pEGFP

Vector for expressing EGFP in bacteria.

Sequence Author: Clontech (TaKaRa)

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HomePlasmidsFluorescent Protein Genes & PlasmidspEGFP
BspQI - SapI (3347) AflIII - PciI (3230) PspFI (2930) BseYI (2926) AlwNI (2821) AhdI (2342) BsaI (2276) FspI (2119) PvuI (1973) TsoI (1944) ScaI (1861) XmnI (1742) PvuII (55) lac operator HindIII (234) BfuAI - BspMI (239) SphI (244) PstI - SbfI (250) SalI (252) AccI (253) HincII (254) BamHI (264) AvaI - BsoBI - TspMI - XmaI (269) SmaI (271) Acc65I (273) AgeI (276) KpnI (277) BtgI - NcoI - StyI (287) BseRI (319) BtgZI (410) BsrGI (998) NotI (1011) ApoI - EcoRI (1025) StuI (1085) SpeI (1089) BsiWI (1099) PspOMI (1104) ApaI - BanII (1108) NdeI (1300) BstAPI (1301) AflII (1357) ZraI (1421) AatII (1423) SspI (1537) pEGFP 3355 bp
BspQI  (3347)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (3347)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AflIII  (3230)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (3230)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
PspFI  (2930)
1 site		 C C C A G C G G G T C G
BseYI  (2926)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AlwNI  (2821)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
AhdI  (2342)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BsaI  (2276)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
FspI  (2119)
1 site		 T G C G C A A C G C G T
PvuI  (1973)
1 site		 C G A T C G G C T A G C
TsoI  (1944)
1 site		 T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9
Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
ScaI  (1861)
1 site		 A G T A C T T C A T G A
XmnI  (1742)
1 site		 G A A N N N N T T C C T T N N N N A A G
PvuII  (55)
1 site		 C A G C T G G T C G A C
HindIII  (234)
1 site		 A A G C T T T T C G A A
BfuAI  (239)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (239)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
SphI  (244)
1 site		 G C A T G C C G T A C G
PstI  (250)
1 site		 C T G C A G G A C G T C
SbfI  (250)
1 site		 C C T G C A G G G G A C G T C C
SalI  (252)
1 site		 G T C G A C C A G C T G
AccI  (253)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
HincII  (254)
1 site		 G T Y R A C C A R Y T G
BamHI  (264)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
AvaI  (269)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BsoBI  (269)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
TspMI  (269)
1 site		 C C C G G G G G G C C C
XmaI  (269)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (271)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
Acc65I  (273)
1 site		 G G T A C C C C A T G G
AgeI  (276)
1 site		 A C C G G T T G G C C A
KpnI  (277)
1 site		 G G T A C C C C A T G G
BtgI  (287)
1 site		 C C R Y G G G G Y R C C
Sticky ends from different BtgI sites may not be compatible.
NcoI  (287)
1 site		 C C A T G G G G T A C C
StyI  (287)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
BseRI  (319)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BtgZI  (410)
1 site		 G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4
Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BsrGI  (998)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
NotI  (1011)
1 site		 G C G G C C G C C G C C G G C G
ApoI  (1025)
1 site		 R A A T T Y Y T T A A R
ApoI is typically used at 50°C, but is 50% active at 37°C.
EcoRI  (1025)
1 site		 G A A T T C C T T A A G
StuI  (1085)
1 site		 A G G C C T T C C G G A
SpeI  (1089)
1 site		 A C T A G T T G A T C A
BsiWI  (1099)
1 site		 C G T A C G G C A T G C
BsiWI is typically used at 55°C, but is 50% active at 37°C.
PspOMI  (1104)
1 site		 G G G C C C C C C G G G
ApaI  (1108)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
BanII  (1108)
1 site		 G R G C Y C C Y C G R G
Sticky ends from different BanII sites may not be compatible.
NdeI  (1300)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
BstAPI  (1301)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
AflII  (1357)
1 site		 C T T A A G G A A T T C
ZraI  (1421)
1 site		 G A C G T C C T G C A G
AatII  (1423)
1 site		 G A C G T C C T G C A G
SspI  (1537)
1 site		 A A T A T T T T A T A A
AmpR
1555 .. 2415  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   1555 .. 1623  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1555 .. 2415  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1624 .. 2415  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1555 .. 2415  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ATG
217 .. 219  =  3 bp
1 amino acid  =  149.2 Da
Product: lacZ start codon
ATG
217 .. 219  =  3 bp
1 amino acid  =  149.2 Da
Product: lacZ start codon
EGFP
289 .. 1008  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 1:  
   289 .. 291  =  3 bp
   1 amino acid  =  149.2 Da
Product: enhanced GFP
mammalian codon-optimized
EGFP
289 .. 1008  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 2:  1a  
   292 .. 294  =  3 bp
   1 amino acid  =  117.1 Da
Product: enhanced GFP
mammalian codon-optimized
EGFP
289 .. 1008  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 3:  
   295 .. 1008  =  714 bp
   237 amino acids  =  26.7 kDa
Product: enhanced GFP
mammalian codon-optimized
EGFP
289 .. 1008  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
Product: enhanced GFP
mammalian codon-optimized
ori
2586 .. 3174  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2586 .. 3174  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
AmpR promoter
1450 .. 1554  =  105 bp
AmpR promoter
1450 .. 1554  =  105 bp
lac promoter
143 .. 173  =  31 bp
3 segments
   Segment 1:  -35  
   143 .. 148  =  6 bp
promoter for the E. coli lac operon
lac promoter
143 .. 173  =  31 bp
3 segments
   Segment 2:  
   149 .. 166  =  18 bp
promoter for the E. coli lac operon
lac promoter
143 .. 173  =  31 bp
3 segments
   Segment 3:  -10  
   167 .. 173  =  7 bp
promoter for the E. coli lac operon
lac promoter
143 .. 173  =  31 bp
3 segments
promoter for the E. coli lac operon
lac operator
181 .. 197  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
181 .. 197  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
ORF:  217 .. 1008  =  792 bp
ORF:  263 amino acids  =  29.6 kDa
ORF:  1555 .. 2415  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  2019 .. 2285  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
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Individual Sequences & Maps

AcGFP1
mCyRFP1
pDsRed-Express2
pPAmCherry1-C1
Azami-Green
mECFP
pDsRed-Express2-1
pPAmCherry1-N1
Azurite BFP
Medium-FT
pDsRed-Express2-C1
pPhi-Yellow-B
BFP
mEGFP
pDsRed-Express2-N1
pPhi-Yellow-C
CFP
mEmerald
pDsRed-Monomer-C In-Fusion Ready
pPhi-Yellow-N
Citrine
mEos2
pDsRed-Monomer-C1
pPhi-Yellow-PRL
Clover
mEos3.1
pDsRed-Monomer-N In-Fusion Ready
pPS-CFP2-C
CopGFP
mEos3.2
pDsRed-Monomer-N1
pPS-CFP2-N
cp173Venus
mEos4a
pDsRed2
pPSmOrange-C1
Cycle 3 GFP
mEos4b
pDsRed2-1
pPSmOrange-N1
CyOFP1
mEYFP
pDsRed2-C1
pRSET-BFP
CyPet
mgfp5
pDsRed2-N1
pRSET-CFP
CyPet (humanized)
mGold
pE2-Crimson
pRSET-EmGFP
CyRFP1
mGreenLantern
pE2-Crimson-C1
PS-CFP2
d1EGFP
mHoneydew
pE2-Crimson-N1
PSmOrange
d2ECFP
MiCy
pECFP
PSmOrange2
d2EGFP
mIFP
pECFP-1
pTagBFP-C
d2EYFP
miniSOG
pECFP-C1
pTagBFP-N
d4EGFP
miRFP670
pECFP-N1
pTagCFP-C
daGFP
miRFP703
pEGFP
pTagCFP-N
Dendra2
miRFP709
pEGFP-1
pTagGFP2-C
dKeima-Red
mKalama1
pEGFP-C1
pTagGFP2-N
dKeima570
mKate2
pEGFP-C2
pTagRFP-C
Dronpa-Green1
mKeima-Red
pEGFP-C3
pTagRFP-N
Dronpa-Green3
mKikGR1
pEGFP-N1
pTagYFP-C
DsRed-Express
mKO
pEGFP-N2
pTagYFP-N
DsRed-Express2
mKO2
pEGFP-N3
ptd-Tomato-N1
DsRed-Max
mMaple
pEYFP
ptdTomato
DsRed-Monomer
mMaroon1
pEYFP-1
ptdTomato-C1
DsRed.T3
mMiCy1
pEYFP-C1
pTimer
DsRed1
mNectarine
pEYFP-N1
pTimer-1
DsRed2
mNeonGreen
pFusionRed-B
pTurboFP602-B
dTomato
mNeptune
pFusionRed-C
pTurboFP602-C
E2-Crimson
mNeptune2
pFusionRed-N
pTurboFP602-N
E2-Orange
mNeptune2.5
pGFP
pTurboFP602-PRL
E2-Red_Green
mOrange
pGFPuv
pTurboGFP-B
EBFP
mOrange2
pGLO
pTurboGFP-C
EBFP2
moxGFP
pHcRed1
pTurboGFP-N
ECFP
mPapaya1
pHcRed1-1
pTurboGFP-PRL
ecliptic pHluorin
mPlum
pHcRed1-C1
pTurboRFP-B
EGFP
mRaspberry
pHcRed1-N1_1
pTurboRFP-C
Emerald GFP
mRFP1
phdKeima-Red-S1
pTurboRFP-N
EosFP
mRuby
phdKeima570-S1
pTurboRFP-PRL
EYFP
mRuby2
phKikGR1-S1
pTurboYFP-B
FAPalpha2
mRuby3
phKO1-S1
pTurboYFP-C
FAPbeta1
mScarlet
phmAG1-S1
pTurboYFP-N
Fast-FT
mScarlet-2I
phMGFP
pTurboYFP-PRL
Fluorescent Timer
mScarlet-H
phmKeima-Red-S1
pZsGreen
FusionRed
mScarlet-I
phmKO1-S1
pZsGreen1-1
Gamillus
mScarlet3
phmUkG1-S1
pZsGreen1-C1
GFP
mseCFP
pHRed
pZsGreen1-N1
GFPuv
msGFP2
pHTomato
pZsYellow
HcRed1
mStayGold (E138D)
pHuji
pZsYellow1-C1
hdKeima-Red
mStayGold(J)
pKaede-S1
pZsYellow1-N1
hdKeima570
mTagBFP2
pKatushka2S-B
ratiometric pHluorin
hKikGR1
mTangerine
pKatushka2S-C
Rhacostoma GFP
hKO
mTFP1
pKatushka2S-N
rsEGFP
hmAzami-Green
mTurquoise
pKikGR1-S1
rsEGFP2
hMGFP
mTurquoise2
pKillerOrange-B
rsFusionRed1
hmKeima-Red
mUkG1
pKillerOrange-C
rsFusionRed2
hmKeima8.5
mVenus
pKillerOrange-N
rsFusionRed3
hmKikGR1
mWasabi
pKillerRed-B
rsTagRFP
hmKO
PA-GFP
pKillerRed-C
SBFP2
hmKO2
PA-TagRFP
pKillerRed-N
SCFP3A
hmMiCy1
pAcGFP1
pKindling-Red-B
SGFP2
hmUkG1
pAcGFP1-1
pKindling-Red-N
SiriusGFP
hrGFP
pAcGFP1-C In-Fusion Ready
pKO1-S1
Skylan-NS
IFP1.1
pAcGFP1-C1
pLSSmOrange-C1
Slow-FT
IFP1.4
pAcGFP1-C2
pLSSmOrange-N1
smURFP
IFP2.0
pAcGFP1-C3
pmAG1-S1
StayGold
iRFP670
pAcGFP1-N In-Fusion Ready
pmBanana
super-ecliptic pHluorin
iRFP682
pAcGFP1-N1
pmCherry
superfolder GFP
iRFP702
pAcGFP1-N2
pmCherry-1
SuperNova
iRFP713
pAcGFP1-N3
pmCherry-C1
SuperNova2
iRFP720
pAG-S1
pmCherry-N1
SYFP2
Kaede
PAmCherry
pMFAPalpha2
TagBFP
Katushka2S
PAmCherry1
pMFAPbeta1
TagCFP
KikGR1
pAmCyan
pMiCy1-S1
TagGFP2
KillerOrange
pAmCyan1-C1
pmKate2-C
TagRFP
KillerRed
pAmCyan1-N1
pmKate2-N
TagRFP-T
Kohinoor
PAmKate
pmKeima-Red-S1
TagRFP657
Kusabira-Orange
pAsRed2
pmKikGR1-S1
TagYFP
LanYFP
pAsRed2-C1
pmKO1-S1
TDsmURFP
LSSmKate1
pAsRed2-N1
pmKO2-S1
tdTomato
LSSmKate2
pd1EGFP-N1
pmMiCy1-S1
TurboFP602
LSSmOrange
pd2ECFP-N1
pmOrange
TurboFP635
mAmetrine
pd2EGFP-N1
pmOrange2
TurboGFP
mAmetrine1.1
pd2EYFP-N1
pmOrange2-C1
TurboRFP
mApple
pd4EGFP-N1
pmOrange2-N1
TurboYFP
mAzami-Green
pDendra2
pmPlum
UnaG
mBaoJin
pDendra2-C
pmRaspberry
Venus
mCardinal
pDendra2-N
pmStrawberry
Y-FAST
mCarmine
pDG1-S1
pmUkG1-S1
yeGFP
mCerulean
pDG3-S1
pNirFP-C
YFP
mCherry
pdKeima-Red-S1
pNirFP-N
YPet
mCherry2
pdKeima570-S1
pPA-TagRFP-C
YPet (humanized)
mCherry2C
pDsRed-Express
pPA-TagRFP-N
ZsGreen
mClavGR2
pDsRed-Express-1
pPAmCherry-C1
ZsGreen1
mClover2
pDsRed-Express-C1
pPAmCherry-N1
ZsYellow1
mClover3
pDsRed-Express-N1

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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