pMFAPalpha2

Vector expressing a plasma membrane-localized version of the fluorogen-activating protein FAPα2.

Sequence Author: SpectraGenetics

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HomePlasmidsFluorescent Protein Genes & PlasmidspMFAPalpha2
PsiI (5950) DraIII (5825) XmnI (5272) ScaI (5153) PvuI (5043) AhdI (4673) SexAI * (4425) StuI (4195) BspDI * - ClaI * (4174) BsaBI * (4157) PluTI (4017) SfoI (4015) NarI (4014) KasI (4013) BclI * (3673) RsrII (3503) BspQI - SapI (3465) BstBI (3338) BssHII - MauBI (1) CMV enhancer SnaBI (365) HindIII (664) Acc65I (670) KpnI (674) BstXI (709) EcoRI (713) EcoRV (729) HA PspOMI (828) ApaI (832) SfiI (836) BlpI (1080) BmgBI (1123) BspEI (1214) SfiI (1569) SacII (1583) PstI (1590) SalI (1592) AccI (1593) BsmI (1633) NotI (1782) AvaI - BmeT110I - BsoBI - PaeR7I - XhoI (1788) BbsI (1994) AflIII - PciI (2100) pMFAP α2 6049 bp
PsiI  (5950)
1 site		 T T A T A A A A T A T T
DraIII  (5825)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
XmnI  (5272)
1 site		 G A A N N N N T T C C T T N N N N A A G
ScaI  (5153)
1 site		 A G T A C T T C A T G A
PvuI  (5043)
1 site		 C G A T C G G C T A G C
AhdI  (4673)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SexAI  (4425)
1 site		 A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
StuI  (4195)
1 site		 A G G C C T T C C G G A
BspDI  (4174)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (4174)
1 site		 A T C G A T T A G C T A
* Blocked by Dam methylation.
BsaBI  (4157)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
PluTI  (4017)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (4015)
1 site		 G G C G C C C C G C G G
NarI  (4014)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (4013)
1 site		 G G C G C C C C G C G G
BclI  (3673)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
RsrII  (3503)
1 site		 C G G W C C G G C C W G G C
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BspQI  (3465)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (3465)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstBI  (3338)
1 site		 T T C G A A A A G C T T
BssHII  (1)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
MauBI  (1)
1 site		 C G C G C G C G G C G C G C G C
SnaBI  (365)
1 site		 T A C G T A A T G C A T
HindIII  (664)
1 site		 A A G C T T T T C G A A
Acc65I  (670)
1 site		 G G T A C C C C A T G G
KpnI  (674)
1 site		 G G T A C C C C A T G G
BstXI  (709)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
EcoRI  (713)
1 site		 G A A T T C C T T A A G
EcoRV  (729)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PspOMI  (828)
1 site		 G G G C C C C C C G G G
ApaI  (832)
1 site		 G G G C C C C C C G G G
ApaI can be used between 25°C and 37°C.
SfiI  (836)
2 sites		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BlpI  (1080)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
BmgBI  (1123)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BspEI  (1214)
1 site		 T C C G G A A G G C C T
SfiI  (1569)
2 sites		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
SacII  (1583)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
PstI  (1590)
1 site		 C T G C A G G A C G T C
SalI  (1592)
1 site		 G T C G A C C A G C T G
AccI  (1593)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BsmI  (1633)
1 site		 G A A T G C N C T T A C G N
Sticky ends from different BsmI sites may not be compatible.
NotI  (1782)
1 site		 G C G G C C G C C G C C G G C G
AvaI  (1788)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BmeT110I  (1788)
1 site		 C Y C G R G G R G C Y C
BsoBI  (1788)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (1788)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1788)
1 site		 C T C G A G G A G C T C
BbsI  (1994)
1 site		 G A A G A C N N C T T C T G N N ( N ) 4
Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
AflIII  (2100)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (2100)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
Igκ leader
737 .. 799  =  63 bp
21 amino acids  =  2.4 kDa
Product: leader sequence from mouse immunoglobulin κ light chain
Igκ leader
737 .. 799  =  63 bp
21 amino acids  =  2.4 kDa
Product: leader sequence from mouse immunoglobulin κ light chain
HA
800 .. 826  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
HA
800 .. 826  =  27 bp
9 amino acids  =  1.1 kDa
Product: HA (human influenza hemagglutinin) epitope tag
FAPα2
842 .. 1561  =  720 bp
240 amino acids  =  ~24.3 kDa
Product: fluorogen-activating protein composed of a dimer of the L5-MG single-chain antibody fragment (Szent-Gyorgyi et al., 2008)
binds malachite green derivatives to produce red fluorescence
FAPα2
842 .. 1561  =  720 bp
240 amino acids  =  ~24.3 kDa
Product: fluorogen-activating protein composed of a dimer of the L5-MG single-chain antibody fragment (Szent-Gyorgyi et al., 2008)
binds malachite green derivatives to produce red fluorescence
Myc
1598 .. 1627  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc proto-oncogene) epitope tag
Myc
1598 .. 1627  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc proto-oncogene) epitope tag
PDGFRβ TM domain
1631 .. 1777  =  147 bp
49 amino acids  =  5.4 kDa
Product: transmembrane domain from platelet derived growth factor receptor beta
PDGFRβ TM domain
1631 .. 1777  =  147 bp
49 amino acids  =  5.4 kDa
Product: transmembrane domain from platelet derived growth factor receptor beta
AmpR
4600 .. 5460  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   4600 .. 5391  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4600 .. 5460  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   5392 .. 5460  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
4600 .. 5460  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR
3357 .. 4145  =  789 bp
262 amino acids  =  28.7 kDa
Product: aminoglycoside phosphotransferase
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
3357 .. 4145  =  789 bp
262 amino acids  =  28.7 kDa
Product: aminoglycoside phosphotransferase
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori
2161 .. 2749  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2161 .. 2749  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
5592 .. 6047  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
5592 .. 6047  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
10 .. 389  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
10 .. 389  =  380 bp
human cytomegalovirus immediate early enhancer
SV40 promoter
4180 .. 4540  =  361 bp
SV40 enhancer and early promoter
SV40 promoter
4180 .. 4540  =  361 bp
SV40 enhancer and early promoter
bGH poly(A) signal
1805 .. 2029  =  225 bp
bovine growth hormone polyadenylation signal
bGH poly(A) signal
1805 .. 2029  =  225 bp
bovine growth hormone polyadenylation signal
CMV promoter
390 .. 593  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
390 .. 593  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
AmpR promoter
5461 .. 5565  =  105 bp
AmpR promoter
5461 .. 5565  =  105 bp
HSV TK poly(A) signal
3078 .. 3125  =  48 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
HSV TK poly(A) signal
3078 .. 3125  =  48 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
T7 promoter
638 .. 656  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
638 .. 656  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
4194 .. 4332  =  139 bp
SV40 origin of replication
SV40 ori
4194 .. 4332  =  139 bp
SV40 origin of replication
ORF:  737 .. 1780  =  1044 bp
ORF:  347 amino acids  =  ~36.2 kDa
ORF:  4730 .. 4996  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  2898 .. 3131  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
ORF:  3306 .. 3836  =  531 bp
ORF:  176 amino acids  =  19.6 kDa
ORF:  3593 .. 3973  =  381 bp
ORF:  126 amino acids  =  14.3 kDa
ORF:  1522 .. 1755  =  234 bp
ORF:  77 amino acids  =  ~8.7 kDa
ORF:  1795 .. 2103  =  309 bp
ORF:  102 amino acids  =  11.3 kDa
ORF:  2887 .. 3336  =  450 bp
ORF:  149 amino acids  =  16.3 kDa
ORF:  4600 .. 5460  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3357 .. 4145  =  789 bp
ORF:  262 amino acids  =  28.7 kDa
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Individual Sequences & Maps

AcGFP1
mCyRFP1
pDsRed-Express2
pPAmCherry1-C1
Azami-Green
mECFP
pDsRed-Express2-1
pPAmCherry1-N1
Azurite BFP
Medium-FT
pDsRed-Express2-C1
pPhi-Yellow-B
BFP
mEGFP
pDsRed-Express2-N1
pPhi-Yellow-C
CFP
mEmerald
pDsRed-Monomer-C In-Fusion Ready
pPhi-Yellow-N
Citrine
mEos2
pDsRed-Monomer-C1
pPhi-Yellow-PRL
Clover
mEos3.1
pDsRed-Monomer-N In-Fusion Ready
pPS-CFP2-C
CopGFP
mEos3.2
pDsRed-Monomer-N1
pPS-CFP2-N
cp173Venus
mEos4a
pDsRed2
pPSmOrange-C1
Cycle 3 GFP
mEos4b
pDsRed2-1
pPSmOrange-N1
CyOFP1
mEYFP
pDsRed2-C1
pRSET-BFP
CyPet
mgfp5
pDsRed2-N1
pRSET-CFP
CyPet (humanized)
mGold
pE2-Crimson
pRSET-EmGFP
CyRFP1
mGreenLantern
pE2-Crimson-C1
PS-CFP2
d1EGFP
mHoneydew
pE2-Crimson-N1
PSmOrange
d2ECFP
MiCy
pECFP
PSmOrange2
d2EGFP
mIFP
pECFP-1
pTagBFP-C
d2EYFP
miniSOG
pECFP-C1
pTagBFP-N
d4EGFP
miRFP670
pECFP-N1
pTagCFP-C
daGFP
miRFP703
pEGFP
pTagCFP-N
Dendra2
miRFP709
pEGFP-1
pTagGFP2-C
dKeima-Red
mKalama1
pEGFP-C1
pTagGFP2-N
dKeima570
mKate2
pEGFP-C2
pTagRFP-C
Dronpa-Green1
mKeima-Red
pEGFP-C3
pTagRFP-N
Dronpa-Green3
mKikGR1
pEGFP-N1
pTagYFP-C
DsRed-Express
mKO
pEGFP-N2
pTagYFP-N
DsRed-Express2
mKO2
pEGFP-N3
ptd-Tomato-N1
DsRed-Max
mMaple
pEYFP
ptdTomato
DsRed-Monomer
mMaroon1
pEYFP-1
ptdTomato-C1
DsRed.T3
mMiCy1
pEYFP-C1
pTimer
DsRed1
mNectarine
pEYFP-N1
pTimer-1
DsRed2
mNeonGreen
pFusionRed-B
pTurboFP602-B
dTomato
mNeptune
pFusionRed-C
pTurboFP602-C
E2-Crimson
mNeptune2
pFusionRed-N
pTurboFP602-N
E2-Orange
mNeptune2.5
pGFP
pTurboFP602-PRL
E2-Red_Green
mOrange
pGFPuv
pTurboGFP-B
EBFP
mOrange2
pGLO
pTurboGFP-C
EBFP2
moxGFP
pHcRed1
pTurboGFP-N
ECFP
mPapaya1
pHcRed1-1
pTurboGFP-PRL
ecliptic pHluorin
mPlum
pHcRed1-C1
pTurboRFP-B
EGFP
mRaspberry
pHcRed1-N1_1
pTurboRFP-C
Emerald GFP
mRFP1
phdKeima-Red-S1
pTurboRFP-N
EosFP
mRuby
phdKeima570-S1
pTurboRFP-PRL
EYFP
mRuby2
phKikGR1-S1
pTurboYFP-B
FAPalpha2
mRuby3
phKO1-S1
pTurboYFP-C
FAPbeta1
mScarlet
phmAG1-S1
pTurboYFP-N
Fast-FT
mScarlet-2I
phMGFP
pTurboYFP-PRL
Fluorescent Timer
mScarlet-H
phmKeima-Red-S1
pZsGreen
FusionRed
mScarlet-I
phmKO1-S1
pZsGreen1-1
Gamillus
mScarlet3
phmUkG1-S1
pZsGreen1-C1
GFP
mseCFP
pHRed
pZsGreen1-N1
GFPuv
msGFP2
pHTomato
pZsYellow
HcRed1
mStayGold (E138D)
pHuji
pZsYellow1-C1
hdKeima-Red
mStayGold(J)
pKaede-S1
pZsYellow1-N1
hdKeima570
mTagBFP2
pKatushka2S-B
ratiometric pHluorin
hKikGR1
mTangerine
pKatushka2S-C
Rhacostoma GFP
hKO
mTFP1
pKatushka2S-N
rsEGFP
hmAzami-Green
mTurquoise
pKikGR1-S1
rsEGFP2
hMGFP
mTurquoise2
pKillerOrange-B
rsFusionRed1
hmKeima-Red
mUkG1
pKillerOrange-C
rsFusionRed2
hmKeima8.5
mVenus
pKillerOrange-N
rsFusionRed3
hmKikGR1
mWasabi
pKillerRed-B
rsTagRFP
hmKO
PA-GFP
pKillerRed-C
SBFP2
hmKO2
PA-TagRFP
pKillerRed-N
SCFP3A
hmMiCy1
pAcGFP1
pKindling-Red-B
SGFP2
hmUkG1
pAcGFP1-1
pKindling-Red-N
SiriusGFP
hrGFP
pAcGFP1-C In-Fusion Ready
pKO1-S1
Skylan-NS
IFP1.1
pAcGFP1-C1
pLSSmOrange-C1
Slow-FT
IFP1.4
pAcGFP1-C2
pLSSmOrange-N1
smURFP
IFP2.0
pAcGFP1-C3
pmAG1-S1
StayGold
iRFP670
pAcGFP1-N In-Fusion Ready
pmBanana
super-ecliptic pHluorin
iRFP682
pAcGFP1-N1
pmCherry
superfolder GFP
iRFP702
pAcGFP1-N2
pmCherry-1
SuperNova
iRFP713
pAcGFP1-N3
pmCherry-C1
SuperNova2
iRFP720
pAG-S1
pmCherry-N1
SYFP2
Kaede
PAmCherry
pMFAPalpha2
TagBFP
Katushka2S
PAmCherry1
pMFAPbeta1
TagCFP
KikGR1
pAmCyan
pMiCy1-S1
TagGFP2
KillerOrange
pAmCyan1-C1
pmKate2-C
TagRFP
KillerRed
pAmCyan1-N1
pmKate2-N
TagRFP-T
Kohinoor
PAmKate
pmKeima-Red-S1
TagRFP657
Kusabira-Orange
pAsRed2
pmKikGR1-S1
TagYFP
LanYFP
pAsRed2-C1
pmKO1-S1
TDsmURFP
LSSmKate1
pAsRed2-N1
pmKO2-S1
tdTomato
LSSmKate2
pd1EGFP-N1
pmMiCy1-S1
TurboFP602
LSSmOrange
pd2ECFP-N1
pmOrange
TurboFP635
mAmetrine
pd2EGFP-N1
pmOrange2
TurboGFP
mAmetrine1.1
pd2EYFP-N1
pmOrange2-C1
TurboRFP
mApple
pd4EGFP-N1
pmOrange2-N1
TurboYFP
mAzami-Green
pDendra2
pmPlum
UnaG
mBaoJin
pDendra2-C
pmRaspberry
Venus
mCardinal
pDendra2-N
pmStrawberry
Y-FAST
mCarmine
pDG1-S1
pmUkG1-S1
yeGFP
mCerulean
pDG3-S1
pNirFP-C
YFP
mCherry
pdKeima-Red-S1
pNirFP-N
YPet
mCherry2
pdKeima570-S1
pPA-TagRFP-C
YPet (humanized)
mCherry2C
pDsRed-Express
pPA-TagRFP-N
ZsGreen
mClavGR2
pDsRed-Express-1
pPAmCherry-C1
ZsGreen1
mClover2
pDsRed-Express-C1
pPAmCherry-N1
ZsYellow1
mClover3
pDsRed-Express-N1

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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