ZsYellow1

Zoanthus yellow fluorescent protein.

Sequence Author: Clontech (TaKaRa)

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HomePlasmidsFluorescent Protein Genes & PlasmidsZsYellow1
600 400 200 End (696) BstXI (687) TaqII (673) BsiHKAI (665) BtgZI (654) BstAPI (609) AvaI - BsoBI (583) BaeGI - Bme1580I (572) TatI - BsrGI (551) KpnI (531) AgeI (530) Acc65I (527) EagI (522) BfuAI - BspMI (512) NspI (501) BmgBI (492) BmrI (441) PflMI (431) PluTI (308) SfoI (306) NarI (305) KasI (304) MflI * - BstYI (209) SgrAI (88) BclI * (83) BseRI (43) Start (0) ZsYellow1 ZsYellow1 696 bp
End  (696)
0 sites
BstXI  (687)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
TaqII  (673)
1 site		 G A C C G A ( N ) 9 N N C T G G C T ( N ) 9
Sticky ends from different TaqII sites may not be compatible.
BsiHKAI  (665)
1 site		 G W G C W C C W C G W G
Sticky ends from different BsiHKAI sites may not be compatible.
BtgZI  (654)
1 site		 G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4
Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BstAPI  (609)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
AvaI  (583)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different AvaI sites may not be compatible.
BsoBI  (583)
1 site		 C Y C G R G G R G C Y C
Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
BaeGI  (572)
1 site		 G K G C M C C M C G K G
Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (572)
1 site		 G K G C M C C M C G K G
Sticky ends from different Bme1580I sites may not be compatible.
TatI  (551)
1 site		 W G T A C W W C A T G W
BsrGI  (551)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
KpnI  (531)
1 site		 G G T A C C C C A T G G
AgeI  (530)
1 site		 A C C G G T T G G C C A
Acc65I  (527)
1 site		 G G T A C C C C A T G G
EagI  (522)
1 site		 C G G C C G G C C G G C
BfuAI  (512)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (512)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
NspI  (501)
1 site		 R C A T G Y Y G T A C R
BmgBI  (492)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BmrI  (441)
1 site		 A C T G G G ( N ) 4 N T G A C C C ( N ) 4
The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
PflMI  (431)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
PluTI  (308)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (306)
1 site		 G G C G C C C C G C G G
NarI  (305)
1 site		 G G C G C C C C G C G G
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (304)
1 site		 G G C G C C C C G C G G
MflI  (209)
1 site		 R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (209)
1 site		 R G A T C Y Y C T A G R
SgrAI  (88)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BclI  (83)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BseRI  (43)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
Start  (0)
0 sites
ZsYellow1
1 .. 696  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus yellow fluorescent protein
mammalian codon-optimized
ZsYellow1
1 .. 696  =  696 bp
231 amino acids  =  26.1 kDa
Product: Zoanthus yellow fluorescent protein
mammalian codon-optimized
ORF:  1 .. 696  =  696 bp
ORF:  231 amino acids  =  26.1 kDa
ORF:  3 .. 323  =  321 bp
ORF:  106 amino acids  =  12.3 kDa  (no start codon)
ORF:  1 .. 696  =  696 bp
ORF:  232 amino acids  =  23.0 kDa  (no start codon)
ORF:  360 .. 695  =  336 bp
ORF:  111 amino acids  =  12.5 kDa  (no start codon)
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Individual Sequences & Maps

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ptd-Tomato-N1
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ptdTomato
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ptdTomato-C1
DsRed.T3
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pEYFP-C1
pTimer
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mNectarine
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pTimer-1
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pFusionRed-B
pTurboFP602-B
dTomato
mNeptune
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pTurboFP602-C
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mNeptune2
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pTurboFP602-N
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pGFP
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E2-Red_Green
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pGFPuv
pTurboGFP-B
EBFP
mOrange2
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EBFP2
moxGFP
pHcRed1
pTurboGFP-N
ECFP
mPapaya1
pHcRed1-1
pTurboGFP-PRL
ecliptic pHluorin
mPlum
pHcRed1-C1
pTurboRFP-B
EGFP
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pHcRed1-N1_1
pTurboRFP-C
Emerald GFP
mRFP1
phdKeima-Red-S1
pTurboRFP-N
EosFP
mRuby
phdKeima570-S1
pTurboRFP-PRL
EYFP
mRuby2
phKikGR1-S1
pTurboYFP-B
FAPalpha2
mRuby3
phKO1-S1
pTurboYFP-C
FAPbeta1
mScarlet
phmAG1-S1
pTurboYFP-N
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phMGFP
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Fluorescent Timer
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phmKeima-Red-S1
pZsGreen
FusionRed
mScarlet-I
phmKO1-S1
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Gamillus
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phmUkG1-S1
pZsGreen1-C1
GFP
mseCFP
pHRed
pZsGreen1-N1
GFPuv
msGFP2
pHTomato
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HcRed1
mStayGold (E138D)
pHuji
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hdKeima-Red
mStayGold(J)
pKaede-S1
pZsYellow1-N1
hdKeima570
mTagBFP2
pKatushka2S-B
ratiometric pHluorin
hKikGR1
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pKatushka2S-C
Rhacostoma GFP
hKO
mTFP1
pKatushka2S-N
rsEGFP
hmAzami-Green
mTurquoise
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rsEGFP2
hMGFP
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rsFusionRed1
hmKeima-Red
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pKillerOrange-C
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hmKO
PA-GFP
pKillerRed-C
SBFP2
hmKO2
PA-TagRFP
pKillerRed-N
SCFP3A
hmMiCy1
pAcGFP1
pKindling-Red-B
SGFP2
hmUkG1
pAcGFP1-1
pKindling-Red-N
SiriusGFP
hrGFP
pAcGFP1-C In-Fusion Ready
pKO1-S1
Skylan-NS
IFP1.1
pAcGFP1-C1
pLSSmOrange-C1
Slow-FT
IFP1.4
pAcGFP1-C2
pLSSmOrange-N1
smURFP
IFP2.0
pAcGFP1-C3
pmAG1-S1
StayGold
iRFP670
pAcGFP1-N In-Fusion Ready
pmBanana
super-ecliptic pHluorin
iRFP682
pAcGFP1-N1
pmCherry
superfolder GFP
iRFP702
pAcGFP1-N2
pmCherry-1
SuperNova
iRFP713
pAcGFP1-N3
pmCherry-C1
SuperNova2
iRFP720
pAG-S1
pmCherry-N1
SYFP2
Kaede
PAmCherry
pMFAPalpha2
TagBFP
Katushka2S
PAmCherry1
pMFAPbeta1
TagCFP
KikGR1
pAmCyan
pMiCy1-S1
TagGFP2
KillerOrange
pAmCyan1-C1
pmKate2-C
TagRFP
KillerRed
pAmCyan1-N1
pmKate2-N
TagRFP-T
Kohinoor
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pmKeima-Red-S1
TagRFP657
Kusabira-Orange
pAsRed2
pmKikGR1-S1
TagYFP
LanYFP
pAsRed2-C1
pmKO1-S1
TDsmURFP
LSSmKate1
pAsRed2-N1
pmKO2-S1
tdTomato
LSSmKate2
pd1EGFP-N1
pmMiCy1-S1
TurboFP602
LSSmOrange
pd2ECFP-N1
pmOrange
TurboFP635
mAmetrine
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mAmetrine1.1
pd2EYFP-N1
pmOrange2-C1
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pmStrawberry
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pmUkG1-S1
yeGFP
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pNirFP-C
YFP
mCherry
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pNirFP-N
YPet
mCherry2
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