mClover2

Monomeric derivative of the bright green-yellow fluorescent protein Clover.

Sequence Author: Addgene

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No matches
600 400 200 End (720) BsrGI (710) BlpI (622) Bpu10I (604) AclI (512) MflI * - BstYI (500) BsiEI (464) EagI (461) KpnI (317) Acc65I (313) PfoI * (281) BssSI - BssSαI (181) BsrFI (151) BtgZI (122) SacII (93) MspA1I (92) BtgI (90) BseRI (31) Start (0) mClover2 mClover2 720 bp
End  (720)
0 sites
BsrGI  (710)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BlpI  (622)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
Bpu10I  (604)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
AclI  (512)
1 site
A A C G T T T T G C A A
MflI  (500)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (500)
1 site
R G A T C Y Y C T A G R
BsiEI  (464)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
EagI  (461)
1 site
C G G C C G G C C G G C
KpnI  (317)
1 site
G G T A C C C C A T G G
Acc65I  (313)
1 site
G G T A C C C C A T G G
PfoI  (281)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BssSI  (181)
1 site
C A C G A G G T G C T C
BssSαI  (181)
1 site
C A C G A G G T G C T C
BsrFI  (151)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI  (122)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
SacII  (93)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
MspA1I  (92)
1 site
C M G C K G G K C G M C
BtgI  (90)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BseRI  (31)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
Start  (0)
0 sites
mClover2
1 .. 720  =  720 bp
239 amino acids  =  26.9 kDa
Product: monomeric derivative of the bright green-yellow fluorescent protein Clover
mammalian codon-optimized
mClover2
1 .. 720  =  720 bp
239 amino acids  =  26.9 kDa
Product: monomeric derivative of the bright green-yellow fluorescent protein Clover
mammalian codon-optimized
ORF:  1 .. 720  =  720 bp
ORF:  239 amino acids  =  26.9 kDa
ORF:  3 .. 494  =  492 bp
ORF:  163 amino acids  =  18.8 kDa  (no start codon)
ORF:  1 .. 720  =  720 bp
ORF:  240 amino acids  =  24.2 kDa  (no start codon)
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Download mClover2.dna file

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