CyPet (humanized)

Cyan fluorescent protein optimized for FRET with YPet and for expression in mammalian cells.
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HomePlasmidsFluorescent Protein Genes & PlasmidsCyPet (humanized)
600 400 200 End (720) BtgI (693) NaeI (684) NgoMIV (682) PpuMI - EcoO109I (630) EcoNI (625) HaeII (620) BsiHKAI (610) PvuII - MspA1I (534) AflIII (449) BsaWI (365) NmeAIII (356) SmaI (328) XmaI - TspMI (326) BsaAI (279) KpnI (225) BanI - Acc65I (221) BtsI - BtsαI (214) BstEII (183) BsaHI (110) BmgBI (66) BstXI (48) Start (0) CyPet CyPet (humanized) 720 bp
End  (720)
0 sites
BtgI  (693)
1 site		 C C R Y G G G G Y R C C
Sticky ends from different BtgI sites may not be compatible.
NaeI  (684)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (682)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
PpuMI  (630)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
EcoO109I  (630)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
EcoNI  (625)
1 site		 C C T N N N N N A G G G G A N N N N N T C C
The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
HaeII  (620)
1 site		 R G C G C Y Y C G C G R
BsiHKAI  (610)
1 site		 G W G C W C C W C G W G
Sticky ends from different BsiHKAI sites may not be compatible.
PvuII  (534)
1 site		 C A G C T G G T C G A C
MspA1I  (534)
1 site		 C M G C K G G K C G M C
AflIII  (449)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
BsaWI  (365)
1 site		 W C C G G W W G G C C W
Cleavage may be enhanced when more than one copy of the BsaWI recognition sequence is present.
NmeAIII  (356)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
SmaI  (328)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
XmaI  (326)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
TspMI  (326)
1 site		 C C C G G G G G G C C C
BsaAI  (279)
1 site		 Y A C G T R R T G C A Y
KpnI  (225)
1 site		 G G T A C C C C A T G G
BanI  (221)
1 site		 G G Y R C C C C R Y G G
Sticky ends from different BanI sites may not be compatible.
Acc65I  (221)
1 site		 G G T A C C C C A T G G
BtsI  (214)
1 site		 G C A G T G N N C G T C A C
BtsαI  (214)
1 site		 G C A G T G N N C G T C A C
Sticky ends from different BtsαI sites may not be compatible.
BstEII  (183)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BsaHI  (110)
1 site		 G R C G Y C C Y G C R G
BsaHI is typically used at 37°C, but is even more active at 60°C.
BmgBI  (66)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BstXI  (48)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
Start  (0)
0 sites
CyPet
1 .. 720  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 1:  
   1 .. 3  =  3 bp
   1 amino acid  =  149.2 Da
Product: cyan fluorescent protein optimized for FRET with YPet (Nguyen and Daugherty, 2005)
mammalian codon-optimized
CyPet
1 .. 720  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 2:  1a  
   4 .. 6  =  3 bp
   1 amino acid  =  117.1 Da
Product: cyan fluorescent protein optimized for FRET with YPet (Nguyen and Daugherty, 2005)
mammalian codon-optimized
CyPet
1 .. 720  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 3:  
   7 .. 720  =  714 bp
   237 amino acids  =  26.6 kDa
Product: cyan fluorescent protein optimized for FRET with YPet (Nguyen and Daugherty, 2005)
mammalian codon-optimized
CyPet
1 .. 720  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
Product: cyan fluorescent protein optimized for FRET with YPet (Nguyen and Daugherty, 2005)
mammalian codon-optimized
ORF:  1 .. 720  =  720 bp
ORF:  239 amino acids  =  26.9 kDa
ORF:  3 .. 719  =  717 bp
ORF:  239 amino acids  =  27.2 kDa  (no start codon)
ORF:  1 .. 720  =  720 bp
ORF:  240 amino acids  =  24.0 kDa  (no start codon)
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Download CyPet (humanized).dna file

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Individual Sequences & Maps

AcGFP1
mCyRFP1
pDsRed-Express2
pPAmCherry1-C1
Azami-Green
mECFP
pDsRed-Express2-1
pPAmCherry1-N1
Azurite BFP
Medium-FT
pDsRed-Express2-C1
pPhi-Yellow-B
BFP
mEGFP
pDsRed-Express2-N1
pPhi-Yellow-C
CFP
mEmerald
pDsRed-Monomer-C In-Fusion Ready
pPhi-Yellow-N
Citrine
mEos2
pDsRed-Monomer-C1
pPhi-Yellow-PRL
Clover
mEos3.1
pDsRed-Monomer-N In-Fusion Ready
pPS-CFP2-C
CopGFP
mEos3.2
pDsRed-Monomer-N1
pPS-CFP2-N
cp173Venus
mEos4a
pDsRed2
pPSmOrange-C1
Cycle 3 GFP
mEos4b
pDsRed2-1
pPSmOrange-N1
CyOFP1
mEYFP
pDsRed2-C1
pRSET-BFP
CyPet
mgfp5
pDsRed2-N1
pRSET-CFP
CyPet (humanized)
mGold
pE2-Crimson
pRSET-EmGFP
CyRFP1
mGreenLantern
pE2-Crimson-C1
PS-CFP2
d1EGFP
mHoneydew
pE2-Crimson-N1
PSmOrange
d2ECFP
MiCy
pECFP
PSmOrange2
d2EGFP
mIFP
pECFP-1
pTagBFP-C
d2EYFP
miniSOG
pECFP-C1
pTagBFP-N
d4EGFP
miRFP670
pECFP-N1
pTagCFP-C
daGFP
miRFP703
pEGFP
pTagCFP-N
Dendra2
miRFP709
pEGFP-1
pTagGFP2-C
dKeima-Red
mKalama1
pEGFP-C1
pTagGFP2-N
dKeima570
mKate2
pEGFP-C2
pTagRFP-C
Dronpa-Green1
mKeima-Red
pEGFP-C3
pTagRFP-N
Dronpa-Green3
mKikGR1
pEGFP-N1
pTagYFP-C
DsRed-Express
mKO
pEGFP-N2
pTagYFP-N
DsRed-Express2
mKO2
pEGFP-N3
ptd-Tomato-N1
DsRed-Max
mMaple
pEYFP
ptdTomato
DsRed-Monomer
mMaroon1
pEYFP-1
ptdTomato-C1
DsRed.T3
mMiCy1
pEYFP-C1
pTimer
DsRed1
mNectarine
pEYFP-N1
pTimer-1
DsRed2
mNeonGreen
pFusionRed-B
pTurboFP602-B
dTomato
mNeptune
pFusionRed-C
pTurboFP602-C
E2-Crimson
mNeptune2
pFusionRed-N
pTurboFP602-N
E2-Orange
mNeptune2.5
pGFP
pTurboFP602-PRL
E2-Red_Green
mOrange
pGFPuv
pTurboGFP-B
EBFP
mOrange2
pGLO
pTurboGFP-C
EBFP2
moxGFP
pHcRed1
pTurboGFP-N
ECFP
mPapaya1
pHcRed1-1
pTurboGFP-PRL
ecliptic pHluorin
mPlum
pHcRed1-C1
pTurboRFP-B
EGFP
mRaspberry
pHcRed1-N1_1
pTurboRFP-C
Emerald GFP
mRFP1
phdKeima-Red-S1
pTurboRFP-N
EosFP
mRuby
phdKeima570-S1
pTurboRFP-PRL
EYFP
mRuby2
phKikGR1-S1
pTurboYFP-B
FAPalpha2
mRuby3
phKO1-S1
pTurboYFP-C
FAPbeta1
mScarlet
phmAG1-S1
pTurboYFP-N
Fast-FT
mScarlet-2I
phMGFP
pTurboYFP-PRL
Fluorescent Timer
mScarlet-H
phmKeima-Red-S1
pZsGreen
FusionRed
mScarlet-I
phmKO1-S1
pZsGreen1-1
Gamillus
mScarlet3
phmUkG1-S1
pZsGreen1-C1
GFP
mseCFP
pHRed
pZsGreen1-N1
GFPuv
msGFP2
pHTomato
pZsYellow
HcRed1
mStayGold (E138D)
pHuji
pZsYellow1-C1
hdKeima-Red
mStayGold(J)
pKaede-S1
pZsYellow1-N1
hdKeima570
mTagBFP2
pKatushka2S-B
ratiometric pHluorin
hKikGR1
mTangerine
pKatushka2S-C
Rhacostoma GFP
hKO
mTFP1
pKatushka2S-N
rsEGFP
hmAzami-Green
mTurquoise
pKikGR1-S1
rsEGFP2
hMGFP
mTurquoise2
pKillerOrange-B
rsFusionRed1
hmKeima-Red
mUkG1
pKillerOrange-C
rsFusionRed2
hmKeima8.5
mVenus
pKillerOrange-N
rsFusionRed3
hmKikGR1
mWasabi
pKillerRed-B
rsTagRFP
hmKO
PA-GFP
pKillerRed-C
SBFP2
hmKO2
PA-TagRFP
pKillerRed-N
SCFP3A
hmMiCy1
pAcGFP1
pKindling-Red-B
SGFP2
hmUkG1
pAcGFP1-1
pKindling-Red-N
SiriusGFP
hrGFP
pAcGFP1-C In-Fusion Ready
pKO1-S1
Skylan-NS
IFP1.1
pAcGFP1-C1
pLSSmOrange-C1
Slow-FT
IFP1.4
pAcGFP1-C2
pLSSmOrange-N1
smURFP
IFP2.0
pAcGFP1-C3
pmAG1-S1
StayGold
iRFP670
pAcGFP1-N In-Fusion Ready
pmBanana
super-ecliptic pHluorin
iRFP682
pAcGFP1-N1
pmCherry
superfolder GFP
iRFP702
pAcGFP1-N2
pmCherry-1
SuperNova
iRFP713
pAcGFP1-N3
pmCherry-C1
SuperNova2
iRFP720
pAG-S1
pmCherry-N1
SYFP2
Kaede
PAmCherry
pMFAPalpha2
TagBFP
Katushka2S
PAmCherry1
pMFAPbeta1
TagCFP
KikGR1
pAmCyan
pMiCy1-S1
TagGFP2
KillerOrange
pAmCyan1-C1
pmKate2-C
TagRFP
KillerRed
pAmCyan1-N1
pmKate2-N
TagRFP-T
Kohinoor
PAmKate
pmKeima-Red-S1
TagRFP657
Kusabira-Orange
pAsRed2
pmKikGR1-S1
TagYFP
LanYFP
pAsRed2-C1
pmKO1-S1
TDsmURFP
LSSmKate1
pAsRed2-N1
pmKO2-S1
tdTomato
LSSmKate2
pd1EGFP-N1
pmMiCy1-S1
TurboFP602
LSSmOrange
pd2ECFP-N1
pmOrange
TurboFP635
mAmetrine
pd2EGFP-N1
pmOrange2
TurboGFP
mAmetrine1.1
pd2EYFP-N1
pmOrange2-C1
TurboRFP
mApple
pd4EGFP-N1
pmOrange2-N1
TurboYFP
mAzami-Green
pDendra2
pmPlum
UnaG
mBaoJin
pDendra2-C
pmRaspberry
Venus
mCardinal
pDendra2-N
pmStrawberry
Y-FAST
mCarmine
pDG1-S1
pmUkG1-S1
yeGFP
mCerulean
pDG3-S1
pNirFP-C
YFP
mCherry
pdKeima-Red-S1
pNirFP-N
YPet
mCherry2
pdKeima570-S1
pPA-TagRFP-C
YPet (humanized)
mCherry2C
pDsRed-Express
pPA-TagRFP-N
ZsGreen
mClavGR2
pDsRed-Express-1
pPAmCherry-C1
ZsGreen1
mClover2
pDsRed-Express-C1
pPAmCherry-N1
ZsYellow1
mClover3
pDsRed-Express-N1

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