A G
SacI  (677)
1 site		 G A G C T C C T C G A G
EcoRI  (681)
1 site		 G A A T T C C T T A A G
Acc65I  (693)
1 site		 G G T A C C C C A T G G
KpnI  (697)
1 site		 G G T A C C C C A T G G
NcoI  (701)
1 site		 C C A T G G G G T A C C
BbsI  (761)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
PvuII  (777)
1 site		 C A G C T G G T C G A C
BsrGI  (1193)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
AleI  (1201)
1 site		 C A C N N N N G T G G T G N N N N C A C
FspI  (1525)
1 site		 T G C G C A A C G C G T
KasI  (1650)
1 site		 G G C G C C C C G C G G
NarI  (1651)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (1652)
1 site		 G G C G C C C C G C G G
PluTI  (1654)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BlpI  (1657)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
AgeI  (1834)
1 site		 A C C G G T T G G C C A
DraIII  (1849)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
BpmI  (2083)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
XbaI  (2351)
1 site		 T C T A G A A G A T C T
BseRI  (2354)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BsgI  (2354)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BspDI  (2391)
1 site		 A T C G A T T A G C T A
ClaI  (2391)
1 site		 A T C G A T T A G C T A
BamHI  (2400)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
StuI  (2410)
1 site		 A G G C C T T C C G G A
NheI  (2416)
1 site		 G C T A G C C G A T C G
BmtI  (2420)
1 site		 G C T A G C C G A T C G
BtsI  (2512)
1 site		 G C A G T G N N C G T C A C
PsiI  (2566)
1 site		 T T A T A A A A T A T T
MfeI  (2595)
1 site		 C A A T T G G T T A A C
luciferase
703 .. 2355  =  1653 bp
550 amino acids  =  60.6 kDa
Product: firefly luciferase
synthetic luc2 version of the luciferase gene
luciferase
703 .. 2355  =  1653 bp
550 amino acids  =  60.6 kDa
Product: firefly luciferase
synthetic luc2 version of the luciferase gene
NeoR/KanR
4589 .. 5383  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
4589 .. 5383  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori
3255 .. 3843  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3255 .. 3843  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
rrnG terminator
2777 .. 2913  =  137 bp
transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)
rrnG terminator
2777 .. 2913  =  137 bp
transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)
rrnG terminator
5396 .. 5532  =  137 bp
transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)
rrnG terminator
5396 .. 5532  =  137 bp
transcription terminator from the E. coli ribosomal RNA rrnG operon (Albrechtsen et al., 1991)
SV40 poly(A) signal
2465 .. 2586  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
2465 .. 2586  =  122 bp
SV40 polyadenylation signal
pause site
401 .. 492  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
pause site
401 .. 492  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
5' β-globin insulator
18 .. 89  =  72 bp
insulator upstream of the human β-globin locus (Farrell et al., 2002)
5' β-globin insulator
18 .. 89  =  72 bp
insulator upstream of the human β-globin locus (Farrell et al., 2002)
3' β-globin insulator
2958 .. 3029  =  72 bp
insulator downstream of the human β-globin locus (Farrell et al., 2002)
3' β-globin insulator
2958 .. 3029  =  72 bp
insulator downstream of the human β-globin locus (Farrell et al., 2002)
Mini-TK promoter
535 .. 597  =  63 bp
minimal herpes simplex virus (HSV) thymidine kinase promoter
Mini-TK promoter
535 .. 597  =  63 bp
minimal herpes simplex virus (HSV) thymidine kinase promoter
poly(A) signal
339 .. 387  =  49 bp
synthetic polyadenylation signal
poly(A) signal
339 .. 387  =  49 bp
synthetic polyadenylation signal
T7 terminator
2701 .. 2747  =  47 bp
transcription terminator for bacteriophage T7 RNA polymerase
T7 terminator
2701 .. 2747  =  47 bp
transcription terminator for bacteriophage T7 RNA polymerase
MCS
493 .. 530  =  38 bp
multiple cloning site
MCS
493 .. 530  =  38 bp
multiple cloning site
stop codons
2375 .. 2385  =  11 bp
stop codons in all three reading frames
stop codons
2375 .. 2385  =  11 bp
stop codons in all three reading frames
stop codons
2423 .. 2433  =  11 bp
stop codons in all three reading frames
stop codons
2423 .. 2433  =  11 bp
stop codons in all three reading frames
RBS
689 .. 694  =  6 bp
Shine-Dalgarno ribosome binding site
RBS
689 .. 694  =  6 bp
Shine-Dalgarno ribosome binding site
RBS
4576 .. 4581  =  6 bp
Shine-Dalgarno ribosome binding site
RBS
4576 .. 4581  =  6 bp
Shine-Dalgarno ribosome binding site
Kozak sequence
699 .. 705  =  7 bp
Kozak sequence
699 .. 705  =  7 bp
ORF:  703 .. 2355  =  1653 bp
ORF:  550 amino acids  =  60.6 kDa
ORF:  4589 .. 5383  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  2802 .. 3233  =  432 bp
ORF:  143 amino acids  =  16.3 kDa
ORF:  4761 .. 5147  =  387 bp
ORF:  128 amino acids  =  14.7 kDa
ORF:  5421 .. 196  =  435 bp
ORF:  144 amino acids  =  16.1 kDa
ORF:  262 .. 486  =  225 bp
ORF:  74 amino acids  =  9.1 kDa
ORF:  685 .. 930  =  246 bp
ORF:  81 amino acids  =  8.8 kDa
ORF:  1699 .. 1959  =  261 bp
ORF:  86 amino acids  =  8.9 kDa
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Individual Sequences & Maps

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pGL4.83[hRlucP Puro]
pRL-TK
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pGL4.25[luc2CP minP]
pGL4.84[hRlucCP Puro]
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pCBG99-Basic
pGL4.27[luc2P minP Hygro]
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pCBG99-Control
pGL4.28[luc2CP minP Hygro]
phRG-B
pSF-CMV-FLuc
pCBR-Basic
pGL4.29[luc2P CRE Hygro]
phRG-TK
pSF-CMV-FMDV-FLuc
pCBR-Control
pGL4.30[luc2P NFAT-RE Hygro]
phRL-CMV
pSF-CMV-FMDV-RLuc
pCLuc Mini-TK 2
pGL4.31[luc2P GAL4UAS Hygro]
phRL-null
pSF-CMV-PGK-FLuc
pCLuc-Basic 2
pGL4.32[luc2P NF-kB-RE Hygro]
phRL-SV40
pSF-CMV-PGK-RLuc
pCMV-CLuc 2
pGL4.33[luc2P SRE Hygro]
phRL-TK
pSF-CMV-RLuc
pCMV-Cypridina Luc
pGL4.34[luc2P SRF-RE Hygro]
phRL-TK(Int-)
pSF-CMV-RSV-FLuc AscI
pCMV-Gaussia Luc
pGL4.35[luc2P 9XGAL4UAS Hygro]
pLightSwitch_Prom
pSF-CMV-RSV-RLuc AscI
pCMV-Gaussia-Dura Luc
pGL4.36[luc2P MMTV Hygro]
pLVX-MetLuc Control
pSF-CMV-Ub-FLuc AscI
pCMV-GLuc 2
pGL4.37[luc2P ARE Hygro]
pLVX-MetLuc Reporter
pSF-CMV-Ub-RLuc AscI
pCMV-Green Renilla Luc
pGL4.38[luc2P p53 RE Hygro]
pMCS-Cypridina Luc
pSF-CMVe-FLuc
pCMV-Red Firefly Luc
pGL4.39[luc2P ATF6 RE Hygro]
pMCS-Gaussia Luc
pSF-CMVe-RLuc
pCRE-MetLuc2-Reporter
pGL4.40[luc2P MRE Hygro]
pMCS-Gaussia-Dura Luc
pSF-MinProm-FLuc
pFN26A (BIND) hRluc-neo Flexi Vector
pGL4.41[luc2P HSE Hygro]
pMCS-Green Renilla Luc
pSF-MinProm-RLuc
pGEM-luc
pGL4.42[luc2P HRE Hygro]
pMCS-Red Firefly Luc
pSF-pA-CMVe-FLuc
pGL2-Basic
pGL4.43[luc2P XRE Hygro]
pMetLuc-Mem Control
pSF-pA-CMVe-RLuc
pGL2-Control
pGL4.44[luc2P AP1 RE Hygro]
pMetLuc-Mem Reporter
pSF-pA-MinProm-FLuc
pGL2-Enhancer
pGL4.45[luc2P ISRE Hygro]
pMetLuc2-Control
pSF-pA-MinProm-RLuc
pGL2-Promoter
pGL4.47[luc2P SIE Hygro]
pMetLuc2-Reporter
pSF-pA-PromMCS-FLuc
pGL3-Basic
pGL4.48[luc2P SBE Hygro]
pmirGLO
pSF-pA-PromMCS-RLuc
pGL3-Control
pGL4.49[luc2P TCF-LEF RE Hygro]
pMirTarget
pSF-PromMCS-FLuc
pGL3-Enhancer
pGL4.50[luc2 CMV Hygro]
pNFkB-MetLuc2-Reporter
pSF-PromMCS-RLuc
pGL3-Promoter
pGL4.51[luc2 CMV Neo]
pNL1.1.CMV[Nluc CMV]
pSF-RecA-FLuc
pGL4.10[luc2]
pGL4.52[luc2P STAT5 RE Hygro]
pNL1.1[Nluc]
pSP-luc+NF
pGL4.11[luc2P]
pGL4.70[hRluc]
pNL1.2[NlucP]
pSV40-CLuc
pGL4.12[luc2CP]
pGL4.71[hRlucP]
pNL1.3.CMV[secNluc CMV]
pSV40-GLuc
pGL4.13[luc2 SV40]
pGL4.72[hRlucCP]
pNL1.3[secNluc]
pTK-CLuc
pGL4.14[luc2 Hygro]
pGL4.73[hRluc SV40]
pNL2.1[Nluc Hygro]
pTK-Cypridina Luc
pGL4.15[luc2P Hygro]
pGL4.74[hRluc TK]
pNL2.2[NlucP Hygro]
pTK-Gaussia Luc
pGL4.16[luc2CP Hygro]
pGL4.75[hRluc CMV]
pNL2.3[secNluc Hygro]
pTK-Gaussia-Dura Luc
pGL4.17[luc2 Neo]
pGL4.76[hRluc Hygro]
pNL3.1[Nluc minP]
pTK-GLuc
pGL4.18[luc2P Neo]
pGL4.77[hRlucP Hygro]
pNL3.2.NF-kB-RE[NlucP NF-kB-RE Hygro]
pTK-Green Renilla Luc
pGL4.19[luc2CP Neo]
pGL4.78[hRlucCP Hygro]
pNL3.2[NlucP minP]
pTK-Red Firefly Luc
pGL4.20[luc2 Puro]
pGL4.79[hRluc Neo]
pNL3.3[secNluc minP]
pXPG
pGL4.21[luc2P Puro]
pGL4.80[hRlucP Neo]
pRL-CMV

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
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Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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