FAPbeta1

Fluorogen-activating protein composed of the HL1.0.1-TO1 single-chain antibody fragment.

Sequence Author: SpectraGenetics

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No matches
600 400 200 End (771) SmaI (474) XmaI - TspMI (472) Esp3I - BsmBI (369) BstEII (357) AleI (280) BglII (265) BlpI (253) EcoNI (131) BmtI (5) NheI (1) Start (0) FAP β1 FAP β1 771 bp
End  (771)
0 sites
SmaI  (474)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
XmaI  (472)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
TspMI  (472)
1 site
C C C G G G G G G C C C
Esp3I  (369)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different Esp3I sites may not be compatible.
BsmBI  (369)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
BstEII  (357)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
AleI  (280)
1 site
C A C N N N N G T G G T G N N N N C A C
BglII  (265)
1 site
A G A T C T T C T A G A
BlpI  (253)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
EcoNI  (131)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BmtI  (5)
1 site
G C T A G C C G A T C G
NheI  (1)
1 site
G C T A G C C G A T C G
Start  (0)
0 sites
FAPβ1
1 .. 771  =  771 bp
257 amino acids  =  26.4 kDa
Product: fluorogen-activating protein composed of the HL1.0.1-TO1 single-chain antibody fragment (Szent-Gyorgyi et al., 2008)
binds thiazole orange derivatives to produce green fluorescence
FAPβ1
1 .. 771  =  771 bp
257 amino acids  =  26.4 kDa
Product: fluorogen-activating protein composed of the HL1.0.1-TO1 single-chain antibody fragment (Szent-Gyorgyi et al., 2008)
binds thiazole orange derivatives to produce green fluorescence
ORF:  1 .. 771  =  771 bp
ORF:  257 amino acids  =  26.4 kDa  (no start codon)
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Download FAPbeta1.dna file

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