mStayGold (E138D)

Bright and highly photostable monomeric green fluorescent protein created by introducing the E138D mutation into StayGold.
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No matches
600 400 200 End (651) Esp3I - BsmBI (625) TsoI (550) BfuAI - BspMI (549) EarI (533) BlpI (475) BsgI (436) PmlI (430) BmgBI (414) MslI (394) BspHI (308) TatI (266) AvaI - BsoBI (235) BsrBI (125) HindIII (51) Start (0) mStayGold (E138D) mStayGold (E138D) 651 bp
End  (651)
0 sites
Esp3I  (625)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different Esp3I sites may not be compatible.
BsmBI  (625)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
TsoI  (550)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BfuAI  (549)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (549)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
EarI  (533)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
BlpI  (475)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BsgI  (436)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PmlI  (430)
1 site
C A C G T G G T G C A C
BmgBI  (414)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
MslI  (394)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BspHI  (308)
1 site
T C A T G A A G T A C T
TatI  (266)
1 site
W G T A C W W C A T G W
AvaI  (235)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (235)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
BsrBI  (125)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
HindIII  (51)
1 site
A A G C T T T T C G A A
Start  (0)
0 sites
mStayGold (E138D)
1 .. 651  =  651 bp
217 amino acids  =  24.6 kDa
Product: bright and highly photostable monomeric green fluorescent protein (Ivorra-Molla et al., 2023)
contains the monomerizing E138D mutation
mStayGold (E138D)
1 .. 651  =  651 bp
217 amino acids  =  24.6 kDa
Product: bright and highly photostable monomeric green fluorescent protein (Ivorra-Molla et al., 2023)
contains the monomerizing E138D mutation
ORF:  1 .. 651  =  651 bp
ORF:  217 amino acids  =  24.6 kDa
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Download mStayGold (E138D).dna file

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