TagGFP2

Monomeric green fluorescent protein. Also known as mTagGFP.

Sequence Author: Evrogen

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HomePlasmidsFluorescent Protein Genes & PlasmidsTagGFP2
600 400 200 End (717) AlwNI (677) EcoP15I (650) Bpu10I (601) BsaBI * (586) PasI (561) PvuII (531) AflIII (446) EciI (445) SacII (327) XmnI (244) BstAPI (206) BstEII (180) BsaHI (107) ApaLI (64) BmgBI (63) BseRI (28) NaeI (27) NgoMIV (25) BsrBI (5) Start (0) TagGFP2 TagGFP2 717 bp
End  (717)
0 sites
AlwNI  (677)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
EcoP15I  (650)
1 site		 C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N
Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
Bpu10I  (601)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsaBI  (586)
1 site		 G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
PasI  (561)
1 site		 C C C W G G G G G G W C C C
Sticky ends from different PasI sites may not be compatible.
PvuII  (531)
1 site		 C A G C T G G T C G A C
AflIII  (446)
1 site		 A C R Y G T T G Y R C A
Sticky ends from different AflIII sites may not be compatible.
EciI  (445)
1 site		 G G C G G A ( N ) 9 N N C C G C C T ( N ) 9
Sticky ends from different EciI sites may not be compatible.
SacII  (327)
1 site		 C C G C G G G G C G C C
Efficient cleavage requires at least two copies of the SacII recognition sequence.
XmnI  (244)
1 site		 G A A N N N N T T C C T T N N N N A A G
BstAPI  (206)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
BstEII  (180)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BsaHI  (107)
1 site		 G R C G Y C C Y G C R G
BsaHI is typically used at 37°C, but is even more active at 60°C.
ApaLI  (64)
1 site		 G T G C A C C A C G T G
BmgBI  (63)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BseRI  (28)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
NaeI  (27)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (25)
1 site		 G C C G G C C G G C C G
Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
BsrBI  (5)
1 site		 C C G C T C G G C G A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
Start  (0)
0 sites
TagGFP2
1 .. 717  =  717 bp
238 amino acids  =  26.9 kDa
Product: monomeric green fluorescent protein, also known as mTagGFP
mammalian codon-optimized
TagGFP2
1 .. 717  =  717 bp
238 amino acids  =  26.9 kDa
Product: monomeric green fluorescent protein, also known as mTagGFP
mammalian codon-optimized
ORF:  1 .. 717  =  717 bp
ORF:  238 amino acids  =  26.9 kDa
ORF:  3 .. 491  =  489 bp
ORF:  162 amino acids  =  18.5 kDa  (no start codon)
ORF:  1 .. 717  =  717 bp
ORF:  239 amino acids  =  23.6 kDa  (no start codon)
ORF:  273 .. 512  =  240 bp
ORF:  79 amino acids  =  9.4 kDa
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Individual Sequences & Maps

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mIFP
pECFP-1
pTagBFP-C
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miniSOG
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miRFP670
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daGFP
miRFP703
pEGFP
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miRFP709
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mKikGR1
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pTagYFP-N
DsRed-Express2
mKO2
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mMaple
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ptdTomato
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ptdTomato-C1
DsRed.T3
mMiCy1
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pTimer
DsRed1
mNectarine
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pTimer-1
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pTurboFP602-B
dTomato
mNeptune
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mNeptune2
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pGFP
pTurboFP602-PRL
E2-Red_Green
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pGFPuv
pTurboGFP-B
EBFP
mOrange2
pGLO
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EBFP2
moxGFP
pHcRed1
pTurboGFP-N
ECFP
mPapaya1
pHcRed1-1
pTurboGFP-PRL
ecliptic pHluorin
mPlum
pHcRed1-C1
pTurboRFP-B
EGFP
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pHcRed1-N1_1
pTurboRFP-C
Emerald GFP
mRFP1
phdKeima-Red-S1
pTurboRFP-N
EosFP
mRuby
phdKeima570-S1
pTurboRFP-PRL
EYFP
mRuby2
phKikGR1-S1
pTurboYFP-B
FAPalpha2
mRuby3
phKO1-S1
pTurboYFP-C
FAPbeta1
mScarlet
phmAG1-S1
pTurboYFP-N
Fast-FT
mScarlet-2I
phMGFP
pTurboYFP-PRL
Fluorescent Timer
mScarlet-H
phmKeima-Red-S1
pZsGreen
FusionRed
mScarlet-I
phmKO1-S1
pZsGreen1-1
Gamillus
mScarlet3
phmUkG1-S1
pZsGreen1-C1
GFP
mseCFP
pHRed
pZsGreen1-N1
GFPuv
msGFP2
pHTomato
pZsYellow
HcRed1
mStayGold (E138D)
pHuji
pZsYellow1-C1
hdKeima-Red
mStayGold(J)
pKaede-S1
pZsYellow1-N1
hdKeima570
mTagBFP2
pKatushka2S-B
ratiometric pHluorin
hKikGR1
mTangerine
pKatushka2S-C
Rhacostoma GFP
hKO
mTFP1
pKatushka2S-N
rsEGFP
hmAzami-Green
mTurquoise
pKikGR1-S1
rsEGFP2
hMGFP
mTurquoise2
pKillerOrange-B
rsFusionRed1
hmKeima-Red
mUkG1
pKillerOrange-C
rsFusionRed2
hmKeima8.5
mVenus
pKillerOrange-N
rsFusionRed3
hmKikGR1
mWasabi
pKillerRed-B
rsTagRFP
hmKO
PA-GFP
pKillerRed-C
SBFP2
hmKO2
PA-TagRFP
pKillerRed-N
SCFP3A
hmMiCy1
pAcGFP1
pKindling-Red-B
SGFP2
hmUkG1
pAcGFP1-1
pKindling-Red-N
SiriusGFP
hrGFP
pAcGFP1-C In-Fusion Ready
pKO1-S1
Skylan-NS
IFP1.1
pAcGFP1-C1
pLSSmOrange-C1
Slow-FT
IFP1.4
pAcGFP1-C2
pLSSmOrange-N1
smURFP
IFP2.0
pAcGFP1-C3
pmAG1-S1
StayGold
iRFP670
pAcGFP1-N In-Fusion Ready
pmBanana
super-ecliptic pHluorin
iRFP682
pAcGFP1-N1
pmCherry
superfolder GFP
iRFP702
pAcGFP1-N2
pmCherry-1
SuperNova
iRFP713
pAcGFP1-N3
pmCherry-C1
SuperNova2
iRFP720
pAG-S1
pmCherry-N1
SYFP2
Kaede
PAmCherry
pMFAPalpha2
TagBFP
Katushka2S
PAmCherry1
pMFAPbeta1
TagCFP
KikGR1
pAmCyan
pMiCy1-S1
TagGFP2
KillerOrange
pAmCyan1-C1
pmKate2-C
TagRFP
KillerRed
pAmCyan1-N1
pmKate2-N
TagRFP-T
Kohinoor
PAmKate
pmKeima-Red-S1
TagRFP657
Kusabira-Orange
pAsRed2
pmKikGR1-S1
TagYFP
LanYFP
pAsRed2-C1
pmKO1-S1
TDsmURFP
LSSmKate1
pAsRed2-N1
pmKO2-S1
tdTomato
LSSmKate2
pd1EGFP-N1
pmMiCy1-S1
TurboFP602
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pd2ECFP-N1
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TurboFP635
mAmetrine
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pd2EYFP-N1
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pd4EGFP-N1
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pmRaspberry
Venus
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Y-FAST
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pmUkG1-S1
yeGFP
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YFP
mCherry
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pNirFP-N
YPet
mCherry2
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pPA-TagRFP-C
YPet (humanized)
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pPAmCherry-C1
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