pTurboRFP-B

Vector for expressing TurboRFP in bacteria.

Sequence Author: Evrogen

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HomePlasmidsFluorescent Protein Genes & PlasmidspTurboRFP-B
EcoO109I (4086) AatII (4032) ZraI (4030) XmnI (3709) PvuI (3480) FspI (3332) NmeAIII (3258) BsrFI (3190) AhdI (3110) AlwNI (2633) AflIII - PciI (2217) BspQI - SapI (2101) BstAPI (2041) AvaI - BsoBI - PaeR7I - XhoI (1) PsiI (49) MfeI (59) lac operator ATG BseRI (115) BsaBI * (125) BamHI (126) BclI * (142) BsrGI (169) Bsu36I (385) SfiI (595) BsgI (624) BfuAI - BspMI (646) DraIII (713) PshAI (759) HindIII (827) BlpI (839) NheI (947) BmtI (951) Bpu10I (972) PvuII (1099) BspEI (1195) BtgI - NcoI - StyI (1500) XbaI (1804) PfoI (1861) PflFI - Tth111I (1964) BsaAI (1971) AccI (1989) BstZ17I (1990) NdeI (2040) pTurboRFP-B 4102 bp
EcoO109I  (4086)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
AatII  (4032)
1 site		 G A C G T C C T G C A G
ZraI  (4030)
1 site		 G A C G T C C T G C A G
XmnI  (3709)
1 site		 G A A N N N N T T C C T T N N N N A A G
PvuI  (3480)
1 site		 C G A T C G G C T A G C
FspI  (3332)
1 site		 T G C G C A A C G C G T
NmeAIII  (3258)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsrFI  (3190)
1 site		 R C C G G Y Y G G C C R
Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
AhdI  (3110)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2633)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
AflIII  (2217)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
PciI  (2217)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
BspQI  (2101)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different BspQI sites may not be compatible.
SapI  (2101)
1 site		 G C T C T T C N C G A G A A G N N N N
Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstAPI  (2041)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
AvaI  (1)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BsoBI  (1)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (1)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site		 C T C G A G G A G C T C
PsiI  (49)
1 site		 T T A T A A A A T A T T
MfeI  (59)
1 site		 C A A T T G G T T A A C
BseRI  (115)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BsaBI  (125)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BamHI  (126)
1 site		 G G A T C C C C T A G G
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BclI  (142)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BsrGI  (169)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
Bsu36I  (385)
1 site		 C C T N A G G G G A N T C C
Sticky ends from different Bsu36I sites may not be compatible.
SfiI  (595)
1 site		 G G C C N N N N N G G C C C C G G N N N N N C C G G
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BsgI  (624)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BfuAI  (646)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (646)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
DraIII  (713)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
PshAI  (759)
1 site		 G A C N N N N G T C C T G N N N N C A G
PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
HindIII  (827)
1 site		 A A G C T T T T C G A A
BlpI  (839)
1 site		 G C T N A G C C G A N T C G
Sticky ends from different BlpI sites may not be compatible.
NheI  (947)
1 site		 G C T A G C C G A T C G
BmtI  (951)
1 site		 G C T A G C C G A T C G
Bpu10I  (972)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PvuII  (1099)
1 site		 C A G C T G G T C G A C
BspEI  (1195)
1 site		 T C C G G A A G G C C T
BtgI  (1500)
1 site		 C C R Y G G G G Y R C C
Sticky ends from different BtgI sites may not be compatible.
NcoI  (1500)
1 site		 C C A T G G G G T A C C
StyI  (1500)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
XbaI  (1804)
1 site		 T C T A G A A G A T C T
PfoI  (1861)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
PflFI  (1964)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1964)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsaAI  (1971)
1 site		 Y A C G T R R T G C A Y
AccI  (1989)
1 site		 G T M K A C C A K M T G
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BstZ17I  (1990)
1 site		 G T A T A C C A T A T G
NdeI  (2040)
1 site		 C A T A T G G T A T A C
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
AmpR
3037 .. 3897  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   3037 .. 3828  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3037 .. 3897  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   3829 .. 3897  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3037 .. 3897  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ATG
114 .. 116  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
114 .. 116  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
TurboRFP
132 .. 827  =  696 bp
231 amino acids  =  26.1 kDa
Product: red fluorescent protein from Entacmaea quadricolor
mammalian codon-optimized
TurboRFP
132 .. 827  =  696 bp
231 amino acids  =  26.1 kDa
Product: red fluorescent protein from Entacmaea quadricolor
mammalian codon-optimized
CmR
986 .. 1645  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
986 .. 1645  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
2278 .. 2866  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2278 .. 2866  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
AmpR promoter
3898 .. 4002  =  105 bp
AmpR promoter
3898 .. 4002  =  105 bp
lambda t0 terminator
848 .. 942  =  95 bp
transcription terminator from phage lambda
lambda t0 terminator
848 .. 942  =  95 bp
transcription terminator from phage lambda
rrnB T1 terminator
1710 .. 1796  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
1710 .. 1796  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
T5 promoter
10 .. 54  =  45 bp
4 segments
   Segment 1:  
   10 .. 24  =  15 bp
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
T5 promoter
10 .. 54  =  45 bp
4 segments
   Segment 2:  -35  
   25 .. 30  =  6 bp
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
T5 promoter
10 .. 54  =  45 bp
4 segments
   Segment 3:  
   31 .. 47  =  17 bp
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
T5 promoter
10 .. 54  =  45 bp
4 segments
   Segment 4:  -10  
   48 .. 54  =  7 bp
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
T5 promoter
10 .. 54  =  45 bp
4 segments
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
lac operator
62 .. 78  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
62 .. 78  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
30 .. 46  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
30 .. 46  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
ORF:  986 .. 1645  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  3167 .. 3433  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  114 .. 827  =  714 bp
ORF:  237 amino acids  =  26.7 kDa
ORF:  233 .. 478  =  246 bp
ORF:  81 amino acids  =  9.1 kDa
ORF:  3037 .. 3897  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
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Individual Sequences & Maps

AcGFP1
mCyRFP1
pDsRed-Express2
pPAmCherry1-C1
Azami-Green
mECFP
pDsRed-Express2-1
pPAmCherry1-N1
Azurite BFP
Medium-FT
pDsRed-Express2-C1
pPhi-Yellow-B
BFP
mEGFP
pDsRed-Express2-N1
pPhi-Yellow-C
CFP
mEmerald
pDsRed-Monomer-C In-Fusion Ready
pPhi-Yellow-N
Citrine
mEos2
pDsRed-Monomer-C1
pPhi-Yellow-PRL
Clover
mEos3.1
pDsRed-Monomer-N In-Fusion Ready
pPS-CFP2-C
CopGFP
mEos3.2
pDsRed-Monomer-N1
pPS-CFP2-N
cp173Venus
mEos4a
pDsRed2
pPSmOrange-C1
Cycle 3 GFP
mEos4b
pDsRed2-1
pPSmOrange-N1
CyOFP1
mEYFP
pDsRed2-C1
pRSET-BFP
CyPet
mgfp5
pDsRed2-N1
pRSET-CFP
CyPet (humanized)
mGold
pE2-Crimson
pRSET-EmGFP
CyRFP1
mGreenLantern
pE2-Crimson-C1
PS-CFP2
d1EGFP
mHoneydew
pE2-Crimson-N1
PSmOrange
d2ECFP
MiCy
pECFP
PSmOrange2
d2EGFP
mIFP
pECFP-1
pTagBFP-C
d2EYFP
miniSOG
pECFP-C1
pTagBFP-N
d4EGFP
miRFP670
pECFP-N1
pTagCFP-C
daGFP
miRFP703
pEGFP
pTagCFP-N
Dendra2
miRFP709
pEGFP-1
pTagGFP2-C
dKeima-Red
mKalama1
pEGFP-C1
pTagGFP2-N
dKeima570
mKate2
pEGFP-C2
pTagRFP-C
Dronpa-Green1
mKeima-Red
pEGFP-C3
pTagRFP-N
Dronpa-Green3
mKikGR1
pEGFP-N1
pTagYFP-C
DsRed-Express
mKO
pEGFP-N2
pTagYFP-N
DsRed-Express2
mKO2
pEGFP-N3
ptd-Tomato-N1
DsRed-Max
mMaple
pEYFP
ptdTomato
DsRed-Monomer
mMaroon1
pEYFP-1
ptdTomato-C1
DsRed.T3
mMiCy1
pEYFP-C1
pTimer
DsRed1
mNectarine
pEYFP-N1
pTimer-1
DsRed2
mNeonGreen
pFusionRed-B
pTurboFP602-B
dTomato
mNeptune
pFusionRed-C
pTurboFP602-C
E2-Crimson
mNeptune2
pFusionRed-N
pTurboFP602-N
E2-Orange
mNeptune2.5
pGFP
pTurboFP602-PRL
E2-Red_Green
mOrange
pGFPuv
pTurboGFP-B
EBFP
mOrange2
pGLO
pTurboGFP-C
EBFP2
moxGFP
pHcRed1
pTurboGFP-N
ECFP
mPapaya1
pHcRed1-1
pTurboGFP-PRL
ecliptic pHluorin
mPlum
pHcRed1-C1
pTurboRFP-B
EGFP
mRaspberry
pHcRed1-N1_1
pTurboRFP-C
Emerald GFP
mRFP1
phdKeima-Red-S1
pTurboRFP-N
EosFP
mRuby
phdKeima570-S1
pTurboRFP-PRL
EYFP
mRuby2
phKikGR1-S1
pTurboYFP-B
FAPalpha2
mRuby3
phKO1-S1
pTurboYFP-C
FAPbeta1
mScarlet
phmAG1-S1
pTurboYFP-N
Fast-FT
mScarlet-2I
phMGFP
pTurboYFP-PRL
Fluorescent Timer
mScarlet-H
phmKeima-Red-S1
pZsGreen
FusionRed
mScarlet-I
phmKO1-S1
pZsGreen1-1
Gamillus
mScarlet3
phmUkG1-S1
pZsGreen1-C1
GFP
mseCFP
pHRed
pZsGreen1-N1
GFPuv
msGFP2
pHTomato
pZsYellow
HcRed1
mStayGold (E138D)
pHuji
pZsYellow1-C1
hdKeima-Red
mStayGold(J)
pKaede-S1
pZsYellow1-N1
hdKeima570
mTagBFP2
pKatushka2S-B
ratiometric pHluorin
hKikGR1
mTangerine
pKatushka2S-C
Rhacostoma GFP
hKO
mTFP1
pKatushka2S-N
rsEGFP
hmAzami-Green
mTurquoise
pKikGR1-S1
rsEGFP2
hMGFP
mTurquoise2
pKillerOrange-B
rsFusionRed1
hmKeima-Red
mUkG1
pKillerOrange-C
rsFusionRed2
hmKeima8.5
mVenus
pKillerOrange-N
rsFusionRed3
hmKikGR1
mWasabi
pKillerRed-B
rsTagRFP
hmKO
PA-GFP
pKillerRed-C
SBFP2
hmKO2
PA-TagRFP
pKillerRed-N
SCFP3A
hmMiCy1
pAcGFP1
pKindling-Red-B
SGFP2
hmUkG1
pAcGFP1-1
pKindling-Red-N
SiriusGFP
hrGFP
pAcGFP1-C In-Fusion Ready
pKO1-S1
Skylan-NS
IFP1.1
pAcGFP1-C1
pLSSmOrange-C1
Slow-FT
IFP1.4
pAcGFP1-C2
pLSSmOrange-N1
smURFP
IFP2.0
pAcGFP1-C3
pmAG1-S1
StayGold
iRFP670
pAcGFP1-N In-Fusion Ready
pmBanana
super-ecliptic pHluorin
iRFP682
pAcGFP1-N1
pmCherry
superfolder GFP
iRFP702
pAcGFP1-N2
pmCherry-1
SuperNova
iRFP713
pAcGFP1-N3
pmCherry-C1
SuperNova2
iRFP720
pAG-S1
pmCherry-N1
SYFP2
Kaede
PAmCherry
pMFAPalpha2
TagBFP
Katushka2S
PAmCherry1
pMFAPbeta1
TagCFP
KikGR1
pAmCyan
pMiCy1-S1
TagGFP2
KillerOrange
pAmCyan1-C1
pmKate2-C
TagRFP
KillerRed
pAmCyan1-N1
pmKate2-N
TagRFP-T
Kohinoor
PAmKate
pmKeima-Red-S1
TagRFP657
Kusabira-Orange
pAsRed2
pmKikGR1-S1
TagYFP
LanYFP
pAsRed2-C1
pmKO1-S1
TDsmURFP
LSSmKate1
pAsRed2-N1
pmKO2-S1
tdTomato
LSSmKate2
pd1EGFP-N1
pmMiCy1-S1
TurboFP602
LSSmOrange
pd2ECFP-N1
pmOrange
TurboFP635
mAmetrine
pd2EGFP-N1
pmOrange2
TurboGFP
mAmetrine1.1
pd2EYFP-N1
pmOrange2-C1
TurboRFP
mApple
pd4EGFP-N1
pmOrange2-N1
TurboYFP
mAzami-Green
pDendra2
pmPlum
UnaG
mBaoJin
pDendra2-C
pmRaspberry
Venus
mCardinal
pDendra2-N
pmStrawberry
Y-FAST
mCarmine
pDG1-S1
pmUkG1-S1
yeGFP
mCerulean
pDG3-S1
pNirFP-C
YFP
mCherry
pdKeima-Red-S1
pNirFP-N
YPet
mCherry2
pdKeima570-S1
pPA-TagRFP-C
YPet (humanized)
mCherry2C
pDsRed-Express
pPA-TagRFP-N
ZsGreen
mClavGR2
pDsRed-Express-1
pPAmCherry-C1
ZsGreen1
mClover2
pDsRed-Express-C1
pPAmCherry-N1
ZsYellow1
mClover3
pDsRed-Express-N1

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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