Rhacostoma GFP

Dimeric green fluorescent protein from Rhacostoma atlantica.

Sequence Author: Ursa BioScience

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HomePlasmidsFluorescent Protein Genes & PlasmidsRhacostoma GFP
600 400 200 End (717) PsiI (701) Bsu36I (635) BspEI - BsaWI (576) BaeGI - Bme1580I * (509) ApaLI (505) TsoI (429) ApoI - EcoRI (427) MfeI (373) TatI - BsrGI (359) BssSI - BssSαI (326) BsgI (301) AcuI - Eco57MI (290) BsaAI (279) NspI (218) PciI - AflIII (214) MscI (180) EaeI (178) BtgI - StyI - NcoI (175) BsaBI (136) SspI (86) EarI - SapI - BspQI (34) Start (0) Rhacostoma GFP Rhacostoma GFP 717 bp
End  (717)
0 sites
PsiI  (701)
1 site		 T T A T A A A A T A T T
Bsu36I  (635)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
BspEI  (576)
1 site		 T C C G G A A G G C C T
BsaWI  (576)
1 site		 W C C G G W W G G C C W
Cleavage may be enhanced when more than one copy of the BsaWI recognition sequence is present.
BaeGI  (509)
1 site		 G K G C M C C M C G K G
Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (509)
1 site		 G K G C M C C M C G K G
* Blocked by EcoKI methylation.
Sticky ends from different Bme1580I sites may not be compatible.
ApaLI  (505)
1 site		 G T G C A C C A C G T G
TsoI  (429)
1 site		 T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9
Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
ApoI  (427)
1 site		 R A A T T Y Y T T A A R
ApoI is typically used at 50°C, but is 50% active at 37°C.
EcoRI  (427)
1 site		 G A A T T C C T T A A G
MfeI  (373)
1 site		 C A A T T G G T T A A C
TatI  (359)
1 site		 W G T A C W W C A T G W
BsrGI  (359)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
BssSI  (326)
1 site		 C A C G A G G T G C T C
BssSαI  (326)
1 site		 C A C G A G G T G C T C
BsgI  (301)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AcuI  (290)
1 site		 C T G A A G ( N ) 14 N N G A C T T C ( N ) 14
Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (290)
1 site		 C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14
Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaAI  (279)
1 site		 Y A C G T R R T G C A Y
NspI  (218)
1 site		 R C A T G Y Y G T A C R
PciI  (214)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
AflIII  (214)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
MscI  (180)
1 site		 T G G C C A A C C G G T
EaeI  (178)
1 site		 Y G G C C R R C C G G Y
BtgI  (175)
1 site		 C C R Y G G G G Y R C C
Sticky ends from different BtgI sites may not be compatible.
StyI  (175)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
NcoI  (175)
1 site		 C C A T G G G G T A C C
BsaBI  (136)
1 site		 G A T N N N N A T C C T A N N N N T A G
SspI  (86)
1 site		 A A T A T T T T A T A A
EarI  (34)
1 site		 C T C T T C N G A G A A G N N N N
Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
SapI  (34)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BspQI  (34)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
Start  (0)
0 sites
Rhacostoma GFP
1 .. 717  =  717 bp
238 amino acids  =  26.5 kDa
Product: dimeric green fluorescent protein from Rhacostoma atlantica
Rhacostoma GFP
1 .. 717  =  717 bp
238 amino acids  =  26.5 kDa
Product: dimeric green fluorescent protein from Rhacostoma atlantica
ORF:  1 .. 717  =  717 bp
ORF:  238 amino acids  =  26.5 kDa
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ptdTomato-C1
DsRed.T3
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mPapaya1
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ecliptic pHluorin
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pHcRed1-C1
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mRFP1
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EosFP
mRuby
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phKikGR1-S1
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FAPalpha2
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phKO1-S1
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mScarlet
phmAG1-S1
pTurboYFP-N
Fast-FT
mScarlet-2I
phMGFP
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Fluorescent Timer
mScarlet-H
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pZsGreen
FusionRed
mScarlet-I
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Gamillus
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GFP
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GFPuv
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mStayGold(J)
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pZsYellow1-N1
hdKeima570
mTagBFP2
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ratiometric pHluorin
hKikGR1
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Rhacostoma GFP
hKO
mTFP1
pKatushka2S-N
rsEGFP
hmAzami-Green
mTurquoise
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rsEGFP2
hMGFP
mTurquoise2
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rsFusionRed1
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hmKO
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SBFP2
hmKO2
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SCFP3A
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pKindling-Red-B
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pKindling-Red-N
SiriusGFP
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Skylan-NS
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super-ecliptic pHluorin
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pmCherry-1
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pmCherry-C1
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pmCherry-N1
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Kaede
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pMFAPalpha2
TagBFP
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pmKate2-C
TagRFP
KillerRed
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pmKate2-N
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pmKeima-Red-S1
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pmKikGR1-S1
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