hmMiCy1

Humanized monomeric Midoriishi-Cyan fluorescent protein.

Sequence Author: MBL International

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HomePlasmidsFluorescent Protein Genes & PlasmidshmMiCy1
600 400 200 End (699) NmeAIII (689) BseRI (670) DraIII (591) BstXI (588) BsgI (523) AlwNI (501) BmrI - BmgBI (489) BbvCI - Bpu10I (478) BglI (350) EciI (331) PpuMI EcoO109I (293) StuI (265) BstAPI (218) BclI * (143) BsrFI - BaeGI - Bme1580I (103) BssSI - BssSαI (76) FspI - FspAI (40) BtgZI (9) Start (0) hmMiCy1 hmMiCy1 699 bp
End  (699)
0 sites
NmeAIII  (689)
1 site		 G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19
Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BseRI  (670)
1 site		 G A G G A G ( N ) 8 N N C T C C T C ( N ) 8
Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
DraIII  (591)
1 site		 C A C N N N G T G G T G N N N C A C
Sticky ends from different DraIII sites may not be compatible.
BstXI  (588)
1 site		 C C A N N N N N N T G G G G T N N N N N N A C C
Sticky ends from different BstXI sites may not be compatible.
BsgI  (523)
1 site		 G T G C A G ( N ) 14 N N C A C G T C ( N ) 14
Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AlwNI  (501)
1 site		 C A G N N N C T G G T C N N N G A C
Sticky ends from different AlwNI sites may not be compatible.
BmrI  (489)
1 site		 A C T G G G ( N ) 4 N T G A C C C ( N ) 4
The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
BmgBI  (489)
1 site		 C A C G T C G T G C A G
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BbvCI  (478)
1 site		 C C T C A G C G G A G T C G
Bpu10I  (478)
1 site		 C C T N A G C G G A N T C G
Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BglI  (350)
1 site		 G C C N N N N N G G C C G G N N N N N C C G
Sticky ends from different BglI sites may not be compatible.
EciI  (331)
1 site		 G G C G G A ( N ) 9 N N C C G C C T ( N ) 9
Sticky ends from different EciI sites may not be compatible.
PpuMI  (293)
1 site		 R G G W C C Y Y C C W G G R
Sticky ends from different PpuMI sites may not be compatible.
EcoO109I  (293)
1 site		 R G G N C C Y Y C C N G G R
Sticky ends from different EcoO109I sites may not be compatible.
StuI  (265)
1 site		 A G G C C T T C C G G A
BstAPI  (218)
1 site		 G C A N N N N N T G C C G T N N N N N A C G
Sticky ends from different BstAPI sites may not be compatible.
BclI  (143)
1 site		 T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BsrFI  (103)
1 site		 R C C G G Y Y G G C C R
Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BaeGI  (103)
1 site		 G K G C M C C M C G K G
Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (103)
1 site		 G K G C M C C M C G K G
Sticky ends from different Bme1580I sites may not be compatible.
BssSI  (76)
1 site		 C A C G A G G T G C T C
BssSαI  (76)
1 site		 C A C G A G G T G C T C
FspI  (40)
1 site		 T G C G C A A C G C G T
FspAI  (40)
1 site		 R T G C G C A Y Y A C G C G T R
BtgZI  (9)
1 site		 G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4
Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
Start  (0)
0 sites
hmMiCy1
1 .. 699  =  699 bp
232 amino acids  =  26.0 kDa
Product: humanized monomeric Midoriishi-Cyan fluorescent protein
mammalian codon-optimized
hmMiCy1
1 .. 699  =  699 bp
232 amino acids  =  26.0 kDa
Product: humanized monomeric Midoriishi-Cyan fluorescent protein
mammalian codon-optimized
ORF:  1 .. 699  =  699 bp
ORF:  232 amino acids  =  26.0 kDa
ORF:  3 .. 698  =  696 bp
ORF:  232 amino acids  =  25.9 kDa  (no start codon)
ORF:  1 .. 699  =  699 bp
ORF:  233 amino acids  =  22.7 kDa  (no start codon)
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ecliptic pHluorin
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phKikGR1-S1
pTurboYFP-B
FAPalpha2
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phKO1-S1
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mScarlet
phmAG1-S1
pTurboYFP-N
Fast-FT
mScarlet-2I
phMGFP
pTurboYFP-PRL
Fluorescent Timer
mScarlet-H
phmKeima-Red-S1
pZsGreen
FusionRed
mScarlet-I
phmKO1-S1
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Gamillus
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phmUkG1-S1
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GFP
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pZsGreen1-N1
GFPuv
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HcRed1
mStayGold (E138D)
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hdKeima570
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ratiometric pHluorin
hKikGR1
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pKatushka2S-C
Rhacostoma GFP
hKO
mTFP1
pKatushka2S-N
rsEGFP
hmAzami-Green
mTurquoise
pKikGR1-S1
rsEGFP2
hMGFP
mTurquoise2
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rsFusionRed1
hmKeima-Red
mUkG1
pKillerOrange-C
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hmKeima8.5
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PA-GFP
pKillerRed-C
SBFP2
hmKO2
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SCFP3A
hmMiCy1
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SiriusGFP
hrGFP
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Skylan-NS
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IFP1.4
pAcGFP1-C2
pLSSmOrange-N1
smURFP
IFP2.0
pAcGFP1-C3
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StayGold
iRFP670
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super-ecliptic pHluorin
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pmCherry
superfolder GFP
iRFP702
pAcGFP1-N2
pmCherry-1
SuperNova
iRFP713
pAcGFP1-N3
pmCherry-C1
SuperNova2
iRFP720
pAG-S1
pmCherry-N1
SYFP2
Kaede
PAmCherry
pMFAPalpha2
TagBFP
Katushka2S
PAmCherry1
pMFAPbeta1
TagCFP
KikGR1
pAmCyan
pMiCy1-S1
TagGFP2
KillerOrange
pAmCyan1-C1
pmKate2-C
TagRFP
KillerRed
pAmCyan1-N1
pmKate2-N
TagRFP-T
Kohinoor
PAmKate
pmKeima-Red-S1
TagRFP657
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pAsRed2
pmKikGR1-S1
TagYFP
LanYFP
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pmKO1-S1
TDsmURFP
LSSmKate1
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tdTomato
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