Azami-Green

Green fluorescent protein from Galaxeidae coral.

Sequence Author: MBL International

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No matches
600 400 200 End (681) DraIII (573) ClaI - BspDI (510) MscI (503) EaeI (501) HpaI - HincII (474) BsiWI - SnaBI (444) BsaWI (390) MslI (325) BmrI (276) Bsu36I (254) EcoP15I (248) PspFI (220) BseYI (216) EcoRV (168) DrdI (130) BsaBI * (28) Start (0) Azami-Green Azami-Green 681 bp
End  (681)
0 sites
DraIII  (573)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
ClaI  (510)
1 site
A T C G A T T A G C T A
BspDI  (510)
1 site
A T C G A T T A G C T A
MscI  (503)
1 site
T G G C C A A C C G G T
EaeI  (501)
1 site
Y G G C C R R C C G G Y
HpaI  (474)
1 site
G T T A A C C A A T T G
HincII  (474)
1 site
G T Y R A C C A R Y T G
BsiWI  (444)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
SnaBI  (444)
1 site
T A C G T A A T G C A T
BsaWI  (390)
1 site
W C C G G W W G G C C W

Cleavage may be enhanced when more than one copy of the BsaWI recognition sequence is present.
MslI  (325)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BmrI  (276)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
Bsu36I  (254)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
EcoP15I  (248)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
PspFI  (220)
1 site
C C C A G C G G G T C G
BseYI  (216)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
EcoRV  (168)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
DrdI  (130)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BsaBI  (28)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
Start  (0)
0 sites
Azami-Green
1 .. 681  =  681 bp
226 amino acids  =  26.1 kDa
Product: Azami-Green fluorescent protein (Karasawa et al., 2003)
Azami-Green
1 .. 681  =  681 bp
226 amino acids  =  26.1 kDa
Product: Azami-Green fluorescent protein (Karasawa et al., 2003)
ORF:  1 .. 681  =  681 bp
ORF:  226 amino acids  =  26.1 kDa
ORF:  121 .. 681  =  561 bp
ORF:  186 amino acids  =  20.8 kDa  (no start codon)
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Download Azami-Green.dna file

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