pTK-Cypridina Luc

Control vector for constitutive low-level expression of secreted Cypridina luciferase under control of the HSV TK promoter.

Sequence Author: Thermo Fisher (Pierce)

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HomePlasmidsLuciferase VectorspTK-Cypridina Luc
SpeI (1) PaqCI (6700) DrdI (5720) MauBI (5124) NruI (5051) PvuI (4497) FspI (4349) AfeI (4177) SalI (4017) DraIII (3722) BsmBI - Esp3I (3543) BstEII (3285) PaeR7I - XhoI (7) HindIII (20) BspQI - SapI (155) BsaI (404) BstBI (671) MluI (731) BamHI (778) Bpu10I (952) BstXI (1143) BspDI - ClaI (1214) BsrDI (1271) NheI (1678) BmtI (1682) AleI (1738) BlpI (1744) Bsu36I (2051) KflI (2106) NotI (2453) BseRI (3018) PflFI - Tth111I (3193) BsiWI (3207) RsrII (3267) pTK-Cypridina Luc 6730 bp
SpeI  (1)
1 site		 A C T A G T T G A T C A
PaqCI  (6700)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
DrdI  (5720)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
MauBI  (5124)
1 site		 C G C G C G C G G C G C G C G C
NruI  (5051)
1 site		 T C G C G A A G C G C T
PvuI  (4497)
1 site		 C G A T C G G C T A G C
FspI  (4349)
1 site		 T G C G C A A C G C G T
AfeI  (4177)
1 site		 A G C G C T T C G C G A
SalI  (4017)
1 site		 G T C G A C C A G C T G
DraIII  (3722)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
BsmBI  (3543)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (3543)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
BstEII  (3285)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PaeR7I  (7)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (7)
1 site		 C T C G A G G A G C T C
HindIII  (20)
1 site		 A A G C T T T T C G A A
BspQI  (155)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (155)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BsaI  (404)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BstBI  (671)
1 site		 T T C G A A A A G C T T
MluI  (731)
1 site		 A C G C G T T G C G C A
BamHI  (778)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Bpu10I  (952)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BstXI  (1143)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
BspDI  (1214)
1 site		 A T C G A T T A G C T A
ClaI  (1214)
1 site		 A T C G A T T A G C T A
BsrDI  (1271)
1 site		 G C A A T G N N C G T T A C
Sticky ends from different BsrDI sites may not be compatible.
NheI  (1678)
1 site		 G C T A G C C G A T C G
BmtI  (1682)
1 site		 G C T A G C C G A T C G
AleI  (1738)
1 site		 C A C N N N N G T G G T G N N N N C A C
BlpI  (1744)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
Bsu36I  (2051)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
KflI  (2106)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different KflI sites may not be compatible.
NotI  (2453)
1 site		 G C G G C C G C C G C C G G C G
BseRI  (3018)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
PflFI  (3193)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3193)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsiWI  (3207)
1 site		 C G T A C G G C A T G C
BsiWI is typically used at 55°C, but is 50% active at 37°C.
RsrII  (3267)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
hCluc
790 .. 2451  =  1662 bp
553 amino acids  =  61.5 kDa
Product: secreted Cypridina luciferase
human codon-optimized
hCluc
790 .. 2451  =  1662 bp
553 amino acids  =  61.5 kDa
Product: secreted Cypridina luciferase
human codon-optimized
AmpR
4054 .. 4914  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4054 .. 4845  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4054 .. 4914  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   4846 .. 4914  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4054 .. 4914  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
HSV TK promoter
26 .. 777  =  752 bp
herpes simplex virus thymidine kinase promoter
HSV TK promoter
26 .. 777  =  752 bp
herpes simplex virus thymidine kinase promoter
PuroR
3151 .. 3750  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
3151 .. 3750  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
ori
5178 .. 5766  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
5178 .. 5766  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
SV40 promoter
2708 .. 3037  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
2708 .. 3037  =  330 bp
SV40 enhancer and early promoter
SV40 poly(A) signal
3880 .. 4001  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
3880 .. 4001  =  122 bp
SV40 polyadenylation signal
bGH poly(A) signal
2474 .. 2585  =  112 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
2474 .. 2585  =  112 bp
bovine growth hormone polyadenylation signal
pause site
6639 .. 6730  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
pause site
6639 .. 6730  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
EM7 promoter
3085 .. 3132  =  48 bp
synthetic bacterial promoter
EM7 promoter
3085 .. 3132  =  48 bp
synthetic bacterial promoter
lac operator
6508 .. 6524  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
6508 .. 6524  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
SV40 ori
2888 .. 3023  =  136 bp
SV40 origin of replication
SV40 ori
2888 .. 3023  =  136 bp
SV40 origin of replication
ORF:  790 .. 2451  =  1662 bp
ORF:  553 amino acids  =  61.5 kDa
ORF:  3151 .. 3750  =  600 bp
ORF:  199 amino acids  =  21.5 kDa
ORF:  350 .. 793  =  444 bp
ORF:  147 amino acids  =  16.5 kDa
ORF:  4184 .. 4450  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  912 .. 1529  =  618 bp
ORF:  205 amino acids  =  24.3 kDa
ORF:  1710 .. 2054  =  345 bp
ORF:  114 amino acids  =  13.1 kDa
ORF:  3311 .. 3637  =  327 bp
ORF:  108 amino acids  =  12.0 kDa
ORF:  112 .. 558  =  447 bp
ORF:  148 amino acids  =  16.0 kDa
ORF:  4054 .. 4914  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
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Individual Sequences & Maps

pBI-CMV5
pGL4.22[luc2CP Puro]
pGL4.81[hRlucCP Neo]
pRL-null
pBIND-ER(alpha)
pGL4.23[luc2 minP]
pGL4.82[hRluc Puro]
pRL-SV40
pBIND-GR
pGL4.24[luc2P minP]
pGL4.83[hRlucP Puro]
pRL-TK
pCBG68-Basic
pGL4.25[luc2CP minP]
pGL4.84[hRlucCP Puro]
pSELECT-zeo-Lucia
pCBG68-Control
pGL4.26[luc2 minP Hygro]
pGLuc Mini-TK 2
pSF-CMV-EMCV-FLuc
pCBG99-Basic
pGL4.27[luc2P minP Hygro]
pGLuc-Basic 2
pSF-CMV-EMCV-RLuc
pCBG99-Control
pGL4.28[luc2CP minP Hygro]
phRG-B
pSF-CMV-FLuc
pCBR-Basic
pGL4.29[luc2P CRE Hygro]
phRG-TK
pSF-CMV-FMDV-FLuc
pCBR-Control
pGL4.30[luc2P NFAT-RE Hygro]
phRL-CMV
pSF-CMV-FMDV-RLuc
pCLuc Mini-TK 2
pGL4.31[luc2P GAL4UAS Hygro]
phRL-null
pSF-CMV-PGK-FLuc
pCLuc-Basic 2
pGL4.32[luc2P NF-kB-RE Hygro]
phRL-SV40
pSF-CMV-PGK-RLuc
pCMV-CLuc 2
pGL4.33[luc2P SRE Hygro]
phRL-TK
pSF-CMV-RLuc
pCMV-Cypridina Luc
pGL4.34[luc2P SRF-RE Hygro]
phRL-TK(Int-)
pSF-CMV-RSV-FLuc AscI
pCMV-Gaussia Luc
pGL4.35[luc2P 9XGAL4UAS Hygro]
pLightSwitch_Prom
pSF-CMV-RSV-RLuc AscI
pCMV-Gaussia-Dura Luc
pGL4.36[luc2P MMTV Hygro]
pLVX-MetLuc Control
pSF-CMV-Ub-FLuc AscI
pCMV-GLuc 2
pGL4.37[luc2P ARE Hygro]
pLVX-MetLuc Reporter
pSF-CMV-Ub-RLuc AscI
pCMV-Green Renilla Luc
pGL4.38[luc2P p53 RE Hygro]
pMCS-Cypridina Luc
pSF-CMVe-FLuc
pCMV-Red Firefly Luc
pGL4.39[luc2P ATF6 RE Hygro]
pMCS-Gaussia Luc
pSF-CMVe-RLuc
pCRE-MetLuc2-Reporter
pGL4.40[luc2P MRE Hygro]
pMCS-Gaussia-Dura Luc
pSF-MinProm-FLuc
pFN26A (BIND) hRluc-neo Flexi Vector
pGL4.41[luc2P HSE Hygro]
pMCS-Green Renilla Luc
pSF-MinProm-RLuc
pGEM-luc
pGL4.42[luc2P HRE Hygro]
pMCS-Red Firefly Luc
pSF-pA-CMVe-FLuc
pGL2-Basic
pGL4.43[luc2P XRE Hygro]
pMetLuc-Mem Control
pSF-pA-CMVe-RLuc
pGL2-Control
pGL4.44[luc2P AP1 RE Hygro]
pMetLuc-Mem Reporter
pSF-pA-MinProm-FLuc
pGL2-Enhancer
pGL4.45[luc2P ISRE Hygro]
pMetLuc2-Control
pSF-pA-MinProm-RLuc
pGL2-Promoter
pGL4.47[luc2P SIE Hygro]
pMetLuc2-Reporter
pSF-pA-PromMCS-FLuc
pGL3-Basic
pGL4.48[luc2P SBE Hygro]
pmirGLO
pSF-pA-PromMCS-RLuc
pGL3-Control
pGL4.49[luc2P TCF-LEF RE Hygro]
pMirTarget
pSF-PromMCS-FLuc
pGL3-Enhancer
pGL4.50[luc2 CMV Hygro]
pNFkB-MetLuc2-Reporter
pSF-PromMCS-RLuc
pGL3-Promoter
pGL4.51[luc2 CMV Neo]
pNL1.1.CMV[Nluc CMV]
pSF-RecA-FLuc
pGL4.10[luc2]
pGL4.52[luc2P STAT5 RE Hygro]
pNL1.1[Nluc]
pSP-luc+NF
pGL4.11[luc2P]
pGL4.70[hRluc]
pNL1.2[NlucP]
pSV40-CLuc
pGL4.12[luc2CP]
pGL4.71[hRlucP]
pNL1.3.CMV[secNluc CMV]
pSV40-GLuc
pGL4.13[luc2 SV40]
pGL4.72[hRlucCP]
pNL1.3[secNluc]
pTK-CLuc
pGL4.14[luc2 Hygro]
pGL4.73[hRluc SV40]
pNL2.1[Nluc Hygro]
pTK-Cypridina Luc
pGL4.15[luc2P Hygro]
pGL4.74[hRluc TK]
pNL2.2[NlucP Hygro]
pTK-Gaussia Luc
pGL4.16[luc2CP Hygro]
pGL4.75[hRluc CMV]
pNL2.3[secNluc Hygro]
pTK-Gaussia-Dura Luc
pGL4.17[luc2 Neo]
pGL4.76[hRluc Hygro]
pNL3.1[Nluc minP]
pTK-GLuc
pGL4.18[luc2P Neo]
pGL4.77[hRlucP Hygro]
pNL3.2.NF-kB-RE[NlucP NF-kB-RE Hygro]
pTK-Green Renilla Luc
pGL4.19[luc2CP Neo]
pGL4.78[hRlucCP Hygro]
pNL3.2[NlucP minP]
pTK-Red Firefly Luc
pGL4.20[luc2 Puro]
pGL4.79[hRluc Neo]
pNL3.3[secNluc minP]
pXPG
pGL4.21[luc2P Puro]
pGL4.80[hRlucP Neo]
pRL-CMV

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