pLightSwitch_Prom

Vector for measuring promoter activity with an enhanced and destabilized Renilla luciferase (RenSP).

Sequence Author: SwitchGear Genomics

Try SnapGene for Free
|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Luciferase Vectors | More Plasmid Sets
No matches
HomePlasmidsLuciferase VectorspLightSwitch_Prom
SfiI (8) PaqCI (3626) Forward Sequencing Primer (3576 .. 3598) poly(A) signal BsmBI - Esp3I (3453) SpeI (3440) PvuI (2981) AseI (2783) NmeAIII (2759) AhdI (2611) BstEII (2536) BstXI - PstI (2533) AleI (2531) NotI (2509) BspHI (2409) AlwNI (2105) BciVI (1892) DrdI (1797) Acc65I (14) KpnI (18) Eco53kI (23) SacI (25) MluI (29) NheI (35) MCS BmtI (39) TspMI - XmaI (40) SmaI - SrfI (42) PaeR7I - XhoI (46) BglII (50) HindIII (67) NcoI (110) Reverse Sequencing Primer (184 .. 205) DraIII (300) SphI (538) EcoRV (585) PfoI * (635) BbvCI (760) XcmI (761) PvuII (764) BssHII (843) EcoO109I (957) EcoRI (1044) XbaI (1171) FseI (1190) SV40 poly(A) signal PsiI (1311) HpaI (1331) MfeI (1340) BamHI (1433) SalI (1439) AccI (1440) PshAI (1504) PciI (1689) pLightSwitch_ Prom 3656 bp
SfiI  (8)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
PaqCI  (3626)
1 site		 C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
BsmBI  (3453)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (3453)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
SpeI  (3440)
1 site		 A C T A G T T G A T C A
PvuI  (2981)
1 site		 C G A T C G G C T A G C
AseI  (2783)
1 site		 A T T A A T T A A T T A
NmeAIII  (2759)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AhdI  (2611)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BstEII  (2536)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BstXI  (2533)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
PstI  (2533)
1 site		 C T G C A G G A C G T C
AleI  (2531)
1 site		 C A C N N N N G T G G T G N N N N C A C
NotI  (2509)
1 site		 G C G G C C G C C G C C G G C G
BspHI  (2409)
1 site		 T C A T G A A G T A C T
AlwNI  (2105)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
BciVI  (1892)
1 site		 G T A T C C ( N ) 5 N C A T A G G ( N ) 5
The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
DrdI  (1797)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
Acc65I  (14)
1 site		 G G T A C C C C A T G G
KpnI  (18)
1 site		 G G T A C C C C A T G G
Eco53kI  (23)
1 site		 G A G C T C C T C G A G
SacI  (25)
1 site		 G A G C T C C T C G A G
MluI  (29)
1 site		 A C G C G T T G C G C A
NheI  (35)
1 site		 G C T A G C C G A T C G
BmtI  (39)
1 site		 G C T A G C C G A T C G
TspMI  (40)
1 site		 C C C G G G G G G C C C
XmaI  (40)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (42)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
SrfI  (42)
1 site		 G C C C G G G C C G G G C C C G
PaeR7I  (46)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (46)
1 site		 C T C G A G G A G C T C
BglII  (50)
1 site		 A G A T C T T C T A G A
HindIII  (67)
1 site		 A A G C T T T T C G A A
NcoI  (110)
1 site		 C C A T G G G G T A C C
DraIII  (300)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
SphI  (538)
1 site		 G C A T G C C G T A C G
EcoRV  (585)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PfoI  (635)
1 site		 T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BbvCI  (760)
1 site		 C C T C A G C G G A G T C G
XcmI  (761)
1 site		 C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C
The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
PvuII  (764)
1 site		 C A G C T G G T C G A C
BssHII  (843)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
EcoO109I  (957)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
EcoRI  (1044)
1 site		 G A A T T C C T T A A G
XbaI  (1171)
1 site		 T C T A G A A G A T C T
FseI  (1190)
1 site		 G G C C G G C C C C G G C C G G
FseI gradually loses activity when stored at -20°C.
PsiI  (1311)
1 site		 T T A T A A A A T A T T
HpaI  (1331)
1 site		 G T T A A C C A A T T G
MfeI  (1340)
1 site		 C A A T T G G T T A A C
BamHI  (1433)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SalI  (1439)
1 site		 G T C G A C C A G C T G
AccI  (1440)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
PshAI  (1504)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
PciI  (1689)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
Forward Sequencing Primer
23-mer  /  30% GC
1 binding site
3576 .. 3598  =  23 annealed bases
Tm  =  54°C
Reverse Sequencing Primer
22-mer  /  50% GC
1 binding site
184 .. 205  =  22 annealed bases
Tm  =  61°C
Rluc
112 .. 1044  =  933 bp
311 amino acids  =  36.0 kDa
Product: enhanced Renilla luciferase
Rluc
112 .. 1044  =  933 bp
311 amino acids  =  36.0 kDa
Product: enhanced Renilla luciferase
hPEST
1048 .. 1167  =  120 bp
40 amino acids  =  4.2 kDa
Product: PEST degradation sequence from mouse ornithine decarboxylase
human codon-optimized
hPEST
1048 .. 1167  =  120 bp
40 amino acids  =  4.2 kDa
Product: PEST degradation sequence from mouse ornithine decarboxylase
human codon-optimized
AmpR
2538 .. 3398  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   2538 .. 3329  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2538 .. 3398  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   3330 .. 3398  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2538 .. 3398  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ori
1750 .. 2338  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1750 .. 2338  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
SV40 poly(A) signal
1210 .. 1331  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1210 .. 1331  =  122 bp
SV40 polyadenylation signal
pause site
3565 .. 3656  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
pause site
3565 .. 3656  =  92 bp
RNA polymerase II transcriptional pause signal from the human α2 globin gene
MCS
1 .. 72  =  72 bp
multiple cloning site
MCS
1 .. 72  =  72 bp
multiple cloning site
poly(A) signal
3503 .. 3551  =  49 bp
synthetic polyadenylation signal
poly(A) signal
3503 .. 3551  =  49 bp
synthetic polyadenylation signal
ORF:  112 .. 1170  =  1059 bp
ORF:  352 amino acids  =  40.2 kDa
ORF:  2668 .. 2934  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  1296 .. 1538  =  243 bp
ORF:  80 amino acids  =  9.0 kDa
ORF:  2538 .. 3398  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  61 .. 537  =  477 bp
ORF:  158 amino acids  =  16.4 kDa
Click here to try SnapGene

Download pLightSwitch_Prom.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure
Download SnapGene

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps
Download SnapGene Viewer

Individual Sequences & Maps

pBI-CMV5
pGL4.22[luc2CP Puro]
pGL4.81[hRlucCP Neo]
pRL-null
pBIND-ER(alpha)
pGL4.23[luc2 minP]
pGL4.82[hRluc Puro]
pRL-SV40
pBIND-GR
pGL4.24[luc2P minP]
pGL4.83[hRlucP Puro]
pRL-TK
pCBG68-Basic
pGL4.25[luc2CP minP]
pGL4.84[hRlucCP Puro]
pSELECT-zeo-Lucia
pCBG68-Control
pGL4.26[luc2 minP Hygro]
pGLuc Mini-TK 2
pSF-CMV-EMCV-FLuc
pCBG99-Basic
pGL4.27[luc2P minP Hygro]
pGLuc-Basic 2
pSF-CMV-EMCV-RLuc
pCBG99-Control
pGL4.28[luc2CP minP Hygro]
phRG-B
pSF-CMV-FLuc
pCBR-Basic
pGL4.29[luc2P CRE Hygro]
phRG-TK
pSF-CMV-FMDV-FLuc
pCBR-Control
pGL4.30[luc2P NFAT-RE Hygro]
phRL-CMV
pSF-CMV-FMDV-RLuc
pCLuc Mini-TK 2
pGL4.31[luc2P GAL4UAS Hygro]
phRL-null
pSF-CMV-PGK-FLuc
pCLuc-Basic 2
pGL4.32[luc2P NF-kB-RE Hygro]
phRL-SV40
pSF-CMV-PGK-RLuc
pCMV-CLuc 2
pGL4.33[luc2P SRE Hygro]
phRL-TK
pSF-CMV-RLuc
pCMV-Cypridina Luc
pGL4.34[luc2P SRF-RE Hygro]
phRL-TK(Int-)
pSF-CMV-RSV-FLuc AscI
pCMV-Gaussia Luc
pGL4.35[luc2P 9XGAL4UAS Hygro]
pLightSwitch_Prom
pSF-CMV-RSV-RLuc AscI
pCMV-Gaussia-Dura Luc
pGL4.36[luc2P MMTV Hygro]
pLVX-MetLuc Control
pSF-CMV-Ub-FLuc AscI
pCMV-GLuc 2
pGL4.37[luc2P ARE Hygro]
pLVX-MetLuc Reporter
pSF-CMV-Ub-RLuc AscI
pCMV-Green Renilla Luc
pGL4.38[luc2P p53 RE Hygro]
pMCS-Cypridina Luc
pSF-CMVe-FLuc
pCMV-Red Firefly Luc
pGL4.39[luc2P ATF6 RE Hygro]
pMCS-Gaussia Luc
pSF-CMVe-RLuc
pCRE-MetLuc2-Reporter
pGL4.40[luc2P MRE Hygro]
pMCS-Gaussia-Dura Luc
pSF-MinProm-FLuc
pFN26A (BIND) hRluc-neo Flexi Vector
pGL4.41[luc2P HSE Hygro]
pMCS-Green Renilla Luc
pSF-MinProm-RLuc
pGEM-luc
pGL4.42[luc2P HRE Hygro]
pMCS-Red Firefly Luc
pSF-pA-CMVe-FLuc
pGL2-Basic
pGL4.43[luc2P XRE Hygro]
pMetLuc-Mem Control
pSF-pA-CMVe-RLuc
pGL2-Control
pGL4.44[luc2P AP1 RE Hygro]
pMetLuc-Mem Reporter
pSF-pA-MinProm-FLuc
pGL2-Enhancer
pGL4.45[luc2P ISRE Hygro]
pMetLuc2-Control
pSF-pA-MinProm-RLuc
pGL2-Promoter
pGL4.47[luc2P SIE Hygro]
pMetLuc2-Reporter
pSF-pA-PromMCS-FLuc
pGL3-Basic
pGL4.48[luc2P SBE Hygro]
pmirGLO
pSF-pA-PromMCS-RLuc
pGL3-Control
pGL4.49[luc2P TCF-LEF RE Hygro]
pMirTarget
pSF-PromMCS-FLuc
pGL3-Enhancer
pGL4.50[luc2 CMV Hygro]
pNFkB-MetLuc2-Reporter
pSF-PromMCS-RLuc
pGL3-Promoter
pGL4.51[luc2 CMV Neo]
pNL1.1.CMV[Nluc CMV]
pSF-RecA-FLuc
pGL4.10[luc2]
pGL4.52[luc2P STAT5 RE Hygro]
pNL1.1[Nluc]
pSP-luc+NF
pGL4.11[luc2P]
pGL4.70[hRluc]
pNL1.2[NlucP]
pSV40-CLuc
pGL4.12[luc2CP]
pGL4.71[hRlucP]
pNL1.3.CMV[secNluc CMV]
pSV40-GLuc
pGL4.13[luc2 SV40]
pGL4.72[hRlucCP]
pNL1.3[secNluc]
pTK-CLuc
pGL4.14[luc2 Hygro]
pGL4.73[hRluc SV40]
pNL2.1[Nluc Hygro]
pTK-Cypridina Luc
pGL4.15[luc2P Hygro]
pGL4.74[hRluc TK]
pNL2.2[NlucP Hygro]
pTK-Gaussia Luc
pGL4.16[luc2CP Hygro]
pGL4.75[hRluc CMV]
pNL2.3[secNluc Hygro]
pTK-Gaussia-Dura Luc
pGL4.17[luc2 Neo]
pGL4.76[hRluc Hygro]
pNL3.1[Nluc minP]
pTK-GLuc
pGL4.18[luc2P Neo]
pGL4.77[hRlucP Hygro]
pNL3.2.NF-kB-RE[NlucP NF-kB-RE Hygro]
pTK-Green Renilla Luc
pGL4.19[luc2CP Neo]
pGL4.78[hRlucCP Hygro]
pNL3.2[NlucP minP]
pTK-Red Firefly Luc
pGL4.20[luc2 Puro]
pGL4.79[hRluc Neo]
pNL3.3[secNluc minP]
pXPG
pGL4.21[luc2P Puro]
pGL4.80[hRlucP Neo]
pRL-CMV

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.
Try for Free