PA-TagRFP

Bright photoactivatable variant of the monomeric red fluorescent protein TagRFP.

Sequence Author: Evrogen

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HomePlasmidsFluorescent Protein Genes & PlasmidsPA-TagRFP
600 400 200 End (702) PacI (699) HindIII (691) MscI (655) EaeI (653) DraIII (645) BsaI (616) BsaHI (572) SmlI (559) BpuEI (544) MflI * - BstYI (535) AcuI (505) HincII (474) SalI (472) BglI (464) MspA1I (448) PspFI (420) BseYI (416) NmeAIII (393) PfoI * (329) BpmI (313) BbsI (296) Bsu36I (254) EcoP15I (225) PasI (219) TsoI (163) ApaLI (75) TatI - BsrGI (38) MslI (28) NspI (27) Start (0) PA-TagRFP PA-TagRFP 702 bp
End  (702)
0 sites
PacI  (699)
1 site		 T T A A T T A A A A T T A A T T
HindIII  (691)
1 site		 A A G C T T T T C G A A
MscI  (655)
1 site		 T G G C C A A C C G G T
EaeI  (653)
1 site		 Y G G C C R R C C G G Y
DraIII  (645)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
BsaI  (616)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BsaHI  (572)
1 site		 G R C G Y C C Y G C R G
BsaHI is typically used at 37°C, but is even more active at 60°C.
SmlI  (559)
1 site		 C T Y R A G G A R Y T C
Cleavage may be enhanced when more than one copy of the SmlI recognition sequence is present.
Sticky ends from different SmlI sites may not be compatible.
BpuEI  (544)
1 site		 C T T G A G ( N ) 14 N N G A A C T C ( N ) 14
Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
MflI  (535)
1 site		 R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (535)
1 site		 R G A T C Y Y C T A G R
AcuI  (505)
1 site		 C T G A A G ( N ) 14 N N G A C T T C ( N ) 14
Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
HincII  (474)
1 site		 G T Y R A C C A R Y T G
SalI  (472)
1 site		 G T C G A C C A G C T G
BglI  (464)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
MspA1I  (448)
1 site		 C M G C K G G K C G M C
PspFI  (420)
1 site		 C C C A G C G G G T C G
BseYI  (416)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
NmeAIII  (393)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PfoI  (329)
1 site		 T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BpmI  (313)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BbsI  (296)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
Bsu36I  (254)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
EcoP15I  (225)
1 site		 C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N
Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
PasI  (219)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
TsoI  (163)
1 site		 T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9
Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
ApaLI  (75)
1 site		 G T G C A C C A C G T G
TatI  (38)
1 site		 W G T A C W W C A T G W
BsrGI  (38)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
MslI  (28)
1 site		 C A Y N N N N R T G G T R N N N N Y A C
NspI  (27)
1 site		 R C A T G Y Y G T A C R
Start  (0)
0 sites
PA-TagRFP
1 .. 702  =  702 bp
233 amino acids  =  26.3 kDa
Product: photoactivatable variant of the monomeric red fluorescent protein TagRFP (Subach et al., 2010)
mammalian codon-optimized
PA-TagRFP
1 .. 702  =  702 bp
233 amino acids  =  26.3 kDa
Product: photoactivatable variant of the monomeric red fluorescent protein TagRFP (Subach et al., 2010)
mammalian codon-optimized
ORF:  1 .. 702  =  702 bp
ORF:  233 amino acids  =  26.3 kDa
ORF:  1 .. 702  =  702 bp
ORF:  234 amino acids  =  24.2 kDa  (no start codon)
ORF:  102 .. 347  =  246 bp
ORF:  81 amino acids  =  9.1 kDa
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ptdTomato-C1
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pHcRed1-1
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ecliptic pHluorin
mPlum
pHcRed1-C1
pTurboRFP-B
EGFP
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pHcRed1-N1_1
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mRFP1
phdKeima-Red-S1
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EosFP
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phdKeima570-S1
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hdKeima570
mTagBFP2
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ratiometric pHluorin
hKikGR1
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Rhacostoma GFP
hKO
mTFP1
pKatushka2S-N
rsEGFP
hmAzami-Green
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rsEGFP2
hMGFP
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PA-GFP
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SBFP2
hmKO2
PA-TagRFP
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SCFP3A
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Skylan-NS
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pmCherry-1
SuperNova
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pMFAPalpha2
TagBFP
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PAmCherry1
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TagGFP2
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pmKate2-C
TagRFP
KillerRed
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TagRFP-T
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TagYFP
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YPet
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