cp173Venus

Circularly permuted form of the Venus yellow fluorescent protein. Serves as an efficient FRET acceptor for CFP.
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No matches
600 400 200 End (735) MflI * BstYI (716) PfoI * (497) BtsI - BtsαI (430) PstI (425) SfcI (421) PasI (408) BssSI BssSαI (397) BsrFI (367) BtgZI (338) EaeI (288) BanI (252) BseRI (247) MspA1I (209) BsrGI (194) Start (0) cp173Venus cp173Venus 735 bp
End  (735)
0 sites
MflI  (716)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (716)
1 site
R G A T C Y Y C T A G R
PfoI  (497)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BtsI  (430)
1 site
G C A G T G N N C G T C A C
BtsαI  (430)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
PstI  (425)
1 site
C T G C A G G A C G T C
SfcI  (421)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
PasI  (408)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
BssSI  (397)
1 site
C A C G A G G T G C T C
BssSαI  (397)
1 site
C A C G A G G T G C T C
BsrFI  (367)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI  (338)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
EaeI  (288)
1 site
Y G G C C R R C C G G Y
BanI  (252)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BseRI  (247)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
MspA1I  (209)
1 site
C M G C K G G K C G M C
BsrGI  (194)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
Start  (0)
0 sites
cp173Venus
1 .. 735  =  735 bp
245 amino acids  =  27.3 kDa
Product: circularly permuted form of the Venus yellow fluorescent protein (Nagai et al., 2004)
efficient FRET acceptor for CFP
cp173Venus
1 .. 735  =  735 bp
245 amino acids  =  27.3 kDa
Product: circularly permuted form of the Venus yellow fluorescent protein (Nagai et al., 2004)
efficient FRET acceptor for CFP
ORF:  1 .. 735  =  735 bp
ORF:  245 amino acids  =  27.3 kDa
ORF:  3 .. 734  =  732 bp
ORF:  244 amino acids  =  28.3 kDa  (no start codon)
ORF:  1 .. 735  =  735 bp
ORF:  245 amino acids  =  24.4 kDa  (no start codon)
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Download cp173Venus.dna file

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