ratiometric pHluorin

GFP mutant that responds to a drop in pH with a reversible decrease in excitation at 395 nm and a corresponding increase in excitation at 475 nm.
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HomePlasmidsFluorescent Protein Genes & Plasmidsratiometric pHluorin
600 400 200 End (717) PspFI (683) BpuEI (682) BseYI - AlwNI (679) PvuII - MspA1I (676) SmlI (661) DrdI (652) EcoP15I (649) BsaI (638) MflI * - BstYI (627) BstBI (622) BfuAI - BspMI (608) BsgI (586) BtgZI (581) MfeI (560) KpnI (482) BanI - Acc65I (478) DraIII (447) BsiHKAI (443) DraI (390) AcuI (350) PmlI - BsaAI (325) AflIII (322) NdeI (230) MscI (171) EaeI (169) BtgI - StyI - NcoI (166) BaeGI - Bme1580I (74) BpmI (47) Start (0) ratiometric pHluorin ratiometric pHluorin 717 bp
End  (717)
0 sites
PspFI  (683)
1 site		 C C C A G C G G G T C G
BpuEI  (682)
1 site		 C T T G A G ( N ) 14 N N G A A C T C ( N ) 14
Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BseYI  (679)
1 site		 C C C A G C G G G T C G
After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AlwNI  (679)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
PvuII  (676)
1 site		 C A G C T G G T C G A C
MspA1I  (676)
1 site		 C M G C K G G K C G M C
SmlI  (661)
1 site		 C T Y R A G G A R Y T C
Cleavage may be enhanced when more than one copy of the SmlI recognition sequence is present.
Sticky ends from different SmlI sites may not be compatible.
DrdI  (652)
1 site		 G A C N N N N N N G T C C T G N N N N N N C A G
Sticky ends from different DrdI sites may not be compatible.
EcoP15I  (649)
1 site		 C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N
Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
BsaI  (638)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
MflI  (627)
1 site		 R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (627)
1 site		 R G A T C Y Y C T A G R
BstBI  (622)
1 site		 T T C G A A A A G C T T
BfuAI  (608)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (608)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BsgI  (586)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BtgZI  (581)
1 site		 G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4
Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
MfeI  (560)
1 site		 C A A T T G G T T A A C
KpnI  (482)
1 site		 G G T A C C C C A T G G
BanI  (478)
1 site		 G G Y R C C C C R Y G G
Sticky ends from different BanI sites may not be compatible.
Acc65I  (478)
1 site		 G G T A C C C C A T G G
DraIII  (447)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
BsiHKAI  (443)
1 site		 G W G C W C C W C G W G
Sticky ends from different BsiHKAI sites may not be compatible.
DraI  (390)
1 site		 T T T A A A A A A T T T
AcuI  (350)
1 site		 C T G A A G ( N ) 14 N N G A C T T C ( N ) 14
Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
PmlI  (325)
1 site		 C A C G T G G T G C A C
BsaAI  (325)
1 site		 Y A C G T R R T G C A Y
AflIII  (322)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
NdeI  (230)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
MscI  (171)
1 site		 T G G C C A A C C G G T
EaeI  (169)
1 site		 Y G G C C R R C C G G Y
BtgI  (166)
1 site		 C C R Y G G G G Y R C C
Sticky ends from different BtgI sites may not be compatible.
StyI  (166)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
NcoI  (166)
1 site		 C C A T G G G G T A C C
BaeGI  (74)
1 site		 G K G C M C C M C G K G
Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (74)
1 site		 G K G C M C C M C G K G
Sticky ends from different Bme1580I sites may not be compatible.
BpmI  (47)
1 site		 C T G G A G ( N ) 14 N N G A C C T C ( N ) 14
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
Start  (0)
0 sites
ratiometric pHluorin
1 .. 717  =  717 bp
238 amino acids  =  26.9 kDa
Product: pH-sensitive mutant of green fluorescent protein (Miesenböck et al., 1998)
responds to a drop in pH with a reversible decrease in excitation at 395 nm and a corresponding increase in excitation at 475 nm
ratiometric pHluorin
1 .. 717  =  717 bp
238 amino acids  =  26.9 kDa
Product: pH-sensitive mutant of green fluorescent protein (Miesenböck et al., 1998)
responds to a drop in pH with a reversible decrease in excitation at 395 nm and a corresponding increase in excitation at 475 nm
ORF:  1 .. 717  =  717 bp
ORF:  238 amino acids  =  26.9 kDa
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DsRed-Express2
mKO2
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ptd-Tomato-N1
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mMaple
pEYFP
ptdTomato
DsRed-Monomer
mMaroon1
pEYFP-1
ptdTomato-C1
DsRed.T3
mMiCy1
pEYFP-C1
pTimer
DsRed1
mNectarine
pEYFP-N1
pTimer-1
DsRed2
mNeonGreen
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pTurboFP602-B
dTomato
mNeptune
pFusionRed-C
pTurboFP602-C
E2-Crimson
mNeptune2
pFusionRed-N
pTurboFP602-N
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mNeptune2.5
pGFP
pTurboFP602-PRL
E2-Red_Green
mOrange
pGFPuv
pTurboGFP-B
EBFP
mOrange2
pGLO
pTurboGFP-C
EBFP2
moxGFP
pHcRed1
pTurboGFP-N
ECFP
mPapaya1
pHcRed1-1
pTurboGFP-PRL
ecliptic pHluorin
mPlum
pHcRed1-C1
pTurboRFP-B
EGFP
mRaspberry
pHcRed1-N1_1
pTurboRFP-C
Emerald GFP
mRFP1
phdKeima-Red-S1
pTurboRFP-N
EosFP
mRuby
phdKeima570-S1
pTurboRFP-PRL
EYFP
mRuby2
phKikGR1-S1
pTurboYFP-B
FAPalpha2
mRuby3
phKO1-S1
pTurboYFP-C
FAPbeta1
mScarlet
phmAG1-S1
pTurboYFP-N
Fast-FT
mScarlet-2I
phMGFP
pTurboYFP-PRL
Fluorescent Timer
mScarlet-H
phmKeima-Red-S1
pZsGreen
FusionRed
mScarlet-I
phmKO1-S1
pZsGreen1-1
Gamillus
mScarlet3
phmUkG1-S1
pZsGreen1-C1
GFP
mseCFP
pHRed
pZsGreen1-N1
GFPuv
msGFP2
pHTomato
pZsYellow
HcRed1
mStayGold (E138D)
pHuji
pZsYellow1-C1
hdKeima-Red
mStayGold(J)
pKaede-S1
pZsYellow1-N1
hdKeima570
mTagBFP2
pKatushka2S-B
ratiometric pHluorin
hKikGR1
mTangerine
pKatushka2S-C
Rhacostoma GFP
hKO
mTFP1
pKatushka2S-N
rsEGFP
hmAzami-Green
mTurquoise
pKikGR1-S1
rsEGFP2
hMGFP
mTurquoise2
pKillerOrange-B
rsFusionRed1
hmKeima-Red
mUkG1
pKillerOrange-C
rsFusionRed2
hmKeima8.5
mVenus
pKillerOrange-N
rsFusionRed3
hmKikGR1
mWasabi
pKillerRed-B
rsTagRFP
hmKO
PA-GFP
pKillerRed-C
SBFP2
hmKO2
PA-TagRFP
pKillerRed-N
SCFP3A
hmMiCy1
pAcGFP1
pKindling-Red-B
SGFP2
hmUkG1
pAcGFP1-1
pKindling-Red-N
SiriusGFP
hrGFP
pAcGFP1-C In-Fusion Ready
pKO1-S1
Skylan-NS
IFP1.1
pAcGFP1-C1
pLSSmOrange-C1
Slow-FT
IFP1.4
pAcGFP1-C2
pLSSmOrange-N1
smURFP
IFP2.0
pAcGFP1-C3
pmAG1-S1
StayGold
iRFP670
pAcGFP1-N In-Fusion Ready
pmBanana
super-ecliptic pHluorin
iRFP682
pAcGFP1-N1
pmCherry
superfolder GFP
iRFP702
pAcGFP1-N2
pmCherry-1
SuperNova
iRFP713
pAcGFP1-N3
pmCherry-C1
SuperNova2
iRFP720
pAG-S1
pmCherry-N1
SYFP2
Kaede
PAmCherry
pMFAPalpha2
TagBFP
Katushka2S
PAmCherry1
pMFAPbeta1
TagCFP
KikGR1
pAmCyan
pMiCy1-S1
TagGFP2
KillerOrange
pAmCyan1-C1
pmKate2-C
TagRFP
KillerRed
pAmCyan1-N1
pmKate2-N
TagRFP-T
Kohinoor
PAmKate
pmKeima-Red-S1
TagRFP657
Kusabira-Orange
pAsRed2
pmKikGR1-S1
TagYFP
LanYFP
pAsRed2-C1
pmKO1-S1
TDsmURFP
LSSmKate1
pAsRed2-N1
pmKO2-S1
tdTomato
LSSmKate2
pd1EGFP-N1
pmMiCy1-S1
TurboFP602
LSSmOrange
pd2ECFP-N1
pmOrange
TurboFP635
mAmetrine
pd2EGFP-N1
pmOrange2
TurboGFP
mAmetrine1.1
pd2EYFP-N1
pmOrange2-C1
TurboRFP
mApple
pd4EGFP-N1
pmOrange2-N1
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mAzami-Green
pDendra2
pmPlum
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pDendra2-C
pmRaspberry
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pmStrawberry
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pmUkG1-S1
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YPet
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