pGLO

Bacterial transformation plasmid for regulated expression of GFP.

Sequence Author: Bio-Rad

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HomePlasmidsFluorescent Protein Genes & PlasmidspGLO
BspDI - ClaI (2) SgrAI (5307) AfeI (5225) BsmBI - Esp3I (5105) PfoI (5103) PflFI - Tth111I (5004) PciI (4748) AlwNI (4339) PsiI (3896) DraIII (3771) AhdI (3423) BsaI (3357) BglI (3305) NmeAIII (3276) AseI (3248) FspI (3200) PvuI (3054) ScaI (2942) NsiI (9) BsaBI (241) AflII (301) EcoRV (386) BssHII (670) NruI (708) PflMI (920) AgeI (1074) BstEII (1077) BsmI (1097) NheI (1345) BmtI (1349) NcoI - StyI (1510) MscI (1515) BsrGI (1620) PaeR7I - PspXI - XhoI (1765) BstBI (1966) EcoRI (2063) Acc65I (2075) KpnI - TspMI - XmaI (2079) SmaI (2081) XbaI (2090) SbfI (2106) BfuAI - BspMI (2109) SphI (2112) KflI - PpuMI (2286) pGLO 5371 bp
BspDI  (2)
1 site		 A T C G A T T A G C T A
ClaI  (2)
1 site		 A T C G A T T A G C T A
SgrAI  (5307)
1 site		 C R C C G G Y G G Y G G C C R C
Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AfeI  (5225)
1 site		 A G C G C T T C G C G A
BsmBI  (5105)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (5105)
1 site		 C G T C T C N G C A G A G N ( N ) 4
Sticky ends from different Esp3I sites may not be compatible.
PfoI  (5103)
1 site		 T C C N G G A A G G N C C T
Sticky ends from different PfoI sites may not be compatible.
PflFI  (5004)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (5004)
1 site		 G A C N N N G T C C T G N N N C A G
The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PciI  (4748)
1 site		 A C A T G T T G T A C A
PciI is inhibited by nonionic detergents.
AlwNI  (4339)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
PsiI  (3896)
1 site		 T T A T A A A A T A T T
DraIII  (3771)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
AhdI  (3423)
1 site		 G A C N N N N N G T C C T G N N N N N C A G
The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BsaI  (3357)
1 site		 G G T C T C N C C A G A G N ( N ) 4
Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BglI  (3305)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
NmeAIII  (3276)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AseI  (3248)
1 site		 A T T A A T T A A T T A
FspI  (3200)
1 site		 T G C G C A A C G C G T
PvuI  (3054)
1 site		 C G A T C G G C T A G C
ScaI  (2942)
1 site		 A G T A C T T C A T G A
NsiI  (9)
1 site		 A T G C A T T A C G T A
BsaBI  (241)
1 site		 G A T N N N N A T C C T A N N N N T A G
AflII  (301)
1 site		 C T T A A G G A A T T C
EcoRV  (386)
1 site		 G A T A T C C T A T A G
EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BssHII  (670)
1 site		 G C G C G C C G C G C G
BssHII is typically used at 50°C, but is 75% active at 37°C.
NruI  (708)
1 site		 T C G C G A A G C G C T
PflMI  (920)
1 site		 C C A N N N N N T G G G G T N N N N N A C C
Sticky ends from different PflMI sites may not be compatible.
AgeI  (1074)
1 site		 A C C G G T T G G C C A
BstEII  (1077)
1 site		 G G T N A C C C C A N T G G
Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BsmI  (1097)
1 site		 G A A T G C N C T T A C G N
Sticky ends from different BsmI sites may not be compatible.
NheI  (1345)
1 site		 G C T A G C C G A T C G
BmtI  (1349)
1 site		 G C T A G C C G A T C G
NcoI  (1510)
1 site		 C C A T G G G G T A C C
StyI  (1510)
1 site		 C C W W G G G G W W C C
Sticky ends from different StyI sites may not be compatible.
MscI  (1515)
1 site		 T G G C C A A C C G G T
BsrGI  (1620)
1 site		 T G T A C A A C A T G T
BsrGI is typically used at 37°C, but is even more active at 60°C.
PaeR7I  (1765)
1 site		 C T C G A G G A G C T C
PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1765)
1 site		 V C T C G A G B B G A G C T C V
XhoI  (1765)
1 site		 C T C G A G G A G C T C
BstBI  (1966)
1 site		 T T C G A A A A G C T T
EcoRI  (2063)
1 site		 G A A T T C C T T A A G
Acc65I  (2075)
1 site		 G G T A C C C C A T G G
KpnI  (2079)
1 site		 G G T A C C C C A T G G
TspMI  (2079)
1 site		 C C C G G G G G G C C C
XmaI  (2079)
1 site		 C C C G G G G G G C C C
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (2081)
1 site		 C C C G G G G G G C C C
SmaI can be used at 37°C for brief incubations.
XbaI  (2090)
1 site		 T C T A G A A G A T C T
SbfI  (2106)
1 site		 C C T G C A G G G G A C G T C C
BfuAI  (2109)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (2109)
1 site		 A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4
Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
SphI  (2112)
1 site		 G C A T G C C G T A C G
KflI  (2286)
1 site		 G G G W C C C C C C W G G G
Sticky ends from different KflI sites may not be compatible.
PpuMI  (2286)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
araC
96 .. 974  =  879 bp
292 amino acids  =  33.4 kDa
Product: L-arabinose regulatory protein
araC
96 .. 974  =  879 bp
292 amino acids  =  33.4 kDa
Product: L-arabinose regulatory protein
AmpR
2636 .. 3496  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   2636 .. 2704  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2636 .. 3496  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   2705 .. 3496  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2636 .. 3496  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
GFP
1342 .. 2061  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 1:  
   1342 .. 1344  =  3 bp
   1 amino acid  =  149.2 Da
Product: Aequoria victoria green fluorescent protein
GFP
1342 .. 2061  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 2:  1a  
   1345 .. 1347  =  3 bp
   1 amino acid  =  89.1 Da
Product: Aequoria victoria green fluorescent protein
GFP
1342 .. 2061  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 3:  
   1348 .. 2061  =  714 bp
   237 amino acids  =  26.7 kDa
Product: Aequoria victoria green fluorescent protein
GFP
1342 .. 2061  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
Product: Aequoria victoria green fluorescent protein
ori
4104 .. 4692  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4104 .. 4692  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
3538 .. 3993  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
3538 .. 3993  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
araBAD promoter
1120 .. 1285  =  166 bp
promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction
araBAD promoter
1120 .. 1285  =  166 bp
promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction
AmpR promoter
2544 .. 2635  =  92 bp
AmpR promoter
2544 .. 2635  =  92 bp
rrnB T1 terminator
2321 .. 2407  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
2321 .. 2407  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
MCS
2063 .. 2119  =  57 bp
pUC18/19 multiple cloning site
MCS
2063 .. 2119  =  57 bp
pUC18/19 multiple cloning site
ORF:  1342 .. 2061  =  720 bp
ORF:  239 amino acids  =  26.9 kDa
ORF:  2636 .. 3496  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
ORF:  660 .. 908  =  249 bp
ORF:  82 amino acids  =  9.3 kDa
ORF:  5115 .. 109  =  366 bp
ORF:  121 amino acids  =  12.8 kDa
ORF:  2249 .. 2488  =  240 bp
ORF:  79 amino acids  =  8.6 kDa
ORF:  1927 .. 2295  =  369 bp
ORF:  122 amino acids  =  13.6 kDa
ORF:  3100 .. 3366  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  96 .. 1025  =  930 bp
ORF:  309 amino acids  =  35.2 kDa
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Individual Sequences & Maps

AcGFP1
mCyRFP1
pDsRed-Express2
pPAmCherry1-C1
Azami-Green
mECFP
pDsRed-Express2-1
pPAmCherry1-N1
Azurite BFP
Medium-FT
pDsRed-Express2-C1
pPhi-Yellow-B
BFP
mEGFP
pDsRed-Express2-N1
pPhi-Yellow-C
CFP
mEmerald
pDsRed-Monomer-C In-Fusion Ready
pPhi-Yellow-N
Citrine
mEos2
pDsRed-Monomer-C1
pPhi-Yellow-PRL
Clover
mEos3.1
pDsRed-Monomer-N In-Fusion Ready
pPS-CFP2-C
CopGFP
mEos3.2
pDsRed-Monomer-N1
pPS-CFP2-N
cp173Venus
mEos4a
pDsRed2
pPSmOrange-C1
Cycle 3 GFP
mEos4b
pDsRed2-1
pPSmOrange-N1
CyOFP1
mEYFP
pDsRed2-C1
pRSET-BFP
CyPet
mgfp5
pDsRed2-N1
pRSET-CFP
CyPet (humanized)
mGold
pE2-Crimson
pRSET-EmGFP
CyRFP1
mGreenLantern
pE2-Crimson-C1
PS-CFP2
d1EGFP
mHoneydew
pE2-Crimson-N1
PSmOrange
d2ECFP
MiCy
pECFP
PSmOrange2
d2EGFP
mIFP
pECFP-1
pTagBFP-C
d2EYFP
miniSOG
pECFP-C1
pTagBFP-N
d4EGFP
miRFP670
pECFP-N1
pTagCFP-C
daGFP
miRFP703
pEGFP
pTagCFP-N
Dendra2
miRFP709
pEGFP-1
pTagGFP2-C
dKeima-Red
mKalama1
pEGFP-C1
pTagGFP2-N
dKeima570
mKate2
pEGFP-C2
pTagRFP-C
Dronpa-Green1
mKeima-Red
pEGFP-C3
pTagRFP-N
Dronpa-Green3
mKikGR1
pEGFP-N1
pTagYFP-C
DsRed-Express
mKO
pEGFP-N2
pTagYFP-N
DsRed-Express2
mKO2
pEGFP-N3
ptd-Tomato-N1
DsRed-Max
mMaple
pEYFP
ptdTomato
DsRed-Monomer
mMaroon1
pEYFP-1
ptdTomato-C1
DsRed.T3
mMiCy1
pEYFP-C1
pTimer
DsRed1
mNectarine
pEYFP-N1
pTimer-1
DsRed2
mNeonGreen
pFusionRed-B
pTurboFP602-B
dTomato
mNeptune
pFusionRed-C
pTurboFP602-C
E2-Crimson
mNeptune2
pFusionRed-N
pTurboFP602-N
E2-Orange
mNeptune2.5
pGFP
pTurboFP602-PRL
E2-Red_Green
mOrange
pGFPuv
pTurboGFP-B
EBFP
mOrange2
pGLO
pTurboGFP-C
EBFP2
moxGFP
pHcRed1
pTurboGFP-N
ECFP
mPapaya1
pHcRed1-1
pTurboGFP-PRL
ecliptic pHluorin
mPlum
pHcRed1-C1
pTurboRFP-B
EGFP
mRaspberry
pHcRed1-N1_1
pTurboRFP-C
Emerald GFP
mRFP1
phdKeima-Red-S1
pTurboRFP-N
EosFP
mRuby
phdKeima570-S1
pTurboRFP-PRL
EYFP
mRuby2
phKikGR1-S1
pTurboYFP-B
FAPalpha2
mRuby3
phKO1-S1
pTurboYFP-C
FAPbeta1
mScarlet
phmAG1-S1
pTurboYFP-N
Fast-FT
mScarlet-2I
phMGFP
pTurboYFP-PRL
Fluorescent Timer
mScarlet-H
phmKeima-Red-S1
pZsGreen
FusionRed
mScarlet-I
phmKO1-S1
pZsGreen1-1
Gamillus
mScarlet3
phmUkG1-S1
pZsGreen1-C1
GFP
mseCFP
pHRed
pZsGreen1-N1
GFPuv
msGFP2
pHTomato
pZsYellow
HcRed1
mStayGold (E138D)
pHuji
pZsYellow1-C1
hdKeima-Red
mStayGold(J)
pKaede-S1
pZsYellow1-N1
hdKeima570
mTagBFP2
pKatushka2S-B
ratiometric pHluorin
hKikGR1
mTangerine
pKatushka2S-C
Rhacostoma GFP
hKO
mTFP1
pKatushka2S-N
rsEGFP
hmAzami-Green
mTurquoise
pKikGR1-S1
rsEGFP2
hMGFP
mTurquoise2
pKillerOrange-B
rsFusionRed1
hmKeima-Red
mUkG1
pKillerOrange-C
rsFusionRed2
hmKeima8.5
mVenus
pKillerOrange-N
rsFusionRed3
hmKikGR1
mWasabi
pKillerRed-B
rsTagRFP
hmKO
PA-GFP
pKillerRed-C
SBFP2
hmKO2
PA-TagRFP
pKillerRed-N
SCFP3A
hmMiCy1
pAcGFP1
pKindling-Red-B
SGFP2
hmUkG1
pAcGFP1-1
pKindling-Red-N
SiriusGFP
hrGFP
pAcGFP1-C In-Fusion Ready
pKO1-S1
Skylan-NS
IFP1.1
pAcGFP1-C1
pLSSmOrange-C1
Slow-FT
IFP1.4
pAcGFP1-C2
pLSSmOrange-N1
smURFP
IFP2.0
pAcGFP1-C3
pmAG1-S1
StayGold
iRFP670
pAcGFP1-N In-Fusion Ready
pmBanana
super-ecliptic pHluorin
iRFP682
pAcGFP1-N1
pmCherry
superfolder GFP
iRFP702
pAcGFP1-N2
pmCherry-1
SuperNova
iRFP713
pAcGFP1-N3
pmCherry-C1
SuperNova2
iRFP720
pAG-S1
pmCherry-N1
SYFP2
Kaede
PAmCherry
pMFAPalpha2
TagBFP
Katushka2S
PAmCherry1
pMFAPbeta1
TagCFP
KikGR1
pAmCyan
pMiCy1-S1
TagGFP2
KillerOrange
pAmCyan1-C1
pmKate2-C
TagRFP
KillerRed
pAmCyan1-N1
pmKate2-N
TagRFP-T
Kohinoor
PAmKate
pmKeima-Red-S1
TagRFP657
Kusabira-Orange
pAsRed2
pmKikGR1-S1
TagYFP
LanYFP
pAsRed2-C1
pmKO1-S1
TDsmURFP
LSSmKate1
pAsRed2-N1
pmKO2-S1
tdTomato
LSSmKate2
pd1EGFP-N1
pmMiCy1-S1
TurboFP602
LSSmOrange
pd2ECFP-N1
pmOrange
TurboFP635
mAmetrine
pd2EGFP-N1
pmOrange2
TurboGFP
mAmetrine1.1
pd2EYFP-N1
pmOrange2-C1
TurboRFP
mApple
pd4EGFP-N1
pmOrange2-N1
TurboYFP
mAzami-Green
pDendra2
pmPlum
UnaG
mBaoJin
pDendra2-C
pmRaspberry
Venus
mCardinal
pDendra2-N
pmStrawberry
Y-FAST
mCarmine
pDG1-S1
pmUkG1-S1
yeGFP
mCerulean
pDG3-S1
pNirFP-C
YFP
mCherry
pdKeima-Red-S1
pNirFP-N
YPet
mCherry2
pdKeima570-S1
pPA-TagRFP-C
YPet (humanized)
mCherry2C
pDsRed-Express
pPA-TagRFP-N
ZsGreen
mClavGR2
pDsRed-Express-1
pPAmCherry-C1
ZsGreen1
mClover2
pDsRed-Express-C1
pPAmCherry-N1
ZsYellow1
mClover3
pDsRed-Express-N1

Plasmid Sets

Basic Cloning Vectors
I.M.A.G.E. Consortium Plasmids
pET & Duet Vectors (Novagen)
TA and GC Cloning Vectors
Coronavirus Resources
Insect Cell Vectors
pGEX Vectors (GE Healthcare)
TOPO Cloning Vectors
CRISPR Plasmids
Luciferase Vectors
Plant Vectors
Viral Expression & Packaging Vectors
Fluorescent Protein Genes & Plasmids
Lucigen Vectors
Qiagen Vectors
Yeast Plasmids
Gateway® Cloning Vectors
Mammalian Expression Vectors
Structural Genomics Vectors

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